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- EMDB-22345: 70S ribosome Flavobacterium johnsoniae -

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Basic information

Entry
Database: EMDB / ID: EMD-22345
Title70S ribosome Flavobacterium johnsoniae
Map data70S ribosome Flavobacterium johnsoniae
Sample
  • Complex: 70S ribosome Flavobacterium johnsoniae
    • Protein or peptide: x 50 types
    • RNA: x 2 types
  • RNA: x 1 types
  • Ligand: x 1 types
KeywordsTranslation Initiation / Protein synthesis / bS21 / RIBOSOME
Function / homology
Function and homology information


large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome ...large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / cytoplasm
Similarity search - Function
Protein of unknown function DUF4295 / Domain of unknown function (DUF4295) / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 ...Protein of unknown function DUF4295 / Domain of unknown function (DUF4295) / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L20 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L34, conserved site / Ribosomal L28 family / Ribosomal protein L34 signature. / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein L20 / Ribosomal protein S15, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein L19 superfamily
Similarity search - Domain/homology
Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein bL34 ...Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL25 / DUF4295 domain-containing protein / 50S ribosomal protein L31 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL18 / 50S ribosomal protein L36 / Uncharacterized protein / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL29
Similarity search - Component
Biological speciesFlavobacterium johnsoniae (bacteria) / Flavobacterium sp. Root186 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsOrtega J
Funding support Canada, 1 items
OrganizationGrant numberCountry
Natural Sciences and Engineering Research Council (NSERC, Canada)RGPIN-2019-05799 Canada
CitationJournal: Nucleic Acids Res / Year: 2021
Title: Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome.
Authors: Vikash Jha / Bappaditya Roy / Dushyant Jahagirdar / Zakkary A McNutt / Elan A Shatoff / Bethany L Boleratz / Dean E Watkins / Ralf Bundschuh / Kaustuv Basu / Joaquin Ortega / Kurt Fredrick /
Abstract: Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the ...Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the canonical anti-SD (ASD) sequence. Here, we show that ribosomes purified from Flavobacterium johnsoniae, a representative of the Bacteroidetes, fail to recognize the SD sequence of mRNA in vitro. A cryo-electron microscopy structure of the complete 70S ribosome from F. johnsoniae at 2.8 Å resolution reveals that the ASD is sequestered by ribosomal proteins bS21, bS18 and bS6, explaining the basis of ASD inhibition. The structure also uncovers a novel ribosomal protein-bL38. Remarkably, in F. johnsoniae and many other Flavobacteriia, the gene encoding bS21 contains a strong SD, unlike virtually all other genes. A subset of Flavobacteriia have an alternative ASD, and in these organisms the fully complementary sequence lies upstream of the bS21 gene, indicative of natural covariation. In other Bacteroidetes classes, strong SDs are frequently found upstream of the genes for bS21 and/or bS18. We propose that these SDs are used as regulatory elements, enabling bS21 and bS18 to translationally control their own production.
History
DepositionJul 23, 2020-
Header (metadata) releaseDec 2, 2020-
Map releaseDec 2, 2020-
UpdateMay 15, 2024-
Current statusMay 15, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 2.8
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 2.8
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7jil
  • Surface level: 2.8
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_22345.png

Downloads & links

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Map

FileDownload / File: emd_22345.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation70S ribosome Flavobacterium johnsoniae
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
1.07 Å/pix.
x 360 pix.
= 386.28 Å		1.07 Å/pix.
x 360 pix.
= 386.28 Å		1.07 Å/pix.
x 360 pix.
= 386.28 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.073 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 2.8 / Movie #1: 2.8
Minimum - Maximum-15.121297999999999 - 26.298506
Average (Standard dev.)-0.000000000000249 (±1.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 386.27997 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0731.0731.073
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z386.280386.280386.280
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ360360360
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-15.12126.299-0.000

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Supplemental data

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Sample components

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Entire : 70S ribosome Flavobacterium johnsoniae

EntireName: 70S ribosome Flavobacterium johnsoniae
Components
  • Complex: 70S ribosome Flavobacterium johnsoniae
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • RNA: RNA (2862-MER)
    • RNA: RNA (111-MER)
    • Protein or peptide: 50S ribosomal protein L38
    • Protein or peptide: 30S ribosomal protein S22
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S21
  • RNA: RNA (1519-MER)
  • Ligand: water

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Supramolecule #1: 70S ribosome Flavobacterium johnsoniae

SupramoleculeName: 70S ribosome Flavobacterium johnsoniae / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#24, #26-#53
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)

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Macromolecule #1: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 29.991619 KDa
SequenceString: MSVRKLKPIT PGQRFRVVNG YDAITTDKPE RSLISPIKNS GGRNSQGKMT MRYTGGGHKQ RYRIIDFKRT KDGIPATVKS IEYDPNRTA FIALLAYADG EKTYIIAQNG LKVGQKLVSG PESQPEIGNT LPLSRIPLGT VISCIELRPG QGAVIARSAG T FAQLMARD ...String:
MSVRKLKPIT PGQRFRVVNG YDAITTDKPE RSLISPIKNS GGRNSQGKMT MRYTGGGHKQ RYRIIDFKRT KDGIPATVKS IEYDPNRTA FIALLAYADG EKTYIIAQNG LKVGQKLVSG PESQPEIGNT LPLSRIPLGT VISCIELRPG QGAVIARSAG T FAQLMARD GKYATIKMPS GETRLILLTC SATIGEVSNS DHQLVVSGKA GRTRWLGRRP RTRPVAMNPV DHPMGGGEGR SS GGHPRSR NGLPAKGYRT RSKKNPSNKY IVERRKK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #2: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 21.975359 KDa
SequenceString: MSGLIGKKIG MTSIFDENGK NIPCTVIEAG PCVVTQVRTN EVDGYEALQL GFDDKNEKHS TKAALGHFKK AGTVAKKKVV EFQDFAAAQ ALGDLIDVSI FEEGEFVDVQ GVSKGKGFQG VVKRHGFGGV GQATHGQHQR LRAPGSVGAS SYPSRVFKGM R MAGRMGGD ...String:
MSGLIGKKIG MTSIFDENGK NIPCTVIEAG PCVVTQVRTN EVDGYEALQL GFDDKNEKHS TKAALGHFKK AGTVAKKKVV EFQDFAAAQ ALGDLIDVSI FEEGEFVDVQ GVSKGKGFQG VVKRHGFGGV GQATHGQHQR LRAPGSVGAS SYPSRVFKGM R MAGRMGGD NVKVQNLRVL KVVAEKNLLV VKGCIPGHKN SYVIIQK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #3: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 23.187557 KDa
SequenceString: MEVKVLDFNG KDTGRKVQLS DSVFAIEPNN HAVYLDVKQY LANQRQGTHK AKERAEVTGS TRKIKKQKGT GTARAGSVKN PLFKGGGTV FGPRPRSYSF KLNKNLKRLA RKSAFSIKAK ESNIIVLEDF NFEAPNTKNF INVLKALGLE NKKSLFVLGE S NKNVYLSS ...String:
MEVKVLDFNG KDTGRKVQLS DSVFAIEPNN HAVYLDVKQY LANQRQGTHK AKERAEVTGS TRKIKKQKGT GTARAGSVKN PLFKGGGTV FGPRPRSYSF KLNKNLKRLA RKSAFSIKAK ESNIIVLEDF NFEAPNTKNF INVLKALGLE NKKSLFVLGE S NKNVYLSS RNLKASNVVT SSELSTYAIL NTNNLVLLEG SLELIEENLS K

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #4: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 20.79932 KDa
SequenceString:
MAYTPRLKEE YKSRVISALK EEFGYTNVMQ VPKLEKIVLS RGVGAAVSDK KLIDYAVDEL TKITGQKAVI TKARKSVAGF KIRQGYPIG CKVTLRGERM WEFFERLITI AVPRIRDFRG LSAKSFDGRG NYSMGVREQI IFPEIDYDKV DRVRGMDITF V TTAKTDKE AKSLLAELGL PFKKN

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Macromolecule #5: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 19.420445 KDa
SequenceString:
MSRIGKSPIV IPAGVTVEVK DGIITVKGKK GQLVQEFSDV NVTVEGDQVL VERSSDHKDH RAKHGLFRSL ISNMVVGVSE GFTKELELV GVGYRAANQG NKLDLALGYS HNIVLEIAPE VSLETISEAG ANPIVKLTSF DKQLLGQVAA KIRGFRKPEP Y KGKGVKFV GEVLRRKAGK SA

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #6: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.968622 KDa
SequenceString:
MEIILKQDVQ NLGFKDDVVS VKPGYGRNFL IPQGFATLAT PSAKKVLAEN LKQRAHKEAK IVADAKALAE TLKALEIKLT AKAGGEKLF GSITNIDIAE ALEKSGNAID RKFITSGVVK RIGKYNATVR LHRDVIVELP YEIVAEK

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #7: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 16.578273 KDa
SequenceString:
MDALSYKTIS ASKATVTKEW IVVDAEGHNL GRLASKVAMI LRGKYKPSYT PHVDCGDNVI VINSEKINLT GTKLNDKIYM RHTGYPGGQ RTLTAKVLQS KNPALLVEKA VKGMLPKNKL GAELFRNLNV VVGSEHTHGA QKPRTVNLND LK

