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- EMDB-4370: Unique features of mammalian mitochondrial translation initiation... -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-4370

Title

Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit.

Map data

Sample

Complex: mammalian mitochondrial translation initiation complex
- Complex: mammalian mitochondrial ribosome
  - Protein or peptide: x 52 types
  - RNA: x 2 types
- Complex: Translation initiation factor IF-2, mitochondrial
  - Protein or peptide: x 1 types
- Complex: tRNA
  - RNA: x 1 types
Ligand: x 8 types

Keywords

translation initiation / initiation factor IF2 / mitochondria / membrane targeting / RIBOSOME

Function / homology

Function and homology information

Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / translation factor activity, RNA binding / mitochondrial translational termination / mitochondrial transcription / mitochondrial translational elongation / ribonuclease III activity / translation release factor activity, codon nonspecific / Mitochondrial translation initiation ...
Similarity search - Function

Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / : / Elongation factor G domain 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / : ...Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / : / Elongation factor G domain 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / : / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L37, mitochondrial / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L28 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L35, mitochondrial / Ribosomal protein L51, mitochondrial / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Ribosomal protein L37/S30 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / : / : / Mitochondrial 28S ribosomal protein S30 (PDCD9) / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / : / MRPL44, double-stranded RNA binding domain / : / MRPL44 dsRNA-binding domain / Large ribosomal subunit protein mL44, endonuclease domain / : / : / Large ribosomal subunit protein bL9m C-terminal domain / Large ribosomal subunit protein bL9m N-terminal domain / Tim44-like domain / Tim44-like domain / Tim44 / : / : / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / Mitochondrial ribosomal protein L46 NUDIX / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal subunit 39S / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / 39S ribosomal protein L46, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein, eukaryotic / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / PEBP-like superfamily / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily / TGS-like / TGS domain profile. / TGS / Peptide chain release factor class I / RF-1 domain / Ribonuclease III, endonuclease domain superfamily / Double stranded RNA-binding domain (dsRBD) profile. / Double-stranded RNA-binding domain / : / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Beta-grasp domain superfamily / NTF2-like domain superfamily / NUDIX hydrolase-like domain superfamily / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily
Similarity search - Domain/homology

Large ribosomal subunit protein mL49 / Large ribosomal subunit protein mL62 / Large ribosomal subunit protein uL18m / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein bL20m / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L23, mitochondrial / Large ribosomal subunit protein bL34m ...
Similarity search - Component

Biological species

Sus scrofa (pig) /

Homo sapiens (human)

Method

single particle reconstruction / cryo EM / Resolution: 3.2 Å

Authors

Kummer E / Leibundgut M

Funding support

Switzerland, 3 items

Organization	Grant number	Country
European Molecular Biology Organization	ALTF 1196-2014	Switzerland
Swiss National Science Foundation	310030B_163478	Switzerland
Swiss National Science Foundation	138262	Switzerland

Citation

Journal: Nature / Year: 2018
Title: Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM.
Authors: Kummer E / Leibundgut M / Rackham O / Lee RG / Boehringer D / Filipovska A / Ban N

History

Deposition	Apr 13, 2018	-
Header (metadata) release	May 23, 2018	-
Map release	Aug 8, 2018	-
Update	May 15, 2024	-
Current status	May 15, 2024	Processing site: PDBe / Status: Released

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Structure visualization

Movie	Surface view with section colored by density value Surface level: 0.12 Imaged by UCSF Chimera Download Surface view colored by height Surface level: 0.12 Imaged by UCSF Chimera Download Surface view with fitted model Atomic models: PDB-6gb2 Surface level: 0.12 Imaged by UCSF Chimera Download Movie viewer
Structure viewer	EM map: SurfViewMolmilJmol/JSmol

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Downloads & links

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EMDB archive

Map data	emd_4370.map.gz	15.6 MB		EMDB map data format
Header (meta data)	emd-4370-v30.xml emd-4370.xml	88.4 KB 88.4 KB	Display Display	EMDB header
Images	emd_4370.png	149.9 KB
Masks	emd_4370_msk_1.map	84.6 MB		Mask map
Filedesc metadata	emd-4370.cif.gz	17.2 KB
Others	emd_4370_half_map_1.map.gz emd_4370_half_map_2.map.gz	71.8 MB 71.8 MB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-4370 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4370	HTTPS FTP

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Validation report

Summary document	emd_4370_validation.pdf.gz	435.6 KB	Display	EMDB validaton report
Full document	emd_4370_full_validation.pdf.gz	434.7 KB	Display
Data in XML	emd_4370_validation.xml.gz	11.6 KB	Display
Arichive directory	https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4370 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4370	HTTPS FTP

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Related structure data

Related structure data	6gb2MC 4368C 4369C 6gawC 6gazC C: citing same article (ref.) M: atomic model generated by this map
Similar structure data	3842 12567 3843 7bl3-d 10076 10977 4531 4434 2787 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#253 - Jan 2021 Expressome similarity (11) #10 - Oct 2000 Ribosome similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (7) #81 - Sep 2006 Elongation Factors similarity (3) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (5) #121 - Jan 2010 70S Ribosomes similarity (8) #105 - Sep 2008 Ribonuclease A similarity (3) #104 - Aug 2008 Selenocysteine Synthase similarity (3) #98 - Feb 2008 Small Interfering RNA similarity (1) #117 - Sep 2009 Xanthine Oxidoreductase similarity (1) #157 - Jan 2013 Transfer-Messenger RNA similarity (4) #245 - May 2020 Spliceosomes similarity (3) #161 - May 2013 Ricin similarity (3) #266 - Feb 2022 Oligosaccharyltransferase similarity (2) #154 - Oct 2012 Citric Acid Cycle similarity (1) #82 - Oct 2006 Cytochrome p450 similarity (1) #44 - Aug 2003 Calmodulin similarity (1) #145 - Jan 2012 Messenger RNA Capping similarity (1) #201 - Sep 2016 Isoprene Synthase similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1)

EMDB pages

EMDB (EBI/PDBe) /

EMDataResource

Related items in Molecule of the Month

#253 - Jan 2021
Expressome
similarity (11)
#10 - Oct 2000
Ribosome
similarity (1)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (7)
#81 - Sep 2006
Elongation Factors
similarity (3)
#16 - Apr 2001
Aminoacyl-tRNA Synthetases
similarity (5)
#121 - Jan 2010
70S Ribosomes
similarity (8)
#105 - Sep 2008
Ribonuclease A
similarity (3)
#104 - Aug 2008
Selenocysteine Synthase
similarity (3)
#98 - Feb 2008
Small Interfering RNA
similarity (1)
#117 - Sep 2009
Xanthine Oxidoreductase
similarity (1)
#157 - Jan 2013
Transfer-Messenger RNA
similarity (4)
#245 - May 2020
Spliceosomes
similarity (3)
#161 - May 2013
Ricin
similarity (3)
#266 - Feb 2022
Oligosaccharyltransferase
similarity (2)
#154 - Oct 2012
Citric Acid Cycle
similarity (1)
#82 - Oct 2006
Cytochrome p450
similarity (1)
#44 - Aug 2003
Calmodulin
similarity (1)
#145 - Jan 2012
Messenger RNA Capping
similarity (1)
#201 - Sep 2016
Isoprene Synthase
similarity (1)
#241 - Jan 2020
Twenty Years of Molecules
similarity (1)

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Map

File

Download / File: emd_4370.map.gz / Format: CCP4 / Size: 84.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Voxel size

X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.12 / Movie #1: 0.12
Minimum - Maximum	-0.3282438 - 0.65797997
Average (Standard dev.)	0.0017977863 (±0.0235527)

Symmetry

Space group: 1

Details

EMDB XML:

Map geometry

Axis order	X	Y	Z
Origin	0	0	0
Dimensions	281	281	281
Spacing	281	281	281

Cell

A=B=C: 390.59 Å
α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	281	281	281
origin x/y/z	0.000	0.000	0.000
length x/y/z	390.590	390.590	390.590
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	281	281	281
D min/max/mean	-0.328	0.658	0.002

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Supplemental data

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Sample components

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Entire : mammalian mitochondrial translation initiation complex

Entire	Name: mammalian mitochondrial translation initiation complex
Components	Complex: mammalian mitochondrial translation initiation complex Complex: mammalian mitochondrial ribosome Protein or peptide: Mitochondrial ribosomal protein L12 Protein or peptide: Mitochondrial ribosomal protein L27 Protein or peptide: Mitochondrial ribosomal protein L28 Protein or peptide: Mitochondrial ribosomal protein L47 Protein or peptide: 'Mitochondrial ribosomal protein L30 Protein or peptide: 'Mitochondrial ribosomal protein L55 Protein or peptide: Mitochondrial ribosomal protein L32 Protein or peptide: Mitochondrial ribosomal protein L33 Protein or peptide: Mitochondrial ribosomal protein L34 Protein or peptide: Mitochondrial ribosomal protein L35 Protein or peptide: Ribosomal protein RNA: 16S ribosomal RNA, mitochondrial RNA: CP tRNAPhe, mitochondrial Protein or peptide: Mitochondrial ribosomal protein L2 Protein or peptide: ICT1 Protein or peptide: Mitochondrial ribosomal protein L4 Protein or peptide: Mitochondrial ribosomal protein L9 Protein or peptide: Mitochondrial ribosomal protein L10 Protein or peptide: Mitochondrial ribosomal protein L11 Protein or peptide: Mitochondrial ribosomal protein L13 Protein or peptide: Mitochondrial ribosomal protein L14 Protein or peptide: Mitochondrial ribosomal protein L15 Protein or peptide: Mitochondrial ribosomal protein L16 Protein or peptide: Mitochondrial ribosomal protein L17 Protein or peptide: Mitochondrial ribosomal protein L18 Protein or peptide: Mitochondrial ribosomal protein L19 Protein or peptide: Mitochondrial ribosomal protein L20 Protein or peptide: Mitochondrial ribosomal protein L21 Protein or peptide: Mitochondrial ribosomal protein L22 Protein or peptide: Mitochondrial ribosomal protein L23 Protein or peptide: Mitochondrial ribosomal protein L24 Protein or peptide: Mitochondrial ribosomal protein L37 Protein or peptide: Mitochondrial ribosomal protein L38 Protein or peptide: Mitochondrial ribosomal protein L39 Protein or peptide: Mitochondrial ribosomal protein L40 Protein or peptide: Mitochondrial ribosomal protein L41 Protein or peptide: Mitochondrial ribosomal protein L42 Protein or peptide: Mitochondrial ribosomal protein L43 Protein or peptide: Mitochondrial ribosomal protein L44 Protein or peptide: Mitochondrial ribosomal protein L45 Protein or peptide: Mitochondrial ribosomal protein L46 Protein or peptide: Mitochondrial ribosomal protein L48 Protein or peptide: Mrpl34 Protein or peptide: Mitochondrial ribosomal protein L50 Protein or peptide: Mitochondrial ribosomal protein L51 Protein or peptide: Mitochondrial ribosomal protein L52 Protein or peptide: mL53, MRPL53 Protein or peptide: Uncharacterized protein Protein or peptide: Mitochondrial ribosomal protein L57 Protein or peptide: Mitochondrial ribosomal protein L58 Protein or peptide: 'Mitochondrial ribosomal protein L59 Protein or peptide: mL65, MRPS30 Protein or peptide: Mitochondrial ribosomal protein S18A Protein or peptide: unassigned secondary structure elements Complex: Translation initiation factor IF-2, mitochondrial Protein or peptide: Translation initiation factor IF-2, mitochondrial Complex: tRNA RNA: P-site fMet-tRNAMet, mitochondrial Ligand: MAGNESIUM ION Ligand: ZINC ION Ligand: GUANOSINE-5'-MONOPHOSPHATE Ligand: SPERMINE Ligand: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE Ligand: SODIUM ION Ligand: N-FORMYLMETHIONINE Ligand: water

Entire

Name: mammalian mitochondrial translation initiation complex

Components

Complex: mammalian mitochondrial translation initiation complex
- Complex: mammalian mitochondrial ribosome
  - Protein or peptide: Mitochondrial ribosomal protein L12
  - Protein or peptide: Mitochondrial ribosomal protein L27
  - Protein or peptide: Mitochondrial ribosomal protein L28
  - Protein or peptide: Mitochondrial ribosomal protein L47
  - Protein or peptide: 'Mitochondrial ribosomal protein L30
  - Protein or peptide: 'Mitochondrial ribosomal protein L55
  - Protein or peptide: Mitochondrial ribosomal protein L32
  - Protein or peptide: Mitochondrial ribosomal protein L33
  - Protein or peptide: Mitochondrial ribosomal protein L34
  - Protein or peptide: Mitochondrial ribosomal protein L35
  - Protein or peptide: Ribosomal protein
  - RNA: 16S ribosomal RNA, mitochondrial
  - RNA: CP tRNAPhe, mitochondrial
  - Protein or peptide: Mitochondrial ribosomal protein L2
  - Protein or peptide: ICT1
  - Protein or peptide: Mitochondrial ribosomal protein L4
  - Protein or peptide: Mitochondrial ribosomal protein L9
  - Protein or peptide: Mitochondrial ribosomal protein L10
  - Protein or peptide: Mitochondrial ribosomal protein L11
  - Protein or peptide: Mitochondrial ribosomal protein L13
  - Protein or peptide: Mitochondrial ribosomal protein L14
  - Protein or peptide: Mitochondrial ribosomal protein L15
  - Protein or peptide: Mitochondrial ribosomal protein L16
  - Protein or peptide: Mitochondrial ribosomal protein L17
  - Protein or peptide: Mitochondrial ribosomal protein L18
  - Protein or peptide: Mitochondrial ribosomal protein L19
  - Protein or peptide: Mitochondrial ribosomal protein L20
  - Protein or peptide: Mitochondrial ribosomal protein L21
  - Protein or peptide: Mitochondrial ribosomal protein L22
  - Protein or peptide: Mitochondrial ribosomal protein L23
  - Protein or peptide: Mitochondrial ribosomal protein L24
  - Protein or peptide: Mitochondrial ribosomal protein L37
  - Protein or peptide: Mitochondrial ribosomal protein L38
  - Protein or peptide: Mitochondrial ribosomal protein L39
  - Protein or peptide: Mitochondrial ribosomal protein L40
  - Protein or peptide: Mitochondrial ribosomal protein L41
  - Protein or peptide: Mitochondrial ribosomal protein L42
  - Protein or peptide: Mitochondrial ribosomal protein L43
  - Protein or peptide: Mitochondrial ribosomal protein L44
  - Protein or peptide: Mitochondrial ribosomal protein L45
  - Protein or peptide: Mitochondrial ribosomal protein L46
  - Protein or peptide: Mitochondrial ribosomal protein L48
  - Protein or peptide: Mrpl34
  - Protein or peptide: Mitochondrial ribosomal protein L50
  - Protein or peptide: Mitochondrial ribosomal protein L51
  - Protein or peptide: Mitochondrial ribosomal protein L52
  - Protein or peptide: mL53, MRPL53
  - Protein or peptide: Uncharacterized protein
  - Protein or peptide: Mitochondrial ribosomal protein L57
  - Protein or peptide: Mitochondrial ribosomal protein L58
  - Protein or peptide: 'Mitochondrial ribosomal protein L59
  - Protein or peptide: mL65, MRPS30
  - Protein or peptide: Mitochondrial ribosomal protein S18A
  - Protein or peptide: unassigned secondary structure elements
- Complex: Translation initiation factor IF-2, mitochondrial
  - Protein or peptide: Translation initiation factor IF-2, mitochondrial
- Complex: tRNA
  - RNA: P-site fMet-tRNAMet, mitochondrial
Ligand: MAGNESIUM ION
Ligand: ZINC ION
Ligand: GUANOSINE-5'-MONOPHOSPHATE
Ligand: SPERMINE
Ligand: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
Ligand: SODIUM ION
Ligand: N-FORMYLMETHIONINE
Ligand: water

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Supramolecule #1: mammalian mitochondrial translation initiation complex

Supramolecule	Name: mammalian mitochondrial translation initiation complex type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
Molecular weight	Theoretical: 2.85 MDa

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Supramolecule #2: mammalian mitochondrial ribosome

Supramolecule	Name: mammalian mitochondrial ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#13, #15-#55
Source (natural)	Organism: Sus scrofa (pig)

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Supramolecule #3: Translation initiation factor IF-2, mitochondrial

Supramolecule	Name: Translation initiation factor IF-2, mitochondrial / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #14
Source (natural)	Organism: Homo sapiens (human)

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Supramolecule #4: tRNA

Supramolecule	Name: tRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #56
Source (natural)	Organism: Homo sapiens (human)

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Macromolecule #1: Mitochondrial ribosomal protein L12

Macromolecule	Name: Mitochondrial ribosomal protein L12 / type: protein_or_peptide / ID: 1 Details: Unassigned residues have been built as poly-alanine. Number of copies: 7 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 21.268975 KDa
Sequence	String: MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL ...String: MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL PQEIKANVPK AEAEKIKAAL EAVGGTVVLE UniProtKB: Large ribosomal subunit protein bL12m

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Macromolecule #2: Mitochondrial ribosomal protein L27

Macromolecule	Name: Mitochondrial ribosomal protein L27 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 15.943688 KDa
Sequence	String: MALAVLALRT RAAVTALLSP PQAAALAVRY ASKKTGGSSK NLGGKSPGKR FGIKKMEGHY VHAGNILATQ RHFRWHPGAH VGLGKNKCL YALEEGVVRY TKEVYVPNPS NSEAVDLVTR LPQGAVLYKT FVHVVPAKPE GTFKLVAML UniProtKB: Large ribosomal subunit protein bL27m

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Macromolecule #3: Mitochondrial ribosomal protein L28

Macromolecule	Name: Mitochondrial ribosomal protein L28 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 30.148953 KDa
Sequence	String: MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR ...String: MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR LARQDPQLHP DDPARRAAIY DRYKAFVIPE AEAEWVGLTL DEAVEKQRLL EEKDPIPLFK IFVEELLGQL QQ QALSEPA VVQTRASRK UniProtKB: Large ribosomal subunit protein bL28m

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Macromolecule #4: Mitochondrial ribosomal protein L47

Macromolecule	Name: Mitochondrial ribosomal protein L47 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 30.085088 KDa
Sequence	String: MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG ...String: MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG AWRRDIFGRI IWHKFKQWPI PWYLNKKYNR KRFFAMPYVE RFVRMRIEKQ ARIKARKRSL ERKKEKFLQE KF PHLSETQ KSSHV UniProtKB: Large ribosomal subunit protein uL29m

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Macromolecule #5: 'Mitochondrial ribosomal protein L30

Macromolecule	Name: 'Mitochondrial ribosomal protein L30 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 18.58584 KDa
Sequence	String: MAGILRSVVQ RPPGRLQTVT KGMESLICTD WIRHKFTRSR IPDKVFQPSP EDHEKYGGDP QYPHKLHIVT RIKSTKRRPY WEKDIIKML GLEKAHTPQV HKNIPSVNAK LKVVKHLIRI KPLKLPQGLP TEEDMANTCL KSNGELVVRW LLNPANQEAR K S UniProtKB: Large ribosomal subunit protein uL30m

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Macromolecule #6: 'Mitochondrial ribosomal protein L55

Macromolecule	Name: 'Mitochondrial ribosomal protein L55 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 15.156537 KDa
Sequence	String: MAAKGSLLRL LWQRVVTGAA PESCRHLYTS SWRADCNRAL LTRLHRQTYA RLYPVLLVKQ DGSTIHIRYR EPRRMLTMPV DLDSLSPEE RRARFRKREA KFKEKKEEPE LSDDFDVEQY KRFWTKK UniProtKB: Mitochondrial ribosomal protein L55

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Macromolecule #7: Mitochondrial ribosomal protein L32

Macromolecule	Name: Mitochondrial ribosomal protein L32 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 21.53793 KDa
Sequence	String: MASAMLVLVV PPWPAARGLL RNWWEQLQRK LQHNRLGLPL HPWGPALAVQ GPAICTEPAN DTNGSKAISS LLDSVFWMAA PKNRRSIEV NRCRRRNPQK LIKVKNNIDV CPECGHLKQK HILCGYCYEK VRKETAEIRR QMGKQEGGPF RAPTTETVVL Y SGETPSEQ DQGKRIIERE RKRPSWFTQN

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Macromolecule #8: Mitochondrial ribosomal protein L33

Macromolecule	Name: Mitochondrial ribosomal protein L33 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 7.508097 KDa
Sequence	String: MFLSAVTFAK SKSKTILVKM MSQAGTGFSF NTKRSRLREK LTLLHYDPVV KKKVLFVEQK KIRSL UniProtKB: Large ribosomal subunit protein bL33m

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Macromolecule #9: Mitochondrial ribosomal protein L34

Macromolecule	Name: Mitochondrial ribosomal protein L34 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 10.871729 KDa
Sequence	String: MAFLARSVGR LLDPVSRSAA LVGGRWLQPQ AWLGFPDTWG LPAMQQTRGK ARGNEYQPSN IKRKHKHGWV RRLRTPTGVQ VILRRMHKG RKSLSH UniProtKB: Large ribosomal subunit protein bL34m

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Macromolecule #10: Mitochondrial ribosomal protein L35

Macromolecule	Name: Mitochondrial ribosomal protein L35 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 21.548543 KDa
Sequence	String: MAASAFAGAV RAATGILRPL HILASSAYQN CAKNACLSSV LSSRHFSHIQ TSALLSAPRL ITSVRNLMCG PTAPVLNRVA PLLPHVLKP PVRTVTYFSS RKGKRKTVKA VIYRFLRLHS GLWLRRKAGY KKKLWKKTAA RKRRLREFVF CNKTQSKLLD K MTTSFWKR RNWYADDPYQ KYQDRTNLKV UniProtKB: Large ribosomal subunit protein bL35m

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Macromolecule #11: Ribosomal protein

Macromolecule	Name: Ribosomal protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 10.841016 KDa
Sequence	String: MATAFLRTVL SAVGPLLHLG GRPLSTFAAG PPRAALAVGA QPSPAAALLS ARPLLGPQPA LGFKTKGVLK KRCRDCYLVK RRGRWFIYC KTNPKHKQRQ M UniProtKB: Ribosomal protein

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Macromolecule #14: Translation initiation factor IF-2, mitochondrial

Macromolecule	Name: Translation initiation factor IF-2, mitochondrial / type: protein_or_peptide / ID: 14 Details: Unassigned residues have been built as poly-alanine. Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Homo sapiens (human)
Molecular weight	Theoretical: 72.811219 KDa
Recombinant expression	Organism: Escherichia coli (E. coli)
Sequence	String: SGGSGSGWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKV RKNKDAVRRP QADPALLTPR SPVVTIMGHV DHGKTTLLDK FRKTQVAAVE TGGITQHIGA FLVSLPSGEK I TFLDTPGH ...String: SGGSGSGWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKV RKNKDAVRRP QADPALLTPR SPVVTIMGHV DHGKTTLLDK FRKTQVAAVE TGGITQHIGA FLVSLPSGEK I TFLDTPGH AAFSAMRARG AQVTDIVVLV VAADDGVMKQ TVESIQHAKD AQVPIILAVN KCDKAEADPE KVKKELLAYD VV CEDYGGD VQAVPVSALT GDNLMALAEA TVALAEMLEL KADPNGPVEG TVIESFTDKG RGLVTTAIIQ RGTLRKGSVL VAG KCWAKV RLMFDENGKT IDEAYPSMPV GITGWRDLPS AGEEILEVES EPRAREVVDW RKYEQEQEKG QEDLKIIEEK RKEH KEAHQ KAREKYGHLL WKKRSILRFL ERKEQIPLKP KEKRERDSNV LSVIIKGDVD GSVEAILNII DTYDASHECE LELVH FGVG DVSANDVNLA ETFDGVIYGF NVNAGNVIQQ SAAKKGVKIK LHKIIYRLVE DLQEELSSRL PCAVEEHPVG EASILA TFS VTEGKKKVPV AGCRVQKGQL EKQKKFKLTR NGHVIWKGSL TSLKHHKDDI SIVKTGMDCG LSLDEDNMEF QVGDRIV CY EEKQIQAKTS WDPGF UniProtKB: Translation initiation factor IF-2, mitochondrial

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Macromolecule #15: Mitochondrial ribosomal protein L2

Macromolecule	Name: Mitochondrial ribosomal protein L2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 33.270934 KDa
Sequence	String: MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD ...String: MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD HIGRMAVAAR EGDAHPLGAL PVGTLINNVE SEPGRGAQYI RAAGTCGVLL RKVNGTAIIQ LPSKRQMQVL ET CIATVGR VSNVDHNKRV IGKAGRNRWL GKRPNSGLWQ RKGGWAGRKI RPLPPMKSYV KLPSAAAQS UniProtKB: Mitochondrial ribosomal protein L2

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Macromolecule #16: ICT1

Macromolecule	Name: ICT1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 38.427199 KDa
Sequence	String: MPGWGLLGRA GARVLGCGAD GLGASRGLGN RTDICLLVRS LHGKSVTWWD EHLSEENVPF VKQLVSDENK AQLASKLCPL KDEPWPIHP WEPGSSRVGL IALKLGMMPL WTKDGQKHVV TLLQVQDCHV LKYTPKENHN GRMAALTVGG KTVSHFHKSA S ILEFYQEL ...String: MPGWGLLGRA GARVLGCGAD GLGASRGLGN RTDICLLVRS LHGKSVTWWD EHLSEENVPF VKQLVSDENK AQLASKLCPL KDEPWPIHP WEPGSSRVGL IALKLGMMPL WTKDGQKHVV TLLQVQDCHV LKYTPKENHN GRMAALTVGG KTVSHFHKSA S ILEFYQEL GLPPKQKVKI FNVTENAVIK PGTPLYAAHF RPGQYVDVTA KTIGKGFQGV MRRWGFKGQP ATHGQTKTHR RP GAISTGD VARVWPGTKM PGQLGNIDRT AFGLKVWRIN TKHNIIYVNG SVPGHKNCLV KIKDSKLPAY KDFCKNLPFP TYF PDGDEE ALPEDLYDEN VCQPGAPSIT FT

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Macromolecule #17: Mitochondrial ribosomal protein L4

Macromolecule	Name: Mitochondrial ribosomal protein L4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 33.251469 KDa
Sequence	String: MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK ...String: MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK VRVQGLKVAL TVKLAQDDLH IVDSLELPTA DPQYLIELAR YRRWGDSVLL VDLEHEDMPQ NVVAATSGLK TF NLVPAVG LNVHSMLKHQ TLVLTLPTVA FLEEKLLWHN SRYTPLYPFR LPYCDFP UniProtKB: Large ribosomal subunit protein uL4m

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Macromolecule #18: Mitochondrial ribosomal protein L9

Macromolecule	Name: Mitochondrial ribosomal protein L9 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 30.373449 KDa
Sequence	String: MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR ...String: MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR RCHLEVGMKN NVKWELNPEI VARHFLRNLG VVVAPHALKL PEEPITQRGE YWCEVTVNGL DTVRVPMSVV NF ERPKTKR YKYWLAQQAA KGDVPTSSQM I UniProtKB: Large ribosomal subunit protein bL9m

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Macromolecule #19: Mitochondrial ribosomal protein L10

Macromolecule	Name: Mitochondrial ribosomal protein L10 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 29.449689 KDa
Sequence	String: MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL ...String: MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL KGIPFLPLLG GCIDDTILSR QGFINYSKLP SLALVQGELV GGLTFLTAQT YSMLQHQPRQ LTALLDQYVK QQ QEGHPAV PASGQPEPPD PVPDP UniProtKB: Large ribosomal subunit protein uL10m

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Macromolecule #20: Mitochondrial ribosomal protein L11

Macromolecule	Name: Mitochondrial ribosomal protein L11 / type: protein_or_peptide / ID: 20 Details: Unassigned residues have been built as poly-alanine. Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 20.809271 KDa
Sequence	String: MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ ...String: MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ KERALFLAAQ READLAAQAE AAKK UniProtKB: Large ribosomal subunit protein uL11m

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Macromolecule #21: Mitochondrial ribosomal protein L13

Macromolecule	Name: Mitochondrial ribosomal protein L13 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 20.633801 KDa
Sequence	String: MSSFSKAPQQ WATFARVWYL LDGKMQPPGK LAAMASVKLQ GLHKPVYHQL SDCGDHVVIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQ VTAAQLHQKD PVAIVKLAIY GMLPKNLHRR TMMQRLHLFP DEDIPEDILK NLVEELPQPR KVPRRLDEYT Q EEIEAFPR VWSPPEDYRL

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Macromolecule #22: Mitochondrial ribosomal protein L14

Macromolecule	Name: Mitochondrial ribosomal protein L14 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 15.955632 KDa
Sequence	String: MAFCTGLRGP FAQVSRALSQ RCFSTTGSLS AIQKMTRVRV VDNSALGTTP YHRPPRCIHV YNKTGVGKVG DRILLAIRGQ KKKALIVGH RMPGPRMTPR FDSNNVVLLE DNGNPVGTRI KTPIPSSLRQ REGEFSKVLA IAQNFV

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Macromolecule #23: Mitochondrial ribosomal protein L15

Macromolecule	Name: Mitochondrial ribosomal protein L15 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 33.481723 KDa
Sequence	String: MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE ...String: MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE KNGGVVTTAF YDPRSLEILC KPVPFFLRGQ PIPKRMLPPE ALVPYYTDAK NRGYLADPAR FPEARLELAR KY GYVLPDI TKDELFKMLS TRKDPRQIFF GLAPGWVVNM ADKKILKPTD ENLLKYYSS UniProtKB: 39S ribosomal protein L15, mitochondrial

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Macromolecule #24: Mitochondrial ribosomal protein L16

Macromolecule	Name: Mitochondrial ribosomal protein L16 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 28.680531 KDa
Sequence	String: MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE ...String: MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE FKEVQGFLDL VAHKLPFPAK AVSRETLEKM RKDQEERERN NQNPWTFERI ATANMLGIRK VLSPYDLTHK GR YWGKFYT PERV UniProtKB: Large ribosomal subunit protein uL16m

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Macromolecule #25: Mitochondrial ribosomal protein L17

Macromolecule	Name: Mitochondrial ribosomal protein L17 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 19.268178 KDa
Sequence	String: MRLSVAAAIS HGRVFRRLGL GPESRIHLLQ NLLTGLVRHE RIEASWARVD ELRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKL FQVLAPRFQG QNGGYTRMLQ IPNRNEQDRA KMAVIEYKGN CLPPLPLPRR DSNLTLLNQL LQGLRQDQEA S SHTAQTPA V UniProtKB: Large ribosomal subunit protein bL17m

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Macromolecule #26: Mitochondrial ribosomal protein L18

Macromolecule	Name: Mitochondrial ribosomal protein L18 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 20.582568 KDa
Sequence	String: MALRSRFWAL LSICRNPGCR AAALSTSCKP ATNPETDPVE NEAVAPEFTN RNPRNLELLA VARKERGWGT VWPSREFWHR LRVIRTQHH IEALVEHRNG QVVVSASTRE WAIKKHLYST RNVVACESVG RVLAERCLEA GINFMVYHPT PWEAASDSIK R LQHAMTEG GVVLREPRRI YE UniProtKB: Large ribosomal subunit protein uL18m

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Macromolecule #27: Mitochondrial ribosomal protein L19

Macromolecule	Name: Mitochondrial ribosomal protein L19 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 33.441539 KDa
Sequence	String: MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN ...String: MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN PRIQEIQVVK LEKRLDNSLL YLRDALPEYS TFDVNMKPIT QEFNQEVPVN QLKVKMKPKP WSKRWERPKF NI KGIRFDL CLTEEQMREA QKWSQPWLEF DMMREYDTSK IEAAIWDEIE ASKNS

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Macromolecule #28: Mitochondrial ribosomal protein L20

Macromolecule	Name: Mitochondrial ribosomal protein L20 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 17.554793 KDa
Sequence	String: MVFLTVPLWL RSRVTDRYWR VQEVLKHARH FRGRKNRCYR LAVRAVTRAF VKCTRARRLK KRSLRTLWIN RITAASQEHG LKYPAFIIN LIKCQVELNR KVLADLAIYE PKTFKSLAAL AKRRREEGFA AALGDGKEPD GIFSRVVQHR UniProtKB: Large ribosomal subunit protein bL20m

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Macromolecule #29: Mitochondrial ribosomal protein L21

Macromolecule	Name: Mitochondrial ribosomal protein L21 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 23.154861 KDa
Sequence	String: MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ...String: MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ESWPKINMKF QKRKNYQRKR ITVNPQTVLR INSIEIAPCL C UniProtKB: Large ribosomal subunit protein bL21m

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Macromolecule #30: Mitochondrial ribosomal protein L22

Macromolecule	Name: Mitochondrial ribosomal protein L22 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 24.313357 KDa
Sequence	String: MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV ...String: MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV FCHYFVKLVE GPPPPREAPK TAVAHAREYI QELRNRTITH AL UniProtKB: Large ribosomal subunit protein uL22m

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Macromolecule #31: Mitochondrial ribosomal protein L23

Macromolecule	Name: Mitochondrial ribosomal protein L23 / type: protein_or_peptide / ID: 31 Details: Unassigned residues have been built as poly-alanine. Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 17.643205 KDa
Sequence	String: MARNVLYPLY QLGNPQLRVF RTNFFIQLVR PGTAQPEDTV QFRIPMEMTR VDLRNYLERI YNVPVAAVRT RVQYGSNRRR DHRNIRIKK PDYKVAYVQL ALGQTFTFPD LFPERKGASV DVDVRDQVLE DQRQKHSPDP RRGGVPGWFG L UniProtKB: 39S ribosomal protein L23, mitochondrial

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Macromolecule #32: Mitochondrial ribosomal protein L24

Macromolecule	Name: Mitochondrial ribosomal protein L24 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 24.893529 KDa
Sequence	String: MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE ...String: MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE TWIDGPKDTS VEDALEKTYV PRLKTLEEEV MEAMGIQETR RHKKVYWY UniProtKB: Large ribosomal subunit protein uL24m

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Macromolecule #33: Mitochondrial ribosomal protein L37

Macromolecule	Name: Mitochondrial ribosomal protein L37 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 47.917988 KDa
Sequence	String: MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE ...String: MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE DIPKRETYCP VIVDSLIQLC KSQILKHPSL ARRICAQNNT LSATWNRESI LLQVHGSSGA RLNAKDPLPP VA SQEEVEA TKNHVLETFY PISPTMGLQE CNVYDVNDDT GFQEGYPYPC PHTLYFLESA NLRPRRFQPD QLRAKMILFA FGS ALAQAR LLYGNDSKVL EQPVVVQSVG TDGRLFQFLV LQLNTTDLAS DEGVKNLAWV DSDQLLYQHF WCLPVIKKKV VVEP VGPIG FQPETFRKFL ALYLHGAV UniProtKB: Large ribosomal subunit protein mL37

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Macromolecule #34: Mitochondrial ribosomal protein L38

Macromolecule	Name: Mitochondrial ribosomal protein L38 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 44.656387 KDa
Sequence	String: MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV ...String: MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV PRVPLHVAYA VGEDDLMPVY HGNEVTPTEA AQAPEVTYEA DEGSLWTLLL TNLDGHLLEP DAEYVHWLVT NI PGNRVTE GQETCPYLPP FPARGSGFHR FAFLLFKQDK RIDFSGDTRP SPCYQLAQRT FHTFDFYKKH QDAMTPAGLA FFQ CRWDDS VTRVFHQLLD MREPVFEFVR PPPYHPKQKR FPHRQPLRYL DRYRDSHEPT YGIY UniProtKB: Large ribosomal subunit protein mL38

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Macromolecule #35: Mitochondrial ribosomal protein L39

Macromolecule	Name: Mitochondrial ribosomal protein L39 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 38.523422 KDa
Sequence	String: MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE ...String: MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE VPVIAGAFCY DVVLDKRLDE WMPTKENLHS FTKDARALIY KDLPFETLEV EAKVALEIFQ HNKYKLDFIE EK ASQNPER IVKLHRFGDF IDVSEGPLIP RTSICFQYEV SAVHNLQTQS SLVRRFQGLS LPVHLRAHFT IWNKLLERSR KMV TEDKTK PTEESAST UniProtKB: Large ribosomal subunit protein mL39

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Macromolecule #36: Mitochondrial ribosomal protein L40

Macromolecule	Name: Mitochondrial ribosomal protein L40 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 24.075658 KDa
Sequence	String: MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL ...String: MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL FPFERQGPDY TPPISDYQPP EGRYQDITKV YTQVEFKK UniProtKB: Large ribosomal subunit protein mL40

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Macromolecule #37: Mitochondrial ribosomal protein L41

Macromolecule	Name: Mitochondrial ribosomal protein L41 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 15.073404 KDa
Sequence	String: MGLLSGAARA LVRGADRMSK WTSKRGPRTF CKGRGAKGTG FHGRDGKFVQ IKEMIPELVV PELAGFKLKP YVNYRAPEGT DTPLTAKQL FLETAAPAIE KDFKAGTFDP EHLEKYGFEP TQEGKLFQLY PKNFPR UniProtKB: Mitochondrial ribosomal protein L41

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Macromolecule #38: Mitochondrial ribosomal protein L42

Macromolecule	Name: Mitochondrial ribosomal protein L42 / type: protein_or_peptide / ID: 38 Details: Unassigned residues have been built as poly-alanine. Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 16.680178 KDa
Sequence	String: MALAAVKWVI SSRTILKHLF PIQNSASYCV CHKSTYSSLP DDYNCKVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNIEETHD LVLKTRLEEK GEHLEQGPMI EQLSKMFFTT KHRWYPRGQY HRRRRKPNPP KDR UniProtKB: Large ribosomal subunit protein mL42

+
Macromolecule #39: Mitochondrial ribosomal protein L43

Macromolecule	Name: Mitochondrial ribosomal protein L43 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 17.728109 KDa
Sequence	String: MTARGTASRF LTSVLHNGLG RYVQQLQRLS FSLSRDAPSS RGAREFVERE VTDFARRNPG VVIYVNPRPC CVPRVVAEYL NGAVREESI HCKSVEEIAA LVQKLADQSG LDVIRIRKPF HTDSPSIQGQ WHPFTNKPTT LGGLRPREVQ DPAPAQVQAQ UniProtKB: Large ribosomal subunit protein mL43

+
Macromolecule #40: Mitochondrial ribosomal protein L44

Macromolecule	Name: Mitochondrial ribosomal protein L44 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 37.527195 KDa
Sequence	String: MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH ...String: MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH VARNLAVEQL ALSAEFPVPP PVLRQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWTITNPM GL LVEELKK RKISAPESRL TRQSGSTTAL PVYFVGLYCD RKLIAEGPGE TVLVAEEEAA RVALRKLFGF TENRRPWDYS KPK EHVRAE KTITAS UniProtKB: Large ribosomal subunit protein mL44

+
Macromolecule #41: Mitochondrial ribosomal protein L45

Macromolecule	Name: Mitochondrial ribosomal protein L45 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 35.331805 KDa
Sequence	String: MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC ...String: MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC FPDMVWDIRY KTVRWSFVES LEPPQVVQVR CSSLMNQGNI YGQVTVRMHT RQTLAIYDRF GRLMYGQEDV PR DVLEYVV FEKHLVDPYG SWRMHGKIIP PWAPPKQPIL KTVMIPGPQL KPWEEFEEPQ GEVHKPQPA UniProtKB: Large ribosomal subunit protein mL45

+
Macromolecule #42: Mitochondrial ribosomal protein L46

Macromolecule	Name: Mitochondrial ribosomal protein L46 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 32.215998 KDa
Sequence	String: MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ ...String: MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ DIWMLPQSDW QPGETLRQTA ERTLATLSEN NMEAKFLGNA PCGHYKFKFP QAMRTETSLG AKVFFFKALL LT GDFSQAG KKGHHVWVSK EELGDYLKPK YLAQVRRFLL DL UniProtKB: Large ribosomal subunit protein mL46

+
Macromolecule #43: Mitochondrial ribosomal protein L48

Macromolecule	Name: Mitochondrial ribosomal protein L48 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 23.749512 KDa
Sequence	String: MNGALGKALC LRNDTVLKQA LSLIRVRASG ESPICSAGGI LLSTSRHYRS KPTHGIGRYK HLVKAQEPKK KKGKVEVRPI NLGTDYEYG VLNIHLIAYD MALAESYAQY VHNLCNHLAI KVEESYAMPT KTMEVLQLQE QGSKMFLDAV LTTHERVVQI S GLSATFAE ...String: MNGALGKALC LRNDTVLKQA LSLIRVRASG ESPICSAGGI LLSTSRHYRS KPTHGIGRYK HLVKAQEPKK KKGKVEVRPI NLGTDYEYG VLNIHLIAYD MALAESYAQY VHNLCNHLAI KVEESYAMPT KTMEVLQLQE QGSKMFLDAV LTTHERVVQI S GLSATFAE IFLEIIHSNL PEGVKLSVRE HTEEDFKGRF KARPELEELL AKLN UniProtKB: Large ribosomal subunit protein mL48

+
Macromolecule #44: Mrpl34

Macromolecule	Name: Mrpl34 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 18.957779 KDa
Sequence	String: MAATVLCGVL RAWRTGVPLG CGLRRLSQTQ GTPEYPSFVE SVDEYHFVER LLPPASIPRP PKHEHYPTPS GWQPPRDPAP SLPYFVRRS RMHNIPVYRD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRV KGYFDQQLKA W LLEKGF UniProtKB: Large ribosomal subunit protein mL49

+
Macromolecule #45: Mitochondrial ribosomal protein L50

Macromolecule	Name: Mitochondrial ribosomal protein L50 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 18.351 KDa
Sequence	String: MAARWVSGLA RRSLTCAVSG APRREFWSPF RKEKQPVVAE TVEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQD ISLEDVHLKF SFLARLANDL RHAVPNSRLH QMCRVRDVLD FYNVPVQDRS KFDELIASNL PHNLKITWGY UniProtKB: Large ribosomal subunit protein mL50

+
Macromolecule #46: Mitochondrial ribosomal protein L51

Macromolecule	Name: Mitochondrial ribosomal protein L51 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 15.249048 KDa
Sequence	String: MAGSLSWVTG RGLWGRLPLT CRSFSLGIPR LFHVRVTLPP RKVVDRWNEK RAMFGVYDNI GILGNFEKHP KELIKGPIWL RGWKGNELQ RCIRKKRMVG NRMFIDDLHN LNKRISYLYK HFNRHGKYR UniProtKB: Large ribosomal subunit protein mL51

+
Macromolecule #47: Mitochondrial ribosomal protein L52

Macromolecule	Name: Mitochondrial ribosomal protein L52 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 13.813705 KDa
Sequence	String: MAAWGILLST GVRRLHCGTA AQAGSQWRLQ QGFAANPSGY GPLTELPDWS YADGRPAPPM KGQLRRKAQR EKFARRVVLL SQEMDAGLQ AWQLRQQEKL QEEEGKQKNA LKSKGALLQN PQPSQ UniProtKB: Large ribosomal subunit protein mL52

+
Macromolecule #48: mL53, MRPL53

Macromolecule	Name: mL53, MRPL53 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 12.015747 KDa
Sequence	String: MAAALARLGL RAVKQVRVQF CPFEKNVEST RTFLQAVSSE KVRCTNLNCS VIADVRHDGS EPCVDVLFGD GHRLIMRGAH LTAQEMLTA FASHIQARGA AGSGDKPSAS TGR

+
Macromolecule #49: Uncharacterized protein

Macromolecule	Name: Uncharacterized protein / type: protein_or_peptide / ID: 49 Details: Unassigned residues have been built as poly-alanine. Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 15.957357 KDa
Sequence	String: MAARRLFGAA RSWAAWRAWE LSDAAVSGRL HVRNYAKRPV IKGGKGGKGA VVGEALKDPE VCTDPFRLTT HAMGVNIYKE GQDVVLKPD SEYPEWLFEM NVGPPKKLEE LDPETREYWR LLRKHNIWRH NRLSKNRKF UniProtKB: Large ribosomal subunit protein mL54

+
Macromolecule #50: Mitochondrial ribosomal protein L57

Macromolecule	Name: Mitochondrial ribosomal protein L57 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 12.001915 KDa
Sequence	String: MFLTALLRRN RIPGRQWIGK HRRPRPVSAQ AKQNMIRRLE TEAENQYWLS RPFLTAEQER GHAAVRRAAA FQALKAAQAA RFPAHRRLE EQLGHLLVTR KWS UniProtKB: Mitochondrial ribosomal protein L57

+
Macromolecule #51: Mitochondrial ribosomal protein L58

Macromolecule	Name: Mitochondrial ribosomal protein L58 / type: protein_or_peptide / ID: 51 Details: Unassigned residues have been built as poly-alanine. Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 23.545873 KDa
Sequence	String: MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS ...String: MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS REDAELRRSR IENMNRERLR KKRISSAIKT SRRVDVD UniProtKB: Large ribosomal subunit protein mL62

+
Macromolecule #52: 'Mitochondrial ribosomal protein L59

Macromolecule	Name: 'Mitochondrial ribosomal protein L59 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 25.815031 KDa
Sequence	String: MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV ...String: MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV DPRSARFQEL LQDMEKQHRK RLKEEKQRKK KEARAAAMAA AAAQDPADSE TPSS UniProtKB: Large ribosomal subunit protein mL64

+
Macromolecule #53: mL65, MRPS30

Macromolecule	Name: mL65, MRPS30 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 49.100504 KDa
Sequence	String: MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS ...String: MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS PFLDQLVASL TGLLSVHNPV LAAAALDCKR PVHFFWLRGE EIIPRGHRKG RVDALRYQIN DKPHNQIRIS RQ LPEFVPL DYSIPIEVPV MSCKPDKLPL FKRQYENTIF IGSKTADPLC YGHTQFHLLP DKLKREKLLK QNCADQIEVV FRA NAIASL FAWTGAQAMY QGFWSEADVT RPFVSQGVIT DGKYFSFFCY QLNTLALTAQ ADQNNPRKNI CWGTQSKPLY ETIE DNNVK GFNDDVLLQL VQFLLNRPKE DKSQLLEN

+
Macromolecule #54: Mitochondrial ribosomal protein S18A

Macromolecule	Name: Mitochondrial ribosomal protein S18A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 22.273084 KDa
Sequence	String: MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR ...String: MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR WNKVRMPVGS PLLKDNVSYS GRPLLLYH UniProtKB: Large ribosomal subunit protein mL66

+
Macromolecule #55: unassigned secondary structure elements

Macromolecule	Name: unassigned secondary structure elements / type: protein_or_peptide / ID: 55 Details: Unassigned secondary structure elements have been built as poly-alanine. Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sus scrofa (pig) / Organ: liver
Molecular weight	Theoretical: 5.846387 KDa
Sequence	String: AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA

+
Macromolecule #12: 16S ribosomal RNA, mitochondrial

Macromolecule	Name: 16S ribosomal RNA, mitochondrial / type: rna / ID: 12 / Number of copies: 1
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 504.852031 KDa
Sequence	String: ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA ...String: ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA UUACCCCUUC UACCUUUUGC AUAAUGGUUU AACCAGAAAA AAUCUAACAA AGAGAACUUU AGCUAGAUAC CC CGAAACC AGACGAGCUA CCCAUGAGCA GUUUAAAAGA ACCAACUCAU CUAUGUGGCA AAAUAGUGAG AAGACUUGUA GGU AGAGGU GAAAAGCCUA ACGAGCCUGG UGAUAGCUGG UUGUCCGAGA AAGAAUUUUA GUUCAACCUU AAAAAUACCC CAAA AACCC UAAAUUCCAA UGUAUUUUUA AGAGAUAGUC UAAAAAGGUA CAGCUUUUUA GAAACGGAUA CAACCUUGAC UAGAG AGUA AAAUCUUAAU ACUACCAUAG UAGGCCUAAA AGCAGCCAUC AAUUGAGAAA GCGUUAAAGC UCAACAAAUU CACCAA CAU AAUCCCAAAA ACUAAUAACA AACUCCUAGC CCAAUACCGG ACUAAUCUAU UGAAACAUAG AAGCAAUAAU GUUAAUA UG AGUAACAAGA AGCCUUUCUC CUCGCACACG CUUACAUCAG UAACUAAUAA UAUACUGAUA AUUAACAACC AAUAAACC A AAACAACACU AAAACGUUUA UUAAUUACAU UGUUAACCCA ACACAGGAGU GCACCAAGGA AAGAUUAAAA GAAGUAAAA GGAACUCGGC AAACACAAAC CCCGCCUGUU UACCAAAAAC AUCACCUCUA GCAUUACUAG UAUUAGAGGC AAUGCCUGCC CAGUGACAC CAGUUUAACG GCCGCGGUAU UCUGACCGUG CAAAGGUAGC AUAAUCACUU GUUCUCCAAA UAAGGACUUG U AUGAAUGG CCACACGAGG GUUUUACUGU CUCUUACUUC CAAUCAGUGA AAUUAACCUU CCCGUGAAGA GGCGGGAAUA AA AAAAUAA GACGAGAAGA CCCUAUGGAG CUUUAAUUAA CUAUUCCAAA AGUUAAACAA CUCAACCACA AAGGGAUAAA ACA UAACUU AACAUGGACU AGCAAUUUCG GUUGGGGUGA CCUCGGAGUA CAAAAAACCC UCCGAGUGAU UUUAAUCUAG ACAA ACCAG UCAAAAUAAC CAUAACAUCA CUUAUUGAUC CAAAAUUUUG AUCAACGGAA CAAGUUACCC UAGGGAUAAC AGCGC AAUC CUGUUCUAGA GUUCCUAUCG ACAAUAGGGU UUACGACCUC GAUGUUGGAU CAGGACACCC AAAUGGUGCA ACCGCU AUU AAAGGUUCGU UUGUUCAACG AUUAAAGUCC UACGUGAUCU GAGUUCAGAC CGGAGCAAUC CAGGUCGGUU UCUAUCU AU UAUAAAUUUC UCCCAGUACG AAAGGACAAG AGAAAUGGGA CCAACCUCAC AAACGCGUCU CAGAGAUAAU UAAUGAUU U AAUCUUAACC UAAUUAACUC AUAAUAAAUC CAGCCCUAGA ACAGGGCACA GENBANK: GENBANK: AF486858.1

+
Macromolecule #13: CP tRNAPhe, mitochondrial

Macromolecule	Name: CP tRNAPhe, mitochondrial / type: rna / ID: 13 / Number of copies: 1
Source (natural)	Organism: Sus scrofa (pig)
Molecular weight	Theoretical: 23.402018 KDa
Sequence	String: GUUAAUGUAG CUUAAAUUAU CAAAGCAAGG CACUGAAAAU GCCUAGAUGA GCCUCACAGC UCCAUAAACA CCA GENBANK: GENBANK: DQ207753.1

+
Macromolecule #56: P-site fMet-tRNAMet, mitochondrial

Macromolecule	Name: P-site fMet-tRNAMet, mitochondrial / type: rna / ID: 56 / Number of copies: 1
Source (natural)	Organism: Homo sapiens (human)
Molecular weight	Theoretical: 22.664498 KDa
Sequence	String: AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A GENBANK: GENBANK: KY671085.1

+
Macromolecule #57: MAGNESIUM ION

+
Macromolecule #58: ZINC ION

+
Macromolecule #59: GUANOSINE-5'-MONOPHOSPHATE

Macromolecule	Name: GUANOSINE-5'-MONOPHOSPHATE / type: ligand / ID: 59 / Number of copies: 2 / Formula: 5GP
Molecular weight	Theoretical: 363.221 Da
Chemical component information	ChemComp-5GP: GUANOSINE-5'-MONOPHOSPHATE

+
Macromolecule #60: SPERMINE

+
Macromolecule #61: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

Macromolecule	Name: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / type: ligand / ID: 61 / Number of copies: 1 / Formula: GSP
Molecular weight	Theoretical: 539.246 Da
Chemical component information	ChemComp-GSP: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

+
Macromolecule #62: SODIUM ION

+
Macromolecule #63: N-FORMYLMETHIONINE

Macromolecule	Name: N-FORMYLMETHIONINE / type: ligand / ID: 63 / Number of copies: 1 / Formula: FME
Molecular weight	Theoretical: 177.221 Da
Chemical component information	ChemComp-FME: N-FORMYLMETHIONINE

+
Macromolecule #64: water

-
Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

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Sample preparation

Concentration	0.171 mg/mL
Buffer	pH: 7.6
Grid	Model: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
Vitrification	Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
Details	contains 55S mitochondrial ribosome, mitochondrial initiation factor 2, mitochondrial formyl-Met-tRNAMet and MT-CO3 mRNA

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Electron microscopy

Microscope	FEI TITAN KRIOS
Image recording	Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number real images: 13936 / Average exposure time: 1.4 sec. / Average electron dose: 40.0 e/Å²
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selection	Number selected: 1366787
Startup model	Type of model: PDB ENTRY PDB model - PDB ID: 5aj4
Final reconstruction	Number classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 75666
Initial angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)

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Atomic model buiding 1

Refinement	Space: RECIPROCAL / Protocol: OTHER / Overall B value: 56.9
Output model	PDB-6gb2: Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit.

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Macromolecule	Name: MAGNESIUM ION / type: ligand / ID: 57 / Number of copies: 216 / Formula: MG
Molecular weight	Theoretical: 24.305 Da

Macromolecule	Name: ZINC ION / type: ligand / ID: 58 / Number of copies: 3 / Formula: ZN
Molecular weight	Theoretical: 65.409 Da

Macromolecule	Name: SPERMINE / type: ligand / ID: 60 / Number of copies: 2 / Formula: SPM
Molecular weight	Theoretical: 202.34 Da
Chemical component information	ChemComp-SPM: SPERMINE

Macromolecule	Name: SODIUM ION / type: ligand / ID: 62 / Number of copies: 1
Molecular weight	Theoretical: 22.99 Da

Macromolecule	Name: water / type: ligand / ID: 64 / Number of copies: 2 / Formula: HOH
Molecular weight	Theoretical: 18.015 Da
Chemical component information	ChemComp-HOH: WATER

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-Sample components

+Entire : mammalian mitochondrial translation initiation complex

+Supramolecule #1: mammalian mitochondrial translation initiation complex

+Supramolecule #2: mammalian mitochondrial ribosome

+Supramolecule #3: Translation initiation factor IF-2, mitochondrial

+Supramolecule #4: tRNA

+Macromolecule #1: Mitochondrial ribosomal protein L12

+Macromolecule #2: Mitochondrial ribosomal protein L27

+Macromolecule #3: Mitochondrial ribosomal protein L28

+Macromolecule #4: Mitochondrial ribosomal protein L47

+Macromolecule #5: 'Mitochondrial ribosomal protein L30

+Macromolecule #6: 'Mitochondrial ribosomal protein L55

+Macromolecule #7: Mitochondrial ribosomal protein L32

+Macromolecule #8: Mitochondrial ribosomal protein L33

+Macromolecule #9: Mitochondrial ribosomal protein L34

+Macromolecule #10: Mitochondrial ribosomal protein L35

+Macromolecule #11: Ribosomal protein

+Macromolecule #14: Translation initiation factor IF-2, mitochondrial

+Macromolecule #15: Mitochondrial ribosomal protein L2

+Macromolecule #16: ICT1

+Macromolecule #17: Mitochondrial ribosomal protein L4

+Macromolecule #18: Mitochondrial ribosomal protein L9

+Macromolecule #19: Mitochondrial ribosomal protein L10

+Macromolecule #20: Mitochondrial ribosomal protein L11

+Macromolecule #21: Mitochondrial ribosomal protein L13

+Macromolecule #22: Mitochondrial ribosomal protein L14

+Macromolecule #23: Mitochondrial ribosomal protein L15

+Macromolecule #24: Mitochondrial ribosomal protein L16

+Macromolecule #25: Mitochondrial ribosomal protein L17

+Macromolecule #26: Mitochondrial ribosomal protein L18

+Macromolecule #27: Mitochondrial ribosomal protein L19

+Macromolecule #28: Mitochondrial ribosomal protein L20

+Macromolecule #29: Mitochondrial ribosomal protein L21

+Macromolecule #30: Mitochondrial ribosomal protein L22

+Macromolecule #31: Mitochondrial ribosomal protein L23

+Macromolecule #32: Mitochondrial ribosomal protein L24

+Macromolecule #33: Mitochondrial ribosomal protein L37

+Macromolecule #34: Mitochondrial ribosomal protein L38

+Macromolecule #35: Mitochondrial ribosomal protein L39

+Macromolecule #36: Mitochondrial ribosomal protein L40

+Macromolecule #37: Mitochondrial ribosomal protein L41

+Macromolecule #38: Mitochondrial ribosomal protein L42

+Macromolecule #39: Mitochondrial ribosomal protein L43

+Macromolecule #40: Mitochondrial ribosomal protein L44

+Macromolecule #41: Mitochondrial ribosomal protein L45

+Macromolecule #42: Mitochondrial ribosomal protein L46

+Macromolecule #43: Mitochondrial ribosomal protein L48

+Macromolecule #44: Mrpl34

+Macromolecule #45: Mitochondrial ribosomal protein L50

+Macromolecule #46: Mitochondrial ribosomal protein L51

+Macromolecule #47: Mitochondrial ribosomal protein L52

+Macromolecule #48: mL53, MRPL53

+Macromolecule #49: Uncharacterized protein

+Macromolecule #50: Mitochondrial ribosomal protein L57

+Macromolecule #51: Mitochondrial ribosomal protein L58

+Macromolecule #52: 'Mitochondrial ribosomal protein L59

+Macromolecule #53: mL65, MRPS30

+Macromolecule #54: Mitochondrial ribosomal protein S18A

+Macromolecule #55: unassigned secondary structure elements

+Macromolecule #12: 16S ribosomal RNA, mitochondrial

+Macromolecule #13: CP tRNAPhe, mitochondrial

+Macromolecule #56: P-site fMet-tRNAMet, mitochondrial

+Macromolecule #57: MAGNESIUM ION

+Macromolecule #58: ZINC ION

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