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Open data
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Basic information
Entry | Database: EMDB / ID: 9247 |
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Title | Structure of full-length IP3R1 channel in ligand-bound state |
![]() | Cryo-EM densty map of IP3R1 in a ligand-bound state |
![]() | Inositol 1,4,5-trisphosphate receptor: inositol 1,4,5-trisphosphate receptor |
Source | ![]() ![]() ![]() |
Method | ![]() ![]() |
![]() | Fan G / Baker MR / Wang Z / Seryshev AB / Ludtke SJ / Baker ML / Serysheva II |
![]() | Journal: Cell Res. / Year: 2018 Title: Cryo-EM reveals ligand induced allostery underlying InsPR channel gating. ![]() |
Date | Deposition: Oct 22, 2018 / Header (metadata) release: Nov 7, 2018 / Map release: Dec 5, 2018 / Last update: Dec 12, 2018 |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
![]() | ![]() |
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Header (meta data in XML format) | ![]() ![]() ![]() |
Images | ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-9247 |
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Links
-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.26 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire Inositol 1,4,5-trisphosphate receptor
Entire | Name: Inositol 1,4,5-trisphosphate receptor / Details: tetrameric assembly / Number of components: 2 |
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-Component #1: protein, Inositol 1,4,5-trisphosphate receptor
Protein | Name: Inositol 1,4,5-trisphosphate receptor / Details: tetrameric assembly![]() |
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Mass | Theoretical: 1.3 MDa |
Source | Species: ![]() ![]() ![]() |
-Component #2: protein, inositol 1,4,5-trisphosphate receptor
Protein | Name: inositol 1,4,5-trisphosphate receptor / Recombinant expression: No |
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Source | Species: ![]() ![]() ![]() |
-Experimental details
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Sample preparation
Specimen | Specimen state: particle / Method: ![]() |
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Sample solution | Buffer solution: 50 mM Tris-HCl buffer (pH 7.4), 150 mM NaCl, 1 mM DTT, 0.4% CHAPS,100 nM of AdA, 300 nM of Ca2+, protease inhibitors pH: 7.4 |
Support film | unavailable |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 293 K / Humidity: 100 % |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Tecnai F30 / Image courtesy: FEI Company |
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![]() | Microscope: FEI TECNAI F30 |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Cs: 2 mm / Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: GATAN LIQUID NITROGEN / Temperature: K ( 93.0 - 93.0 K) |
Camera | Detector: GATAN K2 SUMMIT (4k x 4k) |
-Image acquisition
Image acquisition | Number of digital images: 14686 |
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Image processing
![]() | Method: ![]() |
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3D reconstruction | Algorithm: FOURIER SPACE / Software: RELION / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF |