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Entry
Database: EMDB / ID: 4369
TitleUnique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit.
Map data
Samplemammalian mitochondrial translation initiation complex
  • Porcine Ribosome
  • (Translation initiation factor IF-2, ...) x 2
  • Nucleic acidsNucleic acid
  • (Mitochondrial ribosomal protein ...) x 25
  • (nucleic-acidNucleic acid) x 3
  • bS16m, MRPS16
  • unassigned secondary structure elements
  • Mitoribosomal protein ms28, mrps28
  • Death associated protein 3
  • mS31, MRPS31
  • Aurora kinase A interacting protein 1
  • 28S ribosomal protein S18b, mitochondrialRibosome
  • (ligand) x 8
Function / homologyCoiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Ribosomal protein S23/S25, mitochondrial / Ribosomal protein S18 superfamily / Ribosomal protein S10 domain superfamily / Ribosomal protein S7 domain superfamily / Thioredoxin-like superfamily / Ribosomal protein S6 superfamily / MRPS27/PTCD2 / Mitochondrial ribosome subunit S26 / Mitochondrial 28S ribosomal protein S34 ...Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Ribosomal protein S23/S25, mitochondrial / Ribosomal protein S18 superfamily / Ribosomal protein S10 domain superfamily / Ribosomal protein S7 domain superfamily / Thioredoxin-like superfamily / Ribosomal protein S6 superfamily / MRPS27/PTCD2 / Mitochondrial ribosome subunit S26 / Mitochondrial 28S ribosomal protein S34 / Ribosomal protein S10 domain / P-loop containing nucleoside triphosphate hydrolase / 28S ribosomal protein S24, mitochondrial / 28S ribosomal protein S26 / Ribosomal protein S7 domain / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S11 superfamily / Translation initiation factor IF- 2, domain 3 / Ribosomal protein S9, conserved site / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S23/Rsm25, mitochondrial / Ribosomal protein S28, mitochondrial / Ribosomal protein S22, mitochondrial / Ribosomal protein S23/S29, mitochondrial / Ribosomal protein S24/S35, mitochondrial, conserved domain / 28S ribosomal protein S27, mitochondrial / Ribosomal protein S18, conserved site / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S2, conserved site / Ribosomal S11, conserved site / Translation initiation factor IF-2, domain 3 superfamily / Pentatricopeptide repeat domain-containing protein 3 / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein S21 / Mitochondrial ribosomal protein S23 / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal death-associated protein 3 / Mitochondrial ribosomal subunit protein / Mitochondrial 28S ribosomal protein S27 / Mitochondrial ribosomal subunit S27 / Mitochondrial domain of unknown function (DUF1713) / CHCH domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S5, C-terminal domain / Ribosomal protein S6 / Ribosomal protein L19 / Ribosomal protein S18 / Ribosomal protein S21 superfamily / Ribosomal protein S11 / Ribosomal protein S9/S16 / Ribosomal protein S17 / Ribosomal protein S10p/S20e / Ribosomal protein S5, N-terminal domain / Ribosomal protein S2 / Ribosomal protein S15 / Ribosomal protein S14p/S29e / Ribosomal protein S7p/S5e / Ribosomal protein S12/S23 / Elongation factor Tu GTP binding domain / 28S ribosomal protein S17, mitochondrial / Ribosomal protein L19 superfamily / Translation initiation factor IF- 2 / Translation elongation factor EF1B/ribosomal protein S6 / Pentatricopeptide repeat domain / Ribosomal protein S12 signature. / Ribosomal protein S9 / Ribosomal protein S15 / Ribosomal protein S6 / Ribosomal protein S5, N-terminal / Ribosomal protein S5/S7 / Translation initiation factor aIF-2, bacterial-like / Mitochondrial ribosome subunit S24 / Mitochondrial 28S ribosomal protein S34 / Ribosomal protein S11 signature. / Ribosomal protein S18 signature. / Ribosomal protein S5 / Ribosomal protein S9 signature. / Ribosomal protein S5 signature. / Ribosomal protein S2 signature 1. / Initiation factor 2 signature. / S5 double stranded RNA-binding domain profile. / Pentatricopeptide (PPR) repeat profile. / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Mitochondrial translation initiation / Mitochondrial translation elongation / Mitochondrial translation termination / Transcription factor, GTP-binding domain / Ribosomal protein S17/S11 / Ribosomal protein S14 / Ribosomal protein S12, bacterial-type / Ribosomal protein S27/S33, mitochondrial / Domain of unknown function DUF1713 / Nucleic acid-binding, OB-fold / Tetratricopeptide-like helical domain superfamily / CHCH / Cysteine alpha-hairpin motif superfamily / S15/NS1, RNA-binding / Translation protein, beta-barrel domain superfamily / Translation protein SH3-like domain superfamily
Function and homology information
SourceSus scrofa (pig) / Homo sapiens (human) / Pig (pig)
Methodsingle particle reconstruction / cryo EM / 3.1 Å resolution
AuthorsKummer E / Leibundgut M / Boehringer D / Ban N
CitationJournal: Nature / Year: 2018
Title: Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM
Authors: Kummer E / Leibundgut M / Rackham O / Lee RG / Boehringer D / Filipovska A / Ban N
Validation ReportPDB-ID: 6gaz

SummaryFull reportAbout validation report
DateDeposition: Apr 13, 2018 / Header (metadata) release: May 9, 2018 / Map release: Aug 8, 2018 / Last update: Aug 8, 2018

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.085
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.085
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6gaz
  • Surface level: 0.085
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_4369.map.gz (map file in CCP4 format, 88753 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
281 pix
1.39 Å/pix.
= 390.59 Å
281 pix
1.39 Å/pix.
= 390.59 Å
281 pix
1.39 Å/pix.
= 390.59 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.39 Å
Density
Contour Level:0.085 (by author), 0.085 (movie #1):
Minimum - Maximum-0.24249981 - 0.60988814
Average (Standard dev.)0.0011413419 (0.017309353)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions281281281
Origin0.0.0.
Limit280.280.280.
Spacing281281281
CellA=B=C: 390.59 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z281281281
origin x/y/z0.0000.0000.000
length x/y/z390.590390.590390.590
α/β/γ90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS281281281
D min/max/mean-0.2420.6100.001

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Supplemental data

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Mask #1

Fileemd_4369_msk_1.map
Projections & Slices
AxesZYX
Projections
Slices (1/2)
Density Histograms

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Sample components

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Entire mammalian mitochondrial translation initiation complex

EntireName: mammalian mitochondrial translation initiation complex
Number of components: 48

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Component #1: protein, mammalian mitochondrial translation initiation complex

ProteinName: mammalian mitochondrial translation initiation complex
Recombinant expression: No
MassTheoretical: 2.85 MDa

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Component #2: protein, Porcine Ribosome

ProteinName: Porcine Ribosome / Recombinant expression: No
SourceSpecies: Sus scrofa (pig)

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Component #3: protein, Translation initiation factor IF-2, mitochondrial

ProteinName: Translation initiation factor IF-2, mitochondrial / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #4: protein, Nucleic acids

ProteinName: Nucleic acidsNucleic acid / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: synthetic construct (others)

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Component #5: protein, Translation initiation factor IF-2, mitochondrial

ProteinName: Translation initiation factor IF-2, mitochondrial
Details: Unassigned residues have been built as poly-alanine.
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 72.811219 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #6: protein, Mitochondrial ribosomal protein L19

ProteinName: Mitochondrial ribosomal protein L19
Details: Unassigned residues have been built as poly-alanine.
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.441539 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #7: nucleic-acid, 12S ribosomal RNA, mitochondrial

Nucleic-acidName: 12S ribosomal RNA, mitochondrial / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
ACACAGGUUU GGUCCUGGCC UUUCUAUUAA UUCUUAAUAA AAUUACACAU GCAAGUAUCC GCGCCCCGGU GAGAAUGCCC UCCAGAUCUU AAAGAUCAAA AGGAGCAGGU AUCAAGCACA CCUAUAACGG UAGCUCAUAA CGCCUUGCUC AACCACACCC CCACGGGAAA CAGCAGUGAU AAAAAUUAAG CCAUGAACGA AAGUUUGACU AAGUUAUAUU AAUUAGAGUU GGUAAAUCUC GUGCCAGCCA CCGCGGUCAU ACGAUUAACC CAAAUUAAUA GAUCCACGGC GUAAAGAGUG UUUAAGAAAA AAAAUCACAA UAGAGUUAAA UUAUAACUAA GCUGUAAAAA GCCCUAGUUA AAAUAAAAUA ACCCACGAAA GUGACUCUAA UAAUCCUGAC ACACGAUAGC UAGGACCCAA ACUGGGAUUA GAUACCCCAC UAUGCCUAGC CCUAAACCCA AAUAGUUACA UAACAAAACU AUUCGCCAGA GUACUACUCG CAACUGCCUA AAACUCAAAG GACUUGGCGG UGCUUCACAU CCACCUAGAG GAGCCUGUUC UAUAAUCGAU AAACCCCGAU AGACCUUACC AACCCUUGCC AAUUCAGCCU AUAUACCGCC AUCUUCAGCA AACCCUAAAA AGGAACAAUA GUAAGCACAA UCAUAGCACA UAAAAACGUU AGGUCAAGGU GUAGCUUAUG GGUUGGAAAG AAAUGGGCUA CAUUUUCUAC AUAAGAAUAU CCACCACACG AAAGUUUUUA UGAAACUAAA AACCAAAGGA GGAUUUAGCA GUAAAUCAAG AAUAGAGUGC UUGAUUGAAU AAGGCCAUGA AGCACGCACA CACCGCCCGU CACCCUCCUC AAGCAUGUAG UAAUAAAAAU AACCUAUAUU CAAUUACACA ACCAUGCAAG AAGAGACAAG UCGUAACAAG GUAAGCAUAC UGGAAAGUGU GCUUGGAUUA CC
MassTheoretical: 308.989062 kDa
SourceSpecies: Sus scrofa (pig)

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Component #8: protein, Mitochondrial ribosomal protein S2

ProteinName: Mitochondrial ribosomal protein S2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.731604 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #9: protein, Mitochondrial ribosomal protein S24

ProteinName: Mitochondrial ribosomal protein S24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.935172 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #10: protein, Mitochondrial ribosomal protein S5

ProteinName: Mitochondrial ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 48.213074 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #11: protein, Mitochondrial ribosomal protein S6

ProteinName: Mitochondrial ribosomal protein S6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.283703 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #12: protein, Mitochondrial ribosomal protein S7

ProteinName: Mitochondrial ribosomal protein S7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.150918 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #13: protein, Mitochondrial ribosomal protein S9

ProteinName: Mitochondrial ribosomal protein S9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 45.60457 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #14: protein, Mitochondrial ribosomal protein S10

ProteinName: Mitochondrial ribosomal protein S10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.192758 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #15: protein, Mitochondrial ribosomal protein S11

ProteinName: Mitochondrial ribosomal protein S11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.841922 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #16: protein, Mitochondrial ribosomal protein S12

ProteinName: Mitochondrial ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.257107 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #17: protein, Mitochondrial ribosomal protein S14

ProteinName: Mitochondrial ribosomal protein S14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.120739 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #18: protein, Mitochondrial ribosomal protein S15

ProteinName: Mitochondrial ribosomal protein S15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.411428 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #19: protein, bS16m, MRPS16

ProteinName: bS16m, MRPS16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.182668 kDa
SourceSpecies: Sus scrofa (pig)
Source (natural)Organ or tissue: liver

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Component #20: protein, Mitochondrial ribosomal protein S17

ProteinName: Mitochondrial ribosomal protein S17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.452014 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #21: protein, Mitochondrial ribosomal protein S18C

ProteinName: Mitochondrial ribosomal protein S18C / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.211086 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #22: protein, Mitochondrial ribosomal protein S21

ProteinName: Mitochondrial ribosomal protein S21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.682506 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #23: nucleic-acid, P-site fMet-tRNAMet, mitochondrial

Nucleic-acidName: P-site fMet-tRNAMet, mitochondrial / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A
MassTheoretical: 22.664498 kDa
SourceSpecies: Homo sapiens (human)

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Component #24: nucleic-acid, MT-CO3 mRNA, mitochondrial

Nucleic-acidName: MT-CO3 mRNA, mitochondrial / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AUGACCCACC AAUCACAUGC CUAUCAUAUA GUAAAACCCA GCCCAUGACC CCUAACAGGG GCCCUCUCAG CCCUCCUAAU GACCUCCGGC CUAGCCAUGU GAUUUCACUU CCACUCCAUA ACGCUCCUCA UACUAGGCCU ACUAACCAAC ACACUAACCA UAUACCAAUG AUGGCGCGAU GUAACACGAG AAAGCACAUA C
MassTheoretical: 63.946098 kDa
SourceSpecies: Homo sapiens (human)

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Component #25: protein, unassigned secondary structure elements

ProteinName: unassigned secondary structure elements
Details: Unassigned secondary structure elements have been built as poly-alanine.
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 1.297416 kDa
SourceSpecies: Sus scrofa (pig)
Source (natural)Organ or tissue: liver

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Component #26: protein, Mitochondrial ribosomal protein S22

ProteinName: Mitochondrial ribosomal protein S22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 43.234645 kDa
SourceSpecies: Pig (pig)

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Component #27: protein, Mitochondrial ribosomal protein S23

ProteinName: Mitochondrial ribosomal protein S23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.666674 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #28: protein, Mitochondrial ribosomal protein S25

ProteinName: Mitochondrial ribosomal protein S25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.954186 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #29: protein, Mitochondrial ribosomal protein S26

ProteinName: Mitochondrial ribosomal protein S26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.6379 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #30: protein, Mitochondrial ribosomal protein S27

ProteinName: Mitochondrial ribosomal protein S27
Details: Unassigned residues have been built as poly-alanine.
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 45.244129 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #31: protein, Mitoribosomal protein ms28, mrps28

ProteinName: Mitoribosomal protein ms28, mrps28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.014912 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #32: protein, Death associated protein 3

ProteinName: Death associated protein 3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 45.582414 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #33: protein, mS31, MRPS31

ProteinName: mS31, MRPS31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 44.091996 kDa
SourceSpecies: Pig (pig)

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Component #34: protein, Mitochondrial ribosomal protein S33

ProteinName: Mitochondrial ribosomal protein S33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.54465 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #35: protein, Mitochondrial ribosomal protein S34

ProteinName: Mitochondrial ribosomal protein S34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.843818 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #36: protein, Mitochondrial ribosomal protein S35

ProteinName: Mitochondrial ribosomal protein S35 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 37.104809 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #37: protein, Mitochondrial ribosomal protein S37

ProteinName: Mitochondrial ribosomal protein S37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.561903 kDa
SourceSpecies: Pig (pig)

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Component #38: protein, Aurora kinase A interacting protein 1

ProteinName: Aurora kinase A interacting protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.843941 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #39: protein, Mitochondrial ribosomal protein S39

ProteinName: Mitochondrial ribosomal protein S39
Details: Unassigned residues have been built as poly-alanine.
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 76.473766 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #40: protein, 28S ribosomal protein S18b, mitochondrial

ProteinName: 28S ribosomal protein S18b, mitochondrialRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.220465 kDa
SourceSpecies: Pig (pig)
Source (natural)Organ or tissue: liver

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Component #41: ligand, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

LigandName: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.539246 kDa

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Component #42: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 111 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

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Component #43: ligand, SODIUM ION

LigandName: SODIUM IONSodium / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.29905 MDa

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Component #44: ligand, SPERMINE

LigandName: SPERMINE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.20234 kDa

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Component #45: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

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Component #46: ligand, N-FORMYLMETHIONINE

LigandName: N-FORMYLMETHIONINE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.177221 kDa

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Component #47: ligand, GUANOSINE-5'-TRIPHOSPHATE

LigandName: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.52318 kDa

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Component #48: ligand, water

LigandName: water / Number of Copies: 5 / Recombinant expression: No
MassTheoretical: 1.801505 MDa

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Experimental details

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Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionSpecimen conc.: 0.171 mg/ml / pH: 7.6
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277.15 K / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 4 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON III (4k x 4k)

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Image acquisition

Image acquisitionNumber of digital images: 13936

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 139206
3D reconstructionAlgorithm: FOURIER SPACE / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Modeling #1Refinement space: RECIPROCAL / Overall bvalue: 73.799999999999997
Output model

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