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- EMDB-9269: Single-Molecule 3D Image of Low-Density Lipoprotein in Complex wi... -
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Open data
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Basic information
Entry | Database: EMDB / ID: 9269 |
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Title | Single-Molecule 3D Image of Low-Density Lipoprotein in Complex with Cholesteryl Ester Transfer Protein (No. 02) |
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![]() | Human plasma low-density lipoprotein in complex with cholesteryl ester transfer protein: Human plasma low-density lipoprotein / Cholesteryl ester transfer protein |
Source | ![]() ![]() |
Method | ![]() ![]() |
![]() | Wu H / Zhai X / Lei D / Liu J / Yu Y / Bie R / Ren G |
![]() | Journal: Sci Rep / Year: 2018 Title: An Algorithm for Enhancing the Image Contrast of Electron Tomography. ![]() |
Date | Deposition: Oct 27, 2018 / Header (metadata) release: Nov 28, 2018 / Map release: Nov 28, 2018 / Last update: Nov 28, 2018 |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
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Header (meta data in XML format) | ![]() ![]() ![]() |
FSC (resolution estimation) | ![]() |
Images | ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-9269 |
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Links
-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire Human plasma low-density lipoprotein in complex with cholesteryl ...
Entire | Name: Human plasma low-density lipoprotein in complex with cholesteryl ester transfer protein Details: Low-density lipoproteins (LDLs) were isolated from human plasma by density gradient ultracentrifugation (Children's Hospital Oakland Research Institute). Cholesteryl ester transfer protein (CETP) was produced by MERCK. LDL and CETP were incubated at 37 degC for 15 minutes at a molar ratio of ~4:1. Number of components: 3 |
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-Component #1: protein, Human plasma low-density lipoprotein in complex with cho...
Protein | Name: Human plasma low-density lipoprotein in complex with cholesteryl ester transfer protein Details: Low-density lipoproteins (LDLs) were isolated from human plasma by density gradient ultracentrifugation (Children's Hospital Oakland Research Institute). Cholesteryl ester transfer protein (CETP) was produced by MERCK. LDL and CETP were incubated at 37 degC for 15 minutes at a molar ratio of ~4:1. Recombinant expression: No |
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Mass | Theoretical: 2.5 MDa |
Source | Species: ![]() ![]() |
-Component #2: protein, Human plasma low-density lipoprotein
Protein | Name: Human plasma low-density lipoprotein Details: Low-density lipoproteins (LDLs) were isolated from human plasma by density gradient ultracentrifugation (Children's Hospital Oakland Research Institute). Recombinant expression: No |
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-Component #3: cellular-component, Cholesteryl ester transfer protein
Cellular-component | Name: Cholesteryl ester transfer protein Details: Cholesteryl ester transfer protein (CETP) was produced by MERCK. Recombinant expression: No |
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Source | Species: ![]() ![]() |
Source (natural) | Organ or tissue: Blood |
-Experimental details
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Sample preparation
Specimen | Specimen state: particle / Method: ![]() |
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Sample solution | Buffer solution: 1X Dulbeccos phosphate-buffered saline / pH: 7 |
Vitrification | Instrument: LEICA EM GP / Cryogen name: ETHANE / Humidity: 90 % |
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Electron microscopy imaging
![]() | Microscope: ZEISS LIBRA120PLUS |
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Electron gun | Electron source: LAB6 / Accelerating voltage: 120 kV / Electron dose: 0.26 e/Å2 / Illumination mode: FLOOD BEAM |
Lens | Magnification: 50000.0 X (nominal) / Cs: 2.2 mm / Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: OTHER |
Camera | Detector: GATAN ULTRASCAN 4000 (4k x 4k) |