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- PDB-1cnm: ENHANCEMENT OF CATALYTIC EFFICIENCY OF PROTEINASE K THROUGH EXPOS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1cnm | ||||||
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Title | ENHANCEMENT OF CATALYTIC EFFICIENCY OF PROTEINASE K THROUGH EXPOSURE TO ANHYDROUS ORGANIC SOLVENT AT 70 DEGREES CELSIUS | ||||||
![]() | PROTEIN (PROTEINASE K) | ||||||
![]() | HYDROLASE / PROTEINASE K / ANHYDROUS ORGANIC SOLVENTS / STABILITY / ENHANCED ACTIVITY | ||||||
Function / homology | ![]() peptidase K / serine-type endopeptidase activity / proteolysis / extracellular space / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gupta, M.N. / Tyagi, R. / Sharma, S. / Karthikeyan, S. / Singh, T.P. | ||||||
![]() | ![]() Title: Enhancement of catalytic efficiency of enzymes through exposure to anhydrous organic solvent at 70 degrees C. Three-dimensional structure of a treated serine proteinase at 2.2 A resolution. Authors: Gupta, M.N. / Tyagi, R. / Sharma, S. / Karthikeyan, S. / Singh, T.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67 KB | Display | ![]() |
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PDB format | ![]() | 48.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373.4 KB | Display | ![]() |
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Full document | ![]() | 380.3 KB | Display | |
Data in XML | ![]() | 8.2 KB | Display | |
Data in CIF | ![]() | 12.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2prkS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28930.783 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() | ||||
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#2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.6 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 Details: CRYSTALLIZED BY DISSOLVING THE 10%(W/V) OF LYOPHILIZED ENZYME IN 50MM TRIS.HCL, 1MM CACL2, PH 6.5. 25 MICRO LITRE DROPS OF THIS SOLUTION WERE EQUILIBRATED IN A MICRODIALYSIS SETUP AGAINST 1M ...Details: CRYSTALLIZED BY DISSOLVING THE 10%(W/V) OF LYOPHILIZED ENZYME IN 50MM TRIS.HCL, 1MM CACL2, PH 6.5. 25 MICRO LITRE DROPS OF THIS SOLUTION WERE EQUILIBRATED IN A MICRODIALYSIS SETUP AGAINST 1M NANO3 IN THE SAME BUFFER AT 4 DEGREE., pH 7.5 | ||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.5 / Method: microdialysis | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 285 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 1, 1998 / Details: PIN HOLE |
Radiation | Monochromator: GRAPHITE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→15 Å / Num. obs: 12156 / % possible obs: 97 % / Observed criterion σ(I): -3 / Redundancy: 5.4 % / Rmerge(I) obs: 0.045 / Rsym value: 0.045 / Net I/σ(I): 40.01 |
Reflection shell | Resolution: 2.2→3 Å / Redundancy: 4.6 % / Rmerge(I) obs: 0.087 / Mean I/σ(I) obs: 24.41 / Rsym value: 0.087 / % possible all: 94 |
Reflection | *PLUS Num. measured all: 89243 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2PRK Resolution: 2.2→15 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 17.1 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→15 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 15 Å / σ(F): 0 / % reflection Rfree: 3 % / Rfactor obs: 0.168 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 17.1 Å2 |