+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-8145 | |||||||||
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Title | Faustovirus inner capsid shell | |||||||||
Map data | None | |||||||||
Sample |
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Keywords | virus / inner shell / VIRUS LIKE PARTICLE | |||||||||
Biological species | Faustovirus | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 26.4 Å | |||||||||
Authors | Klose T / Rossmann MG | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Proc Natl Acad Sci U S A / Year: 2016 Title: Structure of faustovirus, a large dsDNA virus. Authors: Thomas Klose / Dorine G Reteno / Samia Benamar / Adam Hollerbach / Philippe Colson / Bernard La Scola / Michael G Rossmann / Abstract: Many viruses protect their genome with a combination of a protein shell with or without a membrane layer. Here we describe the structure of faustovirus, the first DNA virus (to our knowledge) that ...Many viruses protect their genome with a combination of a protein shell with or without a membrane layer. Here we describe the structure of faustovirus, the first DNA virus (to our knowledge) that has been found to use two protein shells to encapsidate and protect its genome. The crystal structure of the major capsid protein, in combination with cryo-electron microscopy structures of two different maturation stages of the virus, shows that the outer virus shell is composed of a double jelly-roll protein that can be found in many double-stranded DNA viruses. The structure of the repeating hexameric unit of the inner shell is different from all other known capsid proteins. In addition to the unique architecture, the region of the genome that encodes the major capsid protein stretches over 17,000 bp and contains a large number of introns and exons. This complexity might help the virus to rapidly adapt to new environments or hosts. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_8145.map.gz | 61.8 MB | EMDB map data format | |
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Header (meta data) | emd-8145-v30.xml emd-8145.xml | 9.2 KB 9.2 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_8145_fsc.xml | 14.6 KB | Display | FSC data file |
Images | emd_8145.png | 275.1 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-8145 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-8145 | HTTPS FTP |
-Validation report
Summary document | emd_8145_validation.pdf.gz | 717.4 KB | Display | EMDB validaton report |
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Full document | emd_8145_full_validation.pdf.gz | 716.9 KB | Display | |
Data in XML | emd_8145_validation.xml.gz | 13.2 KB | Display | |
Data in CIF | emd_8145_validation.cif.gz | 17.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-8145 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-8145 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_8145.map.gz / Format: CCP4 / Size: 163.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | None | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 7.58 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Faustovirus
Entire | Name: Faustovirus |
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Components |
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-Supramolecule #1: Faustovirus
Supramolecule | Name: Faustovirus / type: virus / ID: 1 / Parent: 0 Details: Virus was isolated from Vermamoeba vermiformis and the inner shell was purified using density centrifugation. NCBI-ID: 1477405 / Sci species name: Faustovirus / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No |
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Host (natural) | Organism: Vermamoeba vermiformis (eukaryote) / Strain: CDC 19 |
Virus shell | Shell ID: 1 / Name: inner capsid shell / Diameter: 1900.0 Å / T number (triangulation number): 16 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Average electron dose: 25.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |