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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Intact V-ATPase State 1 and synaptophysin complex in mouse brain isolated synaptic vesicles | |||||||||
![]() | Full map of V-ATPase State 1 synaptophysin complex in wild-type ISVs. | |||||||||
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![]() | V-ATPase / synaptic vesicle / MEMBRANE PROTEIN | |||||||||
機能・相同性 | ![]() Ion channel transport / regulation of opioid receptor signaling pathway / Amino acids regulate mTORC1 / clathrin-sculpted glutamate transport vesicle membrane / Transferrin endocytosis and recycling / Insulin receptor recycling / eye pigmentation / RHOA GTPase cycle / central nervous system maturation / Metabolism of Angiotensinogen to Angiotensins ...Ion channel transport / regulation of opioid receptor signaling pathway / Amino acids regulate mTORC1 / clathrin-sculpted glutamate transport vesicle membrane / Transferrin endocytosis and recycling / Insulin receptor recycling / eye pigmentation / RHOA GTPase cycle / central nervous system maturation / Metabolism of Angiotensinogen to Angiotensins / transporter activator activity / negative regulation of autophagic cell death / rostrocaudal neural tube patterning / plasma membrane proton-transporting V-type ATPase complex / cellular response to increased oxygen levels / positive regulation of transforming growth factor beta1 production / proton-transporting V-type ATPase, V1 domain / synaptic vesicle lumen acidification / endosome to plasma membrane protein transport / intracellular organelle / ROS and RNS production in phagocytes / proton-transporting V-type ATPase, V0 domain / extrinsic component of synaptic vesicle membrane / P-type proton-exporting transporter activity / lysosomal lumen acidification / clathrin-coated vesicle membrane / endosomal lumen acidification / vacuolar proton-transporting V-type ATPase, V0 domain / vacuolar proton-transporting V-type ATPase, V1 domain / vacuolar transport / proton-transporting V-type ATPase complex / neuron spine / head morphogenesis / osteoclast development / regulation of short-term neuronal synaptic plasticity / protein localization to cilium / neuron projection terminus / vacuolar proton-transporting V-type ATPase complex / dendritic spine membrane / regulation of cellular pH / syntaxin-1 binding / vacuolar acidification / regulation of synaptic vesicle exocytosis / : / cholesterol binding / ATPase complex / presynaptic active zone / regulation of neuronal synaptic plasticity / autophagosome membrane / microvillus / regulation of MAPK cascade / ATPase activator activity / synaptic vesicle endocytosis / excitatory synapse / positive regulation of Wnt signaling pathway / cilium assembly / transmembrane transporter complex / endomembrane system / regulation of macroautophagy / angiotensin maturation / ATP metabolic process / axon terminus / H+-transporting two-sector ATPase / proton transmembrane transport / ruffle / phagocytic vesicle / RNA endonuclease activity / proton-transporting ATPase activity, rotational mechanism / Neutrophil degranulation / endoplasmic reticulum-Golgi intermediate compartment membrane / proton-transporting ATP synthase activity, rotational mechanism / SH2 domain binding / receptor-mediated endocytosis / SNARE binding / secretory granule / regulation of long-term neuronal synaptic plasticity / neuromuscular junction / Schaffer collateral - CA1 synapse / terminal bouton / transmembrane transport / cilium / small GTPase binding / synaptic vesicle membrane / endocytosis / positive regulation of canonical Wnt signaling pathway / melanosome / synaptic vesicle / apical part of cell / myelin sheath / presynapse / signaling receptor activity / presynaptic membrane / ATPase binding / cell body / chemical synaptic transmission / postsynaptic membrane / intracellular iron ion homeostasis / receptor-mediated endocytosis of virus by host cell / 加水分解酵素; エステル加水分解酵素 / postsynaptic density 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.3 Å | |||||||||
![]() | Wang C / Jiang W / Yang K / Wang X / Guo Q / Brunger AT | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure and topography of the synaptic V-ATPase-synaptophysin complex. 著者: Chuchu Wang / Wenhong Jiang / Jeremy Leitz / Kailu Yang / Luis Esquivies / Xing Wang / Xiaotao Shen / Richard G Held / Daniel J Adams / Tamara Basta / Lucas Hampton / Ruiqi Jian / Lihua Jiang ...著者: Chuchu Wang / Wenhong Jiang / Jeremy Leitz / Kailu Yang / Luis Esquivies / Xing Wang / Xiaotao Shen / Richard G Held / Daniel J Adams / Tamara Basta / Lucas Hampton / Ruiqi Jian / Lihua Jiang / Michael H B Stowell / Wolfgang Baumeister / Qiang Guo / Axel T Brunger / ![]() ![]() ![]() ![]() 要旨: Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here we discovered a ...Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here we discovered a well-defined interface between the synaptic vesicle V-ATPase and synaptophysin by in situ cryo-electron tomography and single-particle cryo-electron microscopy of functional synaptic vesicles isolated from mouse brains. The synaptic vesicle V-ATPase is an ATP-dependent proton pump that establishes the proton gradient across the synaptic vesicle, which in turn drives the uptake of neurotransmitters. Synaptophysin and its paralogues synaptoporin and synaptogyrin belong to a family of abundant synaptic vesicle proteins whose function is still unclear. We performed structural and functional studies of synaptophysin-knockout mice, confirming the identity of synaptophysin as an interaction partner with the V-ATPase. Although there is little change in the conformation of the V-ATPase upon interaction with synaptophysin, the presence of synaptophysin in synaptic vesicles profoundly affects the copy number of V-ATPases. This effect on the topography of synaptic vesicles suggests that synaptophysin assists in their biogenesis. In support of this model, we observed that synaptophysin-knockout mice exhibit severe seizure susceptibility, suggesting an imbalance of neurotransmitter release as a physiological consequence of the absence of synaptophysin. | |||||||||
履歴 |
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構造の表示
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-EMDBアーカイブ
マップデータ | ![]() | 322.3 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 38.9 KB 38.9 KB | 表示 表示 | ![]() |
画像 | ![]() | 126.8 KB | ||
Filedesc metadata | ![]() | 9.9 KB | ||
その他 | ![]() ![]() ![]() ![]() | 296.1 MB 274.3 MB 318 MB 318 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9brtMC ![]() 9braC ![]() 9brqC ![]() 9brrC ![]() 9brsC ![]() 9bruC ![]() 9bryC ![]() 9brzC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | Full map of V-ATPase State 1 synaptophysin complex in wild-type ISVs. | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.11138 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-追加マップ: EMhancer map of V-ATPase State 1 synaptophysin complex...
ファイル | emd_44843_additional_1.map | ||||||||||||
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注釈 | EMhancer map of V-ATPase State 1 synaptophysin complex in wild-type ISVs. | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Unsharpened map of V-ATPase State 1
ファイル | emd_44843_additional_2.map | ||||||||||||
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注釈 | Unsharpened map of V-ATPase State 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half map of V-ATPase State 1 synaptophysin complex in wild-type ISVs.
ファイル | emd_44843_half_map_1.map | ||||||||||||
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注釈 | Half map of V-ATPase State 1 synaptophysin complex in wild-type ISVs. | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half map of V-ATPase State 1 synaptophysin complex in wild-type ISVs.
ファイル | emd_44843_half_map_2.map | ||||||||||||
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注釈 | Half map of V-ATPase State 1 synaptophysin complex in wild-type ISVs. | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Mouse brain isolated glutamatergic synaptic vesicles
+超分子 #1: Mouse brain isolated glutamatergic synaptic vesicles
+分子 #1: V-type proton ATPase subunit E 1
+分子 #2: V-type proton ATPase subunit G 2
+分子 #3: V-type proton ATPase catalytic subunit A
+分子 #4: V-type proton ATPase subunit B, brain isoform
+分子 #5: V-type proton ATPase subunit C 1
+分子 #6: V-type proton ATPase subunit D
+分子 #7: V-type proton ATPase subunit H
+分子 #8: V-type proton ATPase subunit F
+分子 #9: V-type proton ATPase 116 kDa subunit a 1
+分子 #10: V-type proton ATPase 21 kDa proteolipid subunit c''
+分子 #11: V-type proton ATPase subunit d 1
+分子 #12: V-type proton ATPase 16 kDa proteolipid subunit c
+分子 #13: Renin receptor cytoplasmic fragment
+分子 #14: V-type proton ATPase subunit S1
+分子 #15: Ribonuclease kappa
+分子 #16: Synaptophysin
+分子 #17: V-type proton ATPase subunit e 2
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | cell |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
詳細 | The specimen state should be an intact subcellular component. |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 4.0 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: OTHER |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 4.3 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 17889 |
初期 角度割当 | タイプ: RANDOM ASSIGNMENT |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
-原子モデル構築 1
精密化 | プロトコル: FLEXIBLE FIT |
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得られたモデル | ![]() PDB-9brt: |