+Open data
-Basic information
Entry | Database: PDB / ID: 1uri | ||||||
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Title | AZURIN MUTANT WITH MET 121 REPLACED BY GLN | ||||||
Components | AZURIN | ||||||
Keywords | ELECTRON TRANSPORT / COPPER / PERIPLASMIC | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Achromobacter denitrificans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.94 Å | ||||||
Authors | Romero, A. / Nar, H. / Huber, R. / Messerschmidt, A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1993 Title: X-ray analysis and spectroscopic characterization of M121Q azurin. A copper site model for stellacyanin. Authors: Romero, A. / Hoitink, C.W. / Nar, H. / Huber, R. / Messerschmidt, A. / Canters, G.W. #1: Journal: J.Mol.Biol. / Year: 1988 Title: Structure of Azurin from Alcaligenes Denitrificans Authors: Baker, E.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uri.cif.gz | 61.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uri.ent.gz | 48.9 KB | Display | PDB format |
PDBx/mmJSON format | 1uri.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ur/1uri ftp://data.pdbj.org/pub/pdb/validation_reports/ur/1uri | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 14004.936 Da / Num. of mol.: 2 / Mutation: M121Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Achromobacter denitrificans (bacteria) / Strain: JM101 / Plasmid: PCH5 / Production host: Escherichia coli (E. coli) / References: UniProt: P00280 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.97 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 5.2 / Method: vapor diffusion | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER / Date: Jun 2, 1991 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 16824 / % possible obs: 79 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.077 |
Reflection | *PLUS Highest resolution: 1.94 Å / Lowest resolution: 9999 Å / Num. measured all: 58815 |
Reflection shell | *PLUS Highest resolution: 1.94 Å / Lowest resolution: 1.99 Å / % possible obs: 27.2 % |
-Processing
Software |
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Refinement | Resolution: 1.94→8 Å / σ(F): 2 Details: BESIDES GLYCINE RESIDUES, ONLY WEAKLY DEFINED RESIDUES FROM THE N-TERMINAL (GLN 2) LIE OUTSIDE THE ALLOWED REGIONS IN THE RAMACHANDRAN PLOT.
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Displacement parameters | Biso mean: 21.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.94→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 1.94 Å / Lowest resolution: 1.99 Å / Rfactor all: 0.306 |