CHAIN A ENGINEERED MUTATION ARG200ALA GLY, SER, HIS INSERTED AT THE N-TERMINUS
配列の詳細
SAMPLE COMTAINS SER183, ILE184, ASP190, AND A ILE191 (CONFIRMED BY THE DNA SEQUENCE AND ELECTRON ...SAMPLE COMTAINS SER183, ILE184, ASP190, AND A ILE191 (CONFIRMED BY THE DNA SEQUENCE AND ELECTRON DENSITY OF SIDE-CHAINS). POSSIBLE ERROR IN SWISSPROT ENTRY.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.62 Å3/Da / 溶媒含有率: 53.13 %
結晶化
温度: 293 K / 手法: 蒸気拡散法 / pH: 8 詳細: VAPOR DIFFUSION AT 293 K MIXING 2 MICROLITERS OF A SOLUTION CONTAINING THE ENZYME, NUCLEOTIDES, AND 50 MM MGCL2 WITH 2 MICROLITERS OF A SOLUTION CONTAINING 21% PEG 3350, 100 MM TRIS HCL PH 8. ...詳細: VAPOR DIFFUSION AT 293 K MIXING 2 MICROLITERS OF A SOLUTION CONTAINING THE ENZYME, NUCLEOTIDES, AND 50 MM MGCL2 WITH 2 MICROLITERS OF A SOLUTION CONTAINING 21% PEG 3350, 100 MM TRIS HCL PH 8.0 AND 50 MICROLITERS OF DEAD SEA WATER.
モノクロメーター: YES / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9076 Å / 相対比: 1
反射
解像度: 1.7→35.3 Å / Num. obs: 27940 / % possible obs: 97.4 % / 冗長度: 5.3 % / Rsym value: 0.033 / Net I/σ(I): 24.2
反射 シェル
解像度: 1.7→1.8 Å / 冗長度: 4.3 % / Mean I/σ(I) obs: 5.3 / Rsym value: 0.222 / % possible all: 89.3
反射
*PLUS
Num. measured all: 148714 / Rmerge(I) obs: 0.033
反射 シェル
*PLUS
% possible obs: 89.3 % / Rmerge(I) obs: 0.222
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解析
ソフトウェア
名称
分類
REFMAC
精密化
XDS
データ削減
XSCALE
データスケーリング
精密化
構造決定の手法: 分子置換 / 解像度: 1.7→35.3 Å / 交差検証法: THROUGHOUT / σ(F): 0 詳細: TP5A IS A BISUBSTRATE INHIBITOR THAT CAN BE THOUGHT OF A CONSISTING OF ATP AND TMP JOINED BY A LINKER PHOSPHATE. THE LATTER IS NOT VISIBLE IN THE ELECTRON DENSITY MAP. THEREFORE, WE MODELED ...詳細: TP5A IS A BISUBSTRATE INHIBITOR THAT CAN BE THOUGHT OF A CONSISTING OF ATP AND TMP JOINED BY A LINKER PHOSPHATE. THE LATTER IS NOT VISIBLE IN THE ELECTRON DENSITY MAP. THEREFORE, WE MODELED THE NUCLEOTIDE AS ATP AND TMP.