EMDB-10380: Structure of rabbit 80S ribosome translating beta-tubulin in comp...

Basic information

• Entry: Database: EMDB / ID: EMD-10380
• Title: Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 and nascent chain-associated complex
• Map data: Structure of human TTC5 bound to the rabbit 80S ribosome translating beta-tubulin Sharpened, MTF-corrected map with the calibrated pixel size

Sample

• Complex: Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5
  • Complex: Ribosome
    • Protein or peptide: x 48 types
    • RNA: x 4 types
  • Complex: Tetratricopeptide repeat protein 5
    • Protein or peptide: x 1 types
• Ligand: x 2 types

Function / homology

Function:

positive regulation of mRNA catabolic process / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cellular response to starvation / cytosolic ribosome / Regulation of TP53 Activity through Methylation / ribosome binding / 5S rRNA binding / cytosolic large ribosomal subunit / cytoplasmic vesicle / ribosome / mitochondrial matrix / structural constituent of ribosome / ribonucleoprotein complex / translation / DNA repair / DNA damage response / chromatin binding / nucleolus / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / RNA binding / nucleoplasm / cytosol / cytoplasm

Domain/homology:

Tetratricopeptide repeat protein 5, OB fold domain / TTC5, OB fold domain superfamily / Tetratricopeptide repeat protein 5 OB fold domain / Ribosomal protein L44e signature. / Ribosomal protein L27e signature. / Ribosomal protein L10e signature. / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L39e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e signature. / Ribosomal protein L6e signature. / Ribosomal protein L30e signature 1. / Ribosomal protein L30e signature 2. / Ribosomal protein L36e signature. / Ribosomal protein L39e signature. / Ribosomal protein L35Ae signature. / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / TPR repeat region circular profile. / Ribosomal protein L31e signature. / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L7/L30 / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L32e signature. / Ribosomal protein L6 signature 2. / TPR repeat profile. / Ribosomal protein L15e signature. / Ribosomal protein L21e signature. / Ribosomal protein L37e signature. / Tetratricopeptide repeats / Tetratricopeptide repeat / Ribosomal protein L11 signature. / Ribosomal protein L23 signature. / Ribosomal protein L5 signature. / Ribosomal protein L29 signature. / Ribosomal protein L15 signature. / Ribosomal protein L14 signature. / Ribosomal protein L22 signature. / Ribosomal protein L30, ferredoxin-like fold domain / Tetratricopeptide-like helical domain superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L30p/L7e / Ribosomal protein L24 signature. / Ribosomal protein L3 signature.

Component:

Small ribosomal subunit protein eS32 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL34 / Tetratricopeptide repeat protein 5 / Large ribosomal subunit protein eL37

• Biological species: Oryctolagus cuniculus (rabbit) / Homo sapiens (human) / Rabbit (rabbit)
• Method: single particle reconstruction / cryo EM / Resolution: 3.11 Å
• Authors: Lin Z / Gasic I / Chandrasekaran V / Peters N / Shao S / Ramakrishnan V / Mitchison TJ / Hegde RS

Funding support

United Kingdom, 3 items

Organization	Grant number	Country
Medical Research Council (United Kingdom)	MC_UP_A022_1007	United Kingdom
Wellcome Trust	WT096570	United Kingdom
Medical Research Council (United Kingdom)	MC_U105184332	United Kingdom

• Citation: Journal: Science / Year: 2020; Title: TTC5 mediates autoregulation of tubulin via mRNA degradation.; Authors: Zhewang Lin / Ivana Gasic / Viswanathan Chandrasekaran / Niklas Peters / Sichen Shao / Timothy J Mitchison / Ramanujan S Hegde / ; Abstract: Tubulins play crucial roles in cell division, intracellular traffic, and cell shape. Tubulin concentration is autoregulated by feedback control of messenger RNA (mRNA) degradation via an unknown mechanism. We identified tetratricopeptide protein 5 (TTC5) as a tubulin-specific ribosome-associating factor that triggers cotranslational degradation of tubulin mRNAs in response to excess soluble tubulin. Structural analysis revealed that TTC5 binds near the ribosome exit tunnel and engages the amino terminus of nascent tubulins. TTC5 mutants incapable of ribosome or nascent tubulin interaction abolished tubulin autoregulation and showed chromosome segregation defects during mitosis. Our findings show how a subset of mRNAs can be targeted for coordinated degradation by a specificity factor that recognizes the nascent polypeptides they encode.

History

Deposition	Oct 15, 2019	-
Header (metadata) release	Nov 27, 2019	-
Map release	Nov 27, 2019	-
Update	Dec 2, 2020	-
Current status	Dec 2, 2020	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_10380.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Structure of human TTC5 bound to the rabbit 80S ribosome translating beta-tubulin Sharpened, MTF-corrected map with the calibrated pixel size
• Voxel size: X=Y=Z: 1.33 Å

Density

Contour Level	By AUTHOR: 0.08 / Movie #1: 0.08
Minimum - Maximum	-0.3713348 - 0.6941945
Average (Standard dev.)	0.0007830047 (±0.017911052)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 532.0 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.33	1.33	1.33
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	532.000	532.000	532.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	-42	0	-29
NX/NY/NZ	88	78	86
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.371	0.694	0.001

Supplemental data

Mask #1

• File: emd_10380_msk_1.map

Additional map: Unsharpened map Note that the pixel size has not been calibrated.

• File: emd_10380_additional.map
• Annotation: Unsharpened map Note that the pixel size has not been calibrated.

Half map: Half1 map Note that the pixel size has not been calibrated.

• File: emd_10380_half_map_1.map
• Annotation: Half1 map Note that the pixel size has not been calibrated.

Half map: Half2 map Note that the pixel size has not been calibrated.

• File: emd_10380_half_map_2.map
• Annotation: Half2 map Note that the pixel size has not been calibrated.

Sample components

Entire : Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5

• Entire: Name: Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5

Components

• Complex: Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5
  • Complex: Ribosome
    • Protein or peptide: Ribosomal protein L8
    • Protein or peptide: uL3
    • Protein or peptide: uL4
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: Ul30
    • Protein or peptide: eL8
    • Protein or peptide: uL6
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: Ribosomal protein L11
    • Protein or peptide: eL13
    • Protein or peptide: Ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: uL13
    • Protein or peptide: uL22
    • Protein or peptide: eL18
    • Protein or peptide: eL19
    • Protein or peptide: eL20
    • Protein or peptide: eL21
    • Protein or peptide: eL22
    • Protein or peptide: Ribosomal protein L23
    • Protein or peptide: eL24
    • Protein or peptide: uL23
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: uL15
    • Protein or peptide: eL29
    • Protein or peptide: eL30
    • Protein or peptide: eL31
    • Protein or peptide: eL32CD59
    • Protein or peptide: eL33
    • Protein or peptide: eL34
    • Protein or peptide: uL29
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: eL38
    • Protein or peptide: eL39
    • Protein or peptide: eL40
    • Protein or peptide: 60s ribosomal protein l41
    • Protein or peptide: eL42
    • Protein or peptide: eL43
    • Protein or peptide: eL28
    • Protein or peptide: uL10
    • Protein or peptide: Ribosomal protein L12
    • RNA: P-site tRNA
    • RNA: 28S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • Protein or peptide: Nascent polypeptide-associated complex subunit alpha N-terminal region
    • Protein or peptide: Nascent polypeptide-associated complex subunit alpha
    • Protein or peptide: Transcription factor BTF3
    • Protein or peptide: Tubulin Beta
  • Complex: Tetratricopeptide repeat protein 5
    • Protein or peptide: Tetratricopeptide repeat protein 5
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION

Supramolecule #1: Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5

• Supramolecule: Name: Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53; Details: The 40S is not modelled because it has been signal-subtracted.
• Molecular weight: Theoretical: 4.5 MDa

Supramolecule #2: Ribosome

• Supramolecule: Name: Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#51, #53
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)

Supramolecule #3: Tetratricopeptide repeat protein 5

• Supramolecule: Name: Tetratricopeptide repeat protein 5 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #52
• Source (natural): Organism: Homo sapiens (human)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Macromolecule #1: Ribosomal protein L8

• Macromolecule: Name: Ribosomal protein L8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 28.088863 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GTKTVQEKEN

Macromolecule #2: uL3

• Macromolecule: Name: uL3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 46.107977 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKTHRG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINPL GGF VHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKE EGA

Macromolecule #3: uL4

• Macromolecule: Name: uL4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 47.727559 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLNIL KL APGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KKVKKPRAVG IKQKKKPVVG RKAA AAKKP AADKKAADKR AGPEDKKPAA

Macromolecule #4: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 34.481828 KDa

Sequence

String:

MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

Macromolecule #5: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 33.028336 KDa

Sequence

String:

MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

Macromolecule #6: Ul30

• Macromolecule: Name: Ul30 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 29.201836 KDa

Sequence

String:

MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL TDNTLIARSL GKYNIICMED LIHEIYTVGK HFKEANNFLW PFKLSSPRGG MKKKTTHFVE GGDAGNREDQ IN RLIRRMN

Macromolecule #7: eL8

• Macromolecule: Name: eL8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 36.221516 KDa

Sequence

String:

MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ARAEKKAAGK GDVPTKRPPV LRAGVNTVTT LVENKKAQLV VIAHDVDPIE LVVFLPALCR KMGVPYCILK GK ARLGRLV HRKTCTTVAF TQVNSEDKGA LAKLVEAIRT NYNDRYDEIR RHWGGNVLGP KSVARIAKLE KAKAKELATK LG

Macromolecule #8: uL6

• Macromolecule: Name: uL6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 21.871418 KDa

Sequence

String:

MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

Macromolecule #9: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.643057 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

Macromolecule #10: Ribosomal protein L11

• Macromolecule: Name: Ribosomal protein L11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

Macromolecule #11: eL13

• Macromolecule: Name: eL13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.331723 KDa

Sequence

String:

MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

Macromolecule #12: Ribosomal protein L14

• Macromolecule: Name: Ribosomal protein L14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 23.870549 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

Macromolecule #13: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

Macromolecule #14: uL13

• Macromolecule: Name: uL13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.533299 KDa

Sequence

String:

MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK ISKFTDVLKT HGLLV

Macromolecule #15: uL22

• Macromolecule: Name: uL22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 21.444221 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

Macromolecule #16: eL18

• Macromolecule: Name: eL18 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.653596 KDa

Sequence

String:

GVDIRHNKDR KVRRKEPKSQ DIYLRLLVKL YRFLARRTNS TFNQVVLKRL FMSRTNRPPL SLSRMIRKMK LPGRENKTAV VVGTVTDDV RVQEVPKLKV CALRVTSRAR SRILKAGGKI LTFDQLALDS PKGRGTVLLS GPRKGREVYR HFGKAPGTPH S HTKPYVRS KGRKFERARG RRASRGYKN(UNK)

Macromolecule #17: eL19

• Macromolecule: Name: eL19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

Macromolecule #18: eL20

• Macromolecule: Name: eL20 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.827561 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCRTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

Macromolecule #19: eL21

• Macromolecule: Name: eL21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

Macromolecule #20: eL22

• Macromolecule: Name: eL22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.813015 KDa

Sequence

String:

MAPVKKLVAK GGKKKKQLLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VSLERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANTKES YELRYFQINQ DEEEEEEED

Macromolecule #21: Ribosomal protein L23

• Macromolecule: Name: Ribosomal protein L23 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

Macromolecule #22: eL24

• Macromolecule: Name: eL24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

Macromolecule #23: uL23

• Macromolecule: Name: uL23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 17.768246 KDa

Sequence

String:

MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

Macromolecule #24: Ribosomal protein L26

• Macromolecule: Name: Ribosomal protein L26 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

Macromolecule #25: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

Macromolecule #26: uL15

• Macromolecule: Name: uL15 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 16.620561 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGMHH HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

Macromolecule #27: eL29

• Macromolecule: Name: eL29 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.931625 KDa

Sequence

String:

MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPA

Macromolecule #28: eL30

• Macromolecule: Name: eL30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.807065 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQSLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

Macromolecule #29: eL31

• Macromolecule: Name: eL31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

Macromolecule #30: eL32

• Macromolecule: Name: eL32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

Macromolecule #31: eL33

• Macromolecule: Name: eL33 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.580809 KDa

Sequence

String:

MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

Macromolecule #32: eL34

• Macromolecule: Name: eL34 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 13.196894 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKA

Macromolecule #33: uL29

• Macromolecule: Name: uL29 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.566599 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ESLKTKKQQR KERLYPLRKY AVKA

Macromolecule #34: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.263834 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSSVLAAMRK AAAKKD

Macromolecule #35: Ribosomal protein L37

• Macromolecule: Name: Ribosomal protein L37 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

Macromolecule #36: eL38

• Macromolecule: Name: eL38 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 8.238948 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

Macromolecule #37: eL39

• Macromolecule: Name: eL39 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 6.455775 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIWMK TGNKIRYNSK RRHWRRTKLG L

Macromolecule #38: eL40

• Macromolecule: Name: eL40 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.69979 KDa

Sequence

String:

MGDPESGGCI PPDQQRLIFA GKQLEDGRTL SDYNIQKEST LHLVLRLRGG IIEPSLRQLA QKYNCDKMIC RKCYARLHPR AVNCRKKKC GHTNNLRPKK KVK

Macromolecule #39: 60s ribosomal protein l41

• Macromolecule: Name: 60s ribosomal protein l41 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

Macromolecule #40: eL42

• Macromolecule: Name: eL42 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.476973 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

Macromolecule #41: eL43

• Macromolecule: Name: eL43 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 10.29935 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

Macromolecule #42: eL28

• Macromolecule: Name: eL28 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.783614 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYHPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

Macromolecule #43: uL10

• Macromolecule: Name: uL10 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.380504 KDa

Sequence

String:

MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

Macromolecule #44: Ribosomal protein L12

• Macromolecule: Name: Ribosomal protein L12 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

Macromolecule #49: Nascent polypeptide-associated complex subunit alpha N-terminal region

• Macromolecule: Name: Nascent polypeptide-associated complex subunit alpha N-terminal region; type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 2.147389 KDa

Sequence

String:

AAAAAAAAAA LAAAAAPAAA AAAAAAAAA

Macromolecule #50: Nascent polypeptide-associated complex subunit alpha

• Macromolecule: Name: Nascent polypeptide-associated complex subunit alpha / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.406824 KDa

Sequence

String:

MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVT GVTRVTIRKS KNILFVITKP DVYKSPASDT YIVFGEAKIE DLSQQAQLAA AEKFKVQGEA VSNIQENTQT P TVQEESEE EEVDETGVEV KDIELVMSQA NVSRAKAVRA LKNNSNDIVN AIMELTM

Macromolecule #51: Transcription factor BTF3

• Macromolecule: Name: Transcription factor BTF3 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.169984 KDa

Sequence

String:

MRRTGAPAQA DSRGRGRARG GCPGGEATPS LPPPRGGTRG QEPQMKETIM NQEKLAKLQA QVRIGGKGTA RRKKKVVHRT ATADDKKLQ FSLKKLGVNN ISGIEEVNMF TNQGTVIHFN NPKVQASLAA NTFTITGHAE TKQLTEMLPS ILNQLGADSL T SLRRLAEA LPKQSVDGKA PLATGEDDDD EVPDLVENFD EASKNEAN

Macromolecule #52: Tetratricopeptide repeat protein 5

• Macromolecule: Name: Tetratricopeptide repeat protein 5 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 48.988418 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MMADEEEEVK PILQKLQELV DQLYSFRDCY FETHSVEDAG RKQQDVQKEM EKTLQQMEEV VGSVQGKAQV LMLTGKALNV TPDYSPKAE ELLSKAVKLE PELVEAWNQL GEVYWKKGDV AAAHTCFSGA LTHCRNKVSL QNLSMVLRQL RTDTEDEHSH H VMDSVRQA KLAVQMDVHD GRSWYILGNS YLSLYFSTGQ NPKISQQALS AYAQAEKVDR KASSNPDLHL NRATLHKYEE SY GEALEGF SRAAALDPAW PEPRQREQQL LEFLDRLTSL LESKGKVKTK KLQSMLGSLR PAHLGPCSDG HYQSASGQKV TLE LKPLST LQPGVNSGAV ILGKVVFSLT TEEKVPFTFG LVDSDGPCYA VMVYNIVQSW GVLIGDSVAI PEPNLRLHRI QHKG KDYSF SSVRVETPLL LVVNGKPQGS SSQAVATVAS RPQCE

Macromolecule #53: Tubulin Beta

• Macromolecule: Name: Tubulin Beta / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 7.163992 KDa

Sequence

String:

MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLDRISVY YNEATGGKYV MKLV

Macromolecule #45: P-site tRNA

• Macromolecule: Name: P-site tRNA / type: rna / ID: 45 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.436551 KDa

Sequence

String:

GUCUCCGUAG UGUAGCGGUU AUCACGUUCG CCUAACACGC GAAAGGUCCU CGGUUCGAAA CCGGGCGGAA ACACCA

Macromolecule #46: 28S ribosomal RNA

• Macromolecule: Name: 28S ribosomal RNA / type: rna / ID: 46 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 1.148115375 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGGGGGGC CCAAGUCCUU CUGAUCGAGG CCCAGCCCGU GGACGGUGUG AGGCCGGUAG CG GCCCCCG GCGCGCCGGG CCCGGGUCUU CCCGGAGUCG GGUUGCUUGG GAAUGCAGCC CAAAGCGGGU GGUAAACUCC AUC UAAGGC UAAAUACCGG CACGAGACCG AUAGUCAACA AGUACCGUAA GGGAAAGUUG AAAAGAACUU UGAAGAGAGA GUUC AAGAG GGCGUGAAAC CGUUAAGAGG UAAACGGGUG GGGUCCGCGC AGUCCGCCCG GAGGAUUCAA CCCGGCGGCG CGGUC CGGC CGUGCCGGCG GGGCGGAUCU CCCGCUCCCG UCGGCGGGGG ACCGCCCCCC GGCCGGCGAC CGGCCGCCGC CGGGCG CAU UUCCACCGCG GCGGUGCGCC GCGACCGGCU CCGGGACGGC UGGGAAGGCC CCGGCGGGGA AGGUGGCCCG GGGGCCC CG GGUGUUACAG CCCCGCCCCG GCAGCAGCAG CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG ACCCGUGCGC CGCGCUCU C CCCCCUCGGG GGGGCCGGGC CACCCCUCCC ACGGCGCGAC CGCUGGGGCG GACUGUCCAG UGCGCCCCGG GCGGGUCGC GCCGUCGGGC CCGGGGGGCC ACGCGUCCCC CGCGAGCGCA CGGGGUCGGC GGCGAUGUCG GCUACCCACC CGACCCGUCU UGAAACACG GACCAAGGAG UCUAACACGU GCGCGAGUCG GGGGCUACGA AAGCCGCCGU GGCGCAAUGA AGGUGAAGGC C GGCGGCGC GCCGCCGGCC GAGGUGGGAU CCCGAGGCCU UCCAGUCCGC CGAGGGCGCA CCACCGGCCC GUCUCGCCCG CC GCGCCGG GGAGGUGGAG CACGAGCGCA CGUGUUAGGA CCCGAAAGAU GGUGAACUAU GCCUGGGCAG GGCGAAGCCA GAG GAAACU CUGGUGGAGG UCCGUAGCGG UCCUGACGUG CGAAUCGGUC GUCCGACCUG GGUAUAGGGG CGAAAGACUA AUCG AACCA UCUAGUAGCU GGUUCCCUCC AAAGUUUCCC UCAGGAUAGC UGGCGCUCUC CGCAGUUUUA UCCGGUAAAG CGAAU GAUU AGAGGUCUUG GGGCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GUGUGG AGC CGGGCGUGGA AUGCGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGG UU AAGGCGCCCG AUGCCGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGAUGGUGG CCAUGGAA G UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGG GCCCAUACCC GGCCGUCGCU GGCAGUCGGC AGAGCGAGAA AGAGAGUGGC GCGGACGCUA CGCCGCGACG AGUAGGAGGG CCGCUGCGG UGAGCCUUGA AGCCUAGGGC GCGGGCCCGG GUGGAGCCGC CGCAGGUGCA GAUCUUGGUG GUAGUAGCAA A UAUUCAAA CGAGAACUUU GAAGGCCGAA GUGGAGAAGG GUUCCAUGUG AACAGCAGUU GAACAUGGGU CAGUCGGUCC UG AGAGAUG GGCGAGCGCC GUUCCGAAGG GACGGGCGAU GGCCUCCGUU GCCCUCAGCC GAUCGAAAGG GAGUCGGGUU CAG AUCCCC GAAUCCGGAG UGGCGGAGAU GGGCGCCGCG AGGCGUCCAG UGCGGUAACG CGACCGAUCC CGGAGAAGCC GGCG GGAGC CCCGGGGAGA GUUCUCUUUU CUUUGUGAAG GGCAGGGCGC CCUGGAAUGG GUUCGCCCCG AGAGAGGGGC CCGUG CCUU GGAAAGCGUC GCGGUUCCGG CGGCGUCCGG UGAGCUCUCG CUGGCCCUUG AAAAUCCGGG GGAGAGGGUG UAAAUC UCG CGCCGGGCCG UACCCAUAUC CGCAGCAGGU CUCCAAGGUG AACAGCCUCU GGCAUGUUGG AACAAUGUAG GUAAGGG AA GUCGGCAAGC CGGAUCCGUA ACUUCGGGAU AAGGAUUGGC UCUAAGGGCU GGCAGCCGAC UUAGAACUGG UGCGGACC A GGGGAAUCCG ACUGUUUAAU UAAAACAAAG CAUCGCGAAG GCCCGCGGCG GGUGUUGACG CGAUGUGAUU UCUGCCCAG UGCUCUGAAU GUCAAAGUGA AGAAAUUCAA UGAAGCGCGG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAGCCA AAUGCCUCG UCAUCUAAUU AGUGACGCGC AUGAAUGGAU GAACGAGAUU CCCACUGUCC CUACCUACUA UCCAGCGAAA C CACAGCCA AGGGAACGGG CUUGGCGGAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUC UGGCACGGUG AA GAGACGU UUUUUCACUG ACCCGGUGAG GCGGGGGGGC GAGCCGGGCU CUCGCUUCUG GCGCCAAGCG CCCGGCCGGC CGG GCGCGA CCCGCUCCGG GGACAGUGCC AGGUGGGGAG UUUGACUGGG GCGGUACACC UGUCAAACGG UAACGCAGGU GUCC UAAGG CGAGCUCAGG GAGGACAGAA ACCUCCCGUG GAGCAGAAGG GCAAAAGCUC GCUUGAUCUU GAUUUUCAGU ACGAA UACA GACCGUGAAA GCGGGGCCUC ACGAUCCUUC UGACCUUUUG GGUUUUAAGC AGGAGGUGUC AGAAAAGUUA CCACAG GGA UAACUGGCUU GUGGCGGCCA AGCGUUCAUA GCGACGUCGC UUUUUGAUCC UUCGAUGUCG GCUCUUCCUA UCAUUGU GA AGCAGAAUUC ACCAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGU U AGUUUUACCC UACUGAUGAU GUGUUGUUGC CAUGGUAAUC CUGCUCAGUA CGAGAGGAAC CGCAGGUUCA GACAUUUGG UGUAUGUGCU UGGCUGAGGA GCCAAUGGGG CGAAGCUACC AUCUGUGGGA UUAUGACUGA ACGCCUCUAA GUCAGAAUCC CGCCCAGGC GGAACGAUAG CGCCGGAGCC UCGGUUGGCC UCGGAUAGCC GGUCCCCCGC CGGGGUCCGG UGCGGAGAGC C CUUCGUCC CGGGAAACGG GGCGGCCGGA AAGGGGGCCG CCCUCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU GG CGCUAAA CCAUUCGUAG ACGACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUGCUGCG AUCUAUUGAA AGU CAGCCC UCGACACAAG GGUUUGU

Macromolecule #47: 5S ribosomal RNA

• Macromolecule: Name: 5S ribosomal RNA / type: rna / ID: 47 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 38.691914 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

Macromolecule #48: 5.8S ribosomal RNA

• Macromolecule: Name: 5.8S ribosomal RNA / type: rna / ID: 48 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 50.143648 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCU

Macromolecule #54: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 220 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #55: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 55 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 48.36 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

10181

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.11 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 49626