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- EMDB-29674: Initial DNA-lesion (Cy5) binding by XPC and TFIIH focused -

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Basic information

Entry
Database: EMDB / ID: EMD-29674
TitleInitial DNA-lesion (Cy5) binding by XPC and TFIIH focused
Map data
Sample
  • Complex: bulky DNA lesion recognition complex 1
    • Protein or peptide: x 10 types
    • DNA: x 2 types
Biological speciesHomo sapiens (human) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.2 Å
AuthorsKim J / Yang W
Funding support United States, Japan, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)DK075037 United States
Japan Society for the Promotion of Science (JSPS)JP16H06307 Japan
Japan Society for the Promotion of Science (JSPS)JP21H03598 Japan
CitationJournal: Nature / Year: 2023
Title: Lesion recognition by XPC, TFIIH and XPA in DNA excision repair.
Authors: Jinseok Kim / Chia-Lung Li / Xuemin Chen / Yanxiang Cui / Filip M Golebiowski / Huaibin Wang / Fumio Hanaoka / Kaoru Sugasawa / Wei Yang /
Abstract: Nucleotide excision repair removes DNA lesions caused by ultraviolet light, cisplatin-like compounds and bulky adducts. After initial recognition by XPC in global genome repair or a stalled RNA ...Nucleotide excision repair removes DNA lesions caused by ultraviolet light, cisplatin-like compounds and bulky adducts. After initial recognition by XPC in global genome repair or a stalled RNA polymerase in transcription-coupled repair, damaged DNA is transferred to the seven-subunit TFIIH core complex (Core7) for verification and dual incisions by the XPF and XPG nucleases. Structures capturing lesion recognition by the yeast XPC homologue Rad4 and TFIIH in transcription initiation or DNA repair have been separately reported. How two different lesion recognition pathways converge and how the XPB and XPD helicases of Core7 move the DNA lesion for verification are unclear. Here we report on structures revealing DNA lesion recognition by human XPC and DNA lesion hand-off from XPC to Core7 and XPA. XPA, which binds between XPB and XPD, kinks the DNA duplex and shifts XPC and the DNA lesion by nearly a helical turn relative to Core7. The DNA lesion is thus positioned outside of Core7, as would occur with RNA polymerase. XPB and XPD, which track the lesion-containing strand but translocate DNA in opposite directions, push and pull the lesion-containing strand into XPD for verification.
History
DepositionFeb 2, 2023-
Header (metadata) releaseMay 3, 2023-
Map releaseMay 3, 2023-
UpdateMay 17, 2023-
Current statusMay 17, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_29674.png

Downloads & links

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Map

FileDownload / File: emd_29674.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 384 pix.
= 319.872 Å		0.83 Å/pix.
x 384 pix.
= 319.872 Å		0.83 Å/pix.
x 384 pix.
= 319.872 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.833 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.005
Minimum - Maximum-0.011362825 - 0.02741581
Average (Standard dev.)9.7724704e-05 (±0.00090234325)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 319.872 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_29674_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_29674_half_map_1.map
Projections & Slices
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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_29674_half_map_2.map
Projections & Slices
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Sample components

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Entire : bulky DNA lesion recognition complex 1

EntireName: bulky DNA lesion recognition complex 1
Components
  • Complex: bulky DNA lesion recognition complex 1
    • Protein or peptide: General transcription and DNA repair factor IIH helicase subunit XPB
    • Protein or peptide: TFIIH basal transcription factor complex helicase XPD subunit
    • Protein or peptide: General transcription factor IIH subunit 1
    • Protein or peptide: General transcription factor IIH subunit 4
    • Protein or peptide: General transcription factor IIH subunit 2
    • Protein or peptide: General transcription factor IIH subunit 3
    • Protein or peptide: General transcription factor IIH subunit 5
    • Protein or peptide: DNA repair protein complementing XP-C cells
    • Protein or peptide: UV excision repair protein RAD23 homolog B
    • Protein or peptide: Centrin-2
    • DNA: DNA1
    • DNA: DNA2

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Supramolecule #1: bulky DNA lesion recognition complex 1

