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-Structure paper
Title | SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 119, Issue 41, Page e2207605119, Year 2022 |
Publish date | Oct 11, 2022 |
Authors | Miao Gui / Jacob T Croft / Davide Zabeo / Vajradhar Acharya / Justin M Kollman / Thomas Burgoyne / Johanna L Höög / Alan Brown / |
PubMed Abstract | The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins ...The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule: the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients. |
External links | Proc Natl Acad Sci U S A / PubMed:36191189 / PubMed Central |
Methods | EM (tomography) / EM (subtomogram averaging) / EM (single particle) |
Resolution | 3.6 - 20.0 Å |
Structure data | EMDB-14723: Tomogram of bovine sperm singlet microtubules EMDB-14730: Tomogram of chicken sperm distal microtubules EMDB-14732: Tomogram of frog sperm singlet distal microtubules EMDB-15451: Sub-tomogram average of TAILS in bovine sperm tail tip singlet microtubules. EMDB-26611: Composite cryo-EM density map of the 8-nm repeat of the human sperm tip singlet microtubule EMDB-26624: Composite cryo-EM density map of the 48-nm repeat of the human respiratory doublet microtubule |
Chemicals | ChemComp-GDP: ChemComp-GTP: ChemComp-MG: |
Source |
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Keywords | STRUCTURAL PROTEIN / cilia / microtubule / sperm / cell motility |