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #8: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.339537 KDa
SequenceString:
MVQQESRLKV ADNTGAKEVL TIRVLGGTKR RYASVGDKIV VSIKDATPNG NVKKGAVSTA VVVRTKKEVR RADGSYIRFD DNACVLLNA AGEMRGTRVF GPVARELREK QFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #9: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.994265 KDa
SequenceString:
MNLSNLQPAE GSTHNQNKRV GRGEGSGKGG TAARGHKGAK SRSGYSKKIG FEGGQMPLQR RVPKFGFKNI NRKEYEGVNL DTLQLLVDN GVITDSVSMT DFVANRLASK NEIVKILGRG ELKAKLKVTA HKFTATAKAA IEAAGGEAVT I

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #10: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 16.075122 KDa
SequenceString:
MLQPKRTKYR KVQKGRMKGN SQRGHELSNG MFGIKSVHED GMFLTSRQIE AARIAATRYM KREGQLWIKI FPDKPITKKP LEVRMGKGK GAVEYWWAVV KPGRIMFEVG GVPLSVAKEA LRLAAQKLPV KTKFVVARDF EA

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #11: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 18.092869 KDa
SequenceString:
MRHGKKINHL SRQTGHRKAM LANMACSLIE HKRINTTVAK AKALKQFVEP LITKSKEDTT HNRRIVFAYL RSKYAVTDLF RDVAAKVGD RPGGYTRIIK VGNRLGDNAD MAMIELVDFN ELYNGGKKEV KKAKSRRGGK AKKAEGTAPE APAAESESTT E ASE

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #12: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 12.66072 KDa
SequenceString:
MSLTKSERRQ RIKFRIRKTI SGSAAKPRLS VFRSNKEIYA QLIDDVNGVT ILAASSREKE IAKGTNVEIA AAVGKLVAEK ALKAGIDTI TFDRGGYLYH GRIKSLAEGA RAAGLKF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #13: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.316622 KDa
SequenceString:
MADLLKFVQN EFVAKKDFPD FGAGDTITVF YEIKEGEKTR TQFFKGVVIQ RRGSGTTETF TIRKMSGAIG VERIFPVNLP ALQKIEINK KGAVRRARIF YFRELTGKKA KIKDKLR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #14: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.244699 KDa
SequenceString:
MPRSVNSVAK RARRKKIMKQ AKGFFGRRKN VWTVAKNAVE KAMSYAYRDR KQNKRNFRSL WIQRINAGAR LEGMSYSQFM GKVKANGIE LNRKVLADLA MNHPEAFKAI LNKVK

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #15: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.90152 KDa
SequenceString:
MYAIVEIAGQ QFKVSKDLKV YVHRLANEEG SKVSFDKVLL LDDNGNVTLG APAIEGASVE AKVLQHLKGD KVIVFKKKRR KGYKKRNGH RQYLTQIVIE GITAAGGTKK AAAKKAVVAE EAAATEEVEA APKAKKAAPK AKKEATKE

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #16: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.475993 KDa
SequenceString:
MGVRKRETAD ARKEANKSIA FAKLNNCPTS PRKMRLVADL VRGQKVERAL NILRFSSKEA SRKLEKLLLS AINNWEQKNS EGNLEEAGL FVKEIRVDGG MMLKRLRPAP QGRAHRIRKR SNHVTIVLGA INNTQSNS

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #17: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.690438 KDa
SequenceString:
MSIIIRPIVT EKVTKESEVL NRFGFVVDKK ANKVQIKKAV EAAYGVTIVS VNTMNVRPDR STKYTKSGLI SGKTNAIKKA IVQVQEGET IDFYNNI

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #18: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 11.439517 KDa
SequenceString:
MIKLKIKSGD IVRVIAGDHK GAEGKVLRVY REKNKAIVEG VNLVSKHTKP SAKNPQGGIV KKEASIQISN IALIDPKTKE TTRVGIRVE GDKKVRFSKK SNQVL

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #19: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 21.89165 KDa
SequenceString: MKSITIKGSE RESVGKVSTK ALRNAGAVPC VLYGGNQAVH FSADAAAFKN LVYTPNAHTV VIELGAGKSF NAILQDIQVH PVTDKILHI DFFQLFDDKE ITMEVPVKIV GTSKGVLAGG VLRLNQRKLK VKALPANIPE FVEADITPLE MGNKLYVTKV G KPEYKIMH ...String:
MKSITIKGSE RESVGKVSTK ALRNAGAVPC VLYGGNQAVH FSADAAAFKN LVYTPNAHTV VIELGAGKSF NAILQDIQVH PVTDKILHI DFFQLFDDKE ITMEVPVKIV GTSKGVLAGG VLRLNQRKLK VKALPANIPE FVEADITPLE MGNKLYVTKV G KPEYKIMH PDNTVVAQVR ISRAAMKAAQ EAAKAAKAPA KGKKK

