ジャーナル: J Mol Biol / 年: 2017 タイトル: Spatial Organization and Molecular Interactions of the Schizosaccharomyces pombe Ccq1-Tpz1-Poz1 Shelterin Complex. 著者: Harry Scott / Jin-Kwang Kim / Clinton Yu / Lan Huang / Feng Qiao / Derek J Taylor / 要旨: The shelterin complex is a macromolecular assembly of proteins that binds to and protects telomeric DNA, which composes the ends of all linear chromosomes. Shelterin proteins prevent chromosome ends ...The shelterin complex is a macromolecular assembly of proteins that binds to and protects telomeric DNA, which composes the ends of all linear chromosomes. Shelterin proteins prevent chromosome ends from fusing together and from eliciting erroneous induction of DNA damage response pathways. In addition, shelterin proteins play key roles in regulating the recruitment and activation of telomerase, an enzyme that extends telomeric DNA. In fission yeast, Schizosaccharomyces pombe, interactions between the shelterin proteins Ccq1, Tpz1, and Poz1 are important for regulating telomerase-mediated telomere synthesis and thus telomere length homeostasis. Here, we used electron microscopy combined with genetic labeling to define the three-dimensional arrangement of the S. pombe Ccq1-Tpz1-Poz1 (CTP) complex. Crosslinking mass spectrometry was used to identify individual residues that are in proximity to the protein-protein interfaces of the assembled CTP complex. Together, our data provide a first glimpse into the architectural design of the CTP complex and reveals unique interactions that are important in maintaining the S. pombe telomere in a non-extendible state.
ダウンロード / ファイル: emd_8865.map.gz / 形式: CCP4 / 大きさ: 3.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Negative-stain reconstruction of an N-terminally MBP tagged Poz1 in the S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249)
ボクセルのサイズ
X=Y=Z: 3.004 Å
密度
表面レベル
登録者による: 0.0436 / ムービー #1: 0.0436
最小 - 最大
-0.081371136 - 0.13343428
平均 (標準偏差)
-0.0010259209 (±0.010540782)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
100
100
100
Spacing
100
100
100
セル
A=B=C: 300.4 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
3.004
3.004
3.004
M x/y/z
100
100
100
origin x/y/z
0.000
0.000
0.000
length x/y/z
300.400
300.400
300.400
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
0
0
0
NX/NY/NZ
28
11
56
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
100
100
100
D min/max/mean
-0.081
0.133
-0.001
-
添付データ
-
試料の構成要素
-
全体 : Negative-stain reconstruction of an N-terminally MBP tagged Poz1 ...
全体
名称: Negative-stain reconstruction of an N-terminally MBP tagged Poz1 in the S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249)
要素
複合体: Negative-stain reconstruction of an N-terminally MBP tagged Poz1 in the S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249)
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超分子 #1: Negative-stain reconstruction of an N-terminally MBP tagged Poz1 ...
超分子
名称: Negative-stain reconstruction of an N-terminally MBP tagged Poz1 in the S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249) タイプ: complex / ID: 1 / 親要素: 0 / 詳細: Complex is a dimer of trimers