+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-6411 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of PhnGHIJ complex from Escherichia coli | |||||||||
Map data | PhnGHIJ complex from Escherichia coli | |||||||||
Sample |
| |||||||||
Keywords | PhnGHIJ | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 7.8 Å | |||||||||
Authors | Yang K / Ren Z / Raushel FM / Zhang J | |||||||||
Citation | Journal: Structure / Year: 2016 Title: Structures of the Carbon-Phosphorus Lyase Complex Reveal the Binding Mode of the NBD-like PhnK. Authors: Kailu Yang / Zhongjie Ren / Frank M Raushel / Junjie Zhang / Abstract: The carbon-phosphorus (C-P) lyase complex is essential for the metabolism of unactivated phosphonates to phosphate in bacteria. Using single-particle cryo-electron microscopy, we determined two ...The carbon-phosphorus (C-P) lyase complex is essential for the metabolism of unactivated phosphonates to phosphate in bacteria. Using single-particle cryo-electron microscopy, we determined two structures of the C-P lyase core complex PhnG2H2I2J2, with or without PhnK. PhnG2H2I2J2 is a two-fold symmetric hetero-octamer. Its two PhnJ subunits provide two identical binding sites for PhnK. Only one PhnK binds to PhnG2H2I2J2 due to steric hindrance. PhnK is homologous to the nucleotide-binding domain (NBD) of ATP-binding cassette transporters. The α helices 3 and 4 of PhnK bind to α helix 6 and a loop (residues 227-230) of PhnJ, in a different mode from the binding of NBDs to their transmembrane partners. Moreover, binding of PhnK exposes the active site residue, Gly32 of PhnJ, located near the interface between PhnJ and PhnH. This structural information provides a basis for further deciphering of the reaction mechanism of the C-P lyase. | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_6411.map.gz | 153.4 KB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-6411-v30.xml emd-6411.xml | 17.2 KB 17.2 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_6411_fsc.xml | 2.2 KB | Display | FSC data file |
Images | emd_6411.png | 68.7 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-6411 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6411 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|
-Map
File | Download / File: emd_6411.map.gz / Format: CCP4 / Size: 825.2 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | PhnGHIJ complex from Escherichia coli | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Sample components
-Entire : PhnGHIJ complex from Escherichia coli
Entire | Name: PhnGHIJ complex from Escherichia coli |
---|---|
Components |
|
-Supramolecule #1000: PhnGHIJ complex from Escherichia coli
Supramolecule | Name: PhnGHIJ complex from Escherichia coli / type: sample / ID: 1000 / Oligomeric state: Two PhnG, two PhnH, two PhnI, two PhnJ / Number unique components: 4 |
---|---|
Molecular weight | Theoretical: 220 KDa |
-Macromolecule #1: PhnG
Macromolecule | Name: PhnG / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: Escherichia coli (E. coli) / Strain: BW5328 |
Molecular weight | Theoretical: 18.7 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) / Recombinant strain: Rosetta2 (DE3) pLysS cells (Novagen) / Recombinant plasmid: pET-28b |
-Macromolecule #2: PhnH
Macromolecule | Name: PhnH / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: Escherichia coli (E. coli) / Strain: BW5328 |
Molecular weight | Theoretical: 20.9 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) / Recombinant strain: Rosetta2 (DE3) pLysS cells (Novagen) / Recombinant plasmid: pET-28b |
-Macromolecule #3: PhnI
Macromolecule | Name: PhnI / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: Escherichia coli (E. coli) / Strain: BW5328 |
Molecular weight | Theoretical: 38.9 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) / Recombinant strain: Rosetta2 (DE3) pLysS cells (Novagen) / Recombinant plasmid: pET-28b |
-Macromolecule #4: PhnJ
Macromolecule | Name: PhnJ / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: Escherichia coli (E. coli) / Strain: BW5328 |
Molecular weight | Theoretical: 31.7 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) / Recombinant strain: Rosetta2 (DE3) pLysS cells (Novagen) / Recombinant plasmid: pET-28b |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.1 mg/mL |
---|---|
Buffer | pH: 8.5 / Details: 50 mM HEPES, 150 mM NaCl, 2 mM TCEP |
Grid | Details: C-Flat 200 mesh 1.2/1.3 holey carbon grid |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 91 K / Instrument: FEI VITROBOT MARK III / Method: Blot for 5 seconds before plunging |
-Electron microscopy #1
Microscope | FEI TECNAI F20 |
---|---|
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Calibrated magnification: 33333 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 25000 |
Sample stage | Specimen holder: Nitrogen-cooled / Specimen holder model: SIDE ENTRY, EUCENTRIC |
Temperature | Min: 77 K / Max: 105 K / Average: 100 K |
Microscopy ID | 1 |
Details | Counting mode with a dose rate of 10 e/(pixel^2*s), 0.2 s/frame |
Date | Nov 14, 2014 |
Image recording | Category: CCD / Film or detector model: GATAN K2 (4k x 4k) / Number real images: 967 / Average electron dose: 22 e/Å2 Details: Every image is the average of twenty-five or fifty frames recorded by the direct electron detector. |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |
-Electron microscopy #2
Microscope | FEI TECNAI F20 |
---|---|
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Calibrated magnification: 33333 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 25000 |
Sample stage | Specimen holder: Nitrogen-cooled / Specimen holder model: SIDE ENTRY, EUCENTRIC |
Temperature | Min: 77 K / Max: 105 K / Average: 100 K |
Microscopy ID | 2 |
Details | Counting mode with a dose rate of 10 e/(pixel^2*s), 0.2 s/frame |
Date | Nov 29, 2014 |
Image recording | Category: CCD / Film or detector model: GATAN K2 (4k x 4k) / Number real images: 967 / Average electron dose: 44 e/Å2 Details: Every image is the average of twenty-five or fifty frames recorded by the direct electron detector. |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |
-Electron microscopy #3
Microscope | FEI TECNAI F20 |
---|---|
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Calibrated magnification: 33333 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 25000 |
Sample stage | Specimen holder: Nitrogen-cooled / Specimen holder model: SIDE ENTRY, EUCENTRIC |
Temperature | Min: 77 K / Max: 105 K / Average: 100 K |
Microscopy ID | 3 |
Details | Counting mode with a dose rate of 10 e/(pixel^2*s), 0.2 s/frame |
Date | Dec 14, 2014 |
Image recording | Category: CCD / Film or detector model: GATAN K2 (4k x 4k) / Number real images: 967 / Average electron dose: 44 e/Å2 Details: Every image is the average of twenty-five or fifty frames recorded by the direct electron detector. |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |
-Image processing
-Atomic model buiding 1
Initial model | PDB ID: Chain - Chain ID: A |
---|---|
Software | Name: Chimera |
Refinement | Space: REAL / Protocol: RIGID BODY FIT |