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データを開く
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基本情報
| 登録情報 | ![]() | |||||||||||||||||||||
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| タイトル | Composite cryo-EM density map of the 48-nm repeat of the human respiratory doublet microtubule | |||||||||||||||||||||
マップデータ | phenix auto-sharpened map | |||||||||||||||||||||
試料 |
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キーワード | cilia / microtubule / sperm / cell motility / STRUCTURAL PROTEIN | |||||||||||||||||||||
| 機能・相同性 | 機能・相同性情報axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath ...axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / protein polyglutamylation / regulation of calcineurin-NFAT signaling cascade / sperm axoneme assembly / regulation of microtubule nucleation / positive regulation of feeding behavior / inner dynein arm assembly / cilium-dependent cell motility / cerebrospinal fluid circulation / regulation of cilium beat frequency involved in ciliary motility / epithelial cilium movement involved in extracellular fluid movement / 9+2 motile cilium / cilium movement involved in cell motility / intraciliary transport / regulation of store-operated calcium entry / acrosomal membrane / axoneme assembly / cilium movement / left/right axis specification / microtubule sliding / calcium ion sensor activity / Post-chaperonin tubulin folding pathway / axonemal microtubule / Cilium Assembly / cytoskeleton-dependent intracellular transport / Carboxyterminal post-translational modifications of tubulin / organelle transport along microtubule / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / cilium organization / gamma-tubulin ring complex / forebrain morphogenesis / manchette / Sealing of the nuclear envelope (NE) by ESCRT-III / Intraflagellar transport / cerebellar cortex morphogenesis / positive regulation of cilium assembly / glial cell differentiation / neuron projection arborization / dentate gyrus development / Formation of tubulin folding intermediates by CCT/TriC / flagellated sperm motility / 3'-5'-DNA exonuclease activity / Gap junction assembly / UTP biosynthetic process / CTP biosynthetic process / Kinesins / Prefoldin mediated transfer of substrate to CCT/TriC / motile cilium / determination of left/right symmetry / intermediate filament / DNA catabolic process / pyramidal neuron differentiation / Assembly and cell surface presentation of NMDA receptors / response to L-glutamate / COPI-independent Golgi-to-ER retrograde traffic / centrosome cycle / positive regulation of cell motility / GTP biosynthetic process / COPI-dependent Golgi-to-ER retrograde traffic / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エンドデオキシリボヌクレアーゼ / natural killer cell mediated cytotoxicity / smoothened signaling pathway / perinuclear theca / AMP binding / ciliary base / regulation of neuron projection development / receptor clustering / regulation of synapse organization / startle response / cerebral cortex cell migration / ciliary transition zone / motor behavior / Recycling pathway of L1 / microtubule polymerization / mitotic cytokinesis / microtubule organizing center / cellular response to UV-C / locomotory exploration behavior / response to tumor necrosis factor / regulation of cell division / cilium assembly / cellular response to unfolded protein / MHC class I protein binding / spermatid development / negative regulation of protein binding / axoneme / single fertilization / glial cell projection / response to mechanical stimulus / sperm flagellum / alpha-tubulin binding 類似検索 - 分子機能 | |||||||||||||||||||||
| 生物種 | Homo sapiens (ヒト) | |||||||||||||||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | |||||||||||||||||||||
データ登録者 | Gui M / Croft JT / Zabeo D / Acharya V / Kollman JM / Burgoyne T / Hoog JL / Brown A | |||||||||||||||||||||
| 資金援助 | 米国, スウェーデン, 6件
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引用 | ジャーナル: Proc Natl Acad Sci U S A / 年: 2022タイトル: SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules. 著者: Miao Gui / Jacob T Croft / Davide Zabeo / Vajradhar Acharya / Justin M Kollman / Thomas Burgoyne / Johanna L Höög / Alan Brown / ![]() 要旨: The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins ...The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule: the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients. | |||||||||||||||||||||
| 履歴 |
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構造の表示
| 添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
-関連構造データ
| 関連構造データ | ![]() 7ungMC ![]() 7un1C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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| 「今月の分子」の関連する項目 |
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マップ
| ファイル | ダウンロード / ファイル: emd_26624.map.gz / 形式: CCP4 / 大きさ: 512 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| 注釈 | phenix auto-sharpened map | ||||||||||||||||||||||||||||||||||||
| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 1.37 Å | ||||||||||||||||||||||||||||||||||||
| 密度 |
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| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
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-添付データ
+マスク #1
+追加マップ: local masked map
+追加マップ: stitched unsharpened map
+追加マップ: consensus refined map
+追加マップ: local masked map
+追加マップ: local masked map
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+追加マップ: local masked map
+追加マップ: local masked map
+追加マップ: deepEMhancer sharpened map
+追加マップ: local masked map
+追加マップ: local masked map
+追加マップ: local masked map
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+追加マップ: local masked map
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+追加マップ: local masked map
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+追加マップ: local masked map
+追加マップ: local masked map
+追加マップ: local masked map
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+追加マップ: local masked map
+ハーフマップ: #2
+ハーフマップ: #1
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試料の構成要素
+全体 : Doublet microtubule and associated proteins
+超分子 #1: Doublet microtubule and associated proteins
+分子 #1: Protein CFAP95
+分子 #2: EF-hand domain-containing family member B
+分子 #3: Cilia- and flagella-associated protein 53
+分子 #4: Nucleoside diphosphate kinase 7
+分子 #5: Protein CFAP107
+分子 #6: Protein CFAP141
+分子 #7: Tektin-1
+分子 #8: Tubulin alpha-1A chain
+分子 #9: Tubulin beta-4B chain
+分子 #10: Meiosis-specific nuclear structural protein 1
+分子 #11: Tektin-2
+分子 #12: Tektin-3
+分子 #13: Sperm-associated antigen 8
+分子 #14: Tektin-4
+分子 #15: Cilia- and flagella-associated protein 161
+分子 #16: Sperm acrosome-associated protein 9
+分子 #17: Uncharacterized protein C15orf65
+分子 #18: Protein FAM166B
+分子 #19: UPF0686 protein C11orf1
+分子 #20: Isoform 2 of Cilia- and flagella-associated protein 77
+分子 #21: Protein FAM183A
+分子 #22: Uncharacterized protein C5orf49
+分子 #23: Protein FAM166C
+分子 #24: RIB43A-like with coiled-coils protein 2
+分子 #25: EF-hand domain-containing protein 1
+分子 #26: EF-hand domain-containing family member C2
+分子 #27: Cilia- and flagella-associated protein 20
+分子 #28: Parkin coregulated gene protein
+分子 #29: Cilia- and flagella-associated protein 45
+分子 #30: Cilia- and flagella-associated protein 52
+分子 #31: Enkurin
+分子 #32: Protein Flattop
+分子 #33: Protein CFAP210
+分子 #34: Protein CFAP276
+分子 #35: UPF0691 protein C9orf116
+分子 #36: GUANOSINE-5'-TRIPHOSPHATE
+分子 #37: MAGNESIUM ION
+分子 #38: GUANOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
| 試料の集合状態 | filament |
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試料調製
| 緩衝液 | pH: 7.3 |
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| グリッド | モデル: Quantifoil R2/1 / 前処理 - タイプ: GLOW DISCHARGE |
| 凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % |
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電子顕微鏡法
| 顕微鏡 | FEI TITAN KRIOS |
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| 撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 60.0 e/Å2 |
| 電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.8 µm / 倍率(公称値): 64000 |
| 試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
Homo sapiens (ヒト)
データ登録者
米国,
スウェーデン, 6件
引用

































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解析
FIELD EMISSION GUN


