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基本情報
登録情報 | データベース: EMDB / ID: EMD-30710 | |||||||||
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タイトル | Masked wing-b region of TSC complex | |||||||||
![]() | masked wing-b region of TSC complex | |||||||||
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機能・相同性 | ![]() memory T cell differentiation / : / TSC1-TSC2 complex / Inhibition of TSC complex formation by PKB / regulation of phosphoprotein phosphatase activity / cellular response to decreased oxygen levels / regulation of insulin receptor signaling pathway / negative regulation of cilium assembly / regulation of cell-matrix adhesion / ATPase inhibitor activity ...memory T cell differentiation / : / TSC1-TSC2 complex / Inhibition of TSC complex formation by PKB / regulation of phosphoprotein phosphatase activity / cellular response to decreased oxygen levels / regulation of insulin receptor signaling pathway / negative regulation of cilium assembly / regulation of cell-matrix adhesion / ATPase inhibitor activity / cardiac muscle cell differentiation / negative regulation of ATP-dependent activity / Energy dependent regulation of mTOR by LKB1-AMPK / cell projection organization / negative regulation of cell size / response to growth factor / regulation of stress fiber assembly / activation of GTPase activity / protein folding chaperone complex / anoikis / negative regulation of TOR signaling / TBC/RABGAPs / negative regulation of mitophagy / regulation of small GTPase mediated signal transduction / AKT phosphorylates targets in the cytosol / Macroautophagy / negative regulation of macroautophagy / positive chemotaxis / negative regulation of Wnt signaling pathway / glucose import / Constitutive Signaling by AKT1 E17K in Cancer / positive regulation of focal adhesion assembly / associative learning / positive regulation of macroautophagy / regulation of endocytosis / phosphatase binding / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / ribosomal subunit export from nucleus / vesicle-mediated transport / negative regulation of TORC1 signaling / Hsp70 protein binding / myelination / negative regulation of insulin receptor signaling pathway / viral process / cellular response to starvation / lipid droplet / adult locomotory behavior / phosphatidylinositol 3-kinase/protein kinase B signal transduction / GTPase activator activity / cell-matrix adhesion / insulin-like growth factor receptor signaling pathway / ciliary basal body / positive regulation of GTPase activity / positive regulation of protein ubiquitination / kidney development / neural tube closure / hippocampus development / TP53 Regulates Metabolic Genes / synapse organization / negative regulation of protein kinase activity / Hsp90 protein binding / potassium ion transport / response to insulin / protein localization / cerebral cortex development / small GTPase binding / endocytosis / protein import into nucleus / lamellipodium / regulation of translation / heart development / cell cortex / protein-folding chaperone binding / cytoplasmic vesicle / adaptive immune response / cell population proliferation / postsynaptic density / lysosome / protein stabilization / negative regulation of translation / regulation of cell cycle / negative regulation of cell population proliferation / lysosomal membrane / perinuclear region of cytoplasm / Golgi apparatus / protein homodimerization activity / protein-containing complex / membrane / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.85 Å | |||||||||
![]() | Yang H / Yu Z / Chen X / Li J / Li N / Cheng J / Gao N / Yuan H / Ye D / Guan K / Xu Y | |||||||||
![]() | ![]() タイトル: Structural insights into TSC complex assembly and GAP activity on Rheb. 著者: Huirong Yang / Zishuo Yu / Xizi Chen / Jiabei Li / Ningning Li / Jiaxuan Cheng / Ning Gao / Hai-Xin Yuan / Dan Ye / Kun-Liang Guan / Yanhui Xu / ![]() ![]() 要旨: Tuberous sclerosis complex (TSC) integrates upstream stimuli and regulates cell growth by controlling the activity of mTORC1. TSC complex functions as a GTPase-activating protein (GAP) towards small ...Tuberous sclerosis complex (TSC) integrates upstream stimuli and regulates cell growth by controlling the activity of mTORC1. TSC complex functions as a GTPase-activating protein (GAP) towards small GTPase Rheb and inhibits Rheb-mediated activation of mTORC1. Mutations in TSC genes cause tuberous sclerosis. In this study, the near-atomic resolution structure of human TSC complex reveals an arch-shaped architecture, with a 2:2:1 stoichiometry of TSC1, TSC2, and TBC1D7. This asymmetric complex consists of two interweaved TSC1 coiled-coil and one TBC1D7 that spans over the tail-to-tail TSC2 dimer. The two TSC2 GAP domains are symmetrically cradled within the core module formed by TSC2 dimerization domain and central coiled-coil of TSC1. Structural and biochemical analyses reveal TSC2 GAP-Rheb complimentary interactions and suggest a catalytic mechanism, by which an asparagine thumb (N1643) stabilizes γ-phosphate of GTP and accelerate GTP hydrolysis of Rheb. Our study reveals mechanisms of TSC complex assembly and GAP activity. | |||||||||
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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マップデータ | ![]() | 500.1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 8.2 KB 8.2 KB | 表示 表示 | ![]() |
画像 | ![]() | 27.5 KB | ||
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文書・要旨 | ![]() | 315.7 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 315.3 KB | 表示 | |
XML形式データ | ![]() | 7.3 KB | 表示 | |
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EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | masked wing-b region of TSC complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.356 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
-全体 : Masked wing-b region of TSC complex
全体 | 名称: Masked wing-b region of TSC complex |
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要素 |
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-超分子 #1: Masked wing-b region of TSC complex
超分子 | 名称: Masked wing-b region of TSC complex / タイプ: complex / ID: 1 / 親要素: 0 |
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由来(天然) | 生物種: ![]() |
組換発現 | 生物種: ![]() |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.85 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 131022 |
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初期 角度割当 | タイプ: OTHER |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |