National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI095366
United States
Citation
Journal: Proc Natl Acad Sci U S A / Year: 2017 Title: Structural studies of Chikungunya virus maturation. Authors: Moh Lan Yap / Thomas Klose / Akane Urakami / S Saif Hasan / Wataru Akahata / Michael G Rossmann / Abstract: Cleavage of the alphavirus precursor glycoprotein p62 into the E2 and E3 glycoproteins before assembly with the nucleocapsid is the key to producing fusion-competent mature spikes on alphaviruses. ...Cleavage of the alphavirus precursor glycoprotein p62 into the E2 and E3 glycoproteins before assembly with the nucleocapsid is the key to producing fusion-competent mature spikes on alphaviruses. Here we present a cryo-EM, 6.8-Å resolution structure of an "immature" Chikungunya virus in which the cleavage site has been mutated to inhibit proteolysis. The spikes in the immature virus have a larger radius and are less compact than in the mature virus. Furthermore, domains B on the E2 glycoproteins have less freedom of movement in the immature virus, keeping the fusion loops protected under domain B. In addition, the nucleocapsid of the immature virus is more compact than in the mature virus, protecting a conserved ribosome-binding site in the capsid protein from exposure. These differences suggest that the posttranslational processing of the spikes and nucleocapsid is necessary to produce infectious virus.
History
Deposition
May 18, 2017
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Header (metadata) release
Aug 23, 2017
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Map release
Dec 6, 2017
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Update
Dec 11, 2019
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Current status
Dec 11, 2019
Processing site: RCSB / Status: Released
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Structure visualization
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Surface view with section colored by density value
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