- EMDB-5234: transfer-messenger RNA in complex with the ribosome in the resume... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-5234
タイトル
transfer-messenger RNA in complex with the ribosome in the resume state in E.coli cells
マップデータ
This is the map of the tmRNA-ribosome complex in the resume state
試料
試料: transfer-messenger RNA bound to ribosome complex
複合体: ribosome
RNA: transfer-messenger RNA
キーワード
transfer-messenger RNA / trans-translation / small protein B
機能・相同性
trans-translation / SsrA-binding protein / SsrA-binding protein, conserved site / Small protein B / SmpB protein / SsrA-binding protein. / rRNA binding / cytosol / SsrA-binding protein
ジャーナル: EMBO J / 年: 2010 タイトル: Visualizing the transfer-messenger RNA as the ribosome resumes translation. 著者: Jie Fu / Yaser Hashem / Iwona Wower / Jianlin Lei / Hstau Y Liao / Christian Zwieb / Jacek Wower / Joachim Frank / 要旨: Bacterial ribosomes stalled by truncated mRNAs are rescued by transfer-messenger RNA (tmRNA), a dual-function molecule that contains a tRNA-like domain (TLD) and an internal open reading frame (ORF). ...Bacterial ribosomes stalled by truncated mRNAs are rescued by transfer-messenger RNA (tmRNA), a dual-function molecule that contains a tRNA-like domain (TLD) and an internal open reading frame (ORF). Occupying the empty A site with its TLD, the tmRNA enters the ribosome with the help of elongation factor Tu and a protein factor called small protein B (SmpB), and switches the translation to its own ORF. In this study, using cryo-electron microscopy, we obtained the first structure of an in vivo-formed complex containing ribosome and the tmRNA at the point where the TLD is accommodated into the ribosomal P site. We show that tmRNA maintains a stable 'arc and fork' structure on the ribosome when its TLD moves to the ribosomal P site and translation resumes on its ORF. Based on the density map, we built an atomic model, which suggests that SmpB interacts with the five nucleotides immediately upstream of the resume codon, thereby determining the correct selection of the reading frame on the ORF of tmRNA.