- EMDB-7858: Ectodomain of full length, wild type HIV-1 glycoprotein clone PC6... -
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Database: EMDB / ID: 7858
Title
Ectodomain of full length, wild type HIV-1 glycoprotein clone PC64M18C043 in complex with PGT151 Fab
Map data
Ectodomain of full length, wild type HIV-1 glycoprotein clone PC64M18C043 in complex with PGT151 Fab
Sample
Full length, wild type HIV-1 Envelope glycoprotein in complex with PGT151 Fab: (Envelope glycoprotein ...) x 2 / (Immunoglobulin G PGT151 Fab, ...) x 2 / (ligand) x 5
Journal: Cell Rep / Year: 2018 Title: Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design. Authors: Kimmo Rantalainen / Zachary T Berndsen / Sasha Murrell / Liwei Cao / Oluwarotimi Omorodion / Jonathan L Torres / Mengyu Wu / Jeffrey Umotoy / Jeffrey Copps / Pascal Poignard / Elise Landais / James C Paulson / Ian A Wilson / Andrew B Ward Abstract: Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. Longitudinal analyses of both neutralizing antibody lineages and viruses at ...Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system. Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort. Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes. Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers. These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.
Entire Full length, wild type HIV-1 Envelope glycoprotein in complex wit...
Entire
Name: Full length, wild type HIV-1 Envelope glycoprotein in complex with PGT151 Fab Details: Full length HIV-1 Envelope glycoprotein clone C043 collected 18 months post infection from donor 64 of Protocol C cohort study. Expressed as recombinant protein in HEK293F cell line. Number of components: 10
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Component #1: protein, Full length, wild type HIV-1 Envelope glycoprotein in co...
Protein
Name: Full length, wild type HIV-1 Envelope glycoprotein in complex with PGT151 Fab Details: Full length HIV-1 Envelope glycoprotein clone C043 collected 18 months post infection from donor 64 of Protocol C cohort study. Expressed as recombinant protein in HEK293F cell line. Recombinant expression: No
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