|Entry||Database: EMDB / ID: 6338|
|Title||Cryo-EM study of the Eukaryotic Minichromosome Maintenance Complex|
|Map data||Reconstruction of the Eukaryotic Minichromosome Maintenance Complex|
|Sample||Eukaryotic Minichromosome Maintenance Complex (MCM2-7)|
|Keywords||Cryo-EM / single particle / MCM2-7 / DNA replication|
|Function / homology||P-loop containing nucleoside triphosphate hydrolase / Mini-chromosome maintenance protein / MCM domain / MCM OB domain / AAA+ ATPase domain / DNA replication licensing factor Mcm2 / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm5 / DNA replication licensing factor Mcm6 ...P-loop containing nucleoside triphosphate hydrolase / Mini-chromosome maintenance protein / MCM domain / MCM OB domain / AAA+ ATPase domain / DNA replication licensing factor Mcm2 / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm5 / DNA replication licensing factor Mcm6 / MCM P-loop domain / DNA replication licensing factor Mcm7 / Mini-chromosome maintenance protein 2 / MCM N-terminal domain / MCM OB domain / MCM family signature. / MCM family domain profile. / Nucleic acid-binding, OB-fold / Orc1 removal from chromatin / Mini-chromosome maintenance, conserved site / Activation of the pre-replicative complex / Switching of origins to a post-replicative state / MCM N-terminal domain / MCM complex binding / negative regulation of ATP-dependent DNA helicase activity / MCM core complex / CMG complex / nuclear DNA replication / negative regulation of chromatin silencing at telomere / MCM complex / establishment of chromatin silencing / mitotic DNA replication initiation / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / regulation of DNA-dependent DNA replication initiation / nuclear pre-replicative complex / DNA replication preinitiation complex / single-stranded DNA-dependent ATPase activity / double-strand break repair via break-induced replication / nuclear replication fork / DNA strand elongation involved in DNA replication / replication fork protection complex / chromatin silencing at silent mating-type cassette / DNA duplex unwinding / DNA helicase activity / DNA unwinding involved in DNA replication / chromatin silencing at telomere / DNA replication origin binding / DNA replication initiation / DNA helicase / helicase activity / chromosome, telomeric region / single-stranded DNA binding / nuclear chromosome, telomeric region / cellular response to DNA damage stimulus / chromatin binding / nucleoplasm / ATP binding / nucleus / metal ion binding / cytoplasm / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM2 / Minichromosome maintenance protein 5 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM6|
Function and homology information
|Source||Saccharomyces cerevisiae S288c (Yeast)|
|Method||single particle reconstruction / cryo EM / 3.8 Å resolution|
|Authors||Li N / Zhai Y / Zhang Y / Li W / Yang M / Lei J / Tye BK / Gao N|
|Citation||Journal: Nature / Year: 2015|
Title: Structure of the eukaryotic MCM complex at 3.8 Å.
Authors: Ningning Li / Yuanliang Zhai / Yixiao Zhang / Wanqiu Li / Maojun Yang / Jianlin Lei / Bik-Kwoon Tye / Ning Gao
|Validation Report||PDB-ID: 3ja8|
About validation report
|Date||Deposition: May 9, 2015 / Header (metadata) release: Jul 15, 2015 / Map release: Aug 5, 2015 / Last update: Aug 19, 2015|
|Structure viewer||EM map: |
Downloads & links
|File||emd_6338.map.gz (map file in CCP4 format, 105470 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.32 Å|
CCP4 map header:
-Entire Eukaryotic Minichromosome Maintenance Complex (MCM2-7)
|Entire||Name: Eukaryotic Minichromosome Maintenance Complex (MCM2-7)|
Number of components: 1 / Oligomeric State: Double hexamer
|Mass||Theoretical: 1.2 MDa|
-Component #1: protein, Eukaryotic Minichromosome Maintenance Complex
|Protein||Name: Eukaryotic Minichromosome Maintenance Complex / Oligomeric Details: hexamer / Recombinant expression: No / Number of Copies: 2|
|Source||Species: Saccharomyces cerevisiae S288c (Yeast)|
|Source (natural)||Location in cell: cytoplasm|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||Buffer solution: 50mM HEPES-KOH, 100mM K glutamate, 10mM Mg(OAc)2, 0.25% Triton X-100, 3mM ATP, 1mM EDTA|
|Vitrification||Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 %|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS / Date: Nov 25, 2015|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 16 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 22500 X (nominal) / Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: FEI TITAN KRIOS AUTOGRID HOLDER|
|Camera||Detector: GATAN K2 (4k x 4k)|
|Image acquisition||Number of digital images: 2230 / Sampling size: 4 microns|
Details: Every image is the average of 14 frames recorded by the direct electron detector
|Processing||Method: single particle reconstruction / Applied symmetry: C2 (2 fold cyclic) / Number of projections: 85365|
|3D reconstruction||Software: RELION / CTF correction: CTFFIND / Resolution: 3.8 Å / Resolution method: FSC 0.143, gold-standard|
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