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- EMDB-7950: cryo-EM density map of ClpP from Staphylococcus aureus (Wild Type) -
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Open data
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Basic information
Entry | Database: EMDB / ID: 7950 |
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Title | cryo-EM density map of ClpP from Staphylococcus aureus (Wild Type) |
![]() | ClpP from Staphylococcus aureus |
![]() | Caseinolytic protease from Staphylococcus aureus![]() |
Source | ![]() ![]() |
Method | ![]() ![]() |
![]() | Ripstein ZA / Vahidi S / Kay LE / Rubinstein JL |
![]() | Journal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2018 Title: Reversible inhibition of the ClpP protease via an N-terminal conformational switch. ![]() |
Date | Deposition: May 29, 2018 / Header (metadata) release: Jun 27, 2018 / Map release: Jun 27, 2018 / Last update: Jul 25, 2018 |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
![]() | ![]() |
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Header (meta data in XML format) | ![]() ![]() ![]() |
Images | ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-7950 |
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Links
-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.45 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire Caseinolytic protease from Staphylococcus aureus
Entire | Name: Caseinolytic protease from Staphylococcus aureus / Number of components: 2 |
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-Component #1: protein, Caseinolytic protease from Staphylococcus aureus
Protein | Name: Caseinolytic protease from Staphylococcus aureus![]() Recombinant expression: No |
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Mass | Experimental: 301 kDa |
Source | Species: ![]() ![]() |
Source (engineered) | Expression System: ![]() ![]() ![]() |
-Component #2: protein, Caseinolytic protease from Staphylococcus aureus
Protein | Name: Caseinolytic protease from Staphylococcus aureus![]() Recombinant expression: No |
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Source | Species: ![]() ![]() |
Source (engineered) | Expression System: ![]() ![]() ![]() |
-Experimental details
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Sample preparation
Specimen | Specimen state: particle / Method: ![]() |
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Sample solution | Specimen conc.: 30 mg/ml / pH: 7 |
Vitrification | Instrument: FEI VITROBOT MARK III / Cryogen name: OTHER / Temperature: 277 K / Humidity: 100 % / Details: Modified Vitrobot. |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |
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![]() | Microscope: FEI TECNAI F20 |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Magnification: 25000. X (nominal) / Cs: 2 mm / Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: GATAN LIQUID NITROGEN |
Camera | Detector: GATAN K2 SUMMIT (4k x 4k) |
-Image acquisition
Image acquisition | Number of digital images: 104 |
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Image processing
![]() | Method: ![]() |
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3D reconstruction | Algorithm: FOURIER SPACE / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF |