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- EMDB-0101: T. thermophilus hibernating 70S ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-0101
TitleT. thermophilus hibernating 70S ribosome
Map dataCryo-EM reconstruction of Thermus thermophilus 70S ribosome from hibernating 100S ribosome.
Sample
  • Complex: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer
    • Complex: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer
      • Protein or peptide: x 49 types
      • RNA: x 3 types
    • Complex: Ribosome hibernation promoting factor
      • Protein or peptide: x 1 types
Function / homology
Function and homology information


primary metabolic process / regulation of translation / large ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / transferase activity / tRNA binding / ribosome / rRNA binding ...primary metabolic process / regulation of translation / large ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / transferase activity / tRNA binding / ribosome / rRNA binding / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein ...Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S6, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein S16 domain superfamily / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein
Similarity search - Domain/homology
Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 ...Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bTHX / Ribosome hibernation promoting factor / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL17
Similarity search - Component
Biological speciesThermus thermophilus HB8 (bacteria) / Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.28 Å
AuthorsFlygaard RK / Jenner LB
CitationJournal: Nat Commun / Year: 2018
Title: Cryo-EM structure of the hibernating Thermus thermophilus 100S ribosome reveals a protein-mediated dimerization mechanism.
Authors: Rasmus Kock Flygaard / Niels Boegholm / Marat Yusupov / Lasse B Jenner /
Abstract: In response to cellular stresses bacteria conserve energy by dimerization of ribosomes into inactive hibernating 100S ribosome particles. Ribosome dimerization in Thermus thermophilus is facilitated ...In response to cellular stresses bacteria conserve energy by dimerization of ribosomes into inactive hibernating 100S ribosome particles. Ribosome dimerization in Thermus thermophilus is facilitated by hibernation-promoting factor (TtHPF). In this study we demonstrate high sensitivity of Tt100S formation to the levels of TtHPF and show that a 1:1 ratio leads to optimal dimerization. We report structures of the T. thermophilus 100S ribosome determined by cryo-electron microscopy to average resolutions of 4.13 Å and 4.57 Å. In addition, we present a 3.28 Å high-resolution cryo-EM reconstruction of a 70S ribosome from a hibernating ribosome dimer and reveal a role for the linker region connecting the TtHPF N- and C-terminal domains in translation inhibition by preventing Shine-Dalgarno duplex formation. Our work demonstrates that species-specific differences in the dimerization interface govern the overall conformation of the 100S ribosome particle and that for Thermus thermophilus no ribosome-ribosome interactions are involved in the interface.
History
DepositionJul 4, 2018-
Header (metadata) releaseAug 22, 2018-
Map releaseOct 24, 2018-
UpdateOct 24, 2018-
Current statusOct 24, 2018Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 3.5
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 3.5
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6gzq
  • Surface level: 3.5
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_0101.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM reconstruction of Thermus thermophilus 70S ribosome from hibernating 100S ribosome.
Voxel sizeX=Y=Z: 1.1 Å
Density
Contour LevelBy AUTHOR: 3.5 / Movie #1: 3.5
Minimum - Maximum-15.041558999999999 - 35.207030000000003
Average (Standard dev.)0.01630383 (±0.43875673)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions700700700
Spacing700700700
CellA=B=C: 770.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.11.11.1
M x/y/z700700700
origin x/y/z0.0000.0000.000
length x/y/z770.000770.000770.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS700700700
D min/max/mean-15.04235.2070.016

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Supplemental data

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Sample components

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Entire : Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer

EntireName: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer
Components
  • Complex: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer
    • Complex: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer
      • Protein or peptide: 50S ribosomal protein L2
      • Protein or peptide: 50S ribosomal protein L3
      • Protein or peptide: 50S ribosomal protein L4
      • Protein or peptide: 50S ribosomal protein L5
      • Protein or peptide: 50S ribosomal protein L6
      • Protein or peptide: 50S ribosomal protein L9
      • Protein or peptide: 50S ribosomal protein L13
      • Protein or peptide: 50S ribosomal protein L14
      • Protein or peptide: 50S ribosomal protein L15
      • Protein or peptide: 50S ribosomal protein L16
      • Protein or peptide: 50S ribosomal protein L17
      • Protein or peptide: 50S ribosomal protein L18
      • Protein or peptide: 50S ribosomal protein L19
      • Protein or peptide: 50S ribosomal protein L20
      • Protein or peptide: 50S ribosomal protein L21
      • Protein or peptide: 50S ribosomal protein L22
      • Protein or peptide: 50S ribosomal protein L23
      • Protein or peptide: 50S ribosomal protein L24
      • Protein or peptide: 50S ribosomal protein L25
      • Protein or peptide: 50S ribosomal protein L27
      • Protein or peptide: 50S ribosomal protein L28
      • Protein or peptide: 50S ribosomal protein L29
      • Protein or peptide: 50S ribosomal protein L30
      • Protein or peptide: 50S ribosomal protein L31
      • Protein or peptide: 50S ribosomal protein L32
      • Protein or peptide: 50S ribosomal protein L33
      • Protein or peptide: 50S ribosomal protein L34
      • Protein or peptide: 50S ribosomal protein L35
      • RNA: 23S ribosomal RNA
      • RNA: 5S ribosomal RNA
      • Protein or peptide: 50S ribosomal protein L36
      • Protein or peptide: 30S ribosomal protein S2
      • Protein or peptide: 30S ribosomal protein S3
      • Protein or peptide: 30S ribosomal protein S4
      • Protein or peptide: 30S ribosomal protein S5
      • Protein or peptide: 30S ribosomal protein S6
      • Protein or peptide: 30S ribosomal protein S7
      • Protein or peptide: 30S ribosomal protein S8
      • Protein or peptide: 30S ribosomal protein S9
      • Protein or peptide: 30S ribosomal protein S10
      • Protein or peptide: 30S ribosomal protein S11
      • Protein or peptide: 30S ribosomal protein S12
      • Protein or peptide: 30S ribosomal protein S13
      • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
      • Protein or peptide: 30S ribosomal protein S15
      • Protein or peptide: 30S ribosomal protein S16
      • Protein or peptide: 30S ribosomal protein S17
      • Protein or peptide: 30S ribosomal protein S18
      • Protein or peptide: 30S ribosomal protein S19
      • Protein or peptide: 30S ribosomal protein S20
      • Protein or peptide: 30S ribosomal protein ThxRibosome
      • RNA: 16S ribosomal RNA
    • Complex: Ribosome hibernation promoting factor
      • Protein or peptide: Ribosome hibernation promoting factor

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Supramolecule #1: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer

SupramoleculeName: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
Molecular weightTheoretical: 2.35 MDa

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Supramolecule #2: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer

SupramoleculeName: Thermus thermophilus 70S hibernating ribosome from 100S ribosome dimer
type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#51, #53
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)

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Supramolecule #3: Ribosome hibernation promoting factor

SupramoleculeName: Ribosome hibernation promoting factor / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #52
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #1: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 29.985799 KDa
SequenceString: AVKKFKPYTP SRRFMTVADF SEITKTEPEK SLVKPLKKTG GRNNQGRITV RFRGGGHKRL YRIIDFKRWD KVGIPAKVAA IEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG T SAQIQGRE ...String:
AVKKFKPYTP SRRFMTVADF SEITKTEPEK SLVKPLKKTG GRNNQGRITV RFRGGGHKRL YRIIDFKRWD KVGIPAKVAA IEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG T SAQIQGRE GDYVILRLPS GELRKVHGEC YATVGAVGNA DHKNIVLGKA GRSRWLGRRP HVRGAAMNPV DHPHGGGEGR AP RGRPPAS PWGWQTKGLK TRKRRKPSSR FIIAR

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Macromolecule #2: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 22.321031 KDa
SequenceString: MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM ...String:
MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM NLEVVDVIPE ENLLLVKGAV PGPNGGLVIV RETKKAA

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Macromolecule #3: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 23.071721 KDa
SequenceString: MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR ...String:
MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR NLPWVVTLAP EGLNVYDIVR TERLVMDLDA WEVFQNRIGG

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Macromolecule #4: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 20.9304 KDa
SequenceString:
PLDVALKRKY YEEVRPELIR RFGYQNVWEV PRLEKVVINQ GLGEAKEDAR ILEKAAQELA LITGQKPAVT RAKKSISNFK LRKGMPIGL RVTLRRDRMW IFLEKLLNVA LPRIRDFRGL NPNSFDGRGN YNLGLREQLI FPEITYDMVD ALRGMDIAVV T TAETDEEA RALLELLGFP FRK

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Macromolecule #5: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 18.567633 KDa
SequenceString:
SRIGRLPIPV PKGVSVEVAP GRVKVKGPKG ELEVPVSPEM RVVVEEGVVR VERPSDERRH KSLHGLTRTL IANAVKGVSE GYSKELLIK GIGYRARLVG RALELTVGFS HPVVVEPPEG ITFEVPEPTR VRVSGIDKQK VGQVAANIRA IRKPSAYHEK G IYYAGEPV RL

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Macromolecule #6: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 5.454388 KDa
SequenceString:
MKVILLEPLE NLGDVGQVVD VKPGYARNYL LPRGLAVLAT ESNLKALEAR

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Macromolecule #7: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 15.699658 KDa
SequenceString:
MKTYVPKQVE PRWVLIDAEG KTLGRLATKI ATLLRGKHRP DWTPNVAMGD FVVVVNADKI RVTGKKLEQK IYTRYSGYPG GLKKIPLEK MLATHPERVL EHAVKGMLPK GPLGRRLFKR LKVYAGPDHP HQAQRPEKL

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Macromolecule #8: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 13.323612 KDa
SequenceString:
MIQPQTYLEV ADNTGARKIM CIRVLKGSNA KYATVGDVIV ASVKEAIPRG AVKEGDVVKA VVVRTKKEIK RPDGSAIRFD DNAAVIINN QLEPRGTRVF GPVARELREK GFMKIVSLAP EVL

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Macromolecule #9: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 16.319142 KDa
SequenceString:
MKLSDLRPNP GANKRRKRVG RGPGSGHGKT ATRGHKGQKS RSGGLKDPRR FEGGRSTTLM RLPKRGMQGQ VPGEIKRPRY QGVNLKDLA RFEGEVTPEL LVRAGLLKKG YRLKILGEGE AKPLKVVAHA FSKSALEKLK AAGGEPVLLE A

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Macromolecule #10: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 15.993909 KDa
SequenceString:
MLMPRRMKYR KQQRGRLKGA TKGGDYVAFG DYGLVALEPA WITAQQIEAA RVAMVRHFRR GGKIFIRIFP DKPYTKKPLE VRMGKGKGN VEGYVAVVKP GRVMFEVAGV TEEQAMEALR IAGHKLPIKT KIVRRDAYDE AQ

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Macromolecule #11: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 13.618952 KDa
SequenceString:
RHLKSGRKLN RHSSHRLALY RNQAKSLLTH GRITTTVPKA KELRGFVDHL IHLAKRGDLH ARRLVLRDLQ DVKLVRKLFD EIAPRYRDR QGGYTRVLKL AERRRGDGAP LALVELVE

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Macromolecule #12: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 12.508649 KDa
SequenceString:
ARLTAYERRK FRVRNRIKRT GRLRLSVFRS LKHIYAQIID DEKGVTLVSA SSLALKLKGN KTEVARQVGR ALAEKALALG IKQVAFDRG PYKYHGRVKA LAEGAREGGL EF

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Macromolecule #13: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 16.218812 KDa
SequenceString:
MNRGALIKLV ESRYVRTDLP EFRPGDTVRV SYKVKEGNRT RIQDFEGIVI RIRRNGFNTT FTVRKVSYGV GVERIFPLHS PLIQKIDIV QRGRARRAKL YFIRNLSDRE IRRKLRADRK RIDQDRAAER AAKEEAQK

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Macromolecule #14: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 13.648081 KDa
SequenceString:
PRAKTGVVRR RKHKKILKLA KGYWGLRSKS FRKARETLFA AGNYAYAHRK RRKRDFRRLW IVRINAACRQ HGLNYSTFIH GLKKAGIEV DRKNLADLAV REPQVFAELV ERAKAAQG

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Macromolecule #15: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 11.069153 KDa
SequenceString:
MFAIVKTGGK QYRVEPGLKL RVEKLDAEPG ATVELPVLLL GGEKTVVGTP VVEGASVVAE VLGHGRGKKI LVSKFKAKVQ YRRKKGHRQ PYTELLIKEI RG

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Macromolecule #16: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 12.808055 KDa
SequenceString:
MEAKAIARYV RISPRKVRLV VDLIRGKSLE EARNILRYTN KRGAYFVAKV LESAAANAVN NHDMLEDRLY VKAAYVDEGP ALKRVLPRA RGRADIIKKR TSHITVILGE KHGK

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Macromolecule #17: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 10.273117 KDa
SequenceString:
TAYDVILAPV LSEKAYAGFA EGKYTFWVHP KATKTEIKNA VETAFKVKVV KVNTLHVRGK KKRLGRYLGK RPDRKKAIVQ VAPGQKIEA LEG

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Macromolecule #18: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 11.23871 KDa
SequenceString:
RVKMHVKKGD TVLVASGKYK GRVGKVKEVL PKKYAVIVEG VNIVKKAVRV SPKYPQGGFI EKEAPLHASK VRPICPACGK PTRVRKKFL ENGKKIRVCA KCG

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Macromolecule #19: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 20.26833 KDa
SequenceString:
MEYRLKAYYR EGEKPSALRR AGKLPGVMYN RHLNRKVYVD LVEFDKVFRQ ASIHHVIVLE LPDGQSLPTL VRQVNLDKRR RRPEHVDFF VLSDEPVEMY VPLRFVGTPA GVRAGGVLQE IHRDILVKVS PRNIPEFIEV DVSGLEIGDS LHASDLKLPP G VELAVSPE ETIAAVVPPE D

