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Yorodumi- PDB-1azn: CRYSTAL STRUCTURE OF THE AZURIN MUTANT PHE114ALA FROM PSEUDOMONAS... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1azn | ||||||
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Title | CRYSTAL STRUCTURE OF THE AZURIN MUTANT PHE114ALA FROM PSEUDOMONAS AERUGINOSA AT 2.6 ANGSTROMS RESOLUTION | ||||||
Components | AZURIN | ||||||
Keywords | ELECTRON TRANSPORT(COPPER BINDING) | ||||||
Function / homology | Function and homology information transition metal ion binding / periplasmic space / electron transfer activity / copper ion binding / zinc ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.6 Å | ||||||
Authors | Tsai, L.-C. / Sjolin, L. / Langer, V. / Pascher, T. / Nar, H. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1995 Title: Structure of the azurin mutant Phe114Ala from Pseudomonas aeruginosa at 2.6 A resolution. Authors: Tsai, L.C. / Sjolin, L. / Langer, V. / Pascher, T. / Nar, H. #1: Journal: J.Mol.Biol. / Year: 1991 Title: X-Ray Crystal Structure of the Two Site-Specific Mutants His35Gln and His35Leu of Azurin from Pseudomonas Aeruginosa Authors: Nar, H. / Messerschmidt, A. / Huber, R. / Van De Kamp, M. / Canters, G.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1azn.cif.gz | 117.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1azn.ent.gz | 90.7 KB | Display | PDB format |
PDBx/mmJSON format | 1azn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1azn_validation.pdf.gz | 394.6 KB | Display | wwPDB validaton report |
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Full document | 1azn_full_validation.pdf.gz | 427.7 KB | Display | |
Data in XML | 1azn_validation.xml.gz | 15.3 KB | Display | |
Data in CIF | 1azn_validation.cif.gz | 25.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/az/1azn ftp://data.pdbj.org/pub/pdb/validation_reports/az/1azn | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 13885.702 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / References: UniProt: P00282 #2: Chemical | ChemComp-CU / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.46 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 297-298 K / pH: 6 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.6 Å / Num. all: 62110 / Num. obs: 14831 / % possible obs: 91 % / Rmerge F obs: 0.071 |
-Processing
Software |
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Refinement | Resolution: 2.6→8 Å /
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Refinement step | Cycle: LAST / Resolution: 2.6→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.185 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.74 |