National Natural Science Foundation of China (NSFC)
31570736
中国
引用
ジャーナル: PLoS Pathog / 年: 2021 タイトル: Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission. 著者: Qianqian Shao / Xudong Jia / Yuanzhu Gao / Zhe Liu / Huan Zhang / Qiqi Tan / Xin Zhang / Huiqiong Zhou / Yinyin Li / De Wu / Qinfen Zhang / 要旨: Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like ...Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like viruses, with features similar to those of the Totiviridae family. Most reported members of this family infect fungi or protozoans and lack an extracellular life cycle stage. Here, we identified a new strain of OmRV and determined high-resolution structures for this virus using single-particle cryo-electron microscopy. The structures feature an unexpected protrusion at the five-fold vertex of the capsid. Disassociation of the protrusion could result in several conformational changes in the major capsid. All these structures, together with some biological results, suggest the protrusions' associations with the extracellular transmission of OmRV.
ダウンロード / ファイル: emd_30507.map.gz / 形式: CCP4 / 大きさ: 3.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
ボクセルのサイズ
X=Y=Z: 1.09 Å
密度
表面レベル
登録者による: 3.5 / ムービー #1: 3.5
最小 - 最大
-10.989091 - 13.088528
平均 (標準偏差)
-0.000000003864251 (±1.0)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-50
-50
180
サイズ
100
100
100
Spacing
100
100
100
セル
A=B=C: 109.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.09
1.09
1.09
M x/y/z
100
100
100
origin x/y/z
0.000
0.000
0.000
length x/y/z
109.000
109.000
109.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
64
53
65
NX/NY/NZ
139
143
124
MAP C/R/S
1
2
3
start NC/NR/NS
-50
-50
180
NC/NR/NS
100
100
100
D min/max/mean
-10.989
13.089
-0.000
-
添付データ
-
試料の構成要素
-
全体 : Omono River virus
全体
名称: Omono River virus (ウイルス)
要素
ウイルス: Omono River virus (ウイルス)
タンパク質・ペプチド: Capsid protein
-
超分子 #1: Omono River virus
超分子
名称: Omono River virus / タイプ: virus / ID: 1 / 親要素: 0 / 含まれる分子: all 詳細: Protrusion structure located upon the five-fold vertex of the major capsid. NCBI-ID: 753758 / 生物種: Omono River virus / Sci species strain: LZ / ウイルスタイプ: VIRION / ウイルス・単離状態: STRAIN / ウイルス・エンベロープ: No / ウイルス・中空状態: No
宿主
生物種: Culex quinquefasciatus (カ)
ウイルス殻
Shell ID: 1 / 名称: Major capsid / 直径: 450.0 Å / T番号(三角分割数): 1