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- EMDB-4716: BACTERIOPHAGE SPP1 MATURE CAPSID PROTEIN -

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Basic information

Entry
Database: EMDB / ID: EMD-4716
TitleBACTERIOPHAGE SPP1 MATURE CAPSID PROTEIN
Map data
Sample
  • Virus: Bacillus phage SPP1 (virus)
    • Protein or peptide: Major capsid protein
Function / homologyMajor capsid protein 13-like / Major capsid protein 13-like / T=7 icosahedral viral capsid / viral capsid / Major capsid protein
Function and homology information
Biological speciesBacillus phage SPP1 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.0 Å
AuthorsIgnatiou A / El Sadek Fadel M / Buerger J / Mielke T / Topf M / Tavares P
CitationJournal: Nat Commun / Year: 2019
Title: Structural transitions during the scaffolding-driven assembly of a viral capsid.
Authors: Athanasios Ignatiou / Sandrine Brasilès / Mehdi El Sadek Fadel / Jörg Bürger / Thorsten Mielke / Maya Topf / Paulo Tavares / Elena V Orlova /
Abstract: Assembly of tailed bacteriophages and herpesviruses starts with formation of procapsids (virion precursors without DNA). Scaffolding proteins (SP) drive assembly by chaperoning the major capsid ...Assembly of tailed bacteriophages and herpesviruses starts with formation of procapsids (virion precursors without DNA). Scaffolding proteins (SP) drive assembly by chaperoning the major capsid protein (MCP) to build an icosahedral lattice. Here we report near-atomic resolution cryo-EM structures of the bacteriophage SPP1 procapsid, the intermediate expanded procapsid with partially released SPs, and the mature capsid with DNA. In the intermediate state, SPs are bound only to MCP pentons and to adjacent subunits from hexons. SP departure results in the expanded state associated with unfolding of the MCP N-terminus and straightening of E-loops. The newly formed extensive inter-capsomere bonding appears to compensate for release of SPs that clasp MCP capsomeres together. Subsequent DNA packaging instigates bending of MCP A domain loops outwards, closing the hexons central opening and creating the capsid auxiliary protein binding interface. These findings provide a molecular basis for the sequential structural rearrangements during viral capsid maturation.
History
DepositionMar 19, 2019-
Header (metadata) releaseOct 23, 2019-
Map releaseOct 23, 2019-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 400
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 400
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6r3a
  • Surface level: 400
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6r3a
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4716.png

Downloads & links

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Map

FileDownload / File: emd_4716.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.31 Å
Density
Contour LevelBy AUTHOR: 4.5 / Movie #1: 400
Minimum - Maximum0 - 1751.3058
Average (Standard dev.)20.39052 (±96.73284)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-298-298-298
Dimensions600600600
Spacing600600600
CellA=B=C: 785.99994 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.311.311.31
M x/y/z600600600
origin x/y/z0.0000.0000.000
length x/y/z786.000786.000786.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS-298-298-298
NC/NR/NS600600600
D min/max/mean0.0001751.30620.391

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Supplemental data

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Sample components

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Entire : Bacillus phage SPP1

EntireName: Bacillus phage SPP1 (virus)
Components
  • Virus: Bacillus phage SPP1 (virus)
    • Protein or peptide: Major capsid protein

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Supramolecule #1: Bacillus phage SPP1

SupramoleculeName: Bacillus phage SPP1 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all
Details: Mature assembly, icosahedral symmetry has been applied
NCBI-ID: 10724 / Sci species name: Bacillus phage SPP1 / Sci species strain: SPP1sus70 / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Bactria (plant) / Strain: Bacillus
Molecular weightTheoretical: 30 MDa
Virus shellShell ID: 1 / Name: capsid / Diameter: 610.0 Å / T number (triangulation number): 7

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Macromolecule #1: Major capsid protein

MacromoleculeName: Major capsid protein / type: protein_or_peptide / ID: 1 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Bacillus phage SPP1 (virus)
Molecular weightTheoretical: 35.258426 KDa
SequenceString: AYTKISDVIV PELFNPYVIN TTTQLSAFFQ SGIAATDDEL NALAKKAGGG STLNMPYWND LDGDSQVLND TDDLVPQKIN AGQDKAVLI LRGNAWSSHD LAATLSGSDP MQAIGSRVAA YWAREMQKIV FAELAGVFSN DDMKDNKLDI SGTADGIYSA E TFVDASYK ...String:
AYTKISDVIV PELFNPYVIN TTTQLSAFFQ SGIAATDDEL NALAKKAGGG STLNMPYWND LDGDSQVLND TDDLVPQKIN AGQDKAVLI LRGNAWSSHD LAATLSGSDP MQAIGSRVAA YWAREMQKIV FAELAGVFSN DDMKDNKLDI SGTADGIYSA E TFVDASYK LGDHESLLTA IGMHSATMAS AVKQDLIEFV KDSQSGIRFP TYMNKRVIVD DSMPVETLED GTKVFTSYLF GA GALGYAE GQPEVPTETA RNALGSQDIL INRKHFVLHP RGVKFTENAM AGTTPTDEEL ANGANWQRVY DPKKIRIVQF KHR LQA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
GridModel: Quantifoil R3/3 / Material: COPPER / Mesh: 400 / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK II
DetailsMature virions of the SPP1 bacteriophage

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated defocus max: 3.5 µm / Calibrated defocus min: 1.0 µm / Calibrated magnification: 31000 / Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.3 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 31000
Specialist opticsChromatic aberration corrector: none
Sample stageSpecimen holder model: GATAN 910 MULTI-SPECIMEN SINGLE TILT CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
TemperatureMin: 50.0 K / Max: 60.0 K
DetailsSample preparation was screened in advance
Image recordingFilm or detector model: GATAN K2 BASE (4k x 4k) / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Digitization - Sampling interval: 5.0 µm / Number grids imaged: 1 / Number real images: 7000 / Average exposure time: 5.0 sec. / Average electron dose: 25.0 e/Å2
Details: Images were collected in movie mode, 25 images during 5 s
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 200 / Details: 6000 particles were picked in total
CTF correctionSoftware - Name: CTFFIND (ver. 4) / Details: Software used Imagic 5
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: IMAGIC (ver. 5) / Details: Icosahedral symmetry was applied
Final 3D classificationNumber classes: 3000 / Avg.num./class: 3 / Software - Name: IMAGIC (ver. 5)
Details: In the final reconstruction were used single images
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: IMAGIC (ver. 5)
Final reconstructionApplied symmetry - Point group: I (icosahedral) / Algorithm: EXACT BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: IMAGIC (ver. 5) / Number images used: 4500
DetailsMovie frames were aligned using MOTIONCORR-2

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-6r3a:
BACTERIOPHAGE SPP1 MATURE CAPSID PROTEIN

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