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- EMDB-10181: Rabbit 80S ribosome stalled on a poly(A) tail -

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Basic information

Entry
Database: EMDB / ID: EMD-10181
TitleRabbit 80S ribosome stalled on a poly(A) tail
Map data
SampleRabbit 80S ribosome stalled on polyA mRNA
  • ribosome
  • polyA mRNA
  • (nucleic-acidNucleic acid) x 6
  • uS2
  • (40S ribosomal protein ...) x 8
  • (uS5) x 2
  • (Ribosomal protein ...) x 15
  • S10_plectin domain-ontaining protein
  • Ribosomal_S13_N domain-containing protein
  • uS11
  • uS19
  • eS17
  • eS13
  • eS19
  • Ribosomal_S10 domain-containing protein
  • eS21
  • uS12
  • eS24
  • eS25
  • eS26
  • uS14
  • WD_REPEATS_REGION domain-containing protein
  • uL3
  • uL4
  • (60S ribosomal protein ...) x 6
  • uL30
  • eL8
  • uL6
  • eL13
  • uL13
  • uL22
  • eL18
  • eL19
  • eL20
  • eL21
  • eL22
  • TRASH domain-containing protein
  • Ribosomal_L23eN domain-containing protein
  • Ribosomal_L18e/L15P domain-containing protein
  • eL29
  • Ribosomal_L7Ae domain-containing protein
  • eL31
  • eL32CD59
  • eL33
  • eL34
  • uL29
  • eL38
  • eL39
  • eL40
  • eL42
  • eL43
  • Ribosomal_L28e domain-containing protein
  • uL10
  • poly-lysine nascent chain
  • Ribosomal protein
  • (ligand) x 3
Function / homology
Function and homology information


oxidized pyrimidine DNA binding / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / response to TNF agonist / positive regulation of DNA N-glycosylase activity / regulation of translation involved in cellular response to UV / positive regulation of base-excision repair / negative regulation of DNA repair / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage ...oxidized pyrimidine DNA binding / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / response to TNF agonist / positive regulation of DNA N-glycosylase activity / regulation of translation involved in cellular response to UV / positive regulation of base-excision repair / negative regulation of DNA repair / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / NF-kappaB complex / mammalian oogenesis stage / cytosolic ribosome / positive regulation of endodeoxyribonuclease activity / protein kinase A binding / activation-induced cell death of T cells / supercoiled DNA binding / ubiquitin-like protein conjugating enzyme binding / positive regulation of T cell receptor signaling pathway / positive regulation of activated T cell proliferation / erythrocyte development / oxidized purine DNA binding / TOR signaling / T cell proliferation involved in immune response / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / spindle assembly / positive regulation of interleukin-2 production / ribosomal small subunit biogenesis / ribosomal large subunit biogenesis / positive regulation of JUN kinase activity / gastrulation / positive regulation of microtubule polymerization / polysome / polysomal ribosome / mitotic cell cycle checkpoint / Hsp70 protein binding / cellular response to leukemia inhibitory factor / endodeoxyribonuclease activity / negative regulation of protein ubiquitination / small ribosomal subunit rRNA binding / Hsp90 protein binding / DNA-(apurinic or apyrimidinic site) endonuclease activity / mRNA 5'-UTR binding / mitotic spindle / small ribosomal subunit / chromosome segregation / positive regulation of translation / DNA damage response, detection of DNA damage / cytoplasmic ribonucleoprotein granule / cytoplasmic translation / rRNA processing / T cell differentiation in thymus / ruffle membrane / placenta development / cellular response to gamma radiation / positive regulation of NIK/NF-kappaB signaling / cell body / large ribosomal subunit / G1/S transition of mitotic cell cycle / cytosolic large ribosomal subunit / 5S rRNA binding / cytosolic small ribosomal subunit / cellular response to hydrogen peroxide / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / viral capsid / cellular response to tumor necrosis factor / glucose homeostasis / RNA polymerase II regulatory region sequence-specific DNA binding / mitochondrial inner membrane / negative regulation of translation / ribosome / rRNA binding / microtubule binding / killing of cells of other organism / structural constituent of ribosome / antimicrobial humoral immune response mediated by antimicrobial peptide / mitochondrial matrix / postsynaptic density / positive regulation of NF-kappaB transcription factor activity / cell differentiation / translation / mRNA binding / protein-containing complex binding / defense response to Gram-negative bacterium / positive regulation of apoptotic process / dendrite / transcription factor binding / nucleolus / structural molecule activity / positive regulation of gene expression / protein kinase binding / endoplasmic reticulum / negative regulation of apoptotic process / Golgi apparatus / perinuclear region of cytoplasm / RNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus
Ribosomal protein L30, N-terminal / Ribosomal protein L23/L15e core domain superfamily / Ribosomal protein S30 / Ribosomal protein L41 / Ribosomal protein L34Ae / Translation protein SH3-like domain superfamily / Translation protein, beta-barrel domain superfamily / K homology domain superfamily, prokaryotic type / S15/NS1, RNA-binding / Ribosomal protein S13-like, H2TH ...Ribosomal protein L30, N-terminal / Ribosomal protein L23/L15e core domain superfamily / Ribosomal protein S30 / Ribosomal protein L41 / Ribosomal protein L34Ae / Translation protein SH3-like domain superfamily / Translation protein, beta-barrel domain superfamily / K homology domain superfamily, prokaryotic type / S15/NS1, RNA-binding / Ribosomal protein S13-like, H2TH / TRASH domain / Ribosomal protein L37ae/L37e / Zinc-binding ribosomal protein / Nucleic acid-binding, OB-fold / Ribosomal protein S13/S15, N-terminal / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein L25/L23 / Ribosomal protein L24/L26, conserved site / Ribosomal protein S4e, N-terminal / Ribosomal protein S4e, central region / Ribosomal protein S6, eukaryotic / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein L2, domain 2 / Ribosomal protein L2, domain 3 / WD40/YVTN repeat-like-containing domain superfamily / K homology domain-like, alpha/beta / Ribosomal protein L30, ferredoxin-like fold domain / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L10e/L16 / WD40-repeat-containing domain / Ribosomal protein L34e, conserved site / Ribosomal protein S4, conserved site / Ribosomal protein L7, eukaryotic / KOW / Ribosomal protein L29, conserved site / Ribosomal protein L14e domain / WD40 repeat / Outer capsid protein VP2, Orbivirus / Ribosomal protein L35A / Ribosomal protein S10 / Ribosomal protein L29/L35 / Ribosomal protein S13 / Ribosomal protein S4/S9, N-terminal / Ribosomal protein L5 / Ribosomal protein L1 / Ribosomal protein L2 / Ribosomal protein S19/S15 / Ribosomal protein L6, conserved site-2 / Ribosomal protein L28e / Ribosomal protein L38e / Ribosomal protein S27a / Ribosomal protein L26/L24, eukaryotic/archaeal / RNA-binding S4 domain / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / K Homology domain, type 2 / Ribosomal protein S25 / Plectin/S10, N-terminal / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal protein L6, N-terminal / Ribosomal protein L23/L25, N-terminal / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein L10e, conserved site / 40S ribosomal protein S11, N-terminal / Ribosomal protein L1-like / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L2, C-terminal / Ribosomal protein L2, conserved site / Ribosomal protein S4/S9 / Ribosomal protein L5 domain superfamily / Ribosomal protein L30e, conserved site / Ribosomal protein S15P / Ribosomal protein L30, central domain, archaeal type / Ribosomal protein L24e, conserved site / Ribosomal protein S19, superfamily / Ribosomal protein L39e domain superfamily / Ribosomal protein L2, archaeal-type / Ribosomal protein L1, conserved site / Ribosomal protein S7 domain / Ribosomal protein S19 conserved site / Ribosomal protein L15e core domain superfamily / Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal / 30s ribosomal protein S13, C-terminal / Ribosomal protein S10 domain / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein S28e conserved site / Ribosomal L28e/Mak16 / 50S ribosomal protein L30e-like / Ubiquitin-like domain superfamily / Ribosomal protein L15 / Ribosomal protein L5, C-terminal / Ribosomal protein L5, N-terminal
Uncharacterized protein / 60S ribosomal protein L36 / Ribosomal protein S28 / 40S ribosomal protein S8 / 40S ribosomal protein S4 / Uncharacterized protein / 40S ribosomal protein S6 / Uncharacterized protein / KH type-2 domain-containing protein / Uncharacterized protein ...Uncharacterized protein / 60S ribosomal protein L36 / Ribosomal protein S28 / 40S ribosomal protein S8 / 40S ribosomal protein S4 / Uncharacterized protein / 40S ribosomal protein S6 / Uncharacterized protein / KH type-2 domain-containing protein / Uncharacterized protein / S10_plectin domain-containing protein / Ribosomal_S17_N domain-containing protein / Ribosomal_L2_C domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal_S7 domain-containing protein / 60S ribosomal protein L27 / 40S ribosomal protein S27 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal_L28e domain-containing protein / Uncharacterized protein / Uncharacterized protein / Ribosomal_L7Ae domain-containing protein / Ubiquitin-like domain-containing protein / 60s ribosomal protein l41 / Ribosomal protein L10 (Predicted) / Ribosomal protein S9 (Predicted) / TRASH domain-containing protein / Ribosomal_L23eN domain-containing protein / Uncharacterized protein / 40S ribosomal protein S12 / Uncharacterized protein / Outer capsid protein VP2 / Uncharacterized protein / Ribosomal_S10 domain-containing protein / WD_REPEATS_REGION domain-containing protein / 60S ribosomal protein L6 / 40S ribosomal protein S25 / Ribosomal protein / Uncharacterized protein / Ribosomal_L18e/L15P domain-containing protein / Ribosomal_S13_N domain-containing protein / KOW domain-containing protein / 40S ribosomal protein S3a / 40S ribosomal protein S7 / VP5 / 60S ribosomal protein L5 / Ribosomal_L14e domain-containing protein / Ribosomal protein L15 / Uncharacterized protein / 40S ribosomal protein S30 / Ribosomal protein L37
Biological speciesOryctolagus cuniculus (rabbit) / Homo sapiens (human) / Rabbit (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsChandrasekaran V / Juszkiewicz S / Choi J / Puglisi JD / Brown A / Shao S / Ramakrishnan V / Hegde RS
Funding support United Kingdom, United States, 5 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MC_UP_A022_1007 United Kingdom
Wellcome TrustWT096570 United Kingdom
Medical Research Council (United Kingdom)MC_U105184332 United Kingdom
National Institutes of Health/National Institute of General Medical SciencesGM113078 United States
National Institutes of Health/National Institute of General Medical SciencesGM51266 United States
CitationJournal: Nat. Struct. Mol. Biol. / Year: 2019
Title: Mechanism of ribosome stalling during translation of a poly(A) tail.
Authors: Viswanathan Chandrasekaran / Szymon Juszkiewicz / Junhong Choi / Joseph D Puglisi / Alan Brown / Sichen Shao / V Ramakrishnan / Ramanujan S Hegde /
Abstract: Faulty or damaged messenger RNAs are detected by the cell when translating ribosomes stall during elongation and trigger pathways of mRNA decay, nascent protein degradation and ribosome recycling. ...Faulty or damaged messenger RNAs are detected by the cell when translating ribosomes stall during elongation and trigger pathways of mRNA decay, nascent protein degradation and ribosome recycling. The most common mRNA defect in eukaryotes is probably inappropriate polyadenylation at near-cognate sites within the coding region. How ribosomes stall selectively when they encounter poly(A) is unclear. Here, we use biochemical and structural approaches in mammalian systems to show that poly-lysine, encoded by poly(A), favors a peptidyl-transfer RNA conformation suboptimal for peptide bond formation. This conformation partially slows elongation, permitting poly(A) mRNA in the ribosome's decoding center to adopt a ribosomal RNA-stabilized single-stranded helix. The reconfigured decoding center clashes with incoming aminoacyl-tRNA, thereby precluding elongation. Thus, coincidence detection of poly-lysine in the exit tunnel and poly(A) in the decoding center allows ribosomes to detect aberrant mRNAs selectively, stall elongation and trigger downstream quality control pathways essential for cellular homeostasis.
Validation ReportPDB-ID: 6sgc

SummaryFull reportAbout validation report
History
DepositionAug 3, 2019-
Header (metadata) releaseDec 4, 2019-
Map releaseDec 4, 2019-
UpdateDec 4, 2019-
Current statusDec 4, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6sgc
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_10181.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.09 Å/pix.
x 512 pix.
= 555.52 Å
1.09 Å/pix.
x 512 pix.
= 555.52 Å
1.09 Å/pix.
x 512 pix.
= 555.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.085 Å
Density
Contour LevelBy AUTHOR: 0.05 / Movie #1: 0.05
Minimum - Maximum-0.27577314 - 0.5731661
Average (Standard dev.)0.00049812626 (±0.01145673)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 555.52 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0851.0851.085
M x/y/z512512512
origin x/y/z0.0000.0000.000
length x/y/z555.520555.520555.520
α/β/γ90.00090.00090.000
start NX/NY/NZ111-94150
NX/NY/NZ111123111
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS512512512
D min/max/mean-0.2760.5730.000

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Supplemental data

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Segmentation: #1

Fileemd_10181_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire Rabbit 80S ribosome stalled on polyA mRNA

EntireName: Rabbit 80S ribosome stalled on polyA mRNA / Number of components: 91

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Component #1: protein, Rabbit 80S ribosome stalled on polyA mRNA

ProteinName: Rabbit 80S ribosome stalled on polyA mRNA / Recombinant expression: No
MassTheoretical: 4.5 MDa

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Component #2: protein, ribosome

ProteinName: ribosome / Recombinant expression: No
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #3: protein, polyA mRNA

ProteinName: polyA mRNA / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Salmonella virus SP6 (bacteriophage)

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Component #4: nucleic-acid, 18S ribosomal RNA

nucleic acidName: 18S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUGGUUCCUU UGGUCGCUCG CUCCUCUCCU ACUUGGAUAA CUGUGGUAAU UCUAGAGCUA AUACAUGCCG ACGGGCGCUG ACCCCCUUCG CGGGGGGGAU GCGUGCAUUU AUCAGAUCAA AACCAACCCG GUCAGCCCCU CUCCGGCCCC GGCCGGGGGG CGGGCGCCGG CGGCUUUGGU GACUCUAGAU AACCUCGGGC CGAUCGCACG CCCCCCGUGG CGGCGACGAC CCAUUCGAAC GUCUGCCCUA UCAACUUUCG AUGGUAGUCG CCGUGCCUAC CAUGGUGACC ACGGGUGACG GGGAAUCAGG GUUCGAUUCC GGAGAGGGAG CCUGAGAAAC GGCUACCACA UCCAAGGAAG GCAGCAGGCG CGCAAAUUAC CCACUCCCGA CCCGGGGAGG UAGUGACGAA AAAUAACAAU ACAGGACUCU UUCGAGGCCC UGUAAUUGGA AUGAGUCCAC UUUAAAUCCU UUAACGAGGA UCCAUUGGAG GGCAAGUCUG GUGCCAGCAG CCGCGGUAAU UCCAGCUCCA AUAGCGUAUA UUAAAGUUGC UGCAGUUAAA AAGCUCGUAG UUGGAUCUUG GGAGCGGGCG GGCGGUCCGC CGCGAGGCGA GCCACCGCCC GUCCCCGCCC CUUGCCUCUC GGCGCCCCCU CGAUGCUCUU AGCUGAGUGU CCCGCGGGGC CCGAAGCGUU UACUUUGAAA AAAUUAGAGU GUUCAAAGCA GGCCCGAGCC GCCUGGAUAC CGCAGCUAGG AAUAAUGGAA UAGGACCGCG GUUCUAUUUU GUUGGUUUUC GGAACUGAGG CCAUGAUUAA GAGGGACGGC CGGGGGCAUU CGUAUUGCGC CGCUAGAGGU GAAAUUCUUG GACCGGCGCA AGACGGACCA GAGCGAAAGC AUUUGCCAAG AAUGUUUUCA UUAAUCAAGA ACGAAAGUCG GAGGUUCGAA GACGAUCAGA UACCGUCGUA GUUCCGACCA UAAACGAUGC CGACCGGCGA UGCGGCGGCG UUAUUCCCAU GACCCGCCGG GCAGCUUCCG GGAAACCAAA GUCUUUGGGU UCCGGGGGGA GUAUGGUUGC AAAGCUGAAA CUUAAAGGAA UUGACGGAAG GGCACCACCA GGAGUGGAGC CUGCGGCUUA AUUUGACUCA ACACGGGAAA CCUCACCCGG CCCGGACACG GACAGGAUUG ACAGAUUGAU AGCUCUUUCU CGAUUCCGUG GGUGGUGGUG CAUGGCCGUU CUUAGUUGGU GGAGCGAUUU GUCUGGUUAA UUCCGAUAAC GAACGAGACU CUGGCAUGCU AACUAGUUAC GCGACCCCCG AGCGGUCGGC GUCCCCCAAC UUCUUAGAGG GACAAGUGGC GUUCAGCCAC CCGAGAUUGA GCAAUAACAG GUCUGUGAUG CCCUUAGAUG UCCGGGGCUG CACGCGCGCU ACACUGACUG GCUCAGCGUG UGCCUACCCU ACGCCGGCAG GCGCGGGUAA CCCGUUGAAC CCCAUUCGUG AUGGGGAUCG GGGAUUGCAA UUAUUCCCCA UGAACGAGGA AUUCCCAGUA AGUGCGGGUC AUAAGCUUGC GUUGAUUAAG UCCCUGCCCU UUGUACACAC CGCCCGUCGC UACUACCGAU UGGAUGGUUU AGUGAGGCCC UCGGAUCGGC CCCGCCGGGG UCGGCCCACG GCCCUGGCGG AGCGCUGAGA AGACGGUCGA ACUUGACUAU CUAGAGGAAG UAAAAGUCGU AACAAGGUUU CCGUAGGUGA ACCUGCGGAA GGAUCAUUA
MassTheoretical: 602.777875 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #5: protein, uS2

ProteinName: uS2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 32.958016 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #6: protein, 40S ribosomal protein S3a

ProteinName: 40S ribosomal protein S3a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 30.002061 kDa
SourceSpecies: Rabbit (rabbit)

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Component #7: protein, uS5

ProteinName: uS5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.327395 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #8: protein, uS5

ProteinName: uS5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.715344 kDa
SourceSpecies: Rabbit (rabbit)

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Component #9: protein, 40S ribosomal protein S4

ProteinName: 40S ribosomal protein S4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.654869 kDa
SourceSpecies: Rabbit (rabbit)

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Component #10: protein, Ribosomal protein S5

ProteinName: Ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.913453 kDa
SourceSpecies: Rabbit (rabbit)

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Component #11: protein, 40S ribosomal protein S6

ProteinName: 40S ribosomal protein S6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.751906 kDa
SourceSpecies: Rabbit (rabbit)

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Component #12: protein, 40S ribosomal protein S7

ProteinName: 40S ribosomal protein S7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.168914 kDa
SourceSpecies: Rabbit (rabbit)

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Component #13: protein, 40S ribosomal protein S8

ProteinName: 40S ribosomal protein S8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.263387 kDa
SourceSpecies: Rabbit (rabbit)

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Component #14: protein, Ribosomal protein S9 (Predicted)

ProteinName: Ribosomal protein S9 (Predicted)Ribosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.641564 kDa
SourceSpecies: Rabbit (rabbit)

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Component #15: protein, S10_plectin domain-ontaining protein

ProteinName: S10_plectin domain-ontaining protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.933846 kDa
SourceSpecies: Rabbit (rabbit)

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Component #16: protein, Ribosomal protein S11

ProteinName: Ribosomal protein S11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.468826 kDa
SourceSpecies: Rabbit (rabbit)

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Component #17: protein, 40S ribosomal protein S12

ProteinName: 40S ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.538987 kDa
SourceSpecies: Rabbit (rabbit)

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Component #18: protein, Ribosomal_S13_N domain-containing protein

ProteinName: Ribosomal_S13_N domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.259389 kDa
SourceSpecies: Rabbit (rabbit)

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Component #19: protein, uS11

ProteinName: uS11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.133984 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #20: protein, uS19

ProteinName: uS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.049182 kDa
SourceSpecies: Rabbit (rabbit)

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Component #21: protein, Ribosomal protein S16

ProteinName: Ribosomal protein S16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.477377 kDa
SourceSpecies: Rabbit (rabbit)

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Component #22: protein, eS17

ProteinName: eS17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.552119 kDa
SourceSpecies: Rabbit (rabbit)

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Component #23: protein, eS13

ProteinName: eS13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.759777 kDa
SourceSpecies: Rabbit (rabbit)

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Component #24: protein, eS19

ProteinName: eS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.10664 kDa
SourceSpecies: Rabbit (rabbit)

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Component #25: protein, Ribosomal_S10 domain-containing protein

ProteinName: Ribosomal_S10 domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.398763 kDa
SourceSpecies: Rabbit (rabbit)

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Component #26: protein, eS21

ProteinName: eS21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.124389 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #27: protein, Ribosomal protein S15a

ProteinName: Ribosomal protein S15aRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.865555 kDa
SourceSpecies: Rabbit (rabbit)

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Component #28: protein, uS12

ProteinName: uS12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.844666 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #29: protein, eS24

ProteinName: eS24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.107924 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #30: protein, eS25

ProteinName: eS25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.776224 kDa
SourceSpecies: Rabbit (rabbit)

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Component #31: protein, eS26

ProteinName: eS26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.047532 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #32: protein, 40S ribosomal protein S27

ProteinName: 40S ribosomal protein S27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.480186 kDa
SourceSpecies: Rabbit (rabbit)

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Component #33: protein, Ribosomal protein S28

ProteinName: Ribosomal protein S28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.855052 kDa
SourceSpecies: Rabbit (rabbit)

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Component #34: protein, uS14

ProteinName: uS14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.690821 kDa
SourceSpecies: Rabbit (rabbit)

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Component #35: protein, 40S ribosomal protein S30

ProteinName: 40S ribosomal protein S30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.498884 kDa
SourceSpecies: Rabbit (rabbit)

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Component #36: protein, Ribosomal protein S27a

ProteinName: Ribosomal protein S27aRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.004041 kDa
SourceSpecies: Rabbit (rabbit)

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Component #37: protein, WD_REPEATS_REGION domain-containing protein

ProteinName: WD_REPEATS_REGION domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.115652 kDa
SourceSpecies: Rabbit (rabbit)

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Component #38: nucleic-acid, polyA mRNA

nucleic acidName: polyA mRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAAAAAAAAA
MassTheoretical: 3.2471 kDa
SourceSpecies: Homo sapiens (human)

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Component #39: protein, Ribosomal protein L8

ProteinName: Ribosomal protein L8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.088863 kDa
SourceSpecies: Rabbit (rabbit)

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Component #40: protein, uL3

ProteinName: uL3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 46.107977 kDa
SourceSpecies: Rabbit (rabbit)

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Component #41: protein, uL4

ProteinName: uL4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 47.727559 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #42: protein, 60S ribosomal protein L5

ProteinName: 60S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.481828 kDa
SourceSpecies: Rabbit (rabbit)

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Component #43: protein, 60S ribosomal protein L6

ProteinName: 60S ribosomal protein L6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.028336 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #44: protein, uL30

ProteinName: uL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.201836 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #45: protein, eL8

ProteinName: eL8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 36.221516 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #46: protein, uL6

ProteinName: uL6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.871418 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #47: protein, 60S ribosomal protein L10

ProteinName: 60S ribosomal protein L10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.643057 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #48: protein, Ribosomal protein L11

ProteinName: Ribosomal protein L11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.288465 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #49: protein, eL13

ProteinName: eL13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.331723 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #50: protein, Ribosomal protein L14

ProteinName: Ribosomal protein L14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.870549 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #51: protein, Ribosomal protein L15

ProteinName: Ribosomal protein L15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.207285 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #52: protein, uL13

ProteinName: uL13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.533299 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #53: protein, uL22

ProteinName: uL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.444221 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #54: protein, eL18

ProteinName: eL18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.699688 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #55: protein, eL19

ProteinName: eL19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.535281 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #56: protein, eL20

ProteinName: eL20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.827561 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #57: protein, eL21

ProteinName: eL21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.609988 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #58: protein, eL22

ProteinName: eL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.813015 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #59: protein, Ribosomal protein L23

ProteinName: Ribosomal protein L23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.892505 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #60: protein, TRASH domain-containing protein

ProteinName: TRASH domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.825111 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #61: protein, Ribosomal_L23eN domain-containing protein

ProteinName: Ribosomal_L23eN domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.768246 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #62: protein, Ribosomal protein L26

ProteinName: Ribosomal protein L26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.303363 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #63: protein, 60S ribosomal protein L27

ProteinName: 60S ribosomal protein L27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.835831 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #64: protein, Ribosomal_L18e/L15P domain-containing protein

ProteinName: Ribosomal_L18e/L15P domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.620561 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #65: protein, eL29

ProteinName: eL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.708707 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #66: protein, Ribosomal_L7Ae domain-containing protein

ProteinName: Ribosomal_L7Ae domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.807065 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #67: protein, eL31

ProteinName: eL31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.494938 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #68: protein, eL32

ProteinName: eL32CD59 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.898932 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #69: protein, eL33

ProteinName: eL33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.580809 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #70: protein, eL34

ProteinName: eL34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.196894 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #71: protein, uL29

ProteinName: uL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.566599 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #72: protein, 60S ribosomal protein L36

ProteinName: 60S ribosomal protein L36 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.263834 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #73: protein, Ribosomal protein L37

ProteinName: Ribosomal protein L37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.111032 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #74: protein, eL38

ProteinName: eL38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.238948 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #75: protein, eL39

ProteinName: eL39 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.455775 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #76: protein, eL40

ProteinName: eL40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.69979 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #77: protein, 60s ribosomal protein l41

ProteinName: 60s ribosomal protein l41 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.473451 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #78: protein, eL42

ProteinName: eL42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.476973 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #79: protein, eL43

ProteinName: eL43 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.29935 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #80: protein, Ribosomal_L28e domain-containing protein

ProteinName: Ribosomal_L28e domain-containing protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.783614 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #81: protein, uL10

ProteinName: uL10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.380504 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #82: protein, Ribosomal protein L12

ProteinName: Ribosomal protein L12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.847619 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #83: nucleic-acid, 28S ribosomal RNA

nucleic acidName: 28S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAACG GCGAGUGAAC AGGGAAGAGC CCAGCGCCGA AUCCCCGCCC CGCGGCGGGG CGCGGGAAAU GUGGCGUACG GAAGACCCAC UCCCCGGCGC CGGGGGGCCC AAGUCCUUCU GAUCGAGGCC CAGCCCGUGG ACGGUGUGAG GCCGGUAGCG GCCCCCGGCG CGCCGGGCCC GGGUCUUCCC GGAGUCGGGU UGCUUGGGAA UGCAGCCCAA AGCGGGUGGU AAACUCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGAGAGUUC AAGAGGGCGU GAAACCGUUA AGAGGUAAAC GGGUGGGGUC CGCGCAGUCC GCCCGGAGGA UUCAACCCGG CGGCGCGGUC CGGCCGUGCC GGCGGGGCGG AUCUCCCGCU CCCGUCGGCG GGGGACCGCC CCCCGGCCGG CGACCGGCCG CCGCCGGGCG CAUUUCCACC GCGGCGGUGC GCCGCGACCG GCUCCGGGAC GGCUGGGAAG GCCCCGGCGG GGAAGGUGGC CCGGGGGCCC CGGGUGUUAC AGCCCCGCCC CGGCAGCAGC AGCUCGCCGA AUCCCGGGGC CGAGGGAGCG AGACCCGUGC GCCGCGCUCU CCCCCCUCGG GGGGGCCGGG CCACCCCUCC CACGGCGCGA CCGCUGGGGC GGACUGUCCA GUGCGCCCCG GGCGGGUCGC GCCGUCGGGC CCGGGGGGCC ACGCGUCCCC CGCGAGCGCA CGGGGUCGGC GGCGAUGUCG GCUACCCACC CGACCCGUCU UGAAACACGG ACCAAGGAGU CUAACACGUG CGCGAGUCGG GGGCUACGAA AGCCGCCGUG GCGCAAUGAA GGUGAAGGCC GGCGGCGCGC CGCCGGCCGA GGUGGGAUCC CGAGGCCUUC CAGUCCGCCG AGGGCGCACC ACCGGCCCGU CUCGCCCGCC GCGCCGGGGA GGUGGAGCAC GAGCGCACGU GUUAGGACCC GAAAGAUGGU GAACUAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCGAA UCGGUCGUCC GACCUGGGUA UAGGGGCGAA AGACUAAUCG AACCAUCUAG UAGCUGGUUC CCUCCAAAGU UUCCCUCAGG AUAGCUGGCG CUCUCCGCAG UUUUAUCCGG UAAAGCGAAU GAUUAGAGGU CUUGGGGCCG AAACGAUCUC AACCUAUUCU CAAACUUUAA AUGGGUAAGA AGCCCGGCUC GCUGGUGUGG AGCCGGGCGU GGAAUGCGAG UGCCUAGUGG GCCACUUUUG GUAAGCAGAA CUGGCGCUGC GGGAUGAACC GAACGCCGGG UUAAGGCGCC CGAUGCCGAC GCUCAUCAGA CCCCAGAAAA GGUGUUGGUU GAUAUAGACA GCAGGAUGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACCUGCCG AAUCAACUAG CCCUGAAAAU GGAUGGCGCU GGAGCGUCGG GCCCAUACCC GGCCGUCGCU GGCAGUCGGC AGAGCGAGAA AGAGAGUGGC GCGGACGCUA CGCCGCGACG AGUAGGAGGG CCGCUGCGGU GAGCCUUGAA GCCUAGGGCG CGGGCCCGGG UGGAGCCGCC GCAGGUGCAG AUCUUGGUGG UAGUAGCAAA UAUUCAAACG AGAACUUUGA AGGCCGAAGU GGAGAAGGGU UCCAUGUGAA CAGCAGUUGA ACAUGGGUCA GUCGGUCCUG AGAGAUGGGC GAGCGCCGUU CCGAAGGGAC GGGCGAUGGC CUCCGUUGCC CUCAGCCGAU CGAAAGGGAG UCGGGUUCAG AUCCCCGAAU CCGGAGUGGC GGAGAUGGGC GCCGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAGCCCCGG GGAGAGUUCU CUUUUCUUUG UGAAGGGCAG GGCGCCCUGG AAUGGGUUCG CCCCGAGAGA GGGGCCCGUG CCUUGGAAAG CGUCGCGGUU CCGGCGGCGU CCGGUGAGCU CUCGCUGGCC CUUGAAAAUC CGGGGGAGAG GGUGUAAAUC UCGCGCCGGG CCGUACCCAU AUCCGCAGCA GGUCUCCAAG GUGAACAGCC UCUGGCAUGU UGGAACAAUG UAGGUAAGGG AAGUCGGCAA GCCGGAUCCG UAACUUCGGG AUAAGGAUUG GCUCUAAGGG CUGGCAGCCG ACUUAGAACU GGUGCGGACC AGGGGAAUCC GACUGUUUAA UUAAAACAAA GCAUCGCGAA GGCCCGCGGC GGGUGUUGAC GCGAUGUGAU UUCUGCCCAG UGCUCUGAAU GUCAAAGUGA AGAAAUUCAA UGAAGCGCGG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUG AACGAGAUUC CCACUGUCCC UACCUACUAU CCAGCGAAAC CACAGCCAAG GGAACGGGCU UGGCGGAAUC AGCGGGGAAA GAAGACCCUG UUGAGCUUGA CUCUAGUCUG GCACGGUGAA GAGACAUGAG AGGUGUAGAA UAAGUGGGAG GCCCGGGCCG CCGGUGAAAU ACCACUACUC UGAUCGUUUU UUCACUGACC CGGUGAGGCG GGGGGGCGAG CCGGGCUCUC GCUUCUGGCG CCAAGCGCCC GGCCGGCCGG GCGCGACCCG CUCCGGGGAC AGUGCCAGGU GGGGAGUUUG ACUGGGGCGG UACACCUGUC AAACGGUAAC GCAGGUGUCC UAAGGCGAGC UCAGGGAGGA CAGAAACCUC CCGUGGAGCA GAAGGGCAAA AGCUCGCUUG AUCUUGAUUU UCAGUACGAA UACAGACCGU GAAAGCGGGG CCUCACGAUC CUUCUGACCU UUUGGGUUUU AAGCAGGAGG UGUCAGAAAA GUUACCACAG GGAUAACUGG CUUGUGGCGG CCAAGCGUUC AUAGCGACGU CGCUUUUUGA UCCUUCGAUG UCGGCUCUUC CUAUCAUUGU GAAGCAGAAU UCACCAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACCGUCG UGAGACAGGU UAGUUUUACC CUACUGAUGA UGUGUUGUUG CCAUGGUAAU CCUGCUCAGU ACGAGAGGAA CCGCAGGUUC AGACAUUUGG UGUAUGUGCU UGGCUGAGGA GCCAAUGGGG CGAAGCUACC AUCUGUGGGA UUAUGACUGA ACGCCUCUAA GUCAGAAUCC CGCCCAGGCG GAACGAUAGC GCCGGAGCCU CGGUUGGCCU CGGAUAGCCG GUCCCCCGCC GGGGUCCGGU GCGGAGAGCC CUUCGUCCCG GGAAACGGGG CGGCCGGAAA GGGGGCCGCC CUCUCGCCCG UCACGCACCG CACGUUCGUG GGGAACCUGG CGCUAAACCA UUCGUAGACG ACCUGCUUCU GGGUCGGGGU UUCGUACGUA GCAGAGCAGC UGCUGCGAUC UAUUGAAAGU CAGCCCUCGA CACAAGGGUU UGU
MassTheoretical: 1.167622625 MDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #84: nucleic-acid, 5S ribosomal RNA

nucleic acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAGA CCGCCUGGGA AUACCGGGUG CUGUAGGCUU
MassTheoretical: 38.691914 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #85: nucleic-acid, 5.8S ribosomal RNA

nucleic acidName: 5.8S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAUC AUCGACACUU CGAACGCACU UGCGGCCCCG GGUUCCUCCC GGGGCUACGC CUGUCUGAGC GUCGCU
MassTheoretical: 50.143648 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #86: protein, poly-lysine nascent chain

ProteinName: poly-lysine nascent chain / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.084892 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #87: protein, Ribosomal protein

ProteinName: Ribosomal protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.879422 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #88: nucleic-acid, tRNA (Lys3)

nucleic acidName: tRNA (Lys3) / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCCCGCAUAG CUCAGCCGG(H2U) AGAGCA(PSU)CAG ACU(70U)UU(12A)A(PSU)C UGAGG(7MG)(H2U)(5MC)(5MC)A GGG(2MU)(PSU)CA(1MA)GU CCCUGUUCGG GCGCCA
MassTheoretical: 24.776012 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #89: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 275 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

+
Component #90: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 8 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

+
Component #91: ligand, SPERMIDINE

LigandName: SPERMIDINE / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 0.145246 kDa

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.4
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 41.8 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON III (4k x 4k)

-
Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 148615
3D reconstructionSoftware: RELION / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

-
Atomic model buiding

Modeling #1Refinement protocol: flexible / Refinement space: REAL
Output model

+
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