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Macromolecule #20: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 8.703032 KDa
SequenceString:
STRNGRDSQA KRLGVKRYEG QVVRAGNILV RQRGTRFKPG KNVGMGRDFT LFALVDGVVE FQDRGRLGRY VHVRPLA

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Macromolecule #21: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 10.872965 KDa
SequenceString:
SKVCEISGKR PIVANSIQRR GKAKREGGVG KKTTGISKRR QYPNLQKVRV RVAGQEITFR VAASHIPKVY ELVERAKGLK LEGLSPKEI KKELLKLL

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Macromolecule #22: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 8.296725 KDa
SequenceString:
SEVRKQLEEA RKLSPVELEK LVREKKRELM ELRFQASIGQ LSQNHKIRDL KRQIARLLTV LNEKRRQNA

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Macromolecule #23: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 6.667931 KDa
SequenceString:
PRLKVKLVKS PIGYPKDQKA ALKALGLRRL QQERVLEDTP AIRGNVEKVA HLVRVEVVE

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Macromolecule #24: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 7.377532 KDa
SequenceString:
MKEGIHPKLV PARIICGCGN VIETYSTKPE IYVEVCSKCH PFYTGQQRFV DTEGRVERFQ RRY

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Macromolecule #25: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 6.590854 KDa
SequenceString:
AKHPVPKKKT SKARRDARRS HHALTPPTLV PCPECKAMKP PHTVCPECGY YAGRKVLEV

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Macromolecule #26: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 5.602612 KDa
SequenceString:
LLLECTECKR RNYATEKNKR NTPNKLELRK YCPWCRKHTV HREVK

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Macromolecule #27: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 6.132449 KDa
SequenceString:
MKRTWQPNRR KRAKTHGFRA RMRTPGGRKV LKRRRQKGRW RLTPAVRKR

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Macromolecule #28: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 6.985579 KDa
SequenceString:
PKMKTHKGAK KRVKITASGK VVAMKTGKRH LNWQKSGKEI RQKGRKFVLA KPEAERIKLL L

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Macromolecule #31: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 4.304215 KDa
SequenceString:
KVRASVKRIC DKCKVIRRHG RVYVICENPK HKQRQG

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Macromolecule #32: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 27.330648 KDa
SequenceString: EITVKELLEA GVHFGHERKR WNPKFARYIY AERNGIHIID LQKTMEELER TFRFIEDLAM RGGTILFVGT KKQAQDIVRM EAERAGMPY VNQRWLGGML TNFKTISQRV HRLEELEALF ASPEIEERPK KEQVRLKHEL ERLQKYLSGF RLLKRLPDAI F VVDPTKEA ...String:
EITVKELLEA GVHFGHERKR WNPKFARYIY AERNGIHIID LQKTMEELER TFRFIEDLAM RGGTILFVGT KKQAQDIVRM EAERAGMPY VNQRWLGGML TNFKTISQRV HRLEELEALF ASPEIEERPK KEQVRLKHEL ERLQKYLSGF RLLKRLPDAI F VVDPTKEA IAVREARKLF IPVIALADTD SDPDLVDYII PGNDDAIRSI QLILSRAVDL IIQARGGVVE PSPSYALVQ

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Macromolecule #33: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 22.86243 KDa
SequenceString: GNKIHPIGFR LGITRDWESR WYAGKKQYRH LLLEDQRIRG LLEKELYSAG LARVDIERAA DNVAVTVHVA KPGVVIGRGG ERIRVLREE LAKLTGKNVA LNVQEVQNPN LSAPLVAQRV AEQIERRFAV RRAIKQAVQR VMESGAKGAK VIVSGRIGGA E QARTEWAA ...String:
GNKIHPIGFR LGITRDWESR WYAGKKQYRH LLLEDQRIRG LLEKELYSAG LARVDIERAA DNVAVTVHVA KPGVVIGRGG ERIRVLREE LAKLTGKNVA LNVQEVQNPN LSAPLVAQRV AEQIERRFAV RRAIKQAVQR VMESGAKGAK VIVSGRIGGA E QARTEWAA QGRVPLHTLR ANIDYGFALA RTTYGVLGVK AYIFLGEV

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Macromolecule #34: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus HB8 (bacteria) / Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 24.242254 KDa
SequenceString: GRYIGPVCRL CRREGVKLYL KGERCYSPKC AMERRPYPPG QHGQKRARRP SDYAVRLREK QKLRRIYGIS ERQFRNLFEE ASKKKGVTG SVFLGLLESR LDNVVYRLGF AVSRRQARQL VRHGHITVNG RRVDLPSYRV RPGDEIAVAE KSRNLELIRQ N LEAMKGRK ...String:
GRYIGPVCRL CRREGVKLYL KGERCYSPKC AMERRPYPPG QHGQKRARRP SDYAVRLREK QKLRRIYGIS ERQFRNLFEE ASKKKGVTG SVFLGLLESR LDNVVYRLGF AVSRRQARQL VRHGHITVNG RRVDLPSYRV RPGDEIAVAE KSRNLELIRQ N LEAMKGRK VGPWLSLDVE GMKGKFLRLP DREDLALPVN EQLVIEFYSR

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Macromolecule #35: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 16.460193 KDa
SequenceString:
DFEEKMILIR RTARMQAGGR RFRFGALVVV GDRQGRVGLG FGKAPEVPLA VQKAGYYARR NMVEVPLQNG TIPHEIEVEF GASKIVLKP AAPGTGVIAG AVPRAILELA GVTDILTKEL GSRNPINIAY ATMEALRQLR TKADVERLRK GE

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Macromolecule #36: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 11.988753 KDa
SequenceString:
MRRYEVNIVL NPNLDQSQLA LEKEIIQRAL ENYGARVEKV EELGLRRLAY PIAKDPQGYF LWYQVEMPED RVNDLARELR IRDNVRRVM VVKSQEPFLA NA

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Macromolecule #37: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 17.919775 KDa
SequenceString:
ARRRRAEVRQ LQPDLVYGDV LVTAFINKIM RDGKKNLAAR IFYDACKIIQ EKTGQEPLKV FKQAVENVKP RMEVRSRRVG GANYQVPME VSPRRQQSLA LRWLVQAANQ RPERRAAVRI AHELMDAAEG KGGAVKKKED VERMAEANRA YAHYRW

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Macromolecule #38: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 15.86857 KDa
SequenceString:
MLTDPIADML TRIRNATRVY KESTDVPASR FKEEILRILA REGFIKGYER VDVDGKPYLR VYLKYGPRRQ GPDPRPEQVI HHIRRISKP GRRVYVGVKE IPRVRRGLGI AILSTSKGVL TDREARKLGV GGELICEVW

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Macromolecule #39: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 14.279419 KDa
SequenceString:
EQYYGTGRRK EAVARVFLRP GNGKVTVNGQ DFNEYFQGLV RAVAALEPLR AVDALGHFDA YITVRGGGKS GQIDAIKLGI ARALVQYNP DYRAKLKPLG FLTRDARVVE RKKYGKHKAR RAPQYSKR

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Macromolecule #40: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 11.398308 KDa
SequenceString:
KIRIKLRGFD HKTLDASAQK IVEAARRSGA QVSGPIPLPT RVRRFTVIRG PFKHKDSREH FELRTHNRLV DIINPNRKTI EQLMTLDLP TGVEIEIKTV

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Macromolecule #41: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 12.519292 KDa
SequenceString:
KRQVASGRAY IHASYNNTIV TITDPDGNPI TWSSGGVIGY KGSRKGTPYA AQLAALDAAK KAMAYGMQSV DVIVRGTGAG REQAIRALQ ASGLQVKSIV DDTPVPHNGC RPKKKFRKA

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Macromolecule #42: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 13.875388 KDa
SequenceString:
PTINQLVRKG REKVRKKSKV PALKGAPFRR GVCTVVRTVT PKKPNSALRK VAKVRLTSGY EVTAYIPGEG HNLQEHSVVL IRGGRVKDL PGVRYHIVRG VYDAAGVKDR KKSRSKYGTK KPKEAA

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Macromolecule #43: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 13.308508 KDa
SequenceString:
ARIAGVEIPR NKRVDVALTY IYGIGKARAK EALEKTGINP ATRVKDLTEA EVVRLREYVE NTWKLEGELR AEVAANIKRL MDIGCYRGL RHRRGLPVRG QRTRTNARTR KGPRKTVA

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Macromolecule #44: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 7.027529 KDa
SequenceString:
ARKALIEKAK RTPKFKVRAY TRCVRCGRAR SVYRFFGLCR ICLRELAHKG QLPGVRKASW

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Macromolecule #45: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 10.447213 KDa
SequenceString:
PITKEEKQKV IQEFARFPGD TGSTEVQVAL LTLRINRLSE HLKVHKKDHH SHRGLLMMVG QRRRLLRYLQ REDPERYRAL IEKLGIRG

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Macromolecule #46: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 9.995546 KDa
SequenceString:
MVKIRLARFG SKHNPHYRIV VTDARRKRDG KYIEKIGYYD PRKTTPDWLK VDVERARYWL SVGAQPTDTA RRLLRQAGVF RQEA

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Macromolecule #47: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 11.880098 KDa
SequenceString:
PKKVLTGVVV SDKMQKTVTV LVERQFPHPL YGKVIKRSKK YLAHDPEEKY KLGDVVEIIE SRPISKRKRF RVLRLVESGR MDLVEKYLI RRQNYESLSK R

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Macromolecule #48: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 7.312723 KDa
SequenceString:
GEFDLRDYRN VEVLKRFLSE TGKILPRRRT GLSAKEQRIL AKTIKRARIL GLLPFTEKLV RK

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Macromolecule #49: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 8.872271 KDa
SequenceString:
KGVFVDDHLL EKVLELNAKG EKRLIKTWSR RSTIVPEMVG HTIAVYNGKQ HVPVYITENM VGHKLGEFAP TRTYRGHG

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Macromolecule #50: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 10.921086 KDa
SequenceString:
RNLSALKRHR QSLKRRLRNK AKKSAIKTLS KKAIQLAQEG KAEEALKIMR KAESLIDKAA KGSTLHKNAA ARRKSRLMRK VRQLLEAAG APLIGGGLSA

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Macromolecule #51: 30S ribosomal protein Thx

MacromoleculeName: 30S ribosomal protein Thx / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 3.089655 KDa
SequenceString:
GKGDRRTRRG KIWRGTYGKY RPRKK

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Macromolecule #52: Ribosome hibernation promoting factor

MacromoleculeName: Ribosome hibernation promoting factor / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 13.980993 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
NIYKLIGRNL EITDAIRDYV EKKLARLDRY QDGELMAKVV LSLAGSPHVE KKARAEIQVD LPGGLVRVEE EDADLYAAID RAVDRLETQ VKRFRERRYV GKRHSYQGPP PPEVRDLEAL RK

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Macromolecule #29: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 29 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 947.013 KDa
SequenceString: GGUCAAGAUG GUAAGGGCCC ACGGUGGAUG CCUCGGCACC CGAGCCGAUG AAGGACGUGG CUACCUGCGA UAAGCCAGGG GGAGCCGGU AGCGGGCGUG GAUCCCUGGA UGUCCGAAUG GGGGAACCCG GCCGGCGGGA ACGCCGGUCA CCGCGCUUUU U GCGCGGGG ...String:
GGUCAAGAUG GUAAGGGCCC ACGGUGGAUG CCUCGGCACC CGAGCCGAUG AAGGACGUGG CUACCUGCGA UAAGCCAGGG GGAGCCGGU AGCGGGCGUG GAUCCCUGGA UGUCCGAAUG GGGGAACCCG GCCGGCGGGA ACGCCGGUCA CCGCGCUUUU U GCGCGGGG GGAACCUGGG GAACUGAAAC AUCUCAGUAC CCAGAGGAGA GGAAAGAGAA AUCGACUCCC UGAGUAGCGG CG AGCGAAA GGGGACCAGC CUAAACCGUC CGGCUUGUCC GGGCGGGGUC GUGGGGCCCU CGGACACCGA AUCCCCAGCC UAG CCGAAG CUGUUGGGAA GCAGCGCCAG AGAGGGUGAA AGCCCCGUAG GCGAAAGGUG GGGGGAUAGG UGAGGGUACC CGAG UACCC CGUGGUUCGU GGAGCCAUGG GGGAAUCUGG GCGGACCACC GCCUAAGGCU AAGUACUCCG GGUGACCGAU AGCGC ACCA GUACCGUGAG GGAAAGGUGA AAAGAACCCC GGGAGGGGAG UGAAAUAGAG CCUGAAACCG UGGGCUUACA AGCAGU CAC GGCCCCGCAA GGGGUUGUGG CGUGCCUAUU GAAGCAUGAG CCGGCGACUC ACGGUCGUGG GCGAGCUUAA GCCGUUG AG GCGGAGGCGU AGGGAAACCG AGUCCGAACA GGGCGCAAAC GGCCCGCACG CGGGCCGAAA AGUCCGCGGC CGUGGACC C GAAACCGGGC GAGCUAGCCC UGGCCAGGGU GAAGCUGGGG UGAGACCCAG UGGAGGCCCG AACCGGUGGG GGAUGCAAA CCCCUCGGAU GAGCUGGGGC UAGGAGUGAA AAGCUAACCG AGCCCGGAGA UAGCUGGUUC UCCCCGAAAU GACUUUAGGG UCAGCCUCA GGCGCUGACU GGGGCCUGUA GAGCACUGAU AGGGCUAGGG GGCCCACCAG CCUACCAAAC CCUGUCAAAC U CCGAAGGG UCCCAGGUGG AGCCUGGGAG UGAGGGCGCG AGCGAUAACG UCCGCGUCCG AGCGCGGGAA CAACCGAGAC CG CCAGCUA AGGCCCCCAA GUCUGGGCUA AGUGGUAAAG GAUGUGGCGC CGCGAAGACA GCCAGGAUGU UGGCUUAGAA GCA GCCAUC AUUUAAAGAG UGCGUAAUAG CUCACUGGUC GAGUGGCGCC GCGCCGAAAA UGAUCGGGGC UUAAGCCCAG CGCC GAAGC UGCGGGUCUG GGGGAUGACC CCAGGCGGUA GGGGAGCGUU CCCGAUGCCG AUGAAGGCCG ACCCGCGAGG GCGGC UGGA GGUAAGGGAA GUGCGAAUGC CGGCAUGAGU AACGAUAAAG AGGGUGAGAA UCCCUCUCGC CGUAAGCCCA AGGGUU CCU ACGCAAUGGU CGUCAGCGUA GGGUUAGGCG GGACCUAAGG UGAAGCCGAA AGGCGUAGCC GAAGGGCAGC CGGUUAA UA UUCCGGCCCU UCCCGCAGGU GCGAUGGGGG GACGCUCUAG GCUAGGGGGA CCGGAGCCAU GGACGAGCCC GGCCAGAA G CGCAGGGUGG GAGGUAGGCA AAUCCGCCUC CCAACAAGCU CUGCGUGGUG GGGAAGCCCG UACGGGUGAC AACCCCCCG AAGCCAGGGA GCCAAGAAAA GCCUCUAAGC ACAACCUGCG GGAACCCGUA CCGCAAACCG ACACAGGUGG GCGGGUGCAA GAGCACUCA GGCGCGCGGG AGAACCCUCG CCAAGGAACU CUGCAAGUUG GCCCCGUAAC UUCGGGAGAA GGGGUGCUCC C UGGGGUGA UGAGCCCCGG GGAGCCGCAG UGAACAGGCU CUGGCGACUG UUUACCAAAA ACACAGCUCU CUGCGAACUC GU AAGAGGA GGUAUAGGGA GCGACGCUUG CCCGGUGCCG GAAGGUCAAG GGGAGGGGUG CAAGCCCCGA ACCGAAGCCC CGG UGAACG GCGGCCGUAA CUAUAACGGU CCUAAGGUAG CGAAAUUCCU UGUCGGGUAA GUUCCGACCU GCACGAAAAG CGUA ACGAC CGGAGCGCUG UCUCGGCGAG GGACCCGGUG AAAUUGAACU GGCCGUGAAG AUGCGGCCUA CCCGUGGCAG GACGA AAAG ACCCCGUGGA GCUUUACUGC AGCCUGGUGU UGGCUCUUGG UCGCGCCUGC GUAGGAUAGG UGGGAGCCUG UGAACC CCC GCCUCCGGGU GGGGGGGAGG CGCCGGUGAA AUACCACCCU GGCGCGGCUG GGGGCCUAAC CCUCGGAUGG GGGGACA GC GCUUGGCGGG CAGUUUGACU GGGGCGGUCG CCUCCUAAAA GGUAACGGAG GCGCCCAAAG GUCCCCUCAG GCGGGACG G AAAUCCGCCG GAGAGCGCAA GGGUAGAAGG GGGCCUGACU GCGAGGCCUG CAAGCCGAGC AGGGGCGAAA GCCGGGCCU AGUGAACCGG UGGUCCCGUG UGGAAGGGCC AUCGAUCAAC GGAUAAAAGU UACCCCGGGG AUAACAGGCU GAUCUCCCCC GAGCGUCCA CAGCGGCGGG GAGGUUUGGC ACCUCGAUGU CGGCUCGUCG CAUCCUGGGG CUGAAGAAGG UCCCAAGGGU U GGGCUGUU CGCCCAUUAA AGCGGCACGC GAGCUGGGUU CAGAACGUCG UGAGACAGUU CGGUCUCUAU CCGCCACGGG CG CAGGAGG CUUGAGGGGG GCUCUUCCUA GUACGAGAGG ACCGGAAGGG ACGCACCUCU GGUUUCCCAG CUGUCCCUCC AGG GGCAUA AGCUGGGUAG CCAUGUGCGG AAGGGAUAAC CGCUGAAAGC AUCUAAGCGG GAAGCCCGCC CCAAGAUGAG GCCU CCCAC GGCGUCAAGC CGGUAAGGAC CCGGGAAGAC CACCCGGUGG AUGGGCCGGG GGUGUAAGCG CCGCGAGGCG UUGAG CCGA CCGGUCCCAA UCGUCCGAGG UCUUGACC

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Macromolecule #30: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 30 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 39.540617 KDa
SequenceString:
AAUCCCCCGU GCCCAUAGCG GCGUGGAACC ACCCGUUCCC AUUCCGAACA CGGAAGUGAA ACGCGCCAGC GCCGAUGGUA CUGGGCGGG CGACCGCCUG GGAGAGUAGG UCGGUGCGGG GGA

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Macromolecule #53: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)
Molecular weightTheoretical: 489.002531 KDa
SequenceString: UGGAGAGUUU GAUCCUGGCU CAGGGUGAAC GCUGGCGGCG UGCCUAAGAC AUGCAAGUCG UGCGGGCCGC GGGGUUUUAC UCCGUGGUC AGCGGCGGAC GGGUGAGUAA CGCGUGGGUG ACCUACCCGG AAGAGGGGGA CAACCCGGGG AAACUCGGGC U AAUCCCCC ...String:
UGGAGAGUUU GAUCCUGGCU CAGGGUGAAC GCUGGCGGCG UGCCUAAGAC AUGCAAGUCG UGCGGGCCGC GGGGUUUUAC UCCGUGGUC AGCGGCGGAC GGGUGAGUAA CGCGUGGGUG ACCUACCCGG AAGAGGGGGA CAACCCGGGG AAACUCGGGC U AAUCCCCC AUGUGGACCC GCCCCUUGGG GUGUGUCCAA AGGGCUUUGC CCGCUUCCGG AUGGGCCCGC GUCCCAUCAG CU AGUUGGU GGGGUAAUGG CCCACCAAGG CGACGACGGG UAGCCGGUCU GAGAGGAUGG CCGGCCACAG GGGCACUGAG ACA CGGGCC CCACUCCUAC GGGAGGCAGC AGUUAGGAAU CUUCCGCAAU GGGCGCAAGC CUGACGGAGC GACGCCGCUU GGAG GAAGA AGCCCUUCGG GGUGUAAACU CCUGAACCCG GGACGAAACC CCCGACGAGG GGACUGACGG UACCGGGGUA AUAGC GCCG GCCAACUCCG UGCCAGCAGC CGCGGUAAUA CGGAGGGCGC GAGCGUUACC CGGAUUCACU GGGCGUAAAG GGCGUG UAG GCGGCCUGGG GCGUCCCAUG UGAAAGACCA CGGCUCAACC GUGGGGGAGC GUGGGAUACG CUCAGGCUAG ACGGUGG GA GAGGGUGGUG GAAUUCCCGG AGUAGCGGUG AAAUGCGCAG AUACCGGGAG GAACGCCGAU GGCGAAGGCA GCCACCUG G UCCACCCGUG ACGCUGAGGC GCGAAAGCGU GGGGAGCAAA CCGGAUUAGA UACCCGGGUA GUCCACGCCC UAAACGAUG CGCGCUAGGU CUCUGGGUCU CCUGGGGGCC GAAGCUAACG CGUUAAGCGC GCCGCCUGGG GAGUACGGCC GCAAGGCUGA AACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA GCAUGUGGUU UAAUUCGAAG CAACGCGAAG AACCUUACCA G GCCUUGAC AUGCUAGGGA ACCCGGGUGA AAGCCUGGGG UGCCCCGCGA GGGGAGCCCU AGCACAGGUG CUGCAUGGCC GU CGUCAGC UCGUGCCGUG AGGUGUUGGG UUAAGUCCCG CAACGAGCGC AACCCCCGCC GUUAGUUGCC AGCGGUUCGG CCG GGCACU CUAACGGGAC UGCCCGCGAA AGCGGGAGGA AGGAGGGGAC GACGUCUGGU CAGCAUGGCC CUUACGGCCU GGGC GACAC ACGUGCUACA AUGCCCACUA CAAAGCGAUG CCACCCGGCA ACGGGGAGCU AAUCGCAAAA AGGUGGGCCC AGUUC GGAU UGGGGUCUGC AACCCGACCC CAUGAAGCCG GAAUCGCUAG UAAUCGCGGA UCAGCCAUGC CGCGGUGAAU ACGUUC CCG GGCCUUGUAC ACACCGCCCG UCACGCCAUG GGAGCGGGCU CUACCCGAAG UCGCCGGGAG CCUACGGGCA GGCGCCG AG GGUAGGGCCC GUGACUGGGG CGAAGUCGUA ACAAGGUAGC UGUACCGGAA GGUGCGGCUG GAUC

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 1.06 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.28 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 45422

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