ジャーナル: Structure / 年: 2018 タイトル: N-Terminal Domains of Cardiac Myosin Binding Protein C Cooperatively Activate the Thin Filament. 著者: Cristina Risi / Betty Belknap / Eva Forgacs-Lonart / Samantha P Harris / Gunnar F Schröder / Howard D White / Vitold E Galkin / 要旨: Muscle contraction relies on interaction between myosin-based thick filaments and actin-based thin filaments. Myosin binding protein C (MyBP-C) is a key regulator of actomyosin interactions. Recent ...Muscle contraction relies on interaction between myosin-based thick filaments and actin-based thin filaments. Myosin binding protein C (MyBP-C) is a key regulator of actomyosin interactions. Recent studies established that the N'-terminal domains (NTDs) of MyBP-C can either activate or inhibit thin filaments, but the mechanism of their collective action is poorly understood. Cardiac MyBP-C (cMyBP-C) harbors an extra NTD, which is absent in skeletal isoforms of MyBP-C, and its role in regulation of cardiac contraction is unknown. Here we show that the first two domains of human cMyPB-C (i.e., C0 and C1) cooperate to activate the thin filament. We demonstrate that C1 interacts with tropomyosin via a positively charged loop and that this interaction, stabilized by the C0 domain, is required for thin filament activation by cMyBP-C. Our data reveal a mechanism by which cMyBP-C can modulate cardiac contraction and demonstrate a function of the C0 domain.
全体 : cardiac thin filament decorated with C0C1 fragment of cardiac myo...
全体
名称: cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1
要素
細胞器官・細胞要素: cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1
複合体: actin
タンパク質・ペプチド: Actin, cytoplasmic 2
複合体: myosin binding protein C
タンパク質・ペプチド: Myosin-binding protein C, cardiac-type
タンパク質・ペプチド: Myosin-binding protein C, cardiac-type
複合体: tropomyosin
タンパク質・ペプチド: Tropomyosin
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超分子 #1: cardiac thin filament decorated with C0C1 fragment of cardiac myo...
超分子
名称: cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1 タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 / 含まれる分子: all