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Yorodumi- EMDB-3843: Structure of a native assembly intermediate of the human mitochon... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-3843 | |||||||||
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Title | Structure of a native assembly intermediate of the human mitochondrial ribosome with unfolded interfacial rRNA | |||||||||
Map data | ||||||||||
Sample |
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Function / homology | Function and homology information negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / Complex I biogenesis / negative regulation of ribosome biogenesis / protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / rRNA import into mitochondrion / Respiratory electron transport / iron-sulfur cluster assembly complex / mitochondrial translational termination ...negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / Complex I biogenesis / negative regulation of ribosome biogenesis / protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / rRNA import into mitochondrion / Respiratory electron transport / iron-sulfur cluster assembly complex / mitochondrial translational termination / mitochondrial large ribosomal subunit binding / mitochondrial translational elongation / translation release factor activity, codon nonspecific / microprocessor complex / Mitochondrial translation elongation / positive regulation of mitochondrial translation / Mitochondrial translation termination / Mitochondrial translation initiation / [2Fe-2S] cluster assembly / Glyoxylate metabolism and glycine degradation / mitochondrial fission / mitochondrial large ribosomal subunit / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / peptidyl-tRNA hydrolase / mitochondrial small ribosomal subunit / acyl binding / aminoacyl-tRNA hydrolase activity / mitochondrial ribosome / iron-sulfur cluster assembly / acyl carrier activity / mitochondrial translation / proton motive force-driven mitochondrial ATP synthesis / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / ribosomal large subunit binding / anatomical structure morphogenesis / RNA processing / rescue of stalled ribosome / aerobic respiration / ribosomal large subunit biogenesis / cellular response to leukemia inhibitory factor / fatty acid binding / mitochondrial membrane / small ribosomal subunit rRNA binding / fibrillar center / fatty acid biosynthetic process / large ribosomal subunit rRNA binding / double-stranded RNA binding / cell junction / 5S rRNA binding / endonuclease activity / mitochondrial inner membrane / negative regulation of translation / rRNA binding / nuclear body / ribosome / mitochondrial matrix / structural constituent of ribosome / cell cycle / translation / ribonucleoprotein complex / protein domain specific binding / nucleotide binding / mRNA binding / synapse / apoptotic process / calcium ion binding / nucleolus / mitochondrion / extracellular space / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) / Human (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.03 Å | |||||||||
Authors | Brown A / Rathore S / Kimanius D / Aibara S / Bai XC / Rorbach J / Amunts A / Ramakrishnan V | |||||||||
Citation | Journal: Nat Struct Mol Biol / Year: 2017 Title: Structures of the human mitochondrial ribosome in native states of assembly. Authors: Alan Brown / Sorbhi Rathore / Dari Kimanius / Shintaro Aibara / Xiao-Chen Bai / Joanna Rorbach / Alexey Amunts / V Ramakrishnan / Abstract: Mammalian mitochondrial ribosomes (mitoribosomes) have less rRNA content and 36 additional proteins compared with the evolutionarily related bacterial ribosome. These differences make the assembly of ...Mammalian mitochondrial ribosomes (mitoribosomes) have less rRNA content and 36 additional proteins compared with the evolutionarily related bacterial ribosome. These differences make the assembly of mitoribosomes more complex than the assembly of bacterial ribosomes, but the molecular details of mitoribosomal biogenesis remain elusive. Here, we report the structures of two late-stage assembly intermediates of the human mitoribosomal large subunit (mt-LSU) isolated from a native pool within a human cell line and solved by cryo-EM to ∼3-Å resolution. Comparison of the structures reveals insights into the timing of rRNA folding and protein incorporation during the final steps of ribosomal maturation and the evolutionary adaptations that are required to preserve biogenesis after the structural diversification of mitoribosomes. Furthermore, the structures redefine the ribosome silencing factor (RsfS) family as multifunctional biogenesis factors and identify two new assembly factors (L0R8F8 and mt-ACP) not previously implicated in mitoribosomal biogenesis. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_3843.map.gz | 13.7 MB | EMDB map data format | |
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Header (meta data) | emd-3843-v30.xml emd-3843.xml | 71.3 KB 71.3 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_3843_fsc.xml | 12.1 KB | Display | FSC data file |
Images | emd_3843.png | 266.3 KB | ||
Masks | emd_3843_msk_1.map | 166.4 MB | Mask map | |
Others | emd_3843_half_map_1.map.gz emd_3843_half_map_2.map.gz | 130.6 MB 130.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-3843 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-3843 | HTTPS FTP |
-Related structure data
Related structure data | 5oomMC 3842C 5oolC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_3843.map.gz / Format: CCP4 / Size: 166.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | emd_3843_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_3843_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_3843_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Mitochondrial ribosome large subunit with assembly factor
+Supramolecule #1: Mitochondrial ribosome large subunit with assembly factor
+Macromolecule #1: 16S ribosomal RNA
+Macromolecule #2: mitochondrial tRNAVal
+Macromolecule #3: 39S ribosomal protein L2, mitochondrial
+Macromolecule #4: 39S ribosomal protein L3, mitochondrial
+Macromolecule #5: 39S ribosomal protein L4, mitochondrial
+Macromolecule #6: 39S ribosomal protein L9, mitochondrial
+Macromolecule #7: 39S ribosomal protein L10, mitochondrial
+Macromolecule #8: 39S ribosomal protein L11, mitochondrial
+Macromolecule #9: 39S ribosomal protein L13, mitochondrial
+Macromolecule #10: 39S ribosomal protein L14, mitochondrial
+Macromolecule #11: 39S ribosomal protein L15, mitochondrial
+Macromolecule #12: 39S ribosomal protein L16, mitochondrial
+Macromolecule #13: 39S ribosomal protein L17, mitochondrial
+Macromolecule #14: 39S ribosomal protein L18, mitochondrial
+Macromolecule #15: 39S ribosomal protein L19, mitochondrial
+Macromolecule #16: 39S ribosomal protein L20, mitochondrial
+Macromolecule #17: 39S ribosomal protein L21, mitochondrial
+Macromolecule #18: 39S ribosomal protein L22, mitochondrial
+Macromolecule #19: 39S ribosomal protein L23, mitochondrial
+Macromolecule #20: 39S ribosomal protein L24, mitochondrial
+Macromolecule #21: 39S ribosomal protein L27, mitochondrial
+Macromolecule #22: 39S ribosomal protein L28, mitochondrial
+Macromolecule #23: 39S ribosomal protein L47, mitochondrial
+Macromolecule #24: 39S ribosomal protein L30, mitochondrial
+Macromolecule #25: 39S ribosomal protein L32, mitochondrial
+Macromolecule #26: 39S ribosomal protein L33, mitochondrial
+Macromolecule #27: 39S ribosomal protein L34, mitochondrial
+Macromolecule #28: 39S ribosomal protein L35, mitochondrial
+Macromolecule #29: 39S ribosomal protein L37, mitochondrial
+Macromolecule #30: 39S ribosomal protein L38, mitochondrial
+Macromolecule #31: 39S ribosomal protein L39, mitochondrial
+Macromolecule #32: 39S ribosomal protein L40, mitochondrial
+Macromolecule #33: 39S ribosomal protein L41, mitochondrial
+Macromolecule #34: 39S ribosomal protein L42, mitochondrial
+Macromolecule #35: 39S ribosomal protein L43, mitochondrial
+Macromolecule #36: 39S ribosomal protein L44, mitochondrial
+Macromolecule #37: 39S ribosomal protein L45, mitochondrial
+Macromolecule #38: 39S ribosomal protein L46, mitochondrial
+Macromolecule #39: 39S ribosomal protein L48, mitochondrial
+Macromolecule #40: 39S ribosomal protein L49, mitochondrial
+Macromolecule #41: 39S ribosomal protein L50, mitochondrial
+Macromolecule #42: 39S ribosomal protein L51, mitochondrial
+Macromolecule #43: 39S ribosomal protein L52, mitochondrial
+Macromolecule #44: 39S ribosomal protein L53, mitochondrial
+Macromolecule #45: 39S ribosomal protein L54, mitochondrial
+Macromolecule #46: 39S ribosomal protein L55, mitochondrial
+Macromolecule #47: Ribosomal protein 63, mitochondrial
+Macromolecule #48: Peptidyl-tRNA hydrolase ICT1, mitochondrial
+Macromolecule #49: Growth arrest and DNA damage-inducible proteins-interacting protein 1
+Macromolecule #50: 39S ribosomal protein S18a, mitochondrial
+Macromolecule #51: 39S ribosomal protein S30, mitochondrial
+Macromolecule #52: Unknown protein or protein extension
+Macromolecule #53: Mitochondrial assembly of ribosomal large subunit protein 1
+Macromolecule #54: MIEF1 upstream open reading frame protein
+Macromolecule #55: Acyl carrier protein, mitochondrial
+Macromolecule #56: MAGNESIUM ION
+Macromolecule #57: ZINC ION
+Macromolecule #58: 4'-PHOSPHOPANTETHEINE
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.45 Component:
Details: 2 mM Synercid (Santa Cruz Biotechnology, Inc) was added to reduce preferential orientation. | |||||||||||||||
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Grid | Model: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3.0 nm / Pretreatment - Type: GLOW DISCHARGE | |||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: Sample incubated for 30 s prior to freezing.. | |||||||||||||||
Details | Sample concentration = 100 nM |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Calibrated magnification: 130841 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Frames/image: 1-25 / Average exposure time: 1.5 sec. / Average electron dose: 1.56 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |