+Open data
-Basic information
Entry | Database: PDB / ID: 1c3c | ||||||
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Title | T. MARITIMA ADENYLOSUCCINATE LYASE | ||||||
Components | PROTEIN (ADENYLOSUCCINATE LYASE) | ||||||
Keywords | LYASE / PURINE BIOSYNTHESIS | ||||||
Function / homology | Function and homology information adenylosuccinate lyase / N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity / (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity / 'de novo' AMP biosynthetic process / 'de novo' IMP biosynthetic process / cytosol Similarity search - Function | ||||||
Biological species | Thermotoga maritima (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.8 Å | ||||||
Authors | Toth, E.A. / Yeates, T.O. | ||||||
Citation | Journal: Structure Fold.Des. / Year: 2000 Title: The structure of adenylosuccinate lyase, an enzyme with dual activity in the de novo purine biosynthetic pathway. Authors: Toth, E.A. / Yeates, T.O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1c3c.cif.gz | 358 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1c3c.ent.gz | 290.8 KB | Display | PDB format |
PDBx/mmJSON format | 1c3c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c3/1c3c ftp://data.pdbj.org/pub/pdb/validation_reports/c3/1c3c | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 49687.832 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermotoga maritima (bacteria) / Plasmid: PT7-7 / Production host: Escherichia coli (E. coli) References: UniProt: Q9X0I0, GenBank: 4981640, adenylosuccinate lyase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.25 Å3/Da / Density % sol: 62.2 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.5 Details: 100 MM NAACETATE, 0.5% PEG 4000, 6% GLYCEROL, pH 4.50 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X8C / Wavelength: 1 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 19, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→49.4 Å / Num. obs: 117163 / % possible obs: 97.8 % / Observed criterion σ(I): -3 / Redundancy: 4 % / Biso Wilson estimate: 16.33 Å2 / Rmerge(I) obs: 0.059 / Net I/σ(I): 21.67 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.295 / Mean I/σ(I) obs: 3.1 / % possible all: 84.1 |
Reflection shell | *PLUS % possible obs: 84.1 % |
-Processing
Software |
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Refinement | Resolution: 1.8→50 Å
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Refinement step | Cycle: LAST / Resolution: 1.8→50 Å
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Refine LS restraints |
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