National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
DK075037
米国
Japan Society for the Promotion of Science (JSPS)
JP16H06307
日本
Japan Society for the Promotion of Science (JSPS)
JP21H03598
日本
引用
ジャーナル: Nature / 年: 2023 タイトル: Lesion recognition by XPC, TFIIH and XPA in DNA excision repair. 著者: Jinseok Kim / Chia-Lung Li / Xuemin Chen / Yanxiang Cui / Filip M Golebiowski / Huaibin Wang / Fumio Hanaoka / Kaoru Sugasawa / Wei Yang / 要旨: Nucleotide excision repair removes DNA lesions caused by ultraviolet light, cisplatin-like compounds and bulky adducts. After initial recognition by XPC in global genome repair or a stalled RNA ...Nucleotide excision repair removes DNA lesions caused by ultraviolet light, cisplatin-like compounds and bulky adducts. After initial recognition by XPC in global genome repair or a stalled RNA polymerase in transcription-coupled repair, damaged DNA is transferred to the seven-subunit TFIIH core complex (Core7) for verification and dual incisions by the XPF and XPG nucleases. Structures capturing lesion recognition by the yeast XPC homologue Rad4 and TFIIH in transcription initiation or DNA repair have been separately reported. How two different lesion recognition pathways converge and how the XPB and XPD helicases of Core7 move the DNA lesion for verification are unclear. Here we report on structures revealing DNA lesion recognition by human XPC and DNA lesion hand-off from XPC to Core7 and XPA. XPA, which binds between XPB and XPD, kinks the DNA duplex and shifts XPC and the DNA lesion by nearly a helical turn relative to Core7. The DNA lesion is thus positioned outside of Core7, as would occur with RNA polymerase. XPB and XPD, which track the lesion-containing strand but translocate DNA in opposite directions, push and pull the lesion-containing strand into XPD for verification.
分子 #8: DNA repair protein complementing XP-C cells
分子
名称: DNA repair protein complementing XP-C cells / タイプ: protein_or_peptide / ID: 8 / 光学異性体: LEVO
由来(天然)
生物種: Homo sapiens (ヒト)
組換発現
生物種: Spodoptera frugiperda (ツマジロクサヨトウ)
配列
文字列: M D Y K D D D D K H M A R K R A A G G E P R G R E L R S Q K S K A K S K A R R E E E E E D A F E D E K P P K K S L L S K V S Q G K R K R G C S H P G G S A D G P A K K K V A ...文字列:
M D Y K D D D D K H M A R K R A A G G E P R G R E L R S Q K S K A K S K A R R E E E E E D A F E D E K P P K K S L L S K V S Q G K R K R G C S H P G G S A D G P A K K K V A K V T V K S E N L K V I K D E A L S D G D D L R D F P S D L K K A H H L K R G A T M N E D S N E E E E E S E N D W E E V E E L S E P V L G D V R E S T A F S R S L L P V K P V E I E I E T P E Q A K T R E R S E K I K L E F E T Y L R R A M K R F N K G V H E D T H K V H L L C L L A N G F Y R N N I C S Q P D L H A I G L S I I P A R F T R V L P R D V D T Y Y L S N L V K W F I G T F T V N A E L S A S E Q D N L Q T T L E R R F A I Y S A R D D E E L V H I F L L I L R A L Q L L T R L V L S L Q P I P L K S A T A K G K K P S K E R L T A D P G G S S E T S S Q V L E N H T K P K T S K G T K Q E E T F A K G T C R P S A K G K R N K G G R K K R S K P S S S E E D E G P G D K Q E K A T Q R R P H G R E R R V A S R V S Y K E E S G S D E A G S G S D F E L S S G E A S D P S D E D S E P G P P K Q R K A P A P Q R T K A G S K S A S R T H R G S H R K D P S L P V A S S S S S S S K R G K K M C S D G E K A E K R S I A G I D Q W L E V F C E Q E E K W V C V D C V H G V V G Q P L T C Y K Y A T K P M T Y V V G I D S D G W V R D V T Q R Y D P V W M T V T R K C R V D A E W W A E T L R P Y Q S P F M D R E K K E D L E F Q A K H M D Q P L P T A I G L Y K N H P L Y A L K R H L L K Y E A I Y P E T A A I L G Y C R G E A V Y S R D C V H T L H S R D T W L K K A R V V R L G E V P Y K M V K G F S N R A R K A R L A E P Q L R E E N D L G L F G Y W Q T E E Y Q P P V A V D G K V P R N E F G N V Y L F L P S M M P I G C V Q L N L P N L H R V A R K L D I D C V Q A I T G F D F H G G Y S H P V T D G Y I V C E E F K D V L L T A W E N E Q A V I E R K E K E K K E K R A L G N W K L L A K G L L I R E R L K R R Y G P K S E A A A P H T D A G G G L S S D E E E G T S S Q A E A A R I L A A S W P Q N R E D E E K Q K L K G G P K K T K R E K K A A A S H L F P F E K L
+
分子 #9: UV excision repair protein RAD23 homolog B
分子
名称: UV excision repair protein RAD23 homolog B / タイプ: protein_or_peptide / ID: 9 / 光学異性体: LEVO
由来(天然)
生物種: Homo sapiens (ヒト)
組換発現
生物種: Spodoptera frugiperda (ツマジロクサヨトウ)
配列
文字列: M Q V T L K T L Q Q Q T F K I D I D P E E T V K A L K E K I E S E K G K D A F P V A G Q K L I Y A G K I L N D D T A L K E Y K I D E K N F V V V M V T K P K A V S T P A P A ...文字列:
M Q V T L K T L Q Q Q T F K I D I D P E E T V K A L K E K I E S E K G K D A F P V A G Q K L I Y A G K I L N D D T A L K E Y K I D E K N F V V V M V T K P K A V S T P A P A T T Q Q S A P A S T T A V T S S T T T T V A Q A P T P V P A L A P T S T P A S I T P A S A T A S S E P A P A S A A K Q E K P A E K P A E T P V A T S P T A T D S T S G D S S R S N L F E D A T S A L V T G Q S Y E N M V T E I M S M G Y E R E Q V I A A L R A S F N N P D R A V E Y L L M G I P G D R E S Q A V V D P P Q A A S T G A P Q S S A V A A A A A T T T A T T T T T S S G G H P L E F L R N Q P Q F Q Q M R Q I I Q Q N P S L L P A L L Q Q I G R E N P Q L L Q Q I S Q H Q E H F I Q M L N E P V Q E A G G Q G G G G G G G S G G I A E A G S G H M N Y I Q V T P Q E K E A I E R L K A L G F P E G L V I Q A Y F A C E K N E N L A A N F L L Q Q N F D E D L E H H H H H H