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- PDB-1bs4: PEPTIDE DEFORMYLASE AS ZN2+ CONTAINING FORM (NATIVE) IN COMPLEX W... -

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Basic information

Entry
Database: PDB / ID: 1bs4
TitlePEPTIDE DEFORMYLASE AS ZN2+ CONTAINING FORM (NATIVE) IN COMPLEX WITH INHIBITOR POLYETHYLENE GLYCOL
ComponentsPROTEIN (PEPTIDE DEFORMYLASE)
KeywordsHYDROLASE / COMPLEX(ENZYME-INHIBITOR) / IRON METALLOPROTEASE / PROTEIN SYNTHESIS
Function / homology
Function and homology information


co-translational protein modification / N-terminal protein amino acid modification / peptide deformylase / peptide deformylase activity / ferrous iron binding / ribosome binding / hydrolase activity / translation / zinc ion binding / cytosol
Peptide deformylase / Peptide deformylase superfamily / Polypeptide deformylase
Peptide deformylase
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.9 Å
AuthorsBecker, A. / Schlichting, I. / Kabsch, W. / Groche, D. / Schultz, S. / Wagner, A.F.V.
Citation
Journal: Nat.Struct.Biol. / Year: 1998
Title: Iron center, substrate recognition and mechanism of peptide deformylase.
Authors: Becker, A. / Schlichting, I. / Kabsch, W. / Groche, D. / Schultz, S. / Wagner, A.F.
#1: Journal: J.Biol.Chem. / Year: 1998
Title: Structure of Peptide Deformylase and Identification of the Substrate Binding Site
Authors: Becker, A. / Schlichting, I. / Kabsch, W. / Schultz, S. / Wagner, A.F.V.
#2: Journal: Biochem.Biophys.Res.Commun. / Year: 1998
Title: Isolation and Crystallization of Functionally Competent Escherichia Coli Peptide Deformylase Forms Containing Either Iron or Nickel in the Active Site
Authors: Groche, D. / Becker, A. / Schlichting, I. / Kabsch, W. / Schultz, S. / Wagner, A.F.V.
Validation Report
SummaryFull reportAbout validation report
History
DepositionSep 1, 1998Deposition site: BNL / Processing site: RCSB
Revision 1.0Aug 27, 1999Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: PROTEIN (PEPTIDE DEFORMYLASE)
B: PROTEIN (PEPTIDE DEFORMYLASE)
C: PROTEIN (PEPTIDE DEFORMYLASE)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)59,31111
Polymers57,6793
Non-polymers1,6328
Water3,801211
1
A: PROTEIN (PEPTIDE DEFORMYLASE)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)19,7063
Polymers19,2261
Non-polymers4802
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: PROTEIN (PEPTIDE DEFORMYLASE)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)19,8024
Polymers19,2261
Non-polymers5763
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: PROTEIN (PEPTIDE DEFORMYLASE)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)19,8024
Polymers19,2261
Non-polymers5763
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
γ
α
β
Length a, b, c (Å)140.700, 63.300, 86.800
Angle α, β, γ (deg.)90.00, 120.60, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein/peptide PROTEIN (PEPTIDE DEFORMYLASE) / E.C.3.5.1.31 / PDF


Mass: 19226.248 Da / Num. of mol.: 3
Details: PDF PROTEIN FROM ESCHERICHIA COLI IS CRYSTALLIZED AS ZN2+ CONTAINING FORM, COCRYSTALLIZED WITH ...PDF PROTEIN FROM ESCHERICHIA COLI IS CRYSTALLIZED AS ZN2+ CONTAINING FORM, COCRYSTALLIZED WITH INHIBITOR POLYETHYLENE GLYCOL (PEG)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: JM109 / Cellular location: CYTOPLASMA / Gene: DEF / Plasmid: P925C / Gene (production host): DEF / Production host: Escherichia coli (E. coli) / Strain (production host): JM109 / References: UniProt: P0A6K3, EC: 3.5.1.31
#2: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Zn / Zinc
#3: Chemical ChemComp-2PE / NONAETHYLENE GLYCOL


Mass: 414.488 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C18H38O10
#4: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4 / Sulfate
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 211 / Source method: isolated from a natural source / Formula: H2O / Water

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 5

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Sample preparation

CrystalDensity Matthews: 2.88 Å3/Da / Density % sol: 57 %
Crystal growpH: 7.4
Details: REFERENCE: D.GROCHE,A.BECKER,I.SCHLICHTING,W.KABSCH, S.SCHULTZ,A.F.V.WAGNER ( 1998) BIOCHEM.BIOPHYS.RES.COMM. 246, 342, pH 7.4
Crystal grow
*PLUS
Method: vapor diffusion, hanging drop
Details: Groche, D., (1998) Biochem.Biophys.Res.Comm., 246, 342.
Components of the solutions
*PLUS

Crystal-ID: 1

IDConc.Common nameSol-ID
12 Mammonium sulfatereservoir
20.2 MMOPS-NH3reservoir
310 mMTCEPreservoir
430 mg/mlPDFdrop
51 %(w/v)PEG1000drop

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Data collection

DiffractionMean temperature: 293 K
Diffraction sourceSource: ROTATING ANODE / Type: ELLIOTT GX-18 / Wavelength: 1.5418
DetectorType: SIEMENS-NICOLET X100 / Detector: AREA DETECTOR / Date: Jun 15, 1997 / Details: FRANKS DOUBLE-MIRROR
RadiationMonochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 1.9→20 Å / Num. obs: 51676 / % possible obs: 99.2 % / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Biso Wilson estimate: 21.2 Å2 / Rmerge(I) obs: 0.048 / Net I/σ(I): 16.63
Reflection shellResolution: 1.9→2.01 Å / Redundancy: 2 % / Rmerge(I) obs: 0.162 / Mean I/σ(I) obs: 2.8 / % possible all: 98.4
Reflection
*PLUS
Num. measured all: 188577

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Processing

Software
NameVersionClassification
X-PLOR3.851model building
X-PLOR3.851refinement
XDSdata reduction
XSCALEdata scaling
X-PLOR3.851phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1ICJ
Resolution: 1.9→6 Å / Rfactor Rfree error: 0.003 / Data cutoff high absF: 100000 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
RfactorNum. reflection% reflectionSelection details
Rfree0.24 4916 10.1 %RANDOM
Rwork0.193 ---
Obs0.193 48788 99.5 %-
Displacement parametersBiso mean: 28.5 Å2
Refine analyze
FreeObs
Luzzati coordinate error0.26 Å0.22 Å
Luzzati d res low-6 Å
Luzzati sigma a0.18 Å0.21 Å
Refinement stepCycle: LAST / Resolution: 1.9→6 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4122 0 100 208 4430
Refine LS restraints

Refinement-ID: X-RAY DIFFRACTION

TypeDev idealDev ideal target
x_bond_d0.01
x_bond_d_na
x_bond_d_prot
x_angle_d
x_angle_d_na
x_angle_d_prot
x_angle_deg1.1
x_angle_deg_na
x_angle_deg_prot
x_dihedral_angle_d25.2
x_dihedral_angle_d_na
x_dihedral_angle_d_prot
x_improper_angle_d1.91
x_improper_angle_d_na
x_improper_angle_d_prot
x_mcbond_it1.051.5
x_mcangle_it1.732
x_scbond_it2.162
x_scangle_it3.532.5
LS refinement shellResolution: 1.9→2.01 Å / Rfactor Rfree error: 0.01 / Total num. of bins used: 6
RfactorNum. reflection% reflection
Rfree0.274 755 9.8 %
Rwork0.257 6910 -
Obs--98.6 %
Xplor file

Refinement-ID: X-RAY DIFFRACTION

Serial noParam fileTopol file
1PDFZNPEG.PARMPDFZNPEG.TOP
2PDFZNPEG.SCATTER
Software
*PLUS
Name: X-PLOR / Version: 3.851 / Classification: refinement
Refinement
*PLUS
Lowest resolution: 6 Å / σ(F): 0 / % reflection Rfree: 10.1 % / Rfactor Rfree: 0.24
Displacement parameters
*PLUS
Biso mean: 28.5 Å2
Refine LS restraints
*PLUS

Refinement-ID: X-RAY DIFFRACTION

TypeDev idealDev ideal target
x_angle_deg1.1
x_dihedral_angle_d
x_dihedral_angle_deg25.2
x_improper_angle_d
x_improper_angle_deg1.91
x_mcbond_it1.5
x_scbond_it2
x_mcangle_it2
x_scangle_it2.5
LS refinement shell
*PLUS
Rfactor Rfree: 0.274 / % reflection Rfree: 9.8 % / Rfactor Rwork: 0.257

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