|Entry||Database: EMDB / ID: 2609|
|Title||KIF14 Motor Domain Microtubule complex|
|Keywords||Kinesin / Micotubule / Tubulin / KIF14 / Kinesin-3|
|Sample||Kinesin KIF14 motor domain microtubule complex|
|Source||Mus musculus / mammal / ハツカネズミ, はつかねずみ / |
Bos taurus / mammal / ウシ /
|Map data||kinesin KIF14 mtor domain micerotubule complex|
|Method||helical reconstruction, at 15.5 Å resolution|
|Authors||Arora K / Talje L / Asenjo AB / Andersen P / Atchia K / Joshi M / Sosa H / Allingham JS / Kwok BH|
|Citation||J. Mol. Biol., 2014, 426, 2997-3015|
|Date||Deposition: Mar 20, 2014 / Header (metadata) release: Apr 2, 2014 / Map release: Nov 19, 2014 / Last update: Nov 19, 2014|
Downloads & links
|File||emd_2609.map.gz (map file in CCP4 format, 941 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 2 Å|
CCP4 map header:
-Entire Kinesin KIF14 motor domain microtubule complex
|Entire||Name: Kinesin KIF14 motor domain microtubule complex / Number of components: 2|
-Component #1: protein, Kinesin KIF14 motor domain
|Protein||Name: Kinesin KIF14 motor domain / Recombinant expression: Yes|
|Source||Species: Mus musculus / mammal / ハツカネズミ, はつかねずみ /|
|Source (engineered)||Expression System: Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /|
-Component #2: protein, Tubulin
|Protein||Name: Tubulin / Recombinant expression: No|
|Source||Species: Bos taurus / mammal / ウシ /|
|Specimen state||helical array|
|Helical parameters||Axial symmetry: C1 (asymmetric) / Hand: RIGHT HANDED / Delta z: 5.40237 Å / Delta phi: 168.08788 deg.|
|Vitrification||Instrument: FEI VITROBOT MARK I / Cryogen name: ETHANE / Humidity: 100 %|
-Electron microscopy imaging
|Imaging||Microscope: FEI TECNAI 20 / Date: Nov 25, 2013|
|Electron gun||Electron source: LAB6 / Accelerating voltage: 120 kV / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD / Defocus: 1000 - 2600 nm|
|Specimen Holder||Model: GATAN LIQUID NITROGEN|
|Camera||Detector: TVIPS TEMCAM-F415 (4k x 4k)|
|Image acquisition||Number of digital images: 30|
|Processing||Method: helical reconstruction|
Details: Initial reference map obtained by Fourier-Bessel reconstruction as implemented in SUPRIM & PHOELIX Single particle alignment, 3D reconstruction and refinement done using IHRSR, SPIDER and custom routines.
|3D reconstruction||Software: SUPRIM, PHOELIX, IHRSR, SPIDER, CTFFIND3, CUSTOM, (emglue.py)|
CTF correction: Each Particle / Resolution: 15.5 Å / Resolution method: FSC 0.143, gold-standard
-Atomic model buiding
|Modeling #1||Software: Chimera / Refinement protocol: rigid body / Refinement space: REAL|
Details: The 2 domains (tubulin heterodimer and KIF14 motor domain) were separately fitted. 1 nm resolution density maps of the atomic models were fitted within the cryo-em map using the global fit option of the fitmap command of the UCSF-CHIMERA program.
Input PDB model: 4OZQ
|Modeling #2||Refinement protocol: rigid body / Refinement space: REAL|
Input PDB model: 1JFF
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