Mass: 18.015 Da / Num. of mol.: 295 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THE SEQUENCE OF CHOLESTEROL OXIDASE FROM STREPTOMYCES SP FOUND IN THE SEQUENCE DATA BASE IS A ...THE SEQUENCE OF CHOLESTEROL OXIDASE FROM STREPTOMYCES SP FOUND IN THE SEQUENCE DATA BASE IS A PRECURSOR FORM OF THE ENZYME. THE MATURE ENZYME RUNS FROM RESIDUES 43 - 546. THE NUMBERING OF THE RESIDUES DIFFERS FROM THAT IN THE DATABASE DUE TO THIS DESCREPANCY IN THE SEQUENCE. IN ADDITION, THE NUMBERING HAS BEEN ADJUSTED TO CONFORM TO THAT FOUND IN THE STRUCTURE OF CHOLESTEROL OXIDASE FROM BREVIBACTERIUM STEROLICUM (ACCESSION CODE 3COX). THIS CHANGE IN THE NUMBERING SCHEME FACILITATES COMPARISONS OF THE TWO STRUCTURES. HIS447 HAS BEEN MUTATED TO A ASPARAGINE.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.2 Å3/Da / Density % sol: 44 %
Crystal grow
pH: 5.2 Details: CRYSTALLIZATION CONDITIONS: VAPOUR DIFFUSION USING THE HANGING DROP TECHNIQUE, PRECIPITANT CONDITIONS: 10-12% PEG 8000, 100MM SODIUM CACODYLATE PH 5.2, 75MM MNSO4 PROTEIN CONCENTRATION 8.5MG/ML
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