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- PDB-1apa: X-RAY STRUCTURE OF A POKEWEED ANTIVIRAL PROTEIN, CODED BY A NEW G... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1apa | ||||||
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Title | X-RAY STRUCTURE OF A POKEWEED ANTIVIRAL PROTEIN, CODED BY A NEW GENOMIC CLONE, AT 0.23 NM RESOLUTION. A MODEL STRUCTURE PROVIDES A SUITABLE ELECTROSTATIC FIELD FOR SUBSTRATE BINDING. | ||||||
![]() | POKEWEED ANTIVIRAL PROTEIN | ||||||
![]() | ANTIVIRAL PROTEIN / GENOMIC CLONE | ||||||
Function / homology | ![]() cell wall / rRNA N-glycosylase / rRNA N-glycosylase activity / toxin activity / defense response to virus / negative regulation of translation / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Ago, H. / Kataoka, J. / Tsuge, H. / Habuka, N. / Inagaki, E. / Noma, M. / Miyano, M. | ||||||
![]() | ![]() Title: X-ray structure of a pokeweed antiviral protein, coded by a new genomic clone, at 0.23 nm resolution. A model structure provides a suitable electrostatic field for substrate binding. Authors: Ago, H. / Kataoka, J. / Tsuge, H. / Habuka, N. / Inagaki, E. / Noma, M. / Miyano, M. #1: ![]() Title: Expression of a Pokeweed Antiviral Protein in Escherichia Coli and its Characterization Authors: Kataoka, J. / Ago, H. / Habuka, N. / Furuno, M. / Masuta, C. / Miyano, M. / Koiwai, A. #2: ![]() Title: Isolation and Analysis of a Genomic Clone Encoding a Pokeweed Antiviral Protein Authors: Kataoka, J. / Habuka, N. / Masuta, C. / Miyano, M. / Koiwai, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 59.1 KB | Display | ![]() |
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PDB format | ![]() | 47.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 366.2 KB | Display | ![]() |
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Full document | ![]() | 373.8 KB | Display | |
Data in XML | ![]() | 7.6 KB | Display | |
Data in CIF | ![]() | 11.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 52 |
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Components
#1: Protein | Mass: 29766.078 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() References: UniProt: Q03464 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.08 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: drop consists of equal amounts of protein and precipitant solutions | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.3 Å / Num. obs: 13157 / % possible obs: 98.9 % / Num. measured all: 21034 / Rmerge(I) obs: 0.068 |
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Processing
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Refinement | Rfactor Rwork: 0.172 / Rfactor obs: 0.172 / Highest resolution: 2.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.3 Å
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