SupramoleculeName: bulky DNA lesion recognition complex 1 / type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 270 KDa

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Macromolecule #1: General transcription and DNA repair factor IIH helicase subunit XPB

MacromoleculeName: General transcription and DNA repair factor IIH helicase subunit XPB
type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: mgkrdradrd kkksrkrhy e deeddeed ap gndpqea vps aagkqv desg tkvde ygakd yrlq mplkdd hts rplwvap dg hifleafs p vykyaqdfl vaiaepvcrp thvheyklt a yslyaavs vg lqtsdit eyl rklskt gvpd gimqf ...String:
mgkrdradrd kkksrkrhy e deeddeed ap gndpqea vps aagkqv desg tkvde ygakd yrlq mplkdd hts rplwvap dg hifleafs p vykyaqdfl vaiaepvcrp thvheyklt a yslyaavs vg lqtsdit eyl rklskt gvpd gimqf iklct vsyg kvklvl khn ryfvesc hp dviqhllq d pvirecrlr nsegeateli tetftsksa i sktaessg gp stsrvtd pqg ksdipm dlfd fyeqm dkdee eeee tqtvsf evk qemieel qk rcihleyp l laeydfrnd svnpdinidl kptavlrpy q ekslrkmf gn grarsgv ivl pcgagk slvg vtaac tvrkr clvl gnsavs veq wkaqfkm ws tiddsqic r ftsdakdkp igcsvaisty smlghttkr s weaervme wl ktqewgl mil devhti pakm frrvl tivqa hckl gltatl vre ddkivdl nf ligpklye a nwmelqnng yiakvqcaev wcpmspefy r eyvaiktk kr illytmn pnk fracqf likf herrn dkiiv fadn vfalke yai rlnkpyi yg ptsqgerm q ilqnfkhnp kintifiskv gdtsfdlpe a nvliqiss hg gsrrqea qrl grvlra kkgm vaeey naffy slvs qdtqem ays tkrqrfl vd qgysfkvi t klagmeeed lafstkeeqq qllqkvlaa t dldaeeev va gefgsrs sqa srrfgt mssm sgadd tvyme yhss rskaps khv hplfkrf rk

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Macromolecule #2: TFIIH basal transcription factor complex helicase XPD subunit

MacromoleculeName: TFIIH basal transcription factor complex helicase XPD subunit
type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: mklnvdgllv yfpydyiype qfsymrelkr tldakghgvl empsgtgktv sllalimayq rayplevtk liycsrtvpe iekvieelrk llnfyekqeg eklpflglal ssrknlcihp e vtplrfgk dvdgkchslt asyvraqyqh dtslphcrfy eefdahgrev ...String:
mklnvdgllv yfpydyiype qfsymrelkr tldakghgvl empsgtgktv sllalimayq rayplevtk liycsrtvpe iekvieelrk llnfyekqeg eklpflglal ssrknlcihp e vtplrfgk dvdgkchslt asyvraqyqh dtslphcrfy eefdahgrev plpagiynld dl kalgrrq gwcpyflary silhanvvvy syhylldpki adlvskelar kavvvfdeah nid nvcids msvnltrrtl drcqgnletl qktvlriket deqrlrdeyr rlveglreas aare tdahl anpvlpdevl qeavpgsirt aehflgflrr lleyvkwrlr vqhvvqespp aflsg laqr vciqrkplrf caerlrsllh tleitdladf spltllanfa tlvstyakgf tiiiep fdd rtptianpil hfscmdasla ikpvferfqs viitsgtlsp ldiypkildf hpvtmat ft mtlarvclcp miigrgndqv aisskfetre diavirnygn lllemsavvp dgivafft s yqymestvas wyeqgileni qrnkllfiet qdgaetsval ekyqeaceng rgaillsva rgkvsegidf vhhygravim fgvpyvytqs rilkarleyl rdqfqirend fltfdamrha aqcvgrair gktdyglmvf adkrfargdk rgklprwiqe hltdanlnlt vdegvqvaky f lrqmaqpf hredqlglsl lsleqlesee tlkrieqiaq qlDYKDDDDK

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Macromolecule #3: General transcription factor IIH subunit 1

MacromoleculeName: General transcription factor IIH subunit 1 / type: protein_or_peptide / ID: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: matsseevll ivkkvrqkkq dgalylmaer iawapegkdr ftishmyadi kcqkispegk akiqlqlvl hagdttnfhf snestavker davkdllqql lpkfkrkank eleeknrmlq e dpvlfqly kdlvvsqvis aeefwanrln vnatdsssts nhkqdvgisa ...String:
matsseevll ivkkvrqkkq dgalylmaer iawapegkdr ftishmyadi kcqkispegk akiqlqlvl hagdttnfhf snestavker davkdllqql lpkfkrkank eleeknrmlq e dpvlfqly kdlvvsqvis aeefwanrln vnatdsssts nhkqdvgisa afladvrpqt dg cnglryn ltsdiiesif rtypavkmky aenvphnmte kefwtrffqs hyfhrdrlnt gsk dlfaec akidekglkt mvslgvknpl ldltaledkp ldegygissv psasnsksik ensn aaiik rfnhhsamvl aaglrkqeaq neqtsepsnm dgnsgdadcf qpavkraklq esiey edlg knnsvktial nlkksdryyh gptpiqslqy atsqdiinsf qsirqemeay tpkltq vls ssaasstita lspggalmqg gtqqainqmv pndiqselkh lyvavgellr hfwscfp vn tpfleekvvk mksnlerfqv tklcpfqeki rrqylstnlv shieemlqta ynklhtwq s rrlmkkt

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Macromolecule #4: General transcription factor IIH subunit 4

MacromoleculeName: General transcription factor IIH subunit 4 / type: protein_or_peptide / ID: 4 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: mestpsrgln rvhlqcrnlq eflgglspgv ldrlyghpat clavfrelps laknwvmrml fleqplpqa avalwvkkef skaqeestgl lsglriwhtq llpgglqgli lnpifrqnlr i allgggka wsddtsqlgp dkhardvpsl dkyaeerwev vlhfmvgsps ...String:
mestpsrgln rvhlqcrnlq eflgglspgv ldrlyghpat clavfrelps laknwvmrml fleqplpqa avalwvkkef skaqeestgl lsglriwhtq llpgglqgli lnpifrqnlr i allgggka wsddtsqlgp dkhardvpsl dkyaeerwev vlhfmvgsps aavsqdlaql ls qaglmks tepgeppcit sagfqfllld tpaqlwyfml qylqtaqsrg mdlveilsfl fql sfstlg kdysvegmsd sllnflqhlr efglvfqrkr ksrryyptrl ainlssgvsg aggt vhqpg fivvetnyrl yayteselqi alialfseml yrfpnmvvaq vtresvqqai asgit aqqi ihflrtrahp vmlkqtpvlp ptitdqirlw elerdrlrft egvlynqfls qvdfel lla harelgvlvf ensakrlmvv tpaghsdvkr fwkrqkhss

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Macromolecule #5: General transcription factor IIH subunit 2

MacromoleculeName: General transcription factor IIH subunit 2 / type: protein_or_peptide / ID: 5 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGSSHHHHHH SSGLEVLFQG PHMDEEPERT KRWEGGYERT WEILKEDESG SLKATIEDIL FKAKRKRVFE HHGQVRLGMM RHLYVVVDGS RTMEDQDLKP NRLTCTLKLL EYFVEEYFDQ NPISQIGIIV TKSKRAEKLT ELSGNPRKHI TSLKKAVDMT CHGEPSLYNS ...String:
MGSSHHHHHH SSGLEVLFQG PHMDEEPERT KRWEGGYERT WEILKEDESG SLKATIEDIL FKAKRKRVFE HHGQVRLGMM RHLYVVVDGS RTMEDQDLKP NRLTCTLKLL EYFVEEYFDQ NPISQIGIIV TKSKRAEKLT ELSGNPRKHI TSLKKAVDMT CHGEPSLYNS LSIAMQTLKH MPGHTSREVL IIFSSLTTCD PSNIYDLIKT LKAAKIRVSV IGLSAEVRVC TVLARETGGT YHVILDESHY KELLTHHVSP PPASSSSECS LIRMGFPQHT IASLSDQDAK PSFSMAHLDG NTEPGLTLGG YFCPQCRAKY CELPVECKIC GLTLVSAPHL ARSYHHLFPL DAFQEIPLEE YNGERFCYGC QGELKDQHVY VCAVCQNVFC VDCDVFVHDS LHCCPGCIHK IPAPSGV

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Macromolecule #6: General transcription factor IIH subunit 3

MacromoleculeName: General transcription factor IIH subunit 3 / type: protein_or_peptide / ID: 6 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: mvsdedelnl lvivvdanpi wwgkqalkes qftlskcida vmvlgnshlf mnrsnklavi ashiqesrf lypgkngrlg dffgdpgnpp efnpsgskdg kyelltsane viveeikdlm t ksdikgqh tetllagsla kalcyihrmn kevkdnqemk srilvikaae ...String:
mvsdedelnl lvivvdanpi wwgkqalkes qftlskcida vmvlgnshlf mnrsnklavi ashiqesrf lypgkngrlg dffgdpgnpp efnpsgskdg kyelltsane viveeikdlm t ksdikgqh tetllagsla kalcyihrmn kevkdnqemk srilvikaae dsalqymnfm nv ifaaqkq nilidacvld sdsgllqqac ditgglylkv pqmpsllqyl lwvflpdqdq rsq lilppp vhvdyraacf chrnlieigy vcsvclsifc nfspicttce tafkislppv lkak kkklk vsa

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Macromolecule #7: General transcription factor IIH subunit 5

MacromoleculeName: General transcription factor IIH subunit 5 / type: protein_or_peptide / ID: 7 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString:
mvnvlkgvli ecdpamkqfl lyldesnalg kkfiiqdidd thvfviaelv nvlqervgel mdqnafslt qk

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Macromolecule #8: DNA repair protein complementing XP-C cells

MacromoleculeName: DNA repair protein complementing XP-C cells / type: protein_or_peptide / ID: 8 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: M D Y K D D D D K H M A R K R A A G G E P R G R E L R S Q K S K A K S K A R R E E E E E D A F E D E K P P K K S L L S K V S Q G K R K R G C S H P G G S A D G P A K K K V A K ...String:
M D Y K D D D D K H M A R K R A A G G E P R G R E L R S Q K S K A K S K A R R E E E E E D A F E D E K P P K K S L L S K V S Q G K R K R G C S H P G G S A D G P A K K K V A K V T V K S E N L K V I K D E A L S D G D D L R D F P S D L K K A H H L K R G A T M N E D S N E E E E E S E N D W E E V E E L S E P V L G D V R E S T A F S R S L L P V K P V E I E I E T P E Q A K T R E R S E K I K L E F E T Y L R R A M K R F N K G V H E D T H K V H L L C L L A N G F Y R N N I C S Q P D L H A I G L S I I P A R F T R V L P R D V D T Y Y L S N L V K W F I G T F T V N A E L S A S E Q D N L Q T T L E R R F A I Y S A R D D E E L V H I F L L I L R A L Q L L T R L V L S L Q P I P L K S A T A K G K K P S K E R L T A D P G G S S E T S S Q V L E N H T K P K T S K G T K Q E E T F A K G T C R P S A K G K R N K G G R K K R S K P S S S E E D E G P G D K Q E K A T Q R R P H G R E R R V A S R V S Y K E E S G S D E A G S G S D F E L S S G E A S D P S D E D S E P G P P K Q R K A P A P Q R T K A G S K S A S R T H R G S H R K D P S L P V A S S S S S S S K R G K K M C S D G E K A E K R S I A G I D Q W L E V F C E Q E E K W V C V D C V H G V V G Q P L T C Y K Y A T K P M T Y V V G I D S D G W V R D V T Q R Y D P V W M T V T R K C R V D A E W W A E T L R P Y Q S P F M D R E K K E D L E F Q A K H M D Q P L P T A I G L Y K N H P L Y A L K R H L L K Y E A I Y P E T A A I L G Y C R G E A V Y S R D C V H T L H S R D T W L K K A R V V R L G E V P Y K M V K G F S N R A R K A R L A E P Q L R E E N D L G L F G Y W Q T E E Y Q P P V A V D G K V P R N E F G N V Y L F L P S M M P I G C V Q L N L P N L H R V A R K L D I D C V Q A I T G F D F H G G Y S H P V T D G Y I V C E E F K D V L L T A W E N E Q A V I E R K E K E K K E K R A L G N W K L L A K G L L I R E R L K R R Y G P K S E A A A P H T D A G G G L S S D E E E G T S S Q A E A A R I L A A S W P Q N R E D E E K Q K L K G G P K K T K R E K K A A A S H L F P F E K L

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Macromolecule #9: UV excision repair protein RAD23 homolog B

MacromoleculeName: UV excision repair protein RAD23 homolog B / type: protein_or_peptide / ID: 9 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: M Q V T L K T L Q Q Q T F K I D I D P E E T V K A L K E K I E S E K G K D A F P V A G Q K L I Y A G K I L N D D T A L K E Y K I D E K N F V V V M V T K P K A V S T P A P A T ...String:
M Q V T L K T L Q Q Q T F K I D I D P E E T V K A L K E K I E S E K G K D A F P V A G Q K L I Y A G K I L N D D T A L K E Y K I D E K N F V V V M V T K P K A V S T P A P A T T Q Q S A P A S T T A V T S S T T T T V A Q A P T P V P A L A P T S T P A S I T P A S A T A S S E P A P A S A A K Q E K P A E K P A E T P V A T S P T A T D S T S G D S S R S N L F E D A T S A L V T G Q S Y E N M V T E I M S M G Y E R E Q V I A A L R A S F N N P D R A V E Y L L M G I P G D R E S Q A V V D P P Q A A S T G A P Q S S A V A A A A A T T T A T T T T T S S G G H P L E F L R N Q P Q F Q Q M R Q I I Q Q N P S L L P A L L Q Q I G R E N P Q L L Q Q I S Q H Q E H F I Q M L N E P V Q E A G G Q G G G G G G G S G G I A E A G S G H M N Y I Q V T P Q E K E A I E R L K A L G F P E G L V I Q A Y F A C E K N E N L A A N F L L Q Q N F D E D L E H H H H H H

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Macromolecule #10: Centrin-2

MacromoleculeName: Centrin-2 / type: protein_or_peptide / ID: 10 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString:
MASNFKKANM ASSSQRKRMS PKPELTEEQK QEIREAFDLF DADGTGTIDV KELKVAMRAL GFEPKKEEI KKMISEIDKE GTGKMNFGDF LTVMTQKMSE KDTKEEILKA FKLFDDDETG K ISFKNLKR VAKELGENLT DEELQEMIDE ADRDGDGEVS EQEFLRIMKK TSLY

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Macromolecule #11: DNA1

MacromoleculeName: DNA1 / type: dna / ID: 11 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
SequenceString:
AGGTAGTCAC AGCTGATTGC GCTGAGGAT(Cy5) TAGACGTGCG ATATCTATGC CA

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Macromolecule #12: DNA2

MacromoleculeName: DNA2 / type: dna / ID: 12 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
SequenceString:
TGGCATAGAT ATCGCACGTC TATTATCCTC AGCGCAATCA GCTGTGACTA CCT

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.4 mg/mL
BufferpH: 7.9
Component:
ConcentrationNameFormula
25.0 mM4-(2-Hydroxyethyl)-1-piperazine ethanesulfonic acid
100.0 mMpotassium chlorideKCl
0.5 %Glycerol
2.0 mMMagnesium chlorideMgCl2
2.0 mM(Tris(2-carboxyethyl)phospine)
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 6243 / Average exposure time: 2.5 sec. / Average electron dose: 54.1 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 2.0 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1645921
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6nmi

Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 89824
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT / Target criteria: correlation coefficient

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