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Yorodumi- EMDB-15451: Sub-tomogram average of TAILS in bovine sperm tail tip singlet mi... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-15451 | |||||||||
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Title | Sub-tomogram average of TAILS in bovine sperm tail tip singlet microtubules. | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Microtubules Flagella Bovine Sperm / UNKNOWN FUNCTION | |||||||||
Biological species | Bos taurus (cattle) | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 20.0 Å | |||||||||
Authors | Zabeo D / Hoog JL | |||||||||
Funding support | Sweden, 1 items
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Citation | Journal: Proc Natl Acad Sci U S A / Year: 2022 Title: SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules. Authors: Miao Gui / Jacob T Croft / Davide Zabeo / Vajradhar Acharya / Justin M Kollman / Thomas Burgoyne / Johanna L Höög / Alan Brown / Abstract: The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins ...The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule: the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_15451.map.gz | 2.9 MB | EMDB map data format | |
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Header (meta data) | emd-15451-v30.xml emd-15451.xml | 12.9 KB 12.9 KB | Display Display | EMDB header |
Images | emd_15451.png | 55.7 KB | ||
Filedesc metadata | emd-15451.cif.gz | 3.8 KB | ||
Others | emd_15451_half_map_1.map.gz emd_15451_half_map_2.map.gz | 2.9 MB 2.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-15451 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15451 | HTTPS FTP |
-Validation report
Summary document | emd_15451_validation.pdf.gz | 807.4 KB | Display | EMDB validaton report |
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Full document | emd_15451_full_validation.pdf.gz | 807 KB | Display | |
Data in XML | emd_15451_validation.xml.gz | 7.9 KB | Display | |
Data in CIF | emd_15451_validation.cif.gz | 9.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15451 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15451 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_15451.map.gz / Format: CCP4 / Size: 3.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.37 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_15451_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_15451_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : SPACA9 inner microtubule decoration.
Entire | Name: SPACA9 inner microtubule decoration. |
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Components |
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-Supramolecule #1: SPACA9 inner microtubule decoration.
Supramolecule | Name: SPACA9 inner microtubule decoration. / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Bos taurus (cattle) / Tissue: Sperm / Organelle: Flagellum |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | subtomogram averaging |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.64 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 4.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 20.0 Å / Resolution method: OTHER / Software - Name: PEET / Number subtomograms used: 14549 |
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Extraction | Number tomograms: 10 / Number images used: 14549 / Software - Name: PEET |
Final angle assignment | Type: NOT APPLICABLE / Software - Name: PEET |