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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1ax8 | ||||||
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タイトル | Human obesity protein, leptin | ||||||
![]() | OBESITY PROTEIN | ||||||
![]() | CYTOKINE / HELICAL CYTOKINE / HEMATOPOIETIC FACTOR / DIABETES / OBESITY | ||||||
機能・相同性 | ![]() regulation of lipoprotein lipid oxidation / cellular response to L-ascorbic acid / positive regulation of fat cell apoptotic process / negative regulation of glutamine transport / negative regulation of appetite by leptin-mediated signaling pathway / negative regulation of glucagon secretion / regulation of endothelial cell proliferation / regulation of natural killer cell mediated cytotoxicity / regulation of natural killer cell proliferation / leptin receptor binding ...regulation of lipoprotein lipid oxidation / cellular response to L-ascorbic acid / positive regulation of fat cell apoptotic process / negative regulation of glutamine transport / negative regulation of appetite by leptin-mediated signaling pathway / negative regulation of glucagon secretion / regulation of endothelial cell proliferation / regulation of natural killer cell mediated cytotoxicity / regulation of natural killer cell proliferation / leptin receptor binding / regulation of bone remodeling / positive regulation of luteinizing hormone secretion / bone growth / regulation of natural killer cell activation / glycerol biosynthetic process / regulation of steroid biosynthetic process / elastin metabolic process / leptin-mediated signaling pathway / positive regulation of follicle-stimulating hormone secretion / positive regulation of monoatomic ion transport / regulation of intestinal cholesterol absorption / positive regulation of hepatic stellate cell activation / regulation of brown fat cell differentiation / positive regulation of peroxisome proliferator activated receptor signaling pathway / regulation of nitric-oxide synthase activity / adult feeding behavior / activation of protein kinase C activity / bone mineralization involved in bone maturation / sexual reproduction / negative regulation of cartilage development / ovulation from ovarian follicle / negative regulation of D-glucose import / negative regulation of appetite / positive regulation of developmental growth / energy reserve metabolic process / leukocyte tethering or rolling / bile acid metabolic process / cellular response to leptin stimulus / prostaglandin secretion / cardiac muscle hypertrophy / Signaling by Leptin / hormone metabolic process / positive regulation of p38MAPK cascade / cell surface receptor signaling pathway via STAT / intestinal absorption / insulin secretion / eating behavior / aorta development / negative regulation of vasoconstriction / regulation of gluconeogenesis / response to vitamin E / peptide hormone receptor binding / fatty acid beta-oxidation / regulation of cytokine production involved in inflammatory response / central nervous system neuron development / response to dietary excess / energy homeostasis / negative regulation of lipid storage / T cell differentiation / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of TOR signaling / cell surface receptor signaling pathway via JAK-STAT / regulation of angiogenesis / adipose tissue development / positive regulation of insulin receptor signaling pathway / phagocytosis / cholesterol metabolic process / cellular response to retinoic acid / positive regulation of T cell proliferation / positive regulation of interleukin-12 production / regulation of insulin secretion / negative regulation of autophagy / placenta development / response to activity / positive regulation of interleukin-8 production / determination of adult lifespan / positive regulation of receptor signaling pathway via JAK-STAT / female pregnancy / response to insulin / circadian rhythm / hormone activity / Transcriptional regulation of white adipocyte differentiation / lipid metabolic process / regulation of blood pressure / positive regulation of interleukin-6 production / positive regulation of protein import into nucleus / cellular response to insulin stimulus / glucose metabolic process / positive regulation of reactive oxygen species metabolic process / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / positive regulation of tumor necrosis factor production / glucose homeostasis / response to estradiol / positive regulation of cold-induced thermogenesis / response to ethanol / angiogenesis / response to hypoxia / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / regulation of cell cycle / positive regulation of MAPK cascade 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Zhang, F. / Beals, J.M. / Briggs, S.L. / Clawson, D.K. / Wery, J.-P. / Schevitz, R.W. | ||||||
![]() | ![]() タイトル: Crystal structure of the obese protein leptin-E100. 著者: Zhang, F. / Basinski, M.B. / Beals, J.M. / Briggs, S.L. / Churgay, L.M. / Clawson, D.K. / DiMarchi, R.D. / Furman, T.C. / Hale, J.E. / Hsiung, H.M. / Schoner, B.E. / Smith, D.P. / Zhang, X.Y. ...著者: Zhang, F. / Basinski, M.B. / Beals, J.M. / Briggs, S.L. / Churgay, L.M. / Clawson, D.K. / DiMarchi, R.D. / Furman, T.C. / Hale, J.E. / Hsiung, H.M. / Schoner, B.E. / Smith, D.P. / Zhang, X.Y. / Wery, J.P. / Schevitz, R.W. #1: ![]() タイトル: Erratum. Positional Cloning of the Mouse Obese Gene and its Human Homologue 著者: Zhang, Y. / Proenca, R. / Maffei, M. / Barone, M. / Leopold, L. / Friedman, J.M. #2: ![]() タイトル: Positional Cloning of the Mouse Obese Gene and its Human Homologue 著者: Zhang, Y. / Proenca, R. / Maffei, M. / Barone, M. / Leopold, L. / Friedman, J.M. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 39.5 KB | 表示 | ![]() |
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PDB形式 | ![]() | 27.4 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 411.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 413.7 KB | 表示 | |
XML形式データ | ![]() | 9.3 KB | 表示 | |
CIF形式データ | ![]() | 11.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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1 |
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2 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 15984.296 Da / 分子数: 1 / 変異: W100E / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: 水 | ChemComp-HOH / |
Has protein modification | Y |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.5 Å3/Da / 溶媒含有率: 65.4 % |
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結晶化 | pH: 7.5 詳細: PROTEIN WAS CRYSTALLIZED FROM 1.8M NA FORMATE, 100MM TRIS, PH 7.5 |
結晶化 | *PLUS 手法: unknown |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: RIGAKU / 検出器: IMAGE PLATE / 日付: 1996年9月1日 / 詳細: MIRRORS |
放射 | モノクロメーター: NI FILTER / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.4→15 Å / Num. obs: 8404 / % possible obs: 99.2 % / Observed criterion σ(I): 1 / 冗長度: 5.4 % / Rmerge(I) obs: 0.052 / Rsym value: 0.054 / Net I/σ(I): 14.5 |
反射 シェル | 解像度: 2.4→2.44 Å / 冗長度: 4.5 % / Rmerge(I) obs: 0.221 / Mean I/σ(I) obs: 8.5 / Rsym value: 0.263 / % possible all: 99.5 |
反射 | *PLUS Num. measured all: 45300 |
反射 シェル | *PLUS % possible obs: 99.5 % |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]()
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原子変位パラメータ | Biso mean: 39.3 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.4→8 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.4→2.51 Å / Rfactor Rfree error: 0.035 / Total num. of bins used: 8
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Xplor file |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS Rfactor obs: 0.322 |