|Entry||Database: EMDB / ID: EMD-3945|
|Title||BK polyomavirus + heparin|
|Biological species||BK polyomavirus|
|Method||single particle reconstruction / cryo EM / Resolution: 3.6 Å|
|Authors||Hurdiss DL / Ranson NA|
|Citation||Journal: Structure / Year: 2018|
Title: The Structure of an Infectious Human Polyomavirus and Its Interactions with Cellular Receptors.
Authors: Daniel L Hurdiss / Martin Frank / Joseph S Snowden / Andrew Macdonald / Neil A Ranson /
Abstract: BK polyomavirus (BKV) causes polyomavirus-associated nephropathy and hemorrhagic cystitis in immunosuppressed patients. These are diseases for which we currently have limited treatment options, but ...BK polyomavirus (BKV) causes polyomavirus-associated nephropathy and hemorrhagic cystitis in immunosuppressed patients. These are diseases for which we currently have limited treatment options, but potential therapies could include pre-transplant vaccination with a multivalent BKV vaccine or therapeutics which inhibit capsid assembly or block attachment and entry into target cells. A useful tool in such efforts would be a high-resolution structure of the infectious BKV virion and how this interacts with its full repertoire of cellular receptors. We present the 3.4-Å cryoelectron microscopy structure of native, infectious BKV in complex with the receptor fragment of GT1b ganglioside. We also present structural evidence that BKV can utilize glycosaminoglycans as attachment receptors. This work highlights features that underpin capsid stability and provides a platform for rational design and development of urgently needed pharmacological interventions for BKV-associated diseases.
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_3945.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.0651 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Additional map: BK polyomavirus in complex with heparin (unsharpened map).
-Entire BK polyomavirus
|Entire||Name: BK polyomavirus / Number of components: 1|
-Component #1: virus, BK polyomavirus
|Virus||Name: BK polyomavirusBK virus / Class: VIRION / Empty: No / Enveloped: No / Isolate: STRAIN|
|Species||Species: BK polyomavirus / Strain: Dunlop|
|Source (natural)||Host Species: Homo sapiens (human)|
|Shell #1||Name of element: Icosahedron / Diameter: 500.0 Å / T number (triangulation number): 7|
|Specimen||Specimen state: Particle / Method: cryo EM|
|Sample solution||Specimen conc.: 0.25 mg/mL / pH: 7.5|
|Vitrification||Cryogen name: ETHANE|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 55 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: FEI FALCON III (4k x 4k)|
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