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- PDB-1d6y: CRYSTAL STRUCTURE OF E. COLI COPPER-CONTAINING AMINE OXIDASE ANAE... -

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Basic information

Entry
Database: PDB / ID: 1d6y
TitleCRYSTAL STRUCTURE OF E. COLI COPPER-CONTAINING AMINE OXIDASE ANAEROBICALLY REDUCED WITH BETA-PHENYLETHYLAMINE AND COMPLEXED WITH NITRIC OXIDE.
ComponentsCOPPER AMINE OXIDASE
KeywordsOXIDOREDUCTASE / REACTION INTERMEDIATE MIMIC
Function / homology
Function and homology information


phenylethylamine catabolic process / aliphatic-amine oxidase activity / phenethylamine:oxygen oxidoreductase (deaminating) activity / aminoacetone:oxygen oxidoreductase(deaminating) activity / tryptamine:oxygen oxidoreductase (deaminating) activity / primary-amine oxidase / L-phenylalanine catabolic process / primary amine oxidase activity / amine metabolic process / quinone binding ...phenylethylamine catabolic process / aliphatic-amine oxidase activity / phenethylamine:oxygen oxidoreductase (deaminating) activity / aminoacetone:oxygen oxidoreductase(deaminating) activity / tryptamine:oxygen oxidoreductase (deaminating) activity / primary-amine oxidase / L-phenylalanine catabolic process / primary amine oxidase activity / amine metabolic process / quinone binding / copper ion binding / periplasmic space / calcium ion binding
Similarity search - Function
Copper amine oxidase-like, N-terminal domain / Copper amine oxidase-like, N-terminal domain superfamily / Copper amine oxidase-like, N-terminal / Copper amine oxidase N-terminal domain / Copper Amine Oxidase; Chain A, domain 1 / Copper amine oxidase, N2-terminal / Copper amine oxidase, N2 domain / Copper amine oxidase copper-binding site signature. / Copper amine oxidase topaquinone signature. / Copper amine oxidase, N3 domain ...Copper amine oxidase-like, N-terminal domain / Copper amine oxidase-like, N-terminal domain superfamily / Copper amine oxidase-like, N-terminal / Copper amine oxidase N-terminal domain / Copper Amine Oxidase; Chain A, domain 1 / Copper amine oxidase, N2-terminal / Copper amine oxidase, N2 domain / Copper amine oxidase copper-binding site signature. / Copper amine oxidase topaquinone signature. / Copper amine oxidase, N3 domain / Copper amine oxidase, N3-terminal / Copper amine oxidase, catalytic domain / Copper amine oxidase, N-terminal / Copper amine oxidase, catalytic domain superfamily / Copper amine oxidase, catalytic domain / Copper amine oxidase / Copper amine oxidase, enzyme domain / Nuclear Transport Factor 2; Chain: A, - #40 / Beta-galactosidase; Chain A, domain 5 / Nuclear Transport Factor 2; Chain: A, / Distorted Sandwich / Roll / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
COPPER (II) ION / PHENYLACETALDEHYDE / 2-PHENYLETHYLAMINE / NITRIC OXIDE / Primary amine oxidase
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.4 Å
AuthorsWilmot, C.M. / Hajdu, J. / McPherson, M.J. / Knowles, P.F. / Phillips, S.E.V.
Citation
Journal: Science / Year: 1999
Title: Visualization of dioxygen bound to copper during enzyme catalysis.
Authors: Wilmot, C.M. / Hajdu, J. / McPherson, M.J. / Knowles, P.F. / Phillips, S.E.
#1: Journal: Biochemistry / Year: 1999
Title: The Active Site Base Controls Cofactor Reactivity in Escherichia coli Amine Oxidase: X-ray Crystallographic Studies with Mutational Variants.
Authors: Murray, J.M. / Saysell, C.G. / Wilmot, C.M. / Tambyrajah, W.S. / Jaeger, J. / Knowles, P.F. / Phillips, S.E. / McPherson, M.J.
#2: Journal: Biochemistry / Year: 1997
Title: Catalytic Mechanism of the Quinoenzyme Amine Oxidase from Escherichia coli: Exploring the Reductive Half-Reaction.
Authors: Wilmot, C.M. / Murray, J.M. / Alton, G. / Parsons, M.R. / Convery, M.A. / Blakeley, V. / Corner, A.S. / Palcic, M.M. / Knowles, P.F. / McPherson, M.J. / Knowles, P.F.
#3: Journal: Structure / Year: 1995
Title: Crystal Structure of a Quinoenzyme: Copper Amine Oxidase of Escherichia coli at 2 Angstroms Resolution.
Authors: Parsons, M.R. / Convery, M.A. / Wilmot, C.M. / Yadav, K.D.S. / Blakeley, V. / Corner, A.S. / Phillips, S.E. / McPherson, M.J. / Knowles, P.F.
History
DepositionOct 16, 1999Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 2, 2000Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Non-polymer description / Version format compliance
Revision 1.3Jan 31, 2018Group: Experimental preparation / Category: exptl_crystal_grow / Item: _exptl_crystal_grow.temp

