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TitleCryo-EM reveals ligand induced allostery underlying InsPR channel gating.
Journal, issue, pagesCell Res, Vol. 28, Issue 12, Page 1158-1170, Year 2018
Publish dateNov 23, 2018
AuthorsGuizhen Fan / Mariah R Baker / Zhao Wang / Alexander B Seryshev / Steven J Ludtke / Matthew L Baker / Irina I Serysheva /
PubMed AbstractInositol-1,4,5-trisphosphate receptors (InsPRs) are cation channels that mobilize Ca from intracellular stores in response to a wide range of cellular stimuli. The paradigm of InsPR activation is the ...Inositol-1,4,5-trisphosphate receptors (InsPRs) are cation channels that mobilize Ca from intracellular stores in response to a wide range of cellular stimuli. The paradigm of InsPR activation is the coupled interplay between binding of InsP and Ca that switches the ion conduction pathway between closed and open states to enable the passage of Ca through the channel. However, the molecular mechanism of how the receptor senses and decodes ligand-binding signals into gating motion remains unknown. Here, we present the electron cryo-microscopy structure of InsPR1 from rat cerebellum determined to 4.1 Å resolution in the presence of activating concentrations of Ca and adenophostin A (AdA), a structural mimetic of InsP and the most potent known agonist of the channel. Comparison with the 3.9 Å-resolution structure of InsPR1 in the Apo-state, also reported herein, reveals the binding arrangement of AdA in the tetrameric channel assembly and striking ligand-induced conformational rearrangements within cytoplasmic domains coupled to the dilation of a hydrophobic constriction at the gate. Together, our results provide critical insights into the mechanistic principles by which ligand-binding allosterically gates InsPR channel.
External linksCell Res / PubMed:30470765 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 4.3 Å
Structure data

9243

6mu1

EMDB-9243: Structure of full-length IP3R1 channel bound with Adenophostin A (composite)
PDB-6mu1: Structure of full-length IP3R1 channel bound with Adenophostin A
Method: EM (single particle) / Resolution: 4.1 Å

9244

6mu2

EMDB-9244: Structure of full-length IP3R1 channel in Apo-state (composite)
PDB-6mu2: Structure of full-length IP3R1 channel in the Apo-state
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-9245:
Structure of full-length IP3R1 channel in ligand-bound state
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-9246:
Structure of full-length IP3R1 channel in Apo-state
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-9247:
Structure of full-length IP3R1 channel in ligand-bound state
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-9248:
Structure of full-length IP3R1 channel in Apo-state
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-JYP:
Adenophostin A

Source
  • rattus norvegicus (Norway rat)
  • Rat (rat)
KeywordsMEMBRANE PROTEIN / inositol 1 / 4 / 5-trisphosphate receptor / calcium release channel / neuronal type 1 / adenophostin A / IP3R-channel activator

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