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #20: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 9.422824 KDa
SequenceString:
MAHKKGVGSS KNGRESESKR LGVKIYGGQA AIAGNIIVRQ RGSKHNPGEN VYISKDHTLH ARVAGVVKFQ KKRDNKSYVS IIPFEA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #21: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 8.942626 KDa
SequenceString:
MSRVCDLTGK RAMVGNNVSH AMNKTKRKFS VNLVKKRFYL PEEDRWITLR VQRSTIKTIN KNGITAVLKK AQSAGFIK

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #22: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.189451 KDa
SequenceString:
MKQSEIKDLS AAELQEKLSQ TKKVYADLKM AHAISPIANP LRIRSVRRTV ARLATELTKR ELQ

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #23: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 6.643993 KDa
SequenceString:
MAKLLVKQVR SKINCPLSQK RGLEALGLRK LGQVVEHESN PAILGMINKV KHLVSVEEAK

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #27: 50S ribosomal protein L38

MacromoleculeName: 50S ribosomal protein L38 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 5.569749 KDa
SequenceString:
MAKKTVASLQ TSSKRLSKAI KMVKSPKTGA YIFVESIMAP ELVDEFLKKK

UniProtKB: DUF4295 domain-containing protein

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Macromolecule #28: 30S ribosomal protein S22

MacromoleculeName: 30S ribosomal protein S22 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 3.696608 KDa
SequenceString:
MPSGKKRKRH KVATHKRKKR ARANRHKKKK

UniProtKB: Uncharacterized protein

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Macromolecule #29: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 9.646128 KDa
SequenceString:
MKKGIHPENY RLVAFKDMSN DDVFITKSTA DTKETIEVDG VEYPVVKMEI SRTSHPFYTG KSKLIDTAGR IDKFKTKYAK HAKK

UniProtKB: 50S ribosomal protein L31

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Macromolecule #30: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.518691 KDa
SequenceString:
MAHPKRKTSK TRRDKRRTHY KATVAQIATC PITGEAHLYH RAYWHEGKMY YRGQVVIDKS EAVA

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #31: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 6.935232 KDa
SequenceString:
MAKKGNRIQV ILECTEHKTS GVPGTSRYIT TKNKKNTPDR LEIKKFNPIL KRVTVHKEIK

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #32: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 6.324544 KDa
SequenceString:
MSKRTFQPSK RKRRNKHGFM DRMASANGRK VLARRRAKGR HKLTVSSEPR HKK

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #33: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.522242 KDa
SequenceString:
MPKMKTKSSA KKRFKVTGSG KIKRKHAFKS HILTKKSKKR KLALTHSALV HKTDEKSIKQ QLRII

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #34: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 4.557577 KDa
SequenceString:
MKVRASVKKR SAECIIVRRK GRLYVINKKN PRFKQRQG

UniProtKB: 50S ribosomal protein L36

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Macromolecule #35: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 27.886365 KDa
SequenceString: MGQKTNPIGN RLGIIRGWDS NWYGGNDYGD KLAEDHKIRK YIHARLSKAS VSKVIIERTL KLVTVTITTA RPGIIIGKGG QEVDKLKEE LKKVTDKEVQ INIFEIKRPE LDAYLVATSI ARQIESRISY RRAIKMAIAA SMRMNAEGIK VLISGRLNGA E MARSEGFK ...String:
MGQKTNPIGN RLGIIRGWDS NWYGGNDYGD KLAEDHKIRK YIHARLSKAS VSKVIIERTL KLVTVTITTA RPGIIIGKGG QEVDKLKEE LKKVTDKEVQ INIFEIKRPE LDAYLVATSI ARQIESRISY RRAIKMAIAA SMRMNAEGIK VLISGRLNGA E MARSEGFK EGRIPLSTFR ADIDYALAEA HTTYGRMGIK VWIMKGEVYG KRDLSPLAGM DKKQSGTGGG KGGDAPRGKS NF NKGGKPD ARKRK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #36: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 22.950443 KDa
SequenceString: MARYTGPKTK IARKFGEAIF GDDKSFEKRN YPPGQHGMAK KRGKKSEYAV QLMEKQKAKY SYGILEKQFR NLFEKASATK GVTGEVLLQ LCEARLDNVV FRMGIAPSRR GARQIVSHRH ITVNGEVVNI PSYHLKPGDK VAVREKSKSL EAIERSLSNS S HVYEWITW ...String:
MARYTGPKTK IARKFGEAIF GDDKSFEKRN YPPGQHGMAK KRGKKSEYAV QLMEKQKAKY SYGILEKQFR NLFEKASATK GVTGEVLLQ LCEARLDNVV FRMGIAPSRR GARQIVSHRH ITVNGEVVNI PSYHLKPGDK VAVREKSKSL EAIERSLSNS S HVYEWITW NNDLKEGTFV SVPARLQIPE NIKEQLIVEL YNK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #37: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 18.20602 KDa
SequenceString:
MSKYKNVELV KPSGLELKDR LVSVNRVTKV TKGGRAFGFS AIVVVGDENG VVGHGLGKSK DVSEAIAKAV EDAKKNLVRI PLNGQSVPH EQKGKFGGAR VFLIPASHGT GVIAGGAVRS VLESVGIHDV LSKSQGSSNP HNVVKATFDA LLQMRSAHTV A KQRGVSLE KVFKG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #38: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.376448 KDa
SequenceString:
MNHYETVFIL NPVLSEVQVK ETVTKFEEFL TSRGAEMVSK EDWGLKKMAY EIQNKKSGFY HLFEFKVAGE VLIAFETEFR RDERVMRFL TVSLDKHAIS WAERRRAKLK STKA

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #39: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 18.493707 KDa
SequenceString:
MRKRAAKKRP LLPDPRFNDQ LVTRFVNNLM WDGKKSTAFK VFYDAIDIIE TKKQNDEKTS LEIWKDALTN VMPHVEVRSR RVGGATFQI PMQIRPDRKI SMAMKWLILY ARRRNEKSMA QRLASECLAA AKEEGAAVKK RMDTHKMAEA NKAFSHFRF

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #40: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 14.652163 KDa
SequenceString:
MYTDPIADYL TRVRNAVAAN HKVVEIPASN LKKEITKILF DQGYILSYKF EQNTVQGSIK IALKYDKDTK EPVIKDIQRI SKPGLRKYA GAAKLPRILN GLGIAIVSTS KGLMTGKQAK QLNVGGEVIC YVY

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #41: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 14.464888 KDa
SequenceString:
MGVIHKIGRR KTAVARVYVS EGTGNITVNK KEFATYFPTA TLQYKVLQPL SMTENVNNFD VKVNVYGGGT TGQAEAVRMA LARVMCEVN AENRGILKPE GLLTRDPRMV ERKKFGQKKA RKRFQFSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #42: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 11.44664 KDa
SequenceString:
MSQKIRIKLK SYDHMLVDKS AEKIVKTVKT TGAVVTGPIP LPTHKKLFTV LRSPHVNKKA REQFEVMSYK RLIDIYSSSS KTIDALMKL ELPSGVEVEI KV

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #43: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.700957 KDa
SequenceString:
MAKATAKKRK VIVESTGEAH ISSTFNNIII SLTNKKGEVI AWSSAGKMGF RGSKKNTPYA AQMAAEDCSK VALEAGLKKV KVYVKGPGN GRESAIRSIH NGGIEVTEII DVTPMPHNGC RPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #44: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium sp. Root186 (bacteria)
Molecular weightTheoretical: 14.121425 KDa
SequenceString:
MPTIQQLVRT GRTQITKKSK SVALDSCPQR RGVCTRVYTT TPKKPNSAMR KVARVRLTNG NEVNAYIPGE GHNLQEHSIV LVRGGRVKD LPGVRYHIVR GALDTSGVAG RTQRRSKYGA KRPKEAKK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #45: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.734152 KDa
SequenceString:
MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKTVANK KKATK

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Macromolecule #46: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.211011 KDa
SequenceString:
MAKESMKARE VKREKTVAKY AEKRKALLEA GDYEGLQRLP KNASPVRLHN RCKLTGRPRG YIRQFGISRV TFREMANNGL IPGVKKASW

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #47: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.565339 KDa
SequenceString:
MYLTKEKKEE IFAQHGDAKN TGKAEGQIAL FTYRISHLTE HLKKNRHDYN TERSLVLLVG KRRALLDYLK RKDINRYREI IKVLNIRK

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #48: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 20.106588 KDa
SequenceString:
MSVKIRLQRH GKKGKPFYWV VAADARSKRD GKYLEKIGTY NPNTNPATVE LNLDSIVKWL HQGAQPTDTA RAILSYKGAL LKHHLDGGV RKGALTQEQA DAKLTAWLEA KAGKVDAKKD GLSKAQADAK AKALKAEKEV NDKRIAAAAK AEADAIAAAS A TEEAATEE VAEAAEEAPA AEENNETTEA

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #49: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.1089 KDa
SequenceString:
MEEKRNLRKE RIGVVTSNKM DKSIVISEVR KVKHPLYGKF VLKTKKYVAH DETNDCNIGD TVRISETRPL SKTKCWRLVE ILERAK

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #50: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 11.378475 KDa
SequenceString:
MSTIEQSAKG KKDGDIRYLT PLNIETNKTK KYCRFKKSGI KYIDYKDADF LLKFVNEQGK ILPRRLTGTS LKYQRKVSVA VKRARHLAL MPYVADLLK

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #51: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.278883 KDa
SequenceString:
MARSLKKGPF VHYKLDKKVQ ENVESGKNSV VKTWSRASMI TPDFVGQTIA VHNGRQFVPV YVTENMVGHK LGEFSPTRSF RGHAGAKNK GKK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #52: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 9.442211 KDa
SequenceString:
MANHKSALKR IRSNEKRRVL NRYQHKTTRN AIKALRLATD KSDAAAKLST VISMIDKLAK KNIIHDNKAS NLKSKLTKHV AKL

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #53: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.433809 KDa
SequenceString:
MLIIPIKDGE NIDRALKRYK RKFDKTGTVR QLRARTAFIK PSVVKRAQIQ KAAYIQTLKD SLES

UniProtKB: Small ribosomal subunit protein bS21

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Macromolecule #24: RNA (2862-MER)

MacromoleculeName: RNA (2862-MER) / type: rna / ID: 24 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 927.297188 KDa
SequenceString: AAAUAAGGGC GUAUGGGGAA UGCCUAGGCU CUCAGAGGCG AUGAAGGACG UGAUAAGCUG CGAAAAGCUG CGGGGACUGG CACACACAG AUUGAUCCGC AGAUAUCCGA AUGGGGCAAC CCGCUAUAUU GAAGAUAUAG CACACCGAUA GGUGGGCAAA C CCGCUGAA ...String:
AAAUAAGGGC GUAUGGGGAA UGCCUAGGCU CUCAGAGGCG AUGAAGGACG UGAUAAGCUG CGAAAAGCUG CGGGGACUGG CACACACAG AUUGAUCCGC AGAUAUCCGA AUGGGGCAAC CCGCUAUAUU GAAGAUAUAG CACACCGAUA GGUGGGCAAA C CCGCUGAA CUGAAACAUC UAAGUAGGCG GAGGAGAAGA AAACAAAAGU GAUUCCGUAA GUAGUGGCGA GCGAACGCGG AU UAGCCCA AACCAGUGCU GUUACGGCAG UGCUGGGGUU GUAGGACCAC GACAUUUUAU GCAUGCAGAA CCGGAAGUUA CUG GAAAGU GACGCCAAAG AGGGUGAUAG CCCCGUAUGG GUAAUAAAUG UAAUAGAUAG UGGUAUCCUG AGUAGGGCGG GACA CGUGA AACCCUGUCU GAAUUCGGCG GGACCAUCCG CUAAGGCUAA AUACUCCUGA GAGACCGAUA GUGAACCAGU ACCGU GAGG GAAAGGUGAA AAGAACCGUG AAUAACGGAG UGAAAUAGAU CCUGAAACCA UACGCUUACA AGCGGUCGGA GCCCUU UCG UGGGGUGACG GCGUGCCUUU UGCAUAAUGA GCCUACGAGU UAACGCUGCU GGCAAGGAUA AGUACUUCAG GUACGGA UC CGCAGCGAAA GCGAGUCUGA AUAGGGCGCU UUAGUCAGUA GUGUUAGACG CGAAACCGUG UGAUCUACCC AUGGGCAG G UUGAAGCUGU GGUAACACAC AGUGGAGGAC CGAACCGGUU GACGUUGAAA AGUCUUCGGA UGACCUGUGG GUAGGGGUG AAAGGCCAAU CAAACUCGGA AAUAGCUCGU ACUCCCCGAA AUGCAUUUAG GUGCAGCGUU AGUUAUAAAG UUAUACAGAG GUAGAGCUA CUGAUUGGAU GCGGGGGCUU CACCGCCCAC CAAUUCCUGA CAAACUCCGA AUGCUGUAUA AUGUUCACUA A CAGUGAGG GCUUGGGUGC UAAGGUCCAA GUCCGAGAGG GAAAGAACCC AGACCAUCAG CUAAGGUCCC CAAAUAUAUG CU AAGUUGA AAGAACGAGG UUUGUCUGCC CAGACAGCUA GGAUGUUGGC UUGGAAGCAG CCAUUCAUUU AAAGAGUGCG UAA CAGCUC ACUAGUCGAG CGGACGAGCA UGGAUAAUAA UCGGGCAUAA GCAUAUUACC GAAGCUAUGG AUUUGCAAGU AAUU GCAAG UGGUAGGGGA GCAUUCUAAC AGGGUUGAAG GUUUUUCGUA AGGAGGGCUG GACUGGUUAG AAAAGAAAAU GUAGG CAUA AGUAACGAUA AUGCGGGCGA GAAACCCGCA CACCGAAAAA CUAAGGUUUC CACAGCUAUG CUAAUCAGCU GUGGGU UAG UCUGGUCCUA AGGCGAACCC GAAAGGGACA GUCGAUGGCU AACGGGUUAA UAUUCCCGUA CUACUAAUUA CUGUGAU GG GGUGACGGAG UGAUGAAAGC GCCGCGAACU GACGGAAUAG UUCGUUGAAG UACCUAGCUA UAGGUCCUGU AGUAAAAU G CGCAGGAACU GGUGAAAUAC GAUAGUACUC GGAGUCUUCG GACAAAGAGA UAGUGCGCCU AAGGGCUUCC AAGAAAAAC CUCUAAACUU CAGGUAAUUA GUACCAGUAC CGUAAACCGA CACAGGUAGU UGAGGAGAGA AUCCUAAGGU GCUCGAGAUA UUCAUGGCU AAGGAAUUAG GCAAAAUAGA CCUGUAACUU CGGGAGAAAG GUCGCCCUGA GCAAUCAGGG CCGCAGUGAA G AGGUCCAG GCGACUGUUU AUCAAAAACA CAGGGCUCUG CAAAAUCGUA AGAUGAAGUA UAGGGCCUGA CACCUGCCCG GU GCUGGAA GGUUAAGAGG AGAUGUUAUC UUCGGAGAAG CAUUGAAUUG AAGCCCCAGU AAACGGCGGC CGUAACUAUA ACG GUCCUA AGGUAGCGAA AUUCCUUGUC GGGUAAGUUC CGACCUGCAC GAAUGGUGUA ACGAUCUGGA CACUGUCUCA GCCA UGAGC UCGGUGAAAU UGUAGUAACG GUGAAGAUGC CGUUUACCCG CAGUGGGACG AAAAGACCCU GUGCACCUUU ACUAU AGCU UAGUACUGAC CUUGGAUAAA UGAUGUGUAG GAUAGGUUGG AGACUAUGAA GCGGCGUCGC CAGGCGUUGU GGAGUC AUU GUUGAAAUAC AACCCUUUGU UUAUCUGAGG CCUAACCUCG CUAUGCGGGG GACAGUGCUU GGUGGGUAGU UUGACUG GG GUGGUCGCCU CCAAAAGAGU AACGGAGGCU UCUAAAGGUU CCCUCAGUAC GCUUGGUAAC CGUGCGUAGA GUGCAAUG G CAUAAGGGAG CUUGACUGAG AGACAUACAG GUCGAUCAGG UACGAAAGUA GAGCAUAGUG AUCCGGUGGU UCCGCAUGG AAGGGCCAUC GCUCAAAGGA UAAAAGGUAC GCCGGGGAUA ACAGGCUGAU CUCCCCCAAG AGUUCAUAUC GACGGGGGGG UUUGGCACC UCGAUGUCGG CUCGUCACAU CCUGGGGCUG GAGAAGGUCC CAAGGGUUGG GCUGUUCGCC CAUUAAAGUG G UACGCGAG CUGGGUUCAG AACGUCGUGA GACAGUUCGG UCUCUAUCUA CUGCGGGCGU UAGAAAUUUG AGUGGAUCUG AU UCUAGUA CGAGAGGACC GAAUUGGACU AACCUCUAGU GUAUCUGUUG UCCCGCCAGG GGCACCGCAG AGUAGCUACG UUG GGAAGG GAUAAGCGCU GAAAGCAUAU AAGCGCGAAA CCCACCACAA GAUGAGAUUU CUUUUAAGGA UCGUGGAAGA UGAC CACGU UGAUAGGCUA UAGAUGUAAA GGCAGUAAUG UCAUAGUCGA GUAGUACUAA UAAUCCG

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Macromolecule #25: RNA (1519-MER)

MacromoleculeName: RNA (1519-MER) / type: rna / ID: 25 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 491.83325 KDa
SequenceString: UAUACGAUGA AGAGUUUGAU CCUGGCUCAG GAUGAACGCU AGCGGCAGGC UUAACACAUG CAAGUCGAGG GGUAGAAGUC UUCGGGCUU UGAGACCGGC GCACGGGUGC GUAACGCGUA UGCAAUCUGC CUUUCACAGA GGGAUAGCCC AGAGAAAUUU G GAUUAAUA ...String:
UAUACGAUGA AGAGUUUGAU CCUGGCUCAG GAUGAACGCU AGCGGCAGGC UUAACACAUG CAAGUCGAGG GGUAGAAGUC UUCGGGCUU UGAGACCGGC GCACGGGUGC GUAACGCGUA UGCAAUCUGC CUUUCACAGA GGGAUAGCCC AGAGAAAUUU G GAUUAAUA CCUCAUAGUA UUAUAGAGCG GCAUCACUUU AUAAUUAAAG UCACAACGGU GAAAGAUGAG CAUGCGUCCC AU UAGCUAG UUGGUAAGGU AACGGCUUAC CAAGGCCACG AUGGGUAGGG GUCCUGAGAG GGAGAUCCCC CACACUGGUA CUG AGACAC GGACCAGACU CCUACGGGAG GCAGCAGUGA GGAAUAUUGG UCAAUGGACG CAAGUCUGAA CCAGCCAUGC CGCG UGCAG GAUGACGGUC CUAUGGAUUG UAAACUGCUU UUGUACGAGA AGAAACACUC CUAUAUAUAG GAGCUUGACG GUAUC GUAA GAAUAAGGAU CGGCUAACUC CGUGCCAGCA GCCGCGGUAA UACGGAGGAU CCAAGCGUUA UCCGGAAUCA CUGGGU UUA AAGGGUCCGU AGGCGGUUUA GUAAGUCAGU GGUGAAAGCC CAUCGCUCAA CGGUGGAACG GCCAUUGAUA CUGCUGA AC UUGAAUUACU GGGAAGUAAC UAGAAUAUGU AGUGUAGCGG UGAAAUGCUU AGAUAUUACA UGGAAUACCA AUUGCGAA G GCAGGUUACU ACCAGUUUAU UGACGCUGAU GGACGAAAGC GUGGGGAGCG AACAGGAUUA GAUACCCUGG UAGUCCACG CCGUAAACGA UGGAUACUAG CUGUUGGGCG CAAGUUCAGU GGCUAAGCGA AAGUGAUAAG UAUCCCACCU GGGGAGUACG AACGCAAGU UUGAAACUCA AAGGAAUUGA CGGGGGCCCG CACAAGCGGU GGAACAUGUG GUUUAAUUCG AUGAUACGCG A GGAACCUU ACCAAGGCUU AAAUGCAGAC UGACCGAUUU GGAAACAGAU CUUAUCGCAA GACAGUUUAC AAGGUGCUGC AU GGUUGUC GUCAGCUCGU GCCGUGAGGU GUCAGGUUAA GUCCUAUAAC GAGCGCAACC CCUGUUGUUA GUUGCCAGCG AGU GAUGUC GGGAACUCUA ACAAGACUGC CAGUGCAAAC UGUGAGGAAG GUGGGGAUGA CGUCAAAUCA UCACGGCCCU UACG CCUUG GGCUACACAC GUGCUACAAU GGCCGGUACA GAGAGCAGCC ACUGGGUGAC CAGGAGCGAA UCUAUAAAGC CGGUC ACAG UUCGGAUCGG AGUCUGCAAC UCGACUCCGU GAAGCUGGAA UCGCUAGUAA UCGGAUAUCA GCCAUGAUCC GGUGAA UAC GUUCCCGGGC CUUGUACACA CCGCCCGUCA AGCCAUGGAA GCUGGGGGUG CCUGAAGUCG GUGACCGCAA GGAGCUG CC UAGGGUAAAA CUGGUAACUA GGGCUAAGUC GUAACAAGGU AGCCGUACCG GAAGGUGCGG CUGGAACACC UCCUUUCU

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Macromolecule #26: RNA (111-MER)

MacromoleculeName: RNA (111-MER) / type: rna / ID: 26 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 35.661992 KDa
SequenceString:
UUAAGGUGGU UAUUGCGGCG GGGCUCACCU CUUCCCAUCC CGAACAGAGA AGUUAAGCCC GCUUGCGCAG AUGGUACUGC AGUUUUGUG GGAGAGUAUG UCGUCGCCUU UC

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Macromolecule #54: water

MacromoleculeName: water / type: ligand / ID: 54 / Number of copies: 1 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1314417
Startup modelType of model: EMDB MAP
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 705543
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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