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- PDB-1ah8: STRUCTURE OF THE ORTHORHOMBIC FORM OF THE N-TERMINAL DOMAIN OF TH... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ah8 | ||||||
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Title | STRUCTURE OF THE ORTHORHOMBIC FORM OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE | ||||||
![]() | HEAT SHOCK PROTEIN 90 | ||||||
![]() | CHAPERONE / ATP-BINDING / HEAT SHOCK | ||||||
Function / homology | ![]() The NLRP3 inflammasome / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / eNOS activation / Extra-nuclear estrogen signaling / VEGFR2 mediated vascular permeability / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / HSF1-dependent transactivation / HSF1 activation / response to oxygen levels / protein targeting to mitochondrion ...The NLRP3 inflammasome / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / eNOS activation / Extra-nuclear estrogen signaling / VEGFR2 mediated vascular permeability / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / HSF1-dependent transactivation / HSF1 activation / response to oxygen levels / protein targeting to mitochondrion / box C/D snoRNP assembly / regulation of telomere maintenance / response to osmotic stress / 'de novo' protein folding / protein maturation / proteasome assembly / Neutrophil degranulation / positive regulation of telomere maintenance via telomerase / ATP-dependent protein folding chaperone / disordered domain specific binding / unfolded protein binding / protein folding / cellular response to heat / protein refolding / protein stabilization / perinuclear region of cytoplasm / ATP hydrolysis activity / protein-containing complex / ATP binding / identical protein binding / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Prodromou, C. / Roe, S.M. / Pearl, L.H. | ||||||
![]() | ![]() Title: A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone. Authors: Prodromou, C. / Roe, S.M. / Piper, P.W. / Pearl, L.H. #1: ![]() Title: Expression and Crystallization of the Yeast Hsp82 Chaperone, and Preliminary X-Ray Diffraction Studies of the Amino-Terminal Domain Authors: Prodromou, C. / Piper, P.W. / Pearl, L.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 110.6 KB | Display | ![]() |
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PDB format | ![]() | 85 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 383.9 KB | Display | ![]() |
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Full document | ![]() | 391.3 KB | Display | |
Data in XML | ![]() | 11.3 KB | Display | |
Data in CIF | ![]() | 18.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24873.398 Da / Num. of mol.: 2 / Fragment: N-TERMINAL DOMAIN / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Chemical | ChemComp-GOL / #3: Water | ChemComp-HOH / | Sequence details | SIX RESIDUE HISTIDINE TAG USED FOR PURIFICATION WAS NOT REMOVED PRIOR TO CRYSTALLISATION. IT IS NOT ...SIX RESIDUE HISTIDINE TAG USED FOR PURIFICATI | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58 % | ||||||||||||||||||||||||||||||
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Crystal grow | Method: under oil / pH: 5 Details: THE PROTEIN WAS CRYSTALLISED UNDER OIL IN TERASAKI PLATES. THE DROPS CONTAINED 20.5MG ML PROTEIN, 9% PEGME 550, 60MM CALCIUM CHLORIDE, 25% GLYCEROL AND 30MM SODIUM ACETATE PH 5.0, under oil | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.4 / Method: unknownDetails: Prodromou, C., (1996) Proteins: Struct.,Funct., Genet., 25, 517. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 1, 1996 / Details: PT-COATED SI TORROIDAL MIRROR |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→25 Å / Num. obs: 34954 / % possible obs: 99.7 % / Observed criterion σ(I): 1 / Redundancy: 3.5 % / Biso Wilson estimate: 14.5 Å2 / Rmerge(I) obs: 0.033 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 2.1→2.15 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.033 / Mean I/σ(I) obs: 7.9 / Rsym value: 0.092 / % possible all: 92.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: TETRAGONAL FORM Resolution: 2.1→8 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 Details: BULK SOLVENT MODEL USED THIS IS THE ORTHORHOMBIC CRYSTAL FORM.
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Displacement parameters | Biso mean: 25.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.018 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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