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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-electron tomogram of ATG2A and small unilamellar vesicles | |||||||||
![]() | Cryo-electron tomogram of ATG2A and small unilamellar vesicles (denoised using IsoNet) | |||||||||
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![]() | autophagy / intracellular trafficking / membranes / vesicles / LIPID TRANSPORT | |||||||||
Biological species | ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Dahmane S / Wang N / Stjepanovic G / Carlson LA | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for lipid transfer by the ATG2A-ATG9A complex Authors: Wang N / Dahmane S / Ti R / Mai X / Zhu L / Carlson LA / Stjepanovic G | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 212.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.4 KB 9.4 KB | Display Display | ![]() |
Images | ![]() | 191.1 KB | ||
Filedesc metadata | ![]() | 3.5 KB | ||
Others | ![]() | 217.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 329.5 KB | Display | ![]() |
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Full document | ![]() | 329 KB | Display | |
Data in XML | ![]() | 4.7 KB | Display | |
Data in CIF | ![]() | 5.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Cryo-electron tomogram of ATG2A and small unilamellar vesicles (denoised using IsoNet) | ||||||||||||||||||||
Voxel size | X=Y=Z: 11.12 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: un-denoised map
File | emd_50658_additional_1.map | ||||||||||||
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Annotation | un-denoised map | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : ATG2A and small unilamellar vesicles
Entire | Name: ATG2A and small unilamellar vesicles |
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Components |
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-Supramolecule #1: ATG2A and small unilamellar vesicles
Supramolecule | Name: ATG2A and small unilamellar vesicles / type: complex / ID: 1 / Parent: 0 / Details: Purified human protein mixed with vesicles (SUVs). |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | electron tomography |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE-PROPANE |
Sectioning | Other: NO SECTIONING |
Fiducial marker | Manufacturer: CMC-Utrecht / Diameter: 10 nm |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 2.5 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 3.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Number images used: 41 |
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