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: COPPER AMINE OXIDASE
B: COPPER AMINE OXIDASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)163,63015
Polymers162,7362
Non-polymers89413
Water25,7071427
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area16200 Å2
ΔGint-121 kcal/mol
Surface area50220 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)135.236, 166.482, 79.628
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein COPPER AMINE OXIDASE


Mass: 81367.758 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: UniProt: P46883, EC: 1.4.3.6

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Non-polymers , 7 types, 1440 molecules

#2: Chemical ChemComp-CU / COPPER (II) ION / Copper


Mass: 63.546 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cu
Details: Product produced anaerobically by enzyme reaction on beta-phenylethylamine substrate.
#3: Chemical
ChemComp-CA / CALCIUM ION / Calcium


Mass: 40.078 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Ca
#4: Chemical ChemComp-HY1 / PHENYLACETALDEHYDE / Phenylacetaldehyde


Mass: 120.149 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H8O
Details: Substrate of enzyme. Hydrochloride salt purchased from SIGMA
#5: Chemical ChemComp-NO / NITRIC OXIDE / Nitrogen monoxide / Nitric oxide


Mass: 30.006 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: NO
#6: Chemical ChemComp-PEA / 2-PHENYLETHYLAMINE / Phenethylamine


Mass: 122.188 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H12N / Comment: alkaloid*YM
#7: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H8O3
#8: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1427 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.75 Å3/Da / Density % sol: 55.31 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.2
Details: sodium citrate, HEPES buffer, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 18K
Crystal
*PLUS
Density % sol: 55 %
Crystal grow
*PLUS
pH: 7 / Method: vapor diffusion
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-ID
12.3 Mammonium sulfate1reservoir
2100 mMHEPES1reservoir

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: BM14 / Wavelength: 1.03
DetectorType: MARRESEARCH / Detector: IMAGE PLATE / Date: May 6, 1998
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.03 Å / Relative weight: 1
ReflectionResolution: 2.4→30 Å / Num. all: 65769 / Num. obs: 191118 / % possible obs: 92.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.9 % / Biso Wilson estimate: 46.1 Å2 / Rmerge(I) obs: 0.074 / Net I/σ(I): 16
Reflection shellResolution: 2.4→2.44 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.265 / % possible all: 92.5
Reflection
*PLUS
Num. obs: 65769 / Num. measured all: 191118
Reflection shell
*PLUS
% possible obs: 92.5 %

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Processing

Software
NameClassification
CNSrefinement
DENZOdata reduction
SCALEPACKdata scaling
CNSphasing
RefinementResolution: 2.4→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh and Huber
RfactorNum. reflectionSelection details
Rfree0.231 2274 By comparison to a complete dummy reflection set to 1.8 Angstroms for the cell and space group with the reflections randomly flagged to give a minimum of 2000 reflections in the test set at 2.6 Angstroms. This gave a percentage of 3.6% of reflections, which was the overall percent value in the test set to 1.8 Angstroms.
Rwork0.181 --
all0.183 65743 -
obs0.183 65743 -
Refinement stepCycle: LAST / Resolution: 2.4→30 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11347 0 49 1427 12823
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_angle_deg1.5
X-RAY DIFFRACTIONc_bond_d0.006
Software
*PLUS
Name: 'CNS' / Classification: refinement
Refinement
*PLUS
Rfactor obs: 0.181
Solvent computation
*PLUS
Displacement parameters
*PLUS

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