ジャーナル: Cell / 年: 2016 タイトル: Structure of Mammalian Respiratory Supercomplex IIIIIV. 著者: Meng Wu / Jinke Gu / Runyu Guo / Yushen Huang / Maojun Yang / 要旨: The mammalian respiratory chain complexes assemble into supercomplexes (SCs) and reside in the inner mitochondrial membrane to transfer electrons and establish the proton gradient for complex V to ...The mammalian respiratory chain complexes assemble into supercomplexes (SCs) and reside in the inner mitochondrial membrane to transfer electrons and establish the proton gradient for complex V to synthesize ATP. The precise arrangement of SCs is largely unknown. Here, we report a 4.0-Å cryo-electron microscopy (cryo-EM) structure of the major SC in porcine heart, the 1.7-MDa SCIIIIIV. The complex III (CIII) dimer and complex IV (CIV) bind at the same side of the L-shaped complex I (CI). Several accessory or supernumerary subunits of CI, such as NDUFA11, NDUFB4, NDUFB8, and NDUFB9, directly contribute to the oligomerization of CI, CIII, and CIV. COX7C and COX7A of CIV attach CIV to the concave surface formed by CIII and the distal end of membrane arm of CI. The structure suggests a possible mechanism by which electrons are transferred from NADH to cytochrome c and provides a platform for future functional dissection of respiration.
ダウンロード / ファイル: emd_6718.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
This map was obtained by sub-region refinement.
ボクセルのサイズ
X=Y=Z: 1.08 Å
密度
表面レベル
登録者による: 0.0468 / ムービー #1: 0.0468
最小 - 最大
-0.14976624 - 0.30246428
平均 (標準偏差)
0.00039575636 (±0.0052151834)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
480
480
480
Spacing
480
480
480
セル
A=B=C: 518.4 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.08
1.08
1.08
M x/y/z
480
480
480
origin x/y/z
0.000
0.000
0.000
length x/y/z
518.400
518.400
518.400
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
480
480
480
D min/max/mean
-0.150
0.302
0.000
-
添付データ
-
試料の構成要素
-
全体 : Cryo-EM structure of mammalian respiratory complex III2IV1 by sub...
全体
名称: Cryo-EM structure of mammalian respiratory complex III2IV1 by subregion refinement
要素
複合体: Cryo-EM structure of mammalian respiratory complex III2IV1 by subregion refinement
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超分子 #1: Cryo-EM structure of mammalian respiratory complex III2IV1 by sub...
超分子
名称: Cryo-EM structure of mammalian respiratory complex III2IV1 by subregion refinement タイプ: complex / ID: 1 / 親要素: 0 詳細: The map of complex III2IV1 was obtained by subregion refinement of Supercomplex I1III2IV1
由来(天然)
生物種: Sus scrofa (ブタ)
分子量
実験値: 700 KDa
-
実験情報
-
構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
-
試料調製
濃度
0.2 mg/mL
緩衝液
pH: 7.4
凍結
凍結剤: ETHANE / チャンバー内湿度: 100 %
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電子顕微鏡法
顕微鏡
FEI TITAN KRIOS
撮影
フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 1.25 e/Å2