ジャーナル: Nat Struct Mol Biol / 年: 2016 タイトル: Cryo-electron microscopy structure of the TRPV2 ion channel. 著者: Lejla Zubcevic / Mark A Herzik / Ben C Chung / Zhirui Liu / Gabriel C Lander / Seok-Yong Lee / 要旨: Transient receptor potential vanilloid (TRPV) cation channels are polymodal sensors involved in a variety of physiological processes. TRPV2, a member of the TRPV family, is regulated by temperature, ...Transient receptor potential vanilloid (TRPV) cation channels are polymodal sensors involved in a variety of physiological processes. TRPV2, a member of the TRPV family, is regulated by temperature, by ligands, such as probenecid and cannabinoids, and by lipids. TRPV2 has been implicated in many biological functions, including somatosensation, osmosensation and innate immunity. Here we present the atomic model of rabbit TRPV2 in its putative desensitized state, as determined by cryo-EM at a nominal resolution of ∼4 Å. In the TRPV2 structure, the transmembrane segment 6 (S6), which is involved in gate opening, adopts a conformation different from the one observed in TRPV1. Structural comparisons of TRPV1 and TRPV2 indicate that a rotation of the ankyrin-repeat domain is coupled to pore opening via the TRP domain, and this pore opening can be modulated by rearrangements in the secondary structure of S6.
Pre-processing was performed in the Appion pipeline using a template for particle selection, CTFFIND3 for CTF estimation, MSA/MRA for 2D classification, and RELION for 3D classification and refinement.
CTF補正
詳細: whole micrograph
最終 再構成
アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 3.8 Å / 解像度の算出法: OTHER ソフトウェア - 名称: Appion, CTFFIND3, FindEM, EMAN, RELION 詳細: Per-frame dose weighting was performed using the RELION particle polishing method. 使用した粒子像数: 43879