EMDB-51134: human 40S ribosome bound by a SKI238-exosome complex

Basic information

Entry

Database: EMDB / ID: EMD-51134

• Title: human 40S ribosome bound by a SKI238-exosome complex

Sample

• Complex: human 40S ribosome bound by a SKI238-exosome complex
  • RNA: x 2 types
  • Protein or peptide: x 48 types

• Keywords: RNA-binding / RNA-degradation / cytoplasm / helicase / RIBOSOME

Function / homology

Function:

DNA deamination / nucleolar exosome (RNase complex) / exoribonuclease II activity / exoribonuclease II / Dom34-Hbs1 complex / nuclear-transcribed mRNA catabolic process, no-go decay / CUT catabolic process / Ski complex / cytoplasmic exosome (RNase complex) / U1 snRNA 3'-end processing / U5 snRNA 3'-end processing / mRNA decay by 3' to 5' exoribonuclease / TRAMP-dependent tRNA surveillance pathway / exosome (RNase complex) / Cdc73/Paf1 complex / U4 snRNA 3'-end processing / nuclear polyadenylation-dependent rRNA catabolic process / poly(A)-dependent snoRNA 3'-end processing / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / nuclear exosome (RNase complex) / negative regulation of myeloid cell differentiation / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / ATF4 activates genes in response to endoplasmic reticulum stress / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / positive regulation of isotype switching / 3'-5' RNA helicase activity / histone mRNA catabolic process / nuclear mRNA surveillance / rRNA catabolic process / 7S RNA binding / mRNA 3'-UTR AU-rich region binding / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / isotype switching / positive regulation of respiratory burst involved in inflammatory response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / protein tyrosine kinase inhibitor activity / positive regulation of endodeoxyribonuclease activity / IRE1-RACK1-PP2A complex / nucleolus organization / positive regulation of Golgi to plasma membrane protein transport / TNFR1-mediated ceramide production / negative regulation of DNA repair / negative regulation of RNA splicing / ribosome disassembly / RNA catabolic process / supercoiled DNA binding / neural crest cell differentiation / NF-kappaB complex / cysteine-type endopeptidase activator activity involved in apoptotic process / oxidized purine DNA binding / positive regulation of ubiquitin-protein transferase activity / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / regulation of establishment of cell polarity / negative regulation of bicellular tight junction assembly / ubiquitin-like protein conjugating enzyme binding / negative regulation of phagocytosis / rRNA modification in the nucleus and cytosol / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / KSRP (KHSRP) binds and destabilizes mRNA / cytoplasmic side of rough endoplasmic reticulum membrane / maturation of 5.8S rRNA / laminin receptor activity / negative regulation of ubiquitin protein ligase activity / protein kinase A binding / ion channel inhibitor activity / Ribosomal scanning and start codon recognition / nuclear chromosome / pigmentation / Translation initiation complex formation / positive regulation of mitochondrial depolarization / positive regulation of T cell receptor signaling pathway / negative regulation of Wnt signaling pathway / fibroblast growth factor binding / mRNA catabolic process / Association of TriC/CCT with target proteins during biosynthesis / monocyte chemotaxis / positive regulation of activated T cell proliferation / negative regulation of translational frameshifting / TOR signaling / Protein hydroxylation / BH3 domain binding / SARS-CoV-1 modulates host translation machinery / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / iron-sulfur cluster binding / regulation of cell division / cellular response to ethanol / mTORC1-mediated signalling / Peptide chain elongation / nuclear-transcribed mRNA catabolic process / Selenocysteine synthesis / Formation of a pool of free 40S subunits / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Eukaryotic Translation Termination

Domain/homology:

Exosome complex component Rrp43 / : / Mammalian exosome complex component RRP40, N-terminal / HBS1-like protein, N-terminal domain superfamily / Ski3/TTC37 / HBS1-like protein, N-terminal / HBS1 N-terminus / Ski2, N-terminal domain / Ski2 N-terminal region / Exosome complex exonuclease RRP44, S1 domain / S1 domain / Exosome complex component RRP45 / Rrp40, S1 domain / : / Exosome complex component RRP40, S1 domain / Exosome complex component CSL4, C-terminal / Exosome complex component, N-terminal domain / Exosome complex component Csl4 / Exosome component EXOSC1/CSL4 / Exosome complex exonuclease RRP4 N-terminal region / RRP4, S1 domain / Rrp44-like cold shock domain / Rrp44-like cold shock domain / : / KH domain / Exosome complex RNA-binding protein 1/RRP40/RRP4 / Dis3-like cold-shock domain 2 / Dis3-like cold-shock domain 2 (CSD2) / : / : / rRNA-processing arch domain / Ribonuclease II/R, conserved site / Ribonuclease II family signature. / Mtr4-like, beta-barrel domain / : / Ribonuclease II/R / RNB domain / RNB / : / ATP-dependent RNA helicase Ski2, C-terminal / ATP-dependent RNA helicase Ski2-like / : / DSHCT (NUC185) domain / Exosome RNA helicase MTR4-like, stalk / DSHCT / : / Exoribonuclease, phosphorolytic domain 2 / 3' exoribonuclease family, domain 2 / : / GTP-eEF1A C-terminal domain-like / Exoribonuclease, phosphorolytic domain 1 / PNPase/RNase PH domain superfamily / Exoribonuclease, PH domain 2 superfamily / 3' exoribonuclease family, domain 1 / : / Tetratricopeptide repeat / K Homology domain, type 1 / Tetratricopeptide repeat / 40S ribosomal protein SA / K Homology domain, type 1 superfamily / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Ubiquitin-like protein FUBI / Tetratricopeptide repeat / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S2, eukaryotic / Translation elongation factor EFTu-like, domain 2 / S27a-like superfamily / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / : / Ribosomal protein S7e signature. / TPR repeat region circular profile. / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S25 / S25 ribosomal protein

Component:

Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS28 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1 / Exosome complex component RRP45 / Exosome complex component RRP4 / Exosome complex component RRP42 / Superkiller complex protein 2 / Exosome complex component MTR3 / Superkiller complex protein 3 / DIS3-like exonuclease 1 / Exosome complex component RRP43 / Superkiller complex protein 8 / Exosome complex component RRP41 / Exosome complex component RRP46 / Exosome complex component RRP40 / Exosome complex component CSL4 / HBS1-like protein

• Biological species: Homo sapiens (human) / Cricket paralysis virus
• Method: single particle reconstruction / cryo EM / Resolution: 3.4 Å
• Authors: Koegel A / Keidel A / Loukeri MJ / Kuhn CC / Langer LM / Schaefer IB / Conti E

Funding support

Germany, European Union, Denmark, 5 items

Organization	Grant number	Country
Max Planck Society		Germany
European Research Council (ERC)	740329	European Union
European Research Council (ERC)	101054447	European Union
German Research Foundation (DFG)	SFB1035	Germany
Novo Nordisk Foundation	31199	Denmark

• Citation: Journal: Nature / Year: 2024; Title: Structural basis of mRNA decay by the human exosome-ribosome supercomplex.; Authors: Alexander Kögel / Achim Keidel / Matina-Jasemi Loukeri / Christopher C Kuhn / Lukas M Langer / Ingmar B Schäfer / Elena Conti / ; Abstract: The interplay between translation and mRNA decay is widespread in human cells. In quality-control pathways, exonucleolytic degradation of mRNA associated with translating ribosomes is mediated largely by the cytoplasmic exosome, which includes the exoribonuclease complex EXO10 and the helicase complex SKI238 (refs. ). The helicase can extract mRNA from the ribosome and is expected to transfer it to the exoribonuclease core through a bridging factor, HBS1L3 (also known as SKI7), but the mechanisms of this molecular handover remain unclear. Here we reveal how human EXO10 is recruited by HBS1L3 (SKI7) to an active ribosome-bound SKI238 complex. We show that rather than a sequential handover, a direct physical coupling mechanism takes place, which culminates in the formation of a cytoplasmic exosome-ribosome supercomplex. Capturing the structure during active decay reveals a continuous path in which an RNA substrate threads from the 80S ribosome through the SKI2 helicase into the exoribonuclease active site of the cytoplasmic exosome complex. The SKI3 subunit of the complex directly binds to HBS1L3 (SKI7) and also engages a surface of the 40S subunit, establishing a recognition platform in collided disomes. Exosome and ribosome thus work together as a single structural and functional unit in co-translational mRNA decay, coordinating their activities in a transient supercomplex.

History

Deposition	Jul 23, 2024	-
Header (metadata) release	Oct 16, 2024	-
Map release	Oct 16, 2024	-
Update	Nov 20, 2024	-
Current status	Nov 20, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_51134.map.gz / Format: CCP4 / Size: 2.1 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 0.8512 Å

Density

Contour Level	By AUTHOR: 0.01
Minimum - Maximum	-0.012958453 - 0.05347155
Average (Standard dev.)	0.000065772525 (±0.0009892983)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 832 832 832 Spacing 832 832 832
Cell	A=B=C: 708.19836 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_51134_msk_1.map

Additional map: composite map human 40S ribosome bound to the...

• File: emd_51134_additional_1.map
• Annotation: composite map human 40S ribosome bound to the SKI238 complex and the exosome

Half map: #2

• File: emd_51134_half_map_1.map

Half map: #1

• File: emd_51134_half_map_2.map

Sample components

Entire : human 40S ribosome bound by a SKI238-exosome complex

• Entire: Name: human 40S ribosome bound by a SKI238-exosome complex

Components

• Complex: human 40S ribosome bound by a SKI238-exosome complex
  • RNA: CrPV-IRES RNA
  • Protein or peptide: 40S ribosomal protein S16
  • Protein or peptide: 40S ribosomal protein S17
  • Protein or peptide: 40S ribosomal protein S18
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: 40S ribosomal protein S28
  • Protein or peptide: 60S ribosomal protein L41
  • Protein or peptide: 40S ribosomal protein S29
  • Protein or peptide: Ubiquitin
  • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S6
  • RNA: 18S ribosomal RNA
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein SA
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S3a
  • Protein or peptide: 40S ribosomal protein S14
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: Helicase SKI2W
  • Protein or peptide: Superkiller complex protein 3
  • Protein or peptide: WD repeat-containing protein 61
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: Exosome complex component RRP42
  • Protein or peptide: Exosome complex component MTR3
  • Protein or peptide: Exosome complex component RRP40
  • Protein or peptide: Exosome complex component RRP4
  • Protein or peptide: Exosome complex component CSL4
  • Protein or peptide: Exosome complex component RRP45
  • Protein or peptide: Isoform 2 of HBS1-like protein
  • Protein or peptide: DIS3-like exonuclease 1
  • Protein or peptide: Exosome complex component RRP43
  • Protein or peptide: Exosome complex component RRP41
  • Protein or peptide: Exosome complex component RRP46
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S30
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S15

Supramolecule #1: human 40S ribosome bound by a SKI238-exosome complex

• Supramolecule: Name: human 40S ribosome bound by a SKI238-exosome complex / type: complex / ID: 1 / Parent: 0 ; Macromolecule list: #14, #34-#39, #41, #44, #46, #15, #47, #49-#50, #20, #24, #17, #27, #30, #18, #31, #19, #32, #21, #40, #22, #42, #9, #1-#2, #23, #43, #3, #11-#13, #4-#5, #10, #16, #6-#8, #25, #45, #26, #48, #28-#29, #33
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Superkiller complex protein 3

• Macromolecule: Name: Superkiller complex protein 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 176.06475 KDa
• Recombinant expression: Organism: Spodoptera frugiperda (fall armyworm)

Sequence

String:

GPDSMSSKEV KTALKSARDA IRNKEYKEAL KHCKTVLKQE KNNYNAWVFI GVAAAELEQP DQAQSAYKKA AELEPDQLLA WQGLANLYE KYNHINAKDD LPGVYQKLLD LYESVDKQKW CDVCKKLVDL YYQEKKHLEV ARTWHKLIKT RQEQGAENEE L HQLWRKLT QFLAESTEDQ NNETQQLLFT AFENALGLSD KIPSEDHQVL YRHFIQSLSK FPHESARLKK ACEGMINIYP TV QYPLEVL CLHLIESGNL TDEGQQYCCR LVEMDSKSGP GLIGLGIKAL QDKKYEDAVR NLTEGLKESP VCTSGWYHLA EAQ VKMHRP KEAVLSCSQA LKIVDNLGAS GNSLYQRNLC LHLKAEALIK LSDYDSSEEA IRTLDQISDA DNIPGLLVLK SLAY RNKGS FDEAAKIMED LLSSYPDLAE VHALEALIHF TKKDYLQAEK CFQRALEKDT EVAEYHYQLG LTYWFMGEET RKDKT KALT HFLKAARLDT YMGKVFCYLG HYYRDVVGDK NRARGCYRKA FELDDTDAES GAAAVDLSVE LEDMEMALAI LTTVTQ KAS AGTAKWAWLR RGLYYLKAGQ HSQAVADLQA ALRADPKDFN CWESLGEAYL SRGGYTTALK SFTKASELNP ESIYSVF KV AAIQQILGKY KEAVAQYQMI IKKKEDYVPA LKGLGECHLM MAKAALVDYL DGKAVDYIEK ALEYFTCALQ HRADVSCL W KLAGDACTCL YAVAPSKVNV HVLGVLLGQK EGKQVLKKNE LLHLGGRCYG RALKLMSTSN TWCDLGINYY RQAQHLAET GSNMNDLKEL LEKSLHCLKK AVRLDSNNHL YWNALGVVAC YSGIGNYALA QHCFIKSIQS EQINAVAWTN LGVLYLTNEN IEQAHEAFK MAQSLDPSYL MCWIGQALIA EAVGSYDTMD LFRHTTELNM HTEGALGYAY WVCTTLQDKS NRETELYQYN I LQMNAIPA AQVILNKYVE RIQNYAPAFT MLGYLNEHLQ LKKEAANAYQ RAILLLQTAE DQDTYNVAIR NYGRLLCSTG EY DKAIQAF KSTPLEVLED IIGFALALFM KGLYKESSKA YERALSIVES EQDKAHILTA LAITEYKQGK TDVAKTLLFK CSI LKEPTT ESLQALCALG LAMQDATLSK AALNELLKHI KHKDSNYQRC LLTSAIYALQ GRSVAVQKQI SKAVHSNPGD PALW SLLSR VVAQYAQRNA KGGVVAGNVA HILDSNHGKK ALLYTAVNQL AMGSSSAEDE KNTALKTIQK AALLSPGDPA IWAGL MAAC HADDKLALVN NTQPKRIDLY LALLSAVSAS IKDEKFFENY NQSLEKWSLS QAVTGLIDTG RISEAETLCT KNLKSN PDQ PAVILLLRQV QCKPLLESQK PLPDAVLEEL QKTVMSNSTS VPAWQWLAHV YQSQGMMRAA EMCYRKSLQL ASQRGSW SG KLSSLLRLAL LALKVCMANI SNDHWPSLVQ EATTEALKLC FCPLAVLLQA LLQFKRKMGA RETRRLLERV VYQPGYPK S IASTARWYLL RHLYAKDDYE LIDVLVNNAK THGDTRALEL NQRLSSQ

UniProtKB: Superkiller complex protein 3

Macromolecule #2: WD repeat-containing protein 61

• Macromolecule: Name: WD repeat-containing protein 61 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.617465 KDa
• Recombinant expression: Organism: Spodoptera frugiperda (fall armyworm)

Sequence

String:

MTNQYGILFK QEQAHDDAIW SVAWGTNKKE NSETVVTGSL DDLVKVWKWR DERLDLQWSL EGHQLGVVSV DISHTLPIAA SSSLDAHIR LWDLENGKQI KSIDAGPVDA WTLAFSPDSQ YLATGTHVGK VNIFGVESGK KEYSLDTRGK FILSIAYSPD G KYLASGAI DGIINIFDIA TGKLLHTLEG HAMPIRSLTF SPDSQLLVTA SDDGYIKIYD VQHANLAGTL SGHASWVLNV AF CPDDTHF VSSSSDKSVK VWDVGTRTCV HTFFDHQDQV WGVKYNGNGS KIVSVGDDQE IHIYDCPI

UniProtKB: Superkiller complex protein 8

Macromolecule #3: Exosome complex component RRP42

• Macromolecule: Name: Exosome complex component RRP42 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 32.216762 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSMASVTL SEAEKVYIVH GVQEDLRVDG RGCEDYRCVE VETDVVSNTS GSARVKLGHT DILVGVKAEM GTPKLEKPNE GYLEFFVDC SASATPEFEG RGGDDLGTEI ANTLYRIFNN KSSVDLKTLC ISPREHCWVL YVDVLLLECG GNLFDAISIA V KAALFNTR IPRVRVLEDE EGSKDIELSD DPYDCIRLSV ENVPCIVTLC KIGYRHVVDA TLQEEACSLA SLLVSVTSKG VV TCMRKVG KGSLDPESIF EMMETGKRVG KVLHASLQSV VHKEESLGPK RQKVGFLG

UniProtKB: Exosome complex component RRP42

Macromolecule #4: Exosome complex component CSL4

• Macromolecule: Name: Exosome complex component CSL4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.8351 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSMAPPVR YCIPGERLCN LEEGSPGSGT YTRHGYIFSS LAGCLMKSSE NGALPVVSVV RETESQLLPD VGAIVTCKVS SINSRFAKV HILYVGSMPL KNSFRGTIRK EDVRATEKDK VEIYKSFRPG DIVLAKVISL GDAQSNYLLT TAENELGVVV A HSESGIQM VPISWCEMQC PKTHTKEFRK VARVQPEFLQ T

UniProtKB: Exosome complex component CSL4

Macromolecule #5: Exosome complex component RRP45

• Macromolecule: Name: Exosome complex component RRP45 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.370312 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSMKETPL SNCERRFLLR AIEEKKRLDG RQTYDYRNIR ISFGTDYGCC IVELGKTRVL GQVSCELVSP KLNRATEGIL FFNLELSQM AAPAFEPGRQ SDLLVKLNRL MERCLRNSKC IDTESLCVVA GEKVWQIRVD LHLLNHDGNI IDAASIAAIV A LCHFRRPD VSVQGDEVTL YTPEERDPVP LSIHHMPICV SFAFFQQGTY LLVDPNEREE RVMDGLLVIA MNKHREICTI QS SGGIMLL KDQVLRCSKI AGVKVAEITE LILKALENDQ KVRKEGGKFG FAESIANQRI TAFKMEKAPI DTSDVEEKAE EII AEAEPP SEVVSTPVLW TPGTAQIGEG VENSWGDLED SEKEDDEGGG DQAIILDGIK MDTGVEVSDI GSQDAPIILS DSEE EEMII LEPDKNPKKI RTQTTSAKQE KAPSKKPVKR RKKKRAAN

UniProtKB: Exosome complex component RRP45

Macromolecule #6: Exosome complex component RRP43

• Macromolecule: Name: Exosome complex component RRP43 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.429893 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSMAAGFK TVEPLEYYRR FLKENCRPDG RELGEFRTTT VNIGSISTAD GSALVKLGNT TVICGVKAEF AAPSTDAPDK GYVVPNVDL PPLCSSRFRS GPPGEEAQVA SQFIADVIEN SQIIQKEDLC ISPGKLVWVL YCDLICLDYD GNILDACTFA L LAALKNVQ LPEVTINEET ALAEVNLKKK SYLNIRTHPV ATSFAVFDDT LLIVDPTGEE EHLATGTLTI VMDEEGKLCC LH KPGGSGL TGAKLQDCMS RAVTRHKEVK KLMDEVIKSM KPK

UniProtKB: Exosome complex component RRP43

Macromolecule #7: Exosome complex component RRP41

• Macromolecule: Name: Exosome complex component RRP41 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.416996 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAGLELLSDQ GYRVDGRRAG ELRKIQARMG VFAQADGSAY IEQGNTKALA VVYGPHEIRG SRARALPDRA LVNCQYSSAT FSTGERKRR PHGDRKSCEM GLQLRQTFEA AILTQLHPRS QIDIYVQVLQ ADGGTYAACV NAATLAVLDA GIPMRDFVCA C SAGFVDGT ALADLSHVEE AAGGPQLALA LLPASGQIAL LEMDARLHED HLERVLEAAA QAARDVHTLL DRVVRQHVRE AS ILLGD

UniProtKB: Exosome complex component RRP41

Macromolecule #8: Exosome complex component RRP46

• Macromolecule: Name: Exosome complex component RRP46 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.636312 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSMEEETH TDAKIRAENG TGSSPRGPGC SLRHFACEQN LLSRPDGSAS FLQGDTSVLA GVYGPAEVKV SKEIFNKATL EVILRPKIG LPGVAEKSRE RLIRNTCEAV VLGTLHPRTS ITVVLQVVSD AGSLLACCLN AACMALVDAG VPMRALFCGV A CALDSDGT LVLDPTSKQE KEARAVLTFA LDSVERKLLM SSTKGLYSDT ELQQCLAAAQ AASQHVFRFY RESLQRRYSK S

UniProtKB: Exosome complex component RRP46

Macromolecule #9: Helicase SKI2W

• Macromolecule: Name: Helicase SKI2W / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO; EC number: Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 137.913688 KDa
• Recombinant expression: Organism: Spodoptera frugiperda (fall armyworm)

Sequence

String:

MMETERLVLP PPDPLDLPLR AVELGCTGHW ELLNLPGAPE SSLPHGLPPC APDLQQEAEQ LFLSSPAWLP LHGVEHSARK WQRKTDPWS LLAVLGAPVP SDLQAQRHPT TGQILGYKEV LLENTNLSAT TSLSLRRPPG PASQSLWGNP TQYPFWPGGM D EPTITDLN TREEAEEEID FEKDLLTIPP GFKKGMDFAP KDCPTPAPGL LSLSCMLEPL DLGGGDEDEN EAVGQPGGPR GD TVSASPC SAPLARASSL EDLVLKEAST AVSTPEAPEP PSQEQWAIPV DATSPVGDFY RLIPQPAFQW AFEPDVFQKQ AIL HLERHD SVFVAAHTSA GKTVVAEYAI ALAQKHMTRT IYTSPIKALS NQKFRDFRNT FGDVGLLTGD VQLHPEASCL IMTT EILRS MLYSGSDVIR DLEWVIFDEV HYINDVERGV VWEEVLIMLP DHVSIILLSA TVPNALEFAD WIGRLKRRQI YVIST VTRP VPLEHYLFTG NSSKTQGELF LLLDSRGAFH TKGYYAAVEA KKERMSKHAQ TFGAKQPTHQ GGPAQDRGVY LSLLAS LRT RAQLPVVVFT FSRGRCDEQA SGLTSLDLTT SSEKSEIHLF LQRCLARLRG SDRQLPQVLH MSELLNRGLG VHHSGIL PI LKEIVEMLFS RGLVKVLFAT ETFAMGVNMP ARTVVFDSMR KHDGSTFRDL LPGEYVQMAG RAGRRGLDPT GTVILLCK G RVPEMADLHR MMMGKPSQLQ SQFRLTYTMI LNLLRVDALR VEDMMKRSFS EFPSRKDSKA HEQALAELTK RLGALEEPD MTGQLVDLPE YYSWGEELTE TQHMIQRRIM ESVNGLKSLS AGRVVVVKNQ EHHNALGVIL QVSSNSTSRV FTTLVLCDKP LSQDPQDRG PATAEVPYPD DLVGFKLFLP EGPCDHTVVK LQPGDMAAIT TKVLRVNGEK ILEDFSKRQQ PKFKKDPPLA A VTTAVQEL LRLAQAHPAG PPTLDPVNDL QLKDMSVVEG GLRARKLEEL IQGAQCVHSP RFPAQYLKLR ERMQIQKEME RL RFLLSDQ SLLLLPEYHQ RVEVLRTLGY VDEAGTVKLA GRVACAMSSH ELLLTELMFD NALSTLRPEE IAALLSGLVC QSP GDAGDQ LPNTLKQGIE RVRAVAKRIG EVQVACGLNQ TVEEFVGELN FGLVEVVYEW ARGMPFSELA GLSGTPEGLV VRCI QRLAE MCRSLRGAAR LVGEPVLGAK METAATLLRR DIVFAASLYT Q

UniProtKB: Superkiller complex protein 2

Macromolecule #10: Isoform 2 of HBS1-like protein

• Macromolecule: Name: Isoform 2 of HBS1-like protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO; EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.196131 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSSQCESP SLTELFQEHK ENNISQCFTL SDLCNQSSAS FTDLSLGSFP LSQLANRCQS SPGISELTGS LSSLAFHKAS PTRDLENLS LSELIAETID VDNSQIKKES FEVSLSEVRS PGIDSNIDLS VLIKNPDFVP KPVVDPSIAP SSRTKVLSSK L GKNSNFAK DNKKNNKGSL TRKPPFSLSW TKALAARPSA FASTLCLRYP LKSCKRRTLD LYKTFLYSRQ VQDVKDKEIS PL VAITPFD FKSASPDDIV KANQKKAFTR ELEVLFQ

UniProtKB: HBS1-like protein

Macromolecule #11: Exosome complex component MTR3

• Macromolecule: Name: Exosome complex component MTR3 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.267127 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MPGDHRRIRG PEESQPPQLY AADEEEAPGT RDPTRLRPVY ARAGLLSQAK GSAYLEAGGT KVLCAVSGPR QAEGGERGGG PAGAGGEAP AALRGRLLCD FRRAPFAGRR RRAPPGGCEE RELALALQEA LEPAVRLGRY PRAQLEVSAL LLEDGGSALA A ALTAAALA LADAGVEMYD LVVGCGLSLA PGPAPTWLLD PTRLEEERAA AGLTVALMPV LNQVAGLLGS GEGGLTESWA EA VRLGLEG CQRLYPVLQQ SLVRAARRRG AAAQP

UniProtKB: Exosome complex component MTR3

Macromolecule #12: Exosome complex component RRP40

• Macromolecule: Name: Exosome complex component RRP40 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.940502 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSMAEPAS VAAESLAGSR ARAARTVLGQ VVLPGEELLL PEQEDAEGPG GAVERPLSLN ARACSRVRVV CGPGLRRCGD RLLVTKCGR LRHKEPGSGS GGGVYWVDSQ QKRYVPVKGD HVIGIVTAKS GDIFKVDVGG SEPASLSYLS FEGATKRNRP N VQVGDLIY GQFVVANKDM EPEMVCIDSC GRANGMGVIG QDGLLFKVTL GLIRKLLAPD CEIIQEVGKL HPLEIVFGMN GR IWVKAKT IQQTLILANI LEACEHMTSD QRKQIFSRLA ES

UniProtKB: Exosome complex component RRP40

Macromolecule #13: Exosome complex component RRP4

• Macromolecule: Name: Exosome complex component RRP4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.190355 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPDSMAMEMR LPVARKPLSE RLGRDTKKHL VVPGDTITTD TGFMRGHGTY MGEEKLIASV AGSVERVNKL ICVKALKTRY IGEVGDIVV GRITEVQQKR WKVETNSRLD SVLLLSSMNL PGGELRRRSA EDELAMRGFL QEGDLISAEV QAVFSDGAVS L HTRSLKYG KLGQGVLVQV SPSLVKRQKT HFHDLPCGAS VILGNNGFIW IYPTPEHKEE EAGGFIANLE PVSLADREVI SR LRNCIIS LVTQRMMLYD TSILYCYEAS LPHQIKDILK PEIMEEIVME TRQRLLEQEG

