Translation Initiation / Protein synthesis / bS21 / RIBOSOME
機能・相同性
機能・相同性情報
ribosomal large subunit assembly / large ribosomal subunit / small ribosomal subunit / transferase activity / 5S rRNA binding / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / mRNA binding 類似検索 - 分子機能
Protein of unknown function DUF4295 / Domain of unknown function (DUF4295) / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 ...Protein of unknown function DUF4295 / Domain of unknown function (DUF4295) / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein S14/S29 / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S19, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein L20 signature. / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / L28p-like / Ribosomal protein L20 / Ribosomal protein S15, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily 類似検索 - ドメイン・相同性
30S ribosomal protein S12 / 50S ribosomal protein L32 / 50S ribosomal protein L22 / 50S ribosomal protein L24 / 50S ribosomal protein L16 / 30S ribosomal protein S5 / 30S ribosomal protein S4 / 50S ribosomal protein L2 / 30S ribosomal protein S14 / 50S ribosomal protein L34 ...30S ribosomal protein S12 / 50S ribosomal protein L32 / 50S ribosomal protein L22 / 50S ribosomal protein L24 / 50S ribosomal protein L16 / 30S ribosomal protein S5 / 30S ribosomal protein S4 / 50S ribosomal protein L2 / 30S ribosomal protein S14 / 50S ribosomal protein L34 / 50S ribosomal protein L20 / 50S ribosomal protein L28 / 30S ribosomal protein S20 / 50S ribosomal protein L33 / 30S ribosomal protein S18 / 30S ribosomal protein S6 / 50S ribosomal protein L23 / 50S ribosomal protein L35 / 30S ribosomal protein S11 / 30S ribosomal protein S3 / 30S ribosomal protein S21 / 30S ribosomal protein S10 / 50S ribosomal protein L13 / 50S ribosomal protein L21 / 50S ribosomal protein L25 / Uncharacterized protein / 50S ribosomal protein L31 / 50S ribosomal protein L17 / 50S ribosomal protein L30 / 50S ribosomal protein L6 / 30S ribosomal protein S8 / 30S ribosomal protein S17 / 30S ribosomal protein S19 / 30S ribosomal protein S7 / 50S ribosomal protein L3 / 50S ribosomal protein L9 / 50S ribosomal protein L19 / 30S ribosomal protein S16 / 30S ribosomal protein S15 / 50S ribosomal protein L27 / 30S ribosomal protein S9 / 50S ribosomal protein L4 / 50S ribosomal protein L15 / 50S ribosomal protein L18 / 50S ribosomal protein L36 / Uncharacterized protein / 50S ribosomal protein L14 / 50S ribosomal protein L29 類似検索 - 構成要素
Natural Sciences and Engineering Research Council (NSERC, Canada)
RGPIN-2019-05799
カナダ
引用
ジャーナル: Nucleic Acids Res / 年: 2021 タイトル: Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome. 著者: Vikash Jha / Bappaditya Roy / Dushyant Jahagirdar / Zakkary A McNutt / Elan A Shatoff / Bethany L Boleratz / Dean E Watkins / Ralf Bundschuh / Kaustuv Basu / Joaquin Ortega / Kurt Fredrick / 要旨: Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the ...Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the canonical anti-SD (ASD) sequence. Here, we show that ribosomes purified from Flavobacterium johnsoniae, a representative of the Bacteroidetes, fail to recognize the SD sequence of mRNA in vitro. A cryo-electron microscopy structure of the complete 70S ribosome from F. johnsoniae at 2.8 Å resolution reveals that the ASD is sequestered by ribosomal proteins bS21, bS18 and bS6, explaining the basis of ASD inhibition. The structure also uncovers a novel ribosomal protein-bL38. Remarkably, in F. johnsoniae and many other Flavobacteriia, the gene encoding bS21 contains a strong SD, unlike virtually all other genes. A subset of Flavobacteriia have an alternative ASD, and in these organisms the fully complementary sequence lies upstream of the bS21 gene, indicative of natural covariation. In other Bacteroidetes classes, strong SDs are frequently found upstream of the genes for bS21 and/or bS18. We propose that these SDs are used as regulatory elements, enabling bS21 and bS18 to translationally control their own production.