+
データを開く
-
基本情報
登録情報 | データベース: PDB / ID: 1aui | ||||||
---|---|---|---|---|---|---|---|
タイトル | HUMAN CALCINEURIN HETERODIMER | ||||||
![]() | (SERINE/THREONINE PHOSPHATASE ...) x 2 | ||||||
![]() | HYDROLASE / PHOSPHATASE / IMMUNOSUPPRESSION | ||||||
機能・相同性 | ![]() negative regulation of angiotensin-activated signaling pathway / regulation of cell proliferation involved in kidney morphogenesis / calcium-dependent protein serine/threonine phosphatase regulator activity / positive regulation of glomerulus development / negative regulation of calcium ion import across plasma membrane / calcium-dependent protein serine/threonine phosphatase activity / protein serine/threonine phosphatase complex / negative regulation of signaling / positive regulation of saliva secretion / positive regulation of cardiac muscle hypertrophy in response to stress ...negative regulation of angiotensin-activated signaling pathway / regulation of cell proliferation involved in kidney morphogenesis / calcium-dependent protein serine/threonine phosphatase regulator activity / positive regulation of glomerulus development / negative regulation of calcium ion import across plasma membrane / calcium-dependent protein serine/threonine phosphatase activity / protein serine/threonine phosphatase complex / negative regulation of signaling / positive regulation of saliva secretion / positive regulation of cardiac muscle hypertrophy in response to stress / positive regulation of calcium ion import across plasma membrane / calmodulin-dependent protein phosphatase activity / calcineurin complex / positive regulation of connective tissue replacement / negative regulation of dendrite morphogenesis / calcineurin-mediated signaling / slit diaphragm / peptidyl-serine dephosphorylation / lung epithelial cell differentiation / calcineurin-NFAT signaling cascade / renal filtration / skeletal muscle tissue regeneration / regulation of synaptic vesicle cycle / transition between fast and slow fiber / positive regulation of calcineurin-NFAT signaling cascade / myelination in peripheral nervous system / positive regulation of osteoclast differentiation / cardiac muscle hypertrophy in response to stress / regulation of postsynaptic neurotransmitter receptor internalization / parallel fiber to Purkinje cell synapse / dendrite morphogenesis / cyclosporin A binding / myosin phosphatase activity / CLEC7A (Dectin-1) induces NFAT activation / branching involved in blood vessel morphogenesis / postsynaptic modulation of chemical synaptic transmission / extrinsic component of plasma membrane / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / positive regulation of activated T cell proliferation / positive regulation of endocytosis / Calcineurin activates NFAT / positive regulation of cell adhesion / DARPP-32 events / Activation of BAD and translocation to mitochondria / epidermis development / epithelial to mesenchymal transition / phosphatase binding / negative regulation of insulin secretion / multicellular organismal response to stress / positive regulation of osteoblast differentiation / skeletal muscle fiber development / dephosphorylation / keratinocyte differentiation / response to amphetamine / T cell activation / excitatory postsynaptic potential / hippocampal mossy fiber to CA3 synapse / FCERI mediated Ca+2 mobilization / protein dephosphorylation / cellular response to glucose stimulus / wound healing / Schaffer collateral - CA1 synapse / modulation of chemical synaptic transmission / sarcolemma / Z disc / response to calcium ion / G1/S transition of mitotic cell cycle / protein import into nucleus / calcium ion transport / Ca2+ pathway / heart development / ATPase binding / postsynapse / dendritic spine / protein dimerization activity / calmodulin binding / positive regulation of cell migration / protein domain specific binding / negative regulation of gene expression / glutamatergic synapse / calcium ion binding / protein-containing complex binding / positive regulation of gene expression / enzyme binding / positive regulation of transcription by RNA polymerase II / mitochondrion / nucleoplasm / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Kissinger, C.R. / Parge, H.E. / Knighton, D.R. / Pelletier, L.A. / Lewis, C.T. / Tempczyk, A. / Villafranca, J.E. | ||||||
![]() | ![]() タイトル: Crystal structures of human calcineurin and the human FKBP12-FK506-calcineurin complex. 著者: Kissinger, C.R. / Parge, H.E. / Knighton, D.R. / Lewis, C.T. / Pelletier, L.A. / Tempczyk, A. / Kalish, V.J. / Tucker, K.D. / Showalter, R.E. / Moomaw, E.W. / Gastinel, L.N. / Habuka, N. / ...著者: Kissinger, C.R. / Parge, H.E. / Knighton, D.R. / Lewis, C.T. / Pelletier, L.A. / Tempczyk, A. / Kalish, V.J. / Tucker, K.D. / Showalter, R.E. / Moomaw, E.W. / Gastinel, L.N. / Habuka, N. / Chen, X. / Maldonado, F. / Barker, J.E. / Bacquet, R. / Villafranca, J.E. | ||||||
履歴 |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-
ダウンロード
PDBx/mmCIF形式 | ![]() | 137.9 KB | 表示 | ![]() |
---|---|---|---|---|
PDB形式 | ![]() | 104.7 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 377.6 KB | 表示 | ![]() |
---|---|---|---|---|
文書・詳細版 | ![]() | 384.5 KB | 表示 | |
XML形式データ | ![]() | 12.4 KB | 表示 | |
CIF形式データ | ![]() | 21.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
-
リンク
-
集合体
登録構造単位 | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
単位格子 |
|
-
要素
-SERINE/THREONINE PHOSPHATASE ... , 2種, 2分子 AB
#1: タンパク質 | 分子量: 58756.676 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() 参照: UniProt: Q08209, protein-serine/threonine phosphatase |
---|---|
#2: タンパク質 | 分子量: 19191.709 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() 参照: UniProt: P63098, protein-serine/threonine phosphatase |
-非ポリマー , 4種, 442分子 ![](data/chem/img/ZN.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
#3: 化合物 | ChemComp-ZN / | ||
---|---|---|---|
#4: 化合物 | ChemComp-FE / | ||
#5: 化合物 | ChemComp-CA / #6: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
---|
-
試料調製
結晶 | マシュー密度: 2.8 Å3/Da / 溶媒含有率: 56 % | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
結晶化 | pH: 7.5 詳細: PROTEIN WAS CRYSTALLIZED FROM 8% PEG 6000, 0.1M CACL2, 0.1M TES PH 7.5, 1 MM DTT. | |||||||||||||||
結晶化 | *PLUS 手法: unknown | |||||||||||||||
溶液の組成 | *PLUS
|
-データ収集
回折 | 平均測定温度: 87 K |
---|---|
放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: FUJI / 検出器: IMAGE PLATE / 日付: 1994年10月1日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.918 Å / 相対比: 1 |
反射 | 解像度: 2.1→30 Å / Num. obs: 50007 / % possible obs: 92.5 % / Observed criterion σ(I): 0 / 冗長度: 4 % / Rsym value: 0.069 |
反射 | *PLUS Rmerge(I) obs: 0.069 |
-
解析
ソフトウェア |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 構造決定の手法: MIR PLUS ANOMALOUS SCATTERING / 解像度: 2.1→10 Å / Isotropic thermal model: UNRESTRAINED 詳細: SIDE CHAIN ATOMS WITHOUT DISCERNIBLE ELECTRON DENSITY HAVE BEEN MODELLED IN STEREOCHEMICALLY REASONABLE POSITIONS AND ASSIGNED OCCUPANCIES OF ZERO.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 39.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.1→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS σ(F): 0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
|