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- EMDB-48918: CryoEM structure of the Azotobacter vinelandii flagellar filament -
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Open data
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Basic information
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Title | CryoEM structure of the Azotobacter vinelandii flagellar filament | |||||||||
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![]() | Flagellum / STRUCTURAL PROTEIN | |||||||||
Function / homology | ![]() bacterial-type flagellum / structural molecule activity / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 2.82 Å | |||||||||
![]() | Warmack RA | |||||||||
Funding support | ![]()
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![]() | ![]() Title: CryoEM-enabled visual proteomics reveals de novo structures of oligomeric protein complexes Authors: Warmack RA | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 3.3 GB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.5 KB 13.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 32.5 KB | Display | ![]() |
Images | ![]() | 73.2 KB | ||
Filedesc metadata | ![]() | 5.1 KB | ||
Others | ![]() ![]() | 3.3 GB 3.3 GB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 43.1 KB | Display | |
Data in CIF | ![]() | 58 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9n59MC ![]() 9n4vC ![]() 9n4wC ![]() 9n4yC ![]() 9n5aC ![]() 9nsvC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.65 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_48918_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_48918_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Filamentous assembly of the Azotobacter vinelandii flagellar filament
Entire | Name: Filamentous assembly of the Azotobacter vinelandii flagellar filament |
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Components |
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-Supramolecule #1: Filamentous assembly of the Azotobacter vinelandii flagellar filament
Supramolecule | Name: Filamentous assembly of the Azotobacter vinelandii flagellar filament type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Flagellin
Macromolecule | Name: Flagellin / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 58.040414 KDa |
Sequence | String: MPVINTNITS LIAQKNLSSS QSALTTAMER LSSGMRINSA KDDAAGQAIA NRMSSQITGL TQAQRNANDG ISVAQTTEGA LDQINDNLQ RIRELTVQAQ NGTNSSEDLT SIQNEIAQRL DEIDRISQET EFNGVKVLSK DTNLNIQVGA NDGQSIGISL K QINAKTLG ...String: MPVINTNITS LIAQKNLSSS QSALTTAMER LSSGMRINSA KDDAAGQAIA NRMSSQITGL TQAQRNANDG ISVAQTTEGA LDQINDNLQ RIRELTVQAQ NGTNSSEDLT SIQNEIAQRL DEIDRISQET EFNGVKVLSK DTNLNIQVGA NDGQSIGISL K QINAKTLG LDGFNVDGSG ATQNSTATVT SLEGAGAVKN STTGNYVLTT TFDEASLERM LGEMKDGDVV TVGSGASTAV EY TFNTASG SFTYTANATN AAAYGDLADE LIPPTGSSIT GTYEFADGNV ATFAVDASGK LTLDGQAAYV TATGELTNNA TSG ATQATL EDLFATVGAG AAGTDTSARS LTVGGVTYTG AGTTAGLSYT DTATSSDVLA ASASTVASIE MHNGITSATI DFDN TGAQS STGTQVYVDE DGQLTTVGSY TTTFAVNADT GEVTVVDNSD TAGDYALEEG ATVYVGSNGR LTTSTTSKGD VTEDP LAAL DAALASVDAL RSDLGAIQNR FDSAINNLST TTTNLSAARS RIEDADYAVE VANMTKAQIL QQAGTSVLAQ ANQVPQ SVL SLLG UniProtKB: Flagellin |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | helical array |
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Sample preparation
Concentration | 0.75 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE-PROPANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: -2.5 µm / Nominal defocus min: -0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |