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- EMDB-7096: Cryo-EM structure of the TMEM16A calcium-activated chloride chann... -

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Basic information

Database: EMDB / ID: 7096
TitleCryo-EM structure of the TMEM16A calcium-activated chloride channel in LMNG
SampleTMEM16A Calcium-activated chloride channel
SourceMus musculus / mammal / ハツカネズミ, はつかねずみ /
Map datasharpened map
Methodsingle particle reconstruction, at 3.8 Å resolution
AuthorsDang S / Feng S
CitationNature, 2017, 552, 426-429

Nature, 2017, 552, 426-429 Yorodumi Papers
Cryo-EM structures of the TMEM16A calcium-activated chloride channel.
Shangyu Dang / Shengjie Feng / Jason Tien / Christian J Peters / David Bulkley / Marco Lolicato / Jianhua Zhao / Kathrin Zuberbühler / Wenlei Ye / Lijun Qi / Tingxu Chen / Charles S Craik / Yuh Nung Jan / Daniel L Minor / Yifan Cheng / Lily Yeh Jan

Validation ReportPDB-ID: 6bgj

SummaryFull reportAbout validation report
DateDeposition: Oct 28, 2017 / Header (metadata) release: Dec 20, 2017 / Map release: Dec 27, 2017 / Last update: Jan 17, 2018

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 5.5
  • Imaged by UCSF CHIMERA
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  • Surface view colored by cylindrical radius
  • Surface level: 5.5
  • Imaged by UCSF CHIMERA
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  • Surface view with fitted model
  • Atomic models: : PDB-6bgj
  • Surface level: 5.5
  • Imaged by UCSF CHIMERA
  • Download
3D viewer

View / / Stereo:
Slabnear <=> far

fix: /
Orientation Rotation
Misc. /
Supplemental images

Downloads & links


Fileemd_7096.map.gz (map file in CCP4 format, 67109 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
256 pix
1.02 Å/pix.
= 261.12 Å
256 pix
1.02 Å/pix.
= 261.12 Å
256 pix
1.02 Å/pix.
= 261.12 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 1.02 Å
Contour Level:5.5 (by author), 5.5 (movie #1):
Minimum - Maximum-25.658325 - 36.385464
Average (Standard dev.)-3.603186E-9 (1)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 261.12 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.021.021.02
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z261.120261.120261.120
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
D min/max/mean-25.65836.385-0.000

Supplemental data

Sample components

Entire TMEM16A Calcium-activated chloride channel

EntireName: TMEM16A Calcium-activated chloride channel / Number of components: 3

Component #1: cellular-component, TMEM16A Calcium-activated chloride channel

Cellular-componentName: TMEM16A Calcium-activated chloride channel / Recombinant expression: No
SourceSpecies: Mus musculus / mammal / ハツカネズミ, はつかねずみ /

Component #2: protein, Anoctamin-1

ProteinName: Anoctamin-1 / Details: Calcium ions / Recombinant expression: No
MassTheoretical: 105.603984 kDa
Source (engineered)Expression System: Mus musculus / mammal / ハツカネズミ, はつかねずみ /

Component #3: ligand, CALCIUM ION

LigandName: CALCIUM ION / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 4.007805 MDa

Experimental details

Sample preparation

Specimen stateparticle
Sample solutionSpecimen conc.: 0.5 mg/ml / pH: 9
VitrificationInstrument: FEI VITROBOT MARK III / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 % / Details: blot 6-8 seconds before plunging

Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 80 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 29000 X (calibrated) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD
CameraDetector: GATAN K2 (4k x 4k)

Image acquisition

Image acquisitionNumber of digital images: 7882
Details: 5326 images for TMEM16A alone in LMNG, 2556 images for TMEM16A with Fab bound in LMNG

Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C2 (2 fold cyclic) / Number of projections: 251851
3D reconstructionAlgorithm: BACK PROJECTION / Software: RELION / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF

Atomic model buiding

Output model

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