UniProtKB: Exosome complex component RRP4

Macromolecule #15: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #16: DIS3-like exonuclease 1

• Macromolecule: Name: DIS3-like exonuclease 1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO / EC number: exoribonuclease II
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 125.229984 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MSAWSHPQFE KGGGSGGGSG GSAWSHPQFE KTAGLEVLFQ GPMLQKREKV LLLRTFQGRT LRIVREHYLR PCVPCHSPLC PQPAACSHD GKLLSSDVTH YVIPDWKVVQ DYLEILEFPE LKGIIFMQTA CQAVQHQRGR RQYNKLRNLL KDARHDCILF A NEFQQCCY LPRERGESME KWQTRSIYNA AVWYYHHCQD RMPIVMVTED EEAIQQYGSE TEGVFVITFK NYLDNFWPDL KA AHELCDS ILQSRREREN ESQESHGKEY PEHLPLEVLE AGIKSGRYIQ GILNVNKHRA QIEAFVRLQG ASSKDSDLVS DIL IHGMKA RNRSIHGDVV VVELLPKNEW KGRTVALCEN DCDDKASGES PSEPMPTGRV VGILQKNWRD YVVTFPSKEE VQSQ GKNAQ KILVTPWDYR IPKIRISTQQ AETLQDFRVV VRIDSWESTS VYPNGHFVRV LGRIGDLEGE IATILVENSI SVIPF SEAQ MCEMPVNTPE SPWKVSPEEE QKRKDLRKSH LVFSIDPKGC EDVNDTLSVR TLNNGNLELG VHIADVTHFV APNSYI DIE ARTRATTYYL ADRRYDMLPS VLSADLCSLL GGVDRYAVSI MWELDKASYE IKKVWYGRTI IRSAYKLFYE AAQELLD GN LSVVDDIPEF KDLDEKSRQA KLEELVWAIG KLTDIARHVR AKRDGCGALE LEGVEVCVQL DDKKNIHDLI PKQPLEVH E TVAECMILAN HWVAKKIWES FPHQALLRQH PPPHQEFFSE LRECAKAKGF FIDTRSNKTL ADSLDNANDP HDPIVNRLL RSMATQAMSN ALYFSTGSCA EEEFHHYGLA LDKYTHFTSP IRRYSDIVVH RLLMAAISKD KKMEIKGNLF SNKDLEELCR HINNRNQAA QHSQKQSTEL FQCMYFKDKD PATEERCISD GVIYSIRTNG VLLFIPRFGI KGAAYLKNKD GLVISCGPDS C SEWKPGSL QRFQNKITST TTDGESVTFH LFDHVTVRIS IQASRCHSDT IRLEIISNKP YKIPNTELIH QSSPLLKSEL VK EVTKSVE EAQLAQEVKV NIIQEEYQEY RQTKGRSLYT LLEEIRDLAL LDVSNNYGI

UniProtKB: DIS3-like exonuclease 1

Macromolecule #18: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 32.883938 KDa

Sequence

String:

MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGATTDWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #19: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #20: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 31.376516 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG NRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW MPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #21: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.729369 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPTGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #22: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4, X isoform

Macromolecule #23: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #24: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #25: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #26: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #27: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #28: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #29: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #30: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.548896 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VQASNCDEPM YVKLVEALLA EHQINLIKVD DNKKLGEWV GLCKIDREGN PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #31: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #32: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #33: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.076207 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #34: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.477377 KDa

Sequence

String:

MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #35: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.578156 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGPV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #36: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.759777 KDa

Sequence

String:

MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #37: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.091562 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #38: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #39: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.124389 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #40: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #41: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.844666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #42: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.463333 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #43: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.776224 KDa

Sequence

String:

MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #44: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

UniProtKB: Small ribosomal subunit protein eS26

Macromolecule #45: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #46: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #47: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #48: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.668938 KDa

Sequence

String:

KVHGSLARAG KVRGQTPKVA KQEKKKKKTG RAKRRMQYNR RFVNVVPTFG KKKGPNANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #49: Ubiquitin

• Macromolecule: Name: Ubiquitin / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.004041 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #50: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #14: CrPV-IRES RNA

• Macromolecule: Name: CrPV-IRES RNA / type: rna / ID: 14 / Number of copies: 1
• Source (natural): Organism: Cricket paralysis virus
• Molecular weight: Theoretical: 79.333953 KDa

Sequence

String:

AAAAAUGUGA UCUUGCUGUG UAAAUACAAU UUUGAGAGGU UAAUAAAUUA CAAGUAGUGC UAUUUUUGUA UUUAGGUUAG CUAUUUAGC UUUACGUUCC AGGAUGCCUA GUGGCAGCCC CACAAUAUCC AGGAAGCCCU CUCUGCGGUU CGAAAAACCU U UUUAGGUA UUUGGUUUUU CUAAGAAAUU UACCUAUUUU UAAAAAAAUU UUUUUUUUUU UUUUUUUUUU UUUUCUCCUC UU UUUUUUU UUU

Macromolecule #17: 18S ribosomal RNA

• Macromolecule: Name: 18S ribosomal RNA / type: rna / ID: 17 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 602.752875 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC CACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGGCGC UGACCCCCUU CGCGGGGGGG AUGCGUGCAU UUAUCAGAUC AAAACCAACC CG GUCAGCC CCUCUCCGGC CCCGGCCGGG GGGCGGGCGC CGGCGGCUUU GGUGACUCUA GAUAACCUCG GGCCGAUCGC ACG CCCCCC GUGGCGGCGA CGACCCAUUC GAACGUCUGC CCUAUCAACU UUCGAUGGUA GUCGCCGUGC CUACCAUGGU GACC ACGGG UGACGGGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCG CGCA AAUUACCCAC UCCCGACCCG GGGAGGUAGU GACGAAAAAU AACAAUACAG GACUCUUUCG AGGCCCUGUA AUUGGA AUG AGUCCACUUU AAAUCCUUCC GCGAGGAUCC AUUGGAGGGC AAGUCUGGUG CCAGCAGCCG CGGUAAUUCC AGCUCCA AU AGCGUAUAUU AAAGUUGCUG CAGUUAAAAA GCUCGUAGUU GGAUCUUGGG AGCGGGCGGG CGGUCCGCCG CGAGGCGA G CCACCGCCCG UCCCCGCCCC UUGCCUCUCG GCGCCCCCUC GAUGCUCUUA GCUGAGUGUC CCGCGGGGCC CGAAGCAUU UACUUUGAAA AAAUUAGAGU GUUCAAAGCA GGCCCGAGCC GCCUGGAUAC CGCAGCUAGG AAUAAUGGAA UAGGACCGCG GUUCUAUUU UGUUGGUUUU CGGAACUGAG GCCAUGAUUA AGAGGGACGG CCGGGGGCAU UCGUAUUGCG CCGCUAGAGG U GAAAUUCU UGGACCGGCG CAAGACGGAC CAGAGCGAAA GCAUUUGCCA AGAAUGUUUU CAUUAAUCAA GAACGAAAGU CG GAGGUUC GAAGACGAUC AGAUACCGUC GUAGUUCCGA CCAUAAACGA UGCCGACUGG CGAUGCGGCG GCGUUAUUCC CAU GACCCG CCGGGCAGCU UCCGGGAAAC CAAAGUCUUU GGGUUCCGGG GGGAGUAUGG UUGCAAAGCU GAAACUUAAA GGAA UUGAC GGAAGGGCAC CACCAGGAGU GGAGCCUGCG GCUUAAUUUG ACUCAACACG GGAAACCUCA CCCGGCCCGG ACACG GACA GGAUUGACAG AUUGAUAGCU CUUUCUCGAU UCCGUGGGUG GUGGUGCAUG GCCGUUCUUA GUUGGUGGAG CGAUUU GUC UGGUUAAUUC CGAUAACGAA CGAGACUCUG GCAUGCUAAC UAGUUACGCG ACCCCCGAGC GGUCGGCGUC CCCCAAC UU CUUAGAGGGA CAAGUGGCGU UCAGCCACCC GAGAUUGAGC AAUAACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUG C ACGCGCGCUA CACUGACUGG CUCAGCGUGU GCCUACCCUA CGCCGGCAGG CGCGGGUAAC CCGUUGAACC CCAUUCGUG AUGGGGAUCG GGGAUUGCAA UUAUUCCCCA UGAACGAGGA AUUCCCAGUA AGUGCGGGUC AUAAGCUUGC GUUGAUUAAG UCCCUGCCC UUUGUACACA CCGCCCGUCG CUACUACCGA UUGGAUGGUU UAGUGAGGCC CUCGGAUCGG CCCCGCCGGG G UCGGCCCA CGGCCCUGGC GGAGCGCUGA GAAGACGGUC GAACUUGACU AUCUAGAGGA AGUAAAAGUC GUAACAAGGU UU CCGUAGG UGAACCUGCG GAAGGAUCAU UA

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/1 / Mesh: 200 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 64.2 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.6 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

7qds

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 53460
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD