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Open data
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Basic information
Entry | Database: PDB / ID: 1a9q | ||||||
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Title | BOVINE PURINE NUCLEOSIDE PHOSPHORYLASE COMPLEXED WITH INOSINE | ||||||
![]() | PURINE NUCLEOSIDE PHOSPHORYLASE | ||||||
![]() | PENTOSYLTRANSFERASE / PURINE NUCLEOSIDE PHOSPHORYLASE | ||||||
Function / homology | ![]() guanosine phosphorylase activity / purine-nucleoside phosphorylase / purine ribonucleoside salvage / purine-nucleoside phosphorylase activity / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Mao, C. / Cook, W.J. / Zhou, M. / Fedorov, A.A. / Almo, S.C. / Ealick, S.E. | ||||||
![]() | ![]() Title: Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues. Authors: Mao, C. / Cook, W.J. / Zhou, M. / Federov, A.A. / Almo, S.C. / Ealick, S.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.5 KB | Display | ![]() |
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PDB format | ![]() | 50.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 391.6 KB | Display | ![]() |
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Full document | ![]() | 395.3 KB | Display | |
Data in XML | ![]() | 7.5 KB | Display | |
Data in CIF | ![]() | 10.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1a9oC ![]() 1a9pC ![]() 1a9rC ![]() 1a9sC ![]() 1a9tC ![]() 1pbnSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 31428.799 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() References: UniProt: P55859, purine-nucleoside phosphorylase |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-HPA / |
#4: Water | ChemComp-HOH / |
Nonpolymer details | THE INOSINE SOAK RESULTS IN AN APPARENT HYDROLYSIS BY PURINE NUCLEOSIDE PHOSPHORYLASE. ONLY ...THE INOSINE SOAK RESULTS IN AN APPARENT HYDROLYSIS |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.48 % | ||||||||||||||||||||||||
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Crystal grow | Details: PROTEIN WAS CRYSTALLIZED FROM 31-35% PEG-400 IN 100 MM HEPES BUFFER, PH 7.8-8.2; 100 MM MGCL2; 1% OCTYL-BETA- D-GLUCOPYRANOSIDE. CRYSTAL SOAKED IN ARTI- FICIAL MOTHER LIQUOR CONTAINING 7.3 MM INOSINE. PH range: 7.8-8.2 | ||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 65 % | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / PH range low: 8.2 / PH range high: 7.8 | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 296 K |
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Diffraction source | Source: ![]() |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Aug 1, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→8 Å / Num. obs: 15990 / % possible obs: 89 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1PBN Resolution: 2→8 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 19 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.2 / Rfactor Rwork